Mario Izaguirre-Sierra, PhD

The University of North Carolina at Chapel Hill Department of Biology and Genetics Campus Box 3280 Chapel Hill, North Carolina 27599 [email protected] [email protected]

Date of Birth: November 3th, 1977 Marital Status: Single Nationality: Mexican Office: 418 Fordham Hall Office number: (919) 843-7991

Professional career

•June 2008 – Present. Postdoc at The Department of Biology and Genetics. The University of North Carolina at Chapel Hill. Chapel Hill, NC. USA. Project title: “Coilin, Cajal bodies and Spinal Muscular Atrophy” Supervisors: Professor Gregory Matera and Dr. Greg Copenhaver

•January 2008-May 2008. Postdoc at the Cell and Department, The (JIC), , UK. Supervisors: Professor Liam Dolan and Professor Peter Shaw.

•PhD in Cell and Developmental Biology (2003-2008) at the John Innes Centre (JIC) and University of East Anglia (UEA), Norwich, UK. Funded by the National Council for Science and Technology (Consejo Nacional de Ciencia y Tecnologia-CONACYT), Mexican Government.

PhD project title: “Cajal body mutants in : Poly Cajal Body, a ULP1 cysteine protease required for the maintenance of Cajal body integrity” Supervisors: Professor Liam Dolan and Professor Peter Shaw, Cell and Developmental Biology Department, JIC.

•Bachelor in Biology. Research thesis project (2000-2002) at the Ecology Institute, National Autonomous University of Mexico-UNAM. Mexico City. Mexico. Project title: “Subcellular localisation of a LRR protein encoded by FLOR1” Supervisors: Dra. Elena Alvarez-Buylla Roces and Dra. Francisca Acevedo Gasman, Ecology Institute, UNAM.

Academic background and Honors/Awards

•2006 Cell Imaging Techniques Course at Brookes University. Oxford, UK. Sponsored by the Royal Microscopical Society and the Gatsby Charitable Foundation.

•1996-2001 Bachelor of Science in Biology at Facultad de Ciencias, UNAM, Mexico City. Mexico.

1 •2000 (SUMMER) Undergraduate Research Program (URP) at Cold Spring Harbor Laboratory (CSHL), NY. USA. Sponsored by CSHL and the Glass & Libby Foundations. Project title "Does Actin Play a Role in Nuclear Structure?" Supervisor: Professor David Spector

•1999 IX Scientific Research Summer Course at Ecology Institute, UNAM. Mexico City. Sponsored by Mexican Academy of Sciences. Supervisors: Dra. Elena Alvarez-Buylla Roces and Dra. Francisca Acevedo Gasman, Ecology Institute, UNAM.

•1998 Course of Basic Molecular Biology Techniques at Biological Research Center (CIBNOR), La Paz, Baja California, Mexico. Supervisor: Dra. Norma Hernandez Saavedra.

Teaching and Management of Students

•From March 2009-present: Co-Supervised with Prof. Greg Matera. Michael Murphy. Junior Biology Major at UNC at Chapel Hill. USA. Project title: Characterisation of SMN mutants in Arabidopsis thaliana.

•From August 2008 - present: Co-Supervised with Prof. Greg Matera. Suzanne Ploeger. Senior Honours Thesis Student. Biology Major at UNC at Chapel Hill. USA. Project title: Understanding the molecular basis of SmnE33 mutation in D. melanogaster.

•2001. Biology for High School Students at Colegio Nacional de Matematicas (CONAMAT). Calz. Ermita Iztapalapa 2914 Col. los Ángeles. Mexico City.

Conferences and Oral Presentations

•26th January 2009. Duke University. Duke Arabidopsis Forum. Durham, NC. USA. Oral presentation "A Genetic Approach to Dissect Cajal Body Structure and Function" Invited by Dr. Meng Chen.

•1st April 2008. Universidad de Malaga, Departamento de Biología Celular, Genética y Fisiología, Malaga, Spain. Oral presentation : “Cajal body mutants in Arabidopsis thaliana” Invited by Prof. Miguel Angel Botella and Dr. David Pose.

•1st -5th September 2007. EMBO Conference on Nuclear Structure and Dynamics. Montpellier, France. Selected Oral presentation. “Poly Cajal Body, a ULP cysteine protease involved in the regulation of Cajal Body number”.

•June 2006. John Innes Centre, Cell and Developmental Biology Departmental Seminar. Norwich, UK. Oral Presentation. “Cajal bodies Wrestle SUMO to maintain Nuclear Architecture”

•24th -28th September 2005. EMBO/FEBS Conference on Nuclear Structure and Dynamics. La Grande Motte, Montpellier, France. Selected Poster presentation. “Characterisation of the role of PCB in Cajal body formation”

2 Technical Skills

Protein, Cloning and Molecular Biology Techniques. Immunofluorescence and In situ Hybridisation in Plant sections and animal Cell Culture (Mammalian and Drosophila), whole-mount immunofluorescence in planta and in Drosophila. Electron Microscopy, Confocal Microscopy, use of CCD camera and Spinning disc microscopes.

Publications

•1- M. Izaguirre-Sierra, The Nucleolus. In Biology of the Cell Nucleus. Ontiveros- Llamas y Echeverria edits. Las Prensas de Ciencias editorial UNAM. 2001, MEXICO CITY. ISBN: 968-36-9155-2. (Scanned and attached in a different email)

•2- Francisca G. Acevedo, Alicia Gamboa, Julio Paez-Valencia, Luis Felipe Jimenez-Garcia, Mario Izaguirre-Sierra, and Elena R. Alvarez-Buylla. 2004. FLOR1, a putative interaction partner of the floral homeotic protein AGAMOUS, is a plant-specific intracellular LRR. Plant Science 167(2): 225-231. (Attached)

3 Manuscripts in preparation.

•M. Izaguirre-Sierra and A.G. Matera. Assembly and Function of Nuclear bodies. Invited review in the special issue of The Cell biology of the Nucleus. Developmental Cell. To be submitted May 2009 and published end 2009.

•M. Izaguirre-Sierra, P. Shaw and L. Dolan. Poly Cajal Body, a ULP1 cysteine protease required for the maintenance of Cajal body integrity. To be submitted.

•M. Izaguirre-Sierra*, E. Havecker*, D Baulcombe, P. Shaw and L. Dolan. Cajal Body mutants in Arabidopsis thaliana uncover a POL IV transcript. To be submitted (* Equal Contribution).

Additional Information

Languages: Spanish native language, 100% English writing and speaking.

References

Professor Liam Dolan Department of Cell and Developmental Biology John Innes Centre Colney Lane, NR4 7UH, Norwich, UK [email protected]

Professor Peter Shaw Department of Cell and Developmental Biology John Innes Centre Colney Lane, NR4 7UH, Norwich, UK [email protected]

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Professor David Spector Director of Research Cold Spring Harbor Laboratory One Bungtown Road Cold Spring Harbor, NY 11724 [email protected]

Dr. Luis Felipe Jimenez García Departamento de Biología Celular Facultad de Ciencias/UNAM Av. Universidad 3000, Circuito Exterior S/N Delegación Coyoacán, C.P. 04510. Ciudad Universitaria, Mexico City. [email protected]

Dra. Elena Alvarez-Buylla Roces Departamento de Ecologia Funcional Instituto de Ecologia/UNAM Circuito exterior s/n anexo al Jardín Botánico Exterior UNAM, 04510 Ciudad Universitaria, Mexico City. [email protected] [email protected]

Dra. Alejandra Alicia Covarrubias Robles Departamento de Biologia Molecular de Plantas Instituto de Biotecnologia/UNAM Av. Universidad #2001, Col. Chamilpa C.P. 62210 Cuernavaca, Morelos Apdo. Postal 510-3, C.P.62250 [email protected]

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