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Supplemental Data Supplemental Data Genomic analyses of the extinct Sardinian dhole (Cynotherium sardous) reveal its evolutionary history Authors: Marta Maria Ciucani1, Julie Kragmose Jensen2, Mikkel-Holger S. Sinding3, Oliver Smith1,4, Saverio Bartolini Lucenti5,6, Erika Rosengren7, Lorenzo Rook5, Caterinella Tuveri8, Marisa Arca8, Enrico Cappellini1, Marco Galaverni9, Ettore Randi10, Chunxue Guojie11, Guojie Zhang11,12,13,14, Thomas Sicheritz-Pontén1,15, Love Dalén16,17, M. Thomas P. Gilbert1,18, Shyam Gopalakrishnan1,2 Corresponding author: [email protected] Authors affiliation: 1 Section for Evolutionary Genomics, GLOBE Institute, University of Copenhagen, Copenhagen, Denmark 2 Department of Health Technology, Technical University of Denmark, Kongens Lyngby, Denmark 3 Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland 4 Micropathology Ltd, University of Warwick Science Park, Coventry, UK 5 Dipartimento di Scienze della Terra, Paleo[Fab]Lab, Università di Firenze, Via G. La Pira 4, 50121, Firenze, Italy 6 Sezione di Geologia e Paleontologia, Museo di Storia Naturale, Università degli Studi di Firenze, Via G. La Pira 4, 50121, Firenze, Italy 7 Department of Archaeology and Ancient History, Lund University, Helgonavägen 3, Box 192, 221 00 Lund, Sweden 8 Soprintendenza Archeologia, Belle Arti e Paesaggio per le province di Sassari e Nuoro (Ufficio Operativo di Nuoro), Via G. Asproni 8, 08100, Nuoro, Italy 9 WWF Italy, Science Unit, Rome, Italy 10Department of Chemistry and Bioscience, Faculty of Engineering and Science, University of Aalborg, Aalborg, Denmark 11 BGI-Shenzhen, Shenzhen, 518083, China 12 Villum Center for Biodiversity Genomics, Section for Ecology and Evolution, Department of Biology, University of Copenhagen, Denmark 13 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China 14 Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming 650223, China 15 Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio), Faculty of Applied Sciences, AIMST University, Batu 3 1/2, Butik Air Nasi, 08100 Bedong, Kedah, Malaysia 16 Centre for Palaeogenetics, Svante Arrhenius väg 20C, 10691 Stockholm, Sweden 17 Department of Bioinformatics and Genetics, Swedish Museum of Natural History, Box 50007, 10405 Stockholm, Sweden. 18 Norwegian University of Science and Technology, University Museum, Trondheim, Norway Figure S1. Nucleotide misincorporation rates, length distribution and chromosome coverage of the Sardinian dhole. A) Nucleotide misincorporation rates of the sample mapped to the wolf reference genome. B) Length distribution of the total reads mapped to the wolf reference genome. C) Nucleotide misincorporation rates of the sample mapped to the CanFam3.1 reference genome. D) Length distribution of the total reads mapped to the CanFam3.1 reference genome. E) Biological sex determination. The sequencing reads sample were mapped to the dog reference genome (CanFam3.1)1, which present the genome divided in 38 autosomal chromosomes and the sex chromosome X. The plot here shows on the x axis the average depth of coverage at each chromosome of the Sardinian dhole which resulted to be ~ 5x across the whole genome. However, the average depth of coverage for the chromosome X was higher (> 6x) than the average depth of each chromosome and the whole genome, thus we concluded that our sample was female. Figure S2. PCA, Admixture proportions, and Astral tree A) Principal component analysis (PCA) of 41 canids excluding Andean fox and the African hunting dogs B) The admixture proportions were estimated using NGSAdmix2, for a range of ancestry components from K=2 to K=15. C) The phylogeny of 47 samples was computed by Astral III3 on 1000 gene trees generated with RAxML-ng4 under the evolutionary model GTR-GAMMA. Dogs, Eurasian, North-American and Chinese highland wolf nodes were collapsed separately. The node labels represent the posterior probability computed by RAxML-ng using 100 replicated in Astral-III. Branch nodes with a posterior probability above 0.75 are shown. Figure S3. D-stats In this figure the gene flow among different canids is shown using ABBA-BABA test in ANGSD5. The following three different combinations were tested: 1) (((Canis lupus, Golden Jackal), Sardinian dhole), Andean fox), 2) (((Canis lupus, African golden wolf), Sardinian dhole), Andean fox) and 3) (((Canis lupus, Ethiopian wolf), Sardinian dhole), Andean fox). In certain places in the figure the species names are abbreviated with AF, SD, GJ, AGW and EW for Andean for, Sardinian dhole, Golden jackal, African golden wolf and Ethiopian wolf respectively. In the figure A-B) the first combination is shown and there is significant allele sharing (D < 0) between the coyotes, wolves and dogs to/from Sardinian dhole (SD) when considering two Golden jackals (GJ) (Syria and Israel golden jackals) in H2. In panel C-D) the combination 2) was tested and the allele sharing between the AGW and SD when placing the GJ in H1. However, when considering Canis lupus in H1 the D-stats shift to a negative result (D < 0) indicating gene flow into the Sardinian dhole. In figure E) the combination 3) is shown and when considering all the Canis crown species but Golden jackal (GJ) the D-statistics show a negative D (D < 0 - BABA) meaning that there is allele sharing between these species and the Sardinian dhole (SD). Graph 1 Graph 2 Graph 3 Graph 4 And WDF Bei Sar 0.000000 -0.008135 -0.008135 0.000532 -15.292 And WDF Bei Sar -0.008797 -0.008135 0.000661 0.000532 1.243 And WDF Bei Sar -0.008252 -0.008135 0.000117 0.000532 0.220 Bei WDF Sar Eth 0.000000 0.027180 0.027180 0.000853 31.852 R R R R 275 275 274 274 295 295 277 277 Q AndeanFox Q AndeanFox Q AndeanFox Q AndeanFox 13 170 237 46 36 80 aAHD BD 381 56 aAHD BD AHD BD 186 42 110 87 176 0 WDF20 BD 350 575 201 AHD 65% B SardinianDhole AHD 3% B BDC 314 221 WDF20 25% BDhole SardinianDhole 186 35% 87 97% 252 307 75% WDF20 BDhole BeijingZooDhole SardinianDhole WDF20 BDhole SardinianDhole EthiopianWolfB3 342 BeijingZooDhole 185 BeijingZooDhole BeijingZooDhole Graph 5 Graph 6 Graph 7 Graph 8 And WDF Bei Sar -0.009220 -0.008864 0.000356 0.000688 0.517 And Afr Sar Eth 0.000000 -0.003653 -0.003653 0.000463 -7.895 And Afr Eur Eth -0.011334 -0.011031 0.000303 0.000509 0.596 And Afr Eur Eth -0.011414 -0.011031 0.000383 0.000509 0.752 R R R R 295 295 296 296 295 295 295 295 Q AndeanFox Q AndeanFox AndeanFox Q AndeanFox Q 98 40 85 46 95 49 75 51 aAHD BD aAHD BD aAHD BD aAHD BD 157 30 157 177 3 32 159 175 3 33 158 183 3 37 158 AHD 9% B BDC AHD D BDC AHD D BDC AHD aD D BDC 157 91% 229 157 177 aD 166 117 159 171 1% aD 99% 166 158 183 5% 162 95% 158 WDF20 BDhole SardinianDhole EthiopianWolfB3 AfricanHuntingDog 11% E B EthiopianWolfB3 AfricanHuntingDog B 12% E EthiopianWolfB3 AfricanHuntingDog 15% E B EthiopianWolfB3 339 89% 117 233 88% 85% 234 BeijingZooDhole BDhole SardinianDhole SardinianDhole BDhole BDhole SardinianDhole 211 214 226 EurasianDhole EurasianDhole EurasianDhole Graph 9 Graph 10 Graph 11 Graph 12 And Afr Eur Sar -0.008405 -0.007378 0.001027 0.000474 2.166 And Afr Eur Sar -0.005639 -0.005040 0.000599 0.000752 0.796 And Afr Eur Eth -0.013058 -0.012830 0.000228 0.000848 0.269 Sar Afr Eth EuW -0.000000 -0.002482 -0.002482 0.000578 -4.298 R R R R 295 295 320 320 320 320 334 334 Q AndeanFox Q AndeanFox Q AndeanFox Q AndeanFox 6 105 13 153 28 86 30 92 aAHD BD aAHD BD aAHD BD aAH D BD 74 126 89 130 17 194 90 117 230 42 114 151 240 45 119 92 AHD aD A BDC AHD aD A BDC AHD aD A BDC AH D aD A BDC 361 54% 46% 130 457 60% 40% 117 230 28% 72% 151 240 28% 72% 222 170 AfricanHuntingDog D EthiopianWolfB3 AfricanHuntingDog D EthiopianWolfB3 AfricanHuntingDog D EthiopianWolfB3 AfricanH untingDog D EthiopianWolfB3 EuWolf 11% 167 117 25% 157 144 25% 293 155 E B 25% 306 162 E B SardinianDhole E 89% 117 SardinianDhole E 75% 144 75% BDhole SardinianDhole 75% BDhole SardinianDhole BDhole 210 BDhole 280 276 EurasianDhole 288 EurasianDhole EurasianDhole E urasianDhole Graph 13 Graph 14 Graph 15 Sar Afr Eth EuW 0.000000 -0.002482 -0.002482 0.000578 -4.298 And Afr E ur Sar -0.006392 -0.005304 0.001088 0.000780 1.395 And Afr E th E uW -0.001088 -0.000432 0.000657 0.000492 1.335 R R R 334 334 334 334 334 334 AndeanFox Q Q AndeanFox AndeanFox Q 31 95 14 149 24 104 aAH D BD aAH D BD aAH D BD 240 22 113 96 19 196 122 34 10 36 162 88 AH D aC BDC AH D aD A BDC AH D aD A BDC 240 45 4% A 96% 163 476 35% 4% 65% 96% 162 477 56% 44% 222 170 AfricanH untingDog aD 71% C E uWolf AfricanH untingDog D C E uWolf 29% 228 AfricanH untingDog D EthiopianWolfB3 EuWolf 25% 164 302 229 D E thiopianWolfB3 25% 164 302 25% 309 161 E SardinianDhole E thiopianWolfB3 E SardinianDhole SardinianDhole E 75% 75% 75% BDhole BDhole BDhole 292 292 286 E urasianDhole E urasianDhole EurasianDhole Figure S4. Admixture graph population history models. The demographic models of the Sardinian dhole, modern African hunting dogs, Eurasian dholes, Ethiopian wolves and Eurasian wolves, estimated using qpGraph of Admixtools6 with the observed and expected f-statistics to compute the admixture graph.
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