DOCSLIB.ORG

APHA Parasitology Group: Annual Review of Literature and Horizon Scanning Report 2018

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
APHA Parasitology Group: Annual Review of Literature and Horizon Scanning Report 2018 APHA Parasitology Group Annual Review of Literature and Horizon Scanning Report 2018 Published: November 2019 November 2019 © Crown copyright 2019 You may re-use this information (excluding logos) free of charge in any format or medium, under the terms of the Open Government Licence v.3. To view this licence visit www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ or email [email protected] This publication is available at www.gov.uk/government/publications Any enquiries regarding this publication should be sent to us at [email protected] Year of publication: 2019 The Animal and Plant Health Agency (APHA) is an executive agency of the Department for Environment, Food & Rural Affairs, and also works on behalf of the Scottish Government and Welsh Government. October 2018 Contents Summary ............................................................................................................................. 1 Fasciola hepatica ............................................................................................................. 1 Rumen fluke (Calicophoron daubneyi) ............................................................................. 2 Parasitic gastro-enteritis (PGE) ........................................................................................ 2 Anthelmintic resistance .................................................................................................... 4 Cestodes .......................................................................................................................... 4 Ectoparasites ................................................................................................................... 5 Vectors and vector-borne diseases .................................................................................. 5 Abstracts .............................................................................................................................. 5 Fasciola hepatica ................................................................................................................. 5 Epidemiology .................................................................................................................. 10 Non chemical control of Fasciola hepatica ..................................................................... 13 Vaccination ..................................................................................................................... 13 Chemical control of Fasciola .......................................................................................... 16 Diagnosis ....................................................................................................................... 19 Rumen fluke (Calicophoron daubneyi) ............................................................................... 24 Parasitic gastro-enteritis (PGE) ......................................................................................... 26 Diagnosis of PGE ........................................................................................................... 27 Vaccination ..................................................................................................................... 28 PGE Control ................................................................................................................... 29 Parasite immunity ........................................................................................................... 34 Haemonchus contortus ...................................................................................................... 35 Vaccination ..................................................................................................................... 37 Immunity ......................................................................................................................... 39 Molecular studies ........................................................................................................... 40 October 2018 Diagnosis ....................................................................................................................... 42 Anthelmintics ..................................................................................................................... 43 Anthelmintic resistance ...................................................................................................... 46 Parasites of cattle .............................................................................................................. 60 Parasitism and dairy sheep ................................................................................................ 63 Parasitism of dairy goats .................................................................................................... 64 Alternative control of parasites ........................................................................................... 65 Plant based products...................................................................................................... 65 Fungi .............................................................................................................................. 71 Miscellaneous products .................................................................................................. 73 Dictyocaulus spp. ............................................................................................................... 74 Cestodes and other zoonotic parasites .............................................................................. 76 Ectoparasites ..................................................................................................................... 83 Bovine besnoitiosis ............................................................................................................ 87 Neospora caninum ............................................................................................................. 90 Vector borne diseases: ...................................................................................................... 92 Tick borne infections and wild and domestic hosts: ....................................................... 98 Poultry mites .................................................................................................................... 110 Wildlife ............................................................................................................................. 111 Companion animals ......................................................................................................... 112 October 2018 Summary Fasciola hepatica Research into various vaccination candidates and also why research to date has not produced an effective commercialised vaccine continues. Various studies were also published into many new chemicals (some plant derived), adjuvants and routes of vaccination that appear to have potential A study to assist vaccine development showed that a genetic clonal expansion of F hepatica occurs within G. truncatula, highlighting the potential for amplification of drug resistant genotypes. Interestingly in one experiment, a single miracidium infecting a snail, produced over 3000 metacercariae during the snail’s lifetime, showing the potent multiplication step in this intermediate host. The effect of F hepatica on infected hosts’ global immune response has continued to be studied. It is modulated toward a nonproliferative type 2 state following natural challenge with F. hepatica. This has implications in terms of the timing of the administration of vaccination programs and for host susceptibility to coinfecting pathogens. A paper was published which cautiously suggested a link between F. hepatica infection and the shedding of E. coli O157 in cattle. Newer areas of research were also published. In Cuba (and elsewhere but not reported in the UK) the snail Pseudosuccinea columella acts as intermediate host of F. hepatica, Interestingly in Cuba there are populations of susceptible and naturally-resistant populations to F. hepatica within this snail species. Resistance in P. columella is characterized by the encapsulation of the parasite by host's immune cells. The effectiveness of P. columella resistance to F. hepatica does not decline with increasing parasite doses, successive infection or different geographical origins of parasite isolates, Understanding the peculiarities of the P. columella-F. hepatica interaction and the extent of the resistant phenotype is crucial for an effective parasite control and for developing alternatives to tackle fasciolosis transmission. A study was published to determine the effectiveness of Chaetogaster limnaei (a freshwater worm) as a natural controller of Fasciola hepatica in laboratory conditions. It is naturally symbiotically associated with lymnaeid snails as Galba truncatula with this association considered a preventative mechanism against trematode miracidia infection. An effective 70% natural control of Fasciola hepatica in Galba truncatula was observed, in vitro. Study in dairy herds in Denmark resulted in information pertinent for that country and noted that for diagnosis of infection the serum antibody ELISA was able to detect infection first, whereas both copro-antigen ELISA and faecal egg counts tended to increase in the same animals at a later point. Other diagnostic techmiques such as real time PCR and loop-mediated isothermal amplification (LAMP) have been shown to be able to detect F hepatica DNA from as early as one week post
Load more

Recommended publications
  • ADME and Pharmacokinetic Properties of Remdesivir: Its Drug Interaction Potential
    pharmaceuticals Review ADME and Pharmacokinetic Properties of Remdesivir: Its Drug Interaction Potential Subrata Deb * , Anthony Allen Reeves, Robert Hopefl and Rebecca Bejusca Department of Pharmaceutical Sciences, College of Pharmacy, Larkin University, Miami, FL 33169, USA; [email protected] (A.A.R.); [email protected] (R.H.); [email protected] (R.B.) * Correspondence: [email protected]; Tel.: +224-310-7870 Abstract: On 11 March 2020, the World Health Organization (WHO) classified the Coronavirus Disease 2019 (COVID-19) as a global pandemic, which tested healthcare systems, administrations, and treatment ingenuity across the world. COVID-19 is caused by the novel beta coronavirus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Since the inception of the pandemic, treatment options have been either limited or ineffective. Remdesivir, a drug originally designed to be used for Ebola virus, has antiviral activity against SARS-CoV-2 and has been included in the COVID-19 treatment regimens. Remdesivir is an adenosine nucleotide analog prodrug that is metabolically activated to a nucleoside triphosphate metabolite (GS-443902). The active nucleoside triphosphate metabolite is incorporated into the SARS-CoV-2 RNA viral chains, preventing its replication. The lack of reported drug development and characterization studies with remdesivir in public domain has created a void where information on the absorption, distribution, metabolism, elimination (ADME) properties, pharmacokinetics (PK), or drug-drug interaction (DDI) is limited. By Citation: Deb, S.; Reeves, A.A.; understanding these properties, clinicians can prevent subtherapeutic and supratherapeutic levels of Hopefl, R.; Bejusca, R. ADME and remdesivir and thus avoid further complications in COVID-19 patients. Remdesivir is metabolized Pharmacokinetic Properties of by both cytochrome P450 (CYP) and non-CYP enzymes such as carboxylesterases.
    [Show full text]
  • Tuesday June 2 Wednesday June 3
    Tuesday June 2 14:00- Registration 18:30 19:30 Welcome reception Wednesday June 3 08:00- Registration 09:00 09:00- Welcome and Introduction 09:15 09:15- Targeting Ebola Chair: Steven Kern 10:45 09:15- Conducting clinical trials in challenging Steven Kern 09:30 environments 09:30- France Estimating an effective dose for a repurposed 09:55 Mentré drug to treat Ebola: the case of favipiravir Estimating an effective dose for a new drug to 09:55- Matthias treat Ebola with incomplete information: the 10:20 Machacek case of Zmapp 10:20- An Adaptive Platform Trial for Ebola: Scott Berry 10:45 Application to Future Epidemics 10:45- Coffee break, Poster and Software session I 12:15 Posters in Group I (with poster numbers starting with I-) are accompanied by their presenter 12:15- Diabetes Chair: IñakiTrocóniz 12:55 Page | 1 12:15- Roberto A model of glucose clearance to improve the 12:35 Bizzotto description of glucose homeostasis A longitudinal HbA1c model elucidates genes 12:35- Rada Savic linked to disease progression on metformin 12:55 therapy 12:55- Lunch 14:25 On the 20th anniversary of 'Nonlinear 14:25- Chair: France models for repeated measurement 15:15 Mentré data' 14:25- David Why write a book in 1995 on nonlinear mixed 14:50 Giltinan effects modeling? 14:50- Marie Subsequent developments in nonlinear mixed 15:15 Davidian effects modeling 15:15- Tea break, Poster and Software session II 16:40 Posters in Group II (with poster numbers starting with II-) are accompanied by their presenter 16:40- Other diseases Chair: Ana Ruiz 17:40 José
    [Show full text]
  • CAS Number Index
    2334 CAS Number Index CAS # Page Name CAS # Page Name CAS # Page Name 50-00-0 905 Formaldehyde 56-81-5 967 Glycerol 61-90-5 1135 Leucine 50-02-2 596 Dexamethasone 56-85-9 963 Glutamine 62-44-2 1640 Phenacetin 50-06-6 1654 Phenobarbital 57-00-1 514 Creatine 62-46-4 1166 α-Lipoic acid 50-11-3 1288 Metharbital 57-22-7 2229 Vincristine 62-53-3 131 Aniline 50-12-4 1245 Mephenytoin 57-24-9 1950 Strychnine 62-73-7 626 Dichlorvos 50-23-7 1017 Hydrocortisone 57-27-2 1428 Morphine 63-05-8 127 Androstenedione 50-24-8 1739 Prednisolone 57-41-0 1672 Phenytoin 63-25-2 335 Carbaryl 50-29-3 569 DDT 57-42-1 1239 Meperidine 63-75-2 142 Arecoline 50-33-9 1666 Phenylbutazone 57-43-2 108 Amobarbital 64-04-0 1648 Phenethylamine 50-34-0 1770 Propantheline bromide 57-44-3 191 Barbital 64-13-1 1308 p-Methoxyamphetamine 50-35-1 2054 Thalidomide 57-47-6 1683 Physostigmine 64-17-5 784 Ethanol 50-36-2 497 Cocaine 57-53-4 1249 Meprobamate 64-18-6 909 Formic acid 50-37-3 1197 Lysergic acid diethylamide 57-55-6 1782 Propylene glycol 64-77-7 2104 Tolbutamide 50-44-2 1253 6-Mercaptopurine 57-66-9 1751 Probenecid 64-86-8 506 Colchicine 50-47-5 589 Desipramine 57-74-9 398 Chlordane 65-23-6 1802 Pyridoxine 50-48-6 103 Amitriptyline 57-92-1 1947 Streptomycin 65-29-2 931 Gallamine 50-49-7 1053 Imipramine 57-94-3 2179 Tubocurarine chloride 65-45-2 1888 Salicylamide 50-52-2 2071 Thioridazine 57-96-5 1966 Sulfinpyrazone 65-49-6 98 p-Aminosalicylic acid 50-53-3 426 Chlorpromazine 58-00-4 138 Apomorphine 66-76-2 632 Dicumarol 50-55-5 1841 Reserpine 58-05-9 1136 Leucovorin 66-79-5
    [Show full text]
  • Current Therapeutic Applications and Pharmacokinetic Modulations of Ivermectin
    Veterinary World, EISSN: 2231-0916 REVIEW ARTICLE Available at www.veterinaryworld.org/Vol.12/August-2019/5.pdf Open Access Current therapeutic applications and pharmacokinetic modulations of ivermectin Khan Sharun1, T. S. Shyamkumar2, V. A. Aneesha2, Kuldeep Dhama3, Abhijit Motiram Pawde1 and Amar Pal1 1. Division of Surgery, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India; 2. Division of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India; 3. Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India. Corresponding author: Khan Sharun, e-mail: [email protected] Co-authors: TSS: [email protected], VAA: [email protected], KD: [email protected], AMP: [email protected], AP: [email protected] Received: 17-05-2019, Accepted: 29-06-2019, Published online: 08-08-2019 doi: 10.14202/vetworld.2019.1204-1211 How to cite this article: Sharun K, Shyamkumar TS, Aneesha VA, Dhama K, Pawde AM, Pal A (2019) Current therapeutic applications and pharmacokinetic modulations of ivermectin, Veterinary World, 12(8): 1204-1211. Abstract Ivermectin is considered to be a wonder drug due to its broad-spectrum antiparasitic activity against both ectoparasites and endoparasites (under class of endectocide) and has multiple applications in both veterinary and human medicine. In particular, ivermectin is commonly used in the treatment of different kinds of infections and infestations. By altering the vehicles used in the formulations, the pharmacokinetic properties of different ivermectin preparations can be altered. Since its development, various vehicles have been evaluated to assess the efficacy, safety, and therapeutic systemic concentrations of ivermectin in different species. A subcutaneous route of administration is preferred over a topical or an oral route for ivermectin due to superior bioavailability.
    [Show full text]
  • Parasitology Group Annual Review of Literature and Horizon Scanning Report 2018
    APHA Parasitology Group Annual Review of Literature and Horizon Scanning Report 2018 Published: November 2019 November 2019 © Crown copyright 2018 You may re-use this information (excluding logos) free of charge in any format or medium, under the terms of the Open Government Licence v.3. To view this licence visit www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ or email [email protected] This publication is available at www.gov.uk/government/publications Any enquiries regarding this publication should be sent to us at [email protected] Year of publication: 2019 The Animal and Plant Health Agency (APHA) is an executive agency of the Department for Environment, Food & Rural Affairs, and also works on behalf of the Scottish Government and Welsh Government. November 2019 Contents Summary ............................................................................................................................. 1 Fasciola hepatica ............................................................................................................. 1 Rumen fluke (Calicophoron daubneyi) ............................................................................. 2 Parasitic gastro-enteritis (PGE) ........................................................................................ 2 Anthelmintic resistance .................................................................................................... 4 Cestodes .........................................................................................................................
    [Show full text]
  • Patent Application Publication ( 10 ) Pub . No . : US 2019 / 0192440 A1
    US 20190192440A1 (19 ) United States (12 ) Patent Application Publication ( 10) Pub . No. : US 2019 /0192440 A1 LI (43 ) Pub . Date : Jun . 27 , 2019 ( 54 ) ORAL DRUG DOSAGE FORM COMPRISING Publication Classification DRUG IN THE FORM OF NANOPARTICLES (51 ) Int . CI. A61K 9 / 20 (2006 .01 ) ( 71 ) Applicant: Triastek , Inc. , Nanjing ( CN ) A61K 9 /00 ( 2006 . 01) A61K 31/ 192 ( 2006 .01 ) (72 ) Inventor : Xiaoling LI , Dublin , CA (US ) A61K 9 / 24 ( 2006 .01 ) ( 52 ) U . S . CI. ( 21 ) Appl. No. : 16 /289 ,499 CPC . .. .. A61K 9 /2031 (2013 . 01 ) ; A61K 9 /0065 ( 22 ) Filed : Feb . 28 , 2019 (2013 .01 ) ; A61K 9 / 209 ( 2013 .01 ) ; A61K 9 /2027 ( 2013 .01 ) ; A61K 31/ 192 ( 2013. 01 ) ; Related U . S . Application Data A61K 9 /2072 ( 2013 .01 ) (63 ) Continuation of application No. 16 /028 ,305 , filed on Jul. 5 , 2018 , now Pat . No . 10 , 258 ,575 , which is a (57 ) ABSTRACT continuation of application No . 15 / 173 ,596 , filed on The present disclosure provides a stable solid pharmaceuti Jun . 3 , 2016 . cal dosage form for oral administration . The dosage form (60 ) Provisional application No . 62 /313 ,092 , filed on Mar. includes a substrate that forms at least one compartment and 24 , 2016 , provisional application No . 62 / 296 , 087 , a drug content loaded into the compartment. The dosage filed on Feb . 17 , 2016 , provisional application No . form is so designed that the active pharmaceutical ingredient 62 / 170, 645 , filed on Jun . 3 , 2015 . of the drug content is released in a controlled manner. Patent Application Publication Jun . 27 , 2019 Sheet 1 of 20 US 2019 /0192440 A1 FIG .
    [Show full text]
  • The New Anthelmintic Tribendimidine Is an L-Type (Levamisole and Pyrantel) Nicotinic Acetylcholine Receptor Agonist
    The New Anthelmintic Tribendimidine is an L-type (Levamisole and Pyrantel) Nicotinic Acetylcholine Receptor Agonist Yan Hu1, Shu-Hua Xiao2, Raffi V. Aroian1* 1 Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California, United States of America, 2 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, People’s Republic of China Abstract Background: Intestinal parasitic nematodes such as hookworms, Ascaris lumbricoides, and Trichuris trichiura are amongst most prevalent tropical parasites in the world today. Although these parasites cause a tremendous disease burden, we have very few anthelmintic drugs with which to treat them. In the past three decades only one new anthelmintic, tribendimidine, has been developed and taken into human clinical trials. Studies show that tribendimidine is safe and has good clinical activity against Ascaris and hookworms. However, little is known about its mechanism of action and potential resistance pathway(s). Such information is important for preventing, detecting, and managing resistance, for safety considerations, and for knowing how to combine tribendimidine with other anthelmintics. Methodology/Principal Findings: To investigate how tribendimidine works and how resistance to it might develop, we turned to the genetically tractable nematode, Caenorhabditis elegans. When exposed to tribendimidine, C. elegans hermaphrodites undergo a near immediate loss of motility; longer exposure results in extensive body damage, developmental arrest, reductions in fecundity, and/or death. We performed a forward genetic screen for tribendimidine- resistant mutants and obtained ten resistant alleles that fall into four complementation groups. Intoxication assays, complementation tests, genetic mapping experiments, and sequencing of nucleic acids indicate tribendimidine-resistant mutants are resistant also to levamisole and pyrantel and alter the same genes that mutate to levamisole resistance.
    [Show full text]
  • In Vivo and in Vitro Studies of Cry5b and Nicotinic Acetylcholine Receptor Agonist Anthelmintics Reveal a Powerful and Unique Co
    University of Massachusetts Medical School eScholarship@UMMS Open Access Articles Open Access Publications by UMMS Authors 2018-05-18 In vivo and in vitro studies of Cry5B and nicotinic acetylcholine receptor agonist anthelmintics reveal a powerful and unique combination therapy against intestinal nematode parasites Yan Hu University of Massachusetts Medical School Et al. Let us know how access to this document benefits ou.y Follow this and additional works at: https://escholarship.umassmed.edu/oapubs Part of the Digestive System Diseases Commons, Microbiology Commons, Parasitic Diseases Commons, Therapeutics Commons, and the Tropical Medicine Commons Repository Citation Hu Y, Miller M, Zhang B, Nguyen T, Nielsen MK, Aroian RV. (2018). In vivo and in vitro studies of Cry5B and nicotinic acetylcholine receptor agonist anthelmintics reveal a powerful and unique combination therapy against intestinal nematode parasites. Open Access Articles. https://doi.org/10.1371/ journal.pntd.0006506. Retrieved from https://escholarship.umassmed.edu/oapubs/3470 Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 License. This material is brought to you by eScholarship@UMMS. It has been accepted for inclusion in Open Access Articles by an authorized administrator of eScholarship@UMMS. For more information, please contact [email protected]. RESEARCH ARTICLE In vivo and in vitro studies of Cry5B and nicotinic acetylcholine receptor agonist anthelmintics reveal a powerful and unique combination therapy against intestinal
    [Show full text]
  • PRODUCT INFORMATION Derquantel Item No
    PRODUCT INFORMATION Derquantel Item No. 19441 CAS Registry No.: 187865-22-1 Formal Name: (1’R,5’aS,7’R,8’aS,9’aR)-2’,3’,8’a,9,9’,10- hexahydro-1’-hydroxy-1’,4,4,8’,8’,11’- hexamethyl-spiro[4H,8H-[1,4]dioxepino[2,3-g] O indole-8,7’(8’H)-[5H,6H-5a,9a](iminomethano) [1H]cyclopent[f]indolizin]-10’-one Synonyms: PF-00520904, PNU 141962, O 2-deoxy-Paraherquamide N N MF: C28H37N3O4 FW: 479.6 N O H Purity: ≥98% H OH Supplied as: A solid Storage: -20°C Stability: ≥2 years Information represents the product specifications. Batch specific analytical results are provided on each certificate of analysis. Laboratory Procedures Derquantel is supplied as a solid. A stock solution may be made by dissolving the derquantel in the solvent of choice. Derquantel is soluble in organic solvents such as ethanol, methanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. Derquantel is sparingly soluble in aqueous solutions. To enhance aqueous solubility, dilute the organic solvent solution into aqueous buffers or isotonic saline. If performing biological experiments, ensure the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day. Description Derquantel is an anthelmintic spiroindole that acts as a nicotinic acetylcholine receptor antagonist, causing flaccid paralysis and expulsion of nematodes.1 A combination of derquantel and the macrocyclic lactone, abamectin (Item No. 19201), has been found to have synergistic anthelmintic effects against gastrointestinal nematode parasites in sheep.2 References 1.
    [Show full text]
  • Haemonchus Contortus ABC Transporters Linked to Macrocyclic
    Haemonchus contortus ABC transporters linked to Macrocyclic lactone resistance Pablo Godoy Rosas Institute of Parasitology, McGill University Montreal, CANADA March 2015 A thesis submitted to Faculty of Agricultural and Environmental Sciences, McGill University in partial fulfillment with the requirements of the degree of Doctor of Philosophy © Pablo Godoy 2015 0 Abstract. Haemonchus contortus is a parasitic nematode distributed all around the world where ruminants graze and it causes serious decreases in small ruminants production. In order to control this veterinary helminth as well as others, the administration of anthelmintic chemotherapeutics is still the main strategy used to overcome parasitosis in veterinary medicine. One particular anthelmintic class is the macrocyclic lactones (MLs), which since their launch more than thirty years ago have shown remarkable efficacy against endo and ectoparasites, being successful against parasitic pathogens resistant to older parasiticide drugs. Currently, they represent the cornerstone of antiparasite chemotherapy in animal health and are also important in humans. Unfortunately, due to their outstanding effects, the continuous use of the MLs against parasitic nematodes, including H. contortus, has led to drug resistance, and now limits their use. In H. contortus, one of the resistance mechanisms involved could be the over expression of P- glycoproteins (PGPs) and possibly other nematode transporters, homologs of multidrug resistance (MDR) pumps in mammals which belong to the ATP-Binding- Cassette (ABC) transporter protein family. The activity of the ABC transporters is based on the hydrolysis of ATP coupling to the extrusion of many unrelated compounds across the membrane. In the host, these plasma membrane proteins are expressed in different organs and tissues such as intestine, liver, blood-brain- barreer (BBB) and kidney, influencing the bioavailability and pharmacokinetics of different substrates including the ML drugs.
    [Show full text]
  • Activation of Caenorhabditis Elegans Levamisole-Sensitive and Mammalian Nicotinic Receptors by the Antiparasitic Bephenium
    Molecular Pharmacology Fast Forward. Published on September 6, 2018 as DOI: 10.1124/mol.118.113357 This article has not been copyedited and formatted. The final version may differ from this version. MOL#113357 Title Page Activation of Caenorhabditis elegans levamisole-sensitive and mammalian nicotinic receptors by the antiparasitic bephenium Downloaded from molpharm.aspetjournals.org Ornella Turani, Guillermina Hernando, Jeremías Corradi, and Cecilia Bouzat at ASPET Journals on September 27, 2021 Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bahía Blanca, Argentina. 1 Molecular Pharmacology Fast Forward. Published on September 6, 2018 as DOI: 10.1124/mol.118.113357 This article has not been copyedited and formatted. The final version may differ from this version. MOL#113357 Running Title Page a) Running Title: bephenium action at nAChRs b) Corresponding author: Cecilia Bouzat. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Camino La Carrindanga Km 7. 8000 Bahía Blanca. Argentina. E-mail: [email protected]. Telephone: 54-291-4861201- FAX: 54-291-4861200 Downloaded from c) Number of text pages: 36 Number of Figures: 5 Number of References: 56 molpharm.aspetjournals.org Number of words in the Abstract: 248 Number of words in the Introduction: 672 Number of words in the Discussion: 1448 at ASPET Journals on September 27, 2021 d) Nonstandard abbreviations: ACh, acetylcholine; BBE, best binding energy; L-AChR, levamisole-sensitive nAChR; nAChR, nicotinic acetylcholine receptor. 2 Molecular Pharmacology Fast Forward. Published on September 6, 2018 as DOI: 10.1124/mol.118.113357 This article has not been copyedited and formatted.
    [Show full text]
  • Đorđe S. Marjanović Doktor Veterinarske Medicine
    UNIVERZITET U BEOGRADU FAKULTET VETERINARSKE MEDICINE Katedra za Farmakologiju i toksikologiju Đorđe S. Marjanović Doktor veterinarske medicine Ispitivanje mehanizma antinematodnog dejstva odabranih monoterpenoidnih i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja Doktorska disertacija Beograd, 2019 UNIVERZITET U BEOGRADU FAKULTET VETERINARSKE MEDICINE Katedra za Farmakologiju i toksikologiju Đorđe S. Marjanović Doktor veterinarske medicine Ispitivanje mehanizma antinematodnog dejstva odabranih monoterpenoidnih i diterpenoidnih aktivnih sastojaka esencijalnih biljnih ulja Doktorska disertacija Beograd, 2019 Đorđe S. Marjanović Doctor of veterinary medicine Investigation of the antinematodal mechanism of action of selected monotherpenoid and ditherpenoids active ingredients from plant essential oils Doctoral dissertation Belgrade, 2019 Mentor: dr Saša M. Trailović, redovni profesor Fakultet veterinarske medicine Univerziteta u Beogradu Komisija za odbranu: dr Saša M. Trailović, redovni profesor Fakultet veterinarske medicine Univerziteta u Beogradu dr Mirjana Milovanović, vanredni profesor Fakultet veterinarske medicine Univerziteta u Beogradu dr Zoran Todorović, redovni profesor Medicinski fakultet Univerziteta u Beogradu Mesto i datum odbrane: Beograd, ______________ Zahvalnica Za pruženu šansu za rad u Farmakologiji želim da izrazim najiskreniju zahvalnost svom mentoru prof. dr Saši M. Trailoviću bez koga ne bih kročio u svet nauke, koji me je prihvatio na svoje istraživanje i projekat kojim rukovodi. Za sve što sam naučio
    [Show full text]