FOCUS The Effective SOD for Health & Skin

roduction of re- Thanks to its gliadin matrix, Vouldoukis et al. have First orally effective SOD, active oxygen GliSODin® is orally effective shown ex vivo that species (ROS) is unlike most existing SOD prime activation of mac- GliSODin® is the new oral a normal process supplements. rophages isolated from Pin oxygen-breathing or- rodents with interferon- active for skin health, ganisms. Under normal The gliadin is not only an enteric gamma (INF-gamma) physiological conditions protection but more : a real subsequently challenged immune system and a balance between these vehicle for the SOD, thanks to its with IgGl/anti-IgG1 im- species and the body’s specific bio-adhesive properties mune complexes leads free radicals protection sup- anti-oxidant defenses with the epithelial cells in the to the significant pro- ported by more than exists. However, certain small intestine. duction of superoxide conditions can increase anions. This production 25 mostly published studies the production of may be regulated, in a dose-dependent man- (ROS) such as the superoxide ion (O2-) and ner, in macrophages originating from rodents the hydroxyl ion (OH-).This can disrupt the previously supplemented with GliSODin®. • GliSODin® is both a protected and targeted SOD, natural balance and lead ultimately to oxida- These results prove the potent in vivo acti- supported by more than 25 clinical and scientific tive stress. vation of activities made by the studies. SOD-containing melon extract/gliadin bio- • GliSODin®, a patented nutraceutical ingredient, Cells defend themselves? polymer combination. has been shown in clinical research to : An important proof of concept in vivo study (SOD), along with cat- • Promote the production of the body’s own, natu- by Vouldoukis et al. using Balb/c mice receiv- alase and glutathione peroxidase, form the ral ing the SOD melon extract orally, either alone front line of the body’s antioxidant enzyme • Help maintain cellular health and protect against or combined with wheat gliadin (GliSODin®) defenses[4].The superoxide anion is the start- damage caused by for 28 weeks showed that only the gliadin- ing point of the cascade of reactions of free • Inhibition of endometriosis extension (mice) SOD complex resulted in a significant in- radical production. • Support skin health against photo-oxidative stress crease in circulating antioxidant levels. SOD, dubbed the “enzyme of life” on discov- • Reduce lactic acid buildup under physical stress At the end of the study, the animals supple- ery in 1968, is the first antioxidant mobilized • Support healthy immune function mented with GliSODin® had significantly by the cell as defense against oxidative stress. • Promote cardiovascular health lower levels of oxidative-stress induced DNA The enzyme reacts with the superoxide ion • Prevent stress induced impairment of spatial damage. Furthermore, the researchers found and turns it into hydrogen peroxide (H2O2). memory (neuroprotection) lower levels of apoptotic cells in the spinal This is then cataboliwzed by catalase and fluid, thus showing a marked protective ben- glutathione peroxidase to produce molecular efit. oxygen (O2) and water (H2O). These antioxidant enzymes have a distinct advantage over the antioxidants consumed Oxidative stress & from the diet or nutritional supplements, DNA damages like the vitamins A, C, and E, carotenoids, GliSODin®’s protective benefits have been and thiols since the enzymes are biological demonstrated in human interventional stud- catalysts, rapidly and repeatedly reducing ies. GliSODin® was shown to protect against reactive oxygen species without being con- cellular oxidative stress damage in a dramatic sumed themselves. Like most other protec- human model. In this double-blind, placebo- tive mechanisms in the body, the production controlled trial, twenty healthy volunteers of SOD decreases with age[5], while a cell’s were given pure oxygen in a hyperbaric Anti-aging susceptibility to oxidants increases, putting chamber (HBO), which increased atmospher- • Protects against DNA damage the cells under increasing oxidative stress. ic pressure to 2.5 times normal, inducing • Prevents oxidative stress intense oxidative stress. A measure of blood • Decreases troubles induced by ageing Oral administration cell protection, looking at the integrity of • Improves cognitive functions Oral administration of SOD and the other the cellular DNA, was taken before and after • Protects against photo aging antioxidant enzymes present in many plant exposure to measure the effect of oxidative • Supports healthy immune function extracts is, under normal conditions, not ef- stress. The GliSODin® group had significantly • Reduces risk factors of cardiovascular disease fective. During passage through the gastro- lower cellular DNA damage as evidenced by a • Helps prevent light sun allergies intestinal pathway the enzyme is denatured test called “Comet Assay.” Also, these findings (deactivated) rendering it ineffective as an coincided with reduced blood isoprostane antioxidant. However, studies have shown levels, another marker of oxidative stress. that combining SOD with a wheat gliadin In the GliSODin group, the cell nucleus is biopolymer system temporarily protects the intact, while the placebo group shows pro-

FOCUS HINTS SOD during its passage through the gastro- gressive nucleus damage with pronounced intestinal tract. One explanation of this ef- “comet tail”. ficiency was presented by Clemente et al. www.glisodin.org & www.glisodin.org who showed that gliadin increases the per- meability of the intestinal wall by promoting The enzymatic activity of oral free SOD the release of a zonulin, thereby allowing the in the body is mainly to totally de- transport of the macromolecule SOD across stroyed within few minutes, despite the the intestinal barrier. source, and can so not promote the pro- The combination of SOD extracted from duction of the SOD cascade by the cells cantaloupe melon (Cucumis melo L.C.) with and the related expected optimal activi- the wheat gliadin biopolymer (GliSODin®) ties. For this reason, usual studies based significantly improves the delayed release of on free SOD cannot prove the efficacy, SOD as evidenced in vitro by the progressive or at an incredibly high use (in-vivo). increase of its activity in a medium mimicking Dose : 2 x 100mg to 2 x 250mg a day the digestive conditions .

28 - BECARRE Natural - October 2020 sis, which is a major risk factor for cardiovascular dis- ease. Sun & skin health GliSODin® promoted arterial health and function com- • Increases the MED (Minimal Erythema Dose) pared to the control group as measured by the thick- • Helps prevent light sun allergies ness of the participants’ carotid arteries. Further, GliSO- • Prepares the skin for sun exposure Din® significantly improved the antioxidant status and provided a reduction in measures of lipid oxidation. The • Reduces sunburn redness researchers called the results «remarkable”. • Faster recovery after sunburn and photo- (Naito et al.) No significant differences in blood glu- oxidative stress cose levels or body weight were observed between the diabetic mice in the SOD-gliadin- supplemented group and the control group. In terms of kidney health, signifi- cant reductions in the levels of 8- hydroxydeoxyguano- sin (8-OHdG), a marker of oxidative stress, had been observed in the SOD-gliadin-supplemented group.

Sport & inflammation GliSODin® was studied for its ability to regulate the • Anti-inflammatory effect: control C-Reactive immune response. Supplementation with GliSODin® Protein had increased production of type1 helper T lympho- • Decreases in-exercise lactate production cytes (Th1) and INF-a and IL-4.The immunoglobulin • Supports post-exercise immune function G (IgG) response – the predominant antibody used by • Improves recovery after strenuous exercise the body to identify and neutralize foreign objects, while the response of IgE, the immunoglobulin associated with an allergic response was only marginally affected. Vouldoukis et al. proposed that the mechanism behind these effects was due to an activation of antigen pre- senting cells (APC), which results in the production of A randomized double-blind clinical trial by Mac-Mary et hydrogen peroxide (H2O2) and nitric oxide (NO), both al. showed that GliSODin® supplementation reduced of which are reactive oxygen species and upset the skin-reddening when healthy fair-skinned volunteers oxidant-antioxidant balance. In response to this, pro- were exposed to UV radiation. Fifty subjects were ran- duction of the antioxidant enzymes catalase and gluta- domly assigned to receive a daily dose of GliSODin® thione peroxidase is induced. This results in a polarized (500 mg) or placebo four weeks. Subjects were ex- adaptive immune system, highlighting the benefits of posed to UV radiation to induce sunburn on the inner- GliSODin® since this polarization is a sign of the natural forearms, and the susceptibility of the participants to equilibrium of antioxidants in the cells. sunburn (defined as the minimum erythematous dose Rahman et al. investigated the effect of receiving – MED) and a measure of the resulting redness (actinic GliSODin®-containing supplements on quality of life erythema). and performance of 23 AIDS patients on HAART (sig- nificant benefits for the millions of people infected with Supplementation with GliSODin® results in an increase the HIV-1 virus and receiving anti-retroviral therapy). in the minimum exposure to UV rays necessary to pro- duce skin burn for fair-skinned people (phenotype II), compared to placebo. The induced redness also de- In an in vivo study, Vouldoukis et al. compared the ef- creased quicker in the GliSODin®-supplemented group fects on production of pro- and anti-inflammatory cyto- over the four-week period. These results confirmed the kines with SOD, Gliadine, heat inactivated GliSODin® efficacy of the SOD-gliadin combination against the and GliSODin®. consequences of oxidative stress produced by expo- Cardiovascular health Only GliSODin® reduced the production of the pro-in- sure to UV radiation. In a later study of similar design • Reduces risk factors of cardiovascular disease flammatory cytokine, tumor necrosis factor-alpha (TNF- with Type II (fair-skinned) participates, GliSODin sig- • Decreases carotid Intima-Media thickness a) and promoted production of the anti-inflammatory nificantly increased the MED in two weeks’ time with a (IMT) cytokine interleukin-10 (IL-10), compared to the other dose of just 250mg. • Prevents metabolic syndromerelated diseases treatments. The anti-inflammatory effects of GliSODin® are signifi- cant since chronic inflammation is associated with the onset and progression of many chronic diseases. Okada et al. reported that administration of the gliadin- Immunity SOD complex could prevent cancer progression, pro- • Supports healthy immune function moted by inflammation. This result indicated that the • Immune-modulating properties shown to antioxidant and anti-inflammatory properties of GliSO- be effective in children Din® might have significant benefits for the prevention • Acts as immune-modulator of tumor development and progression. • Regulates pro- and anti inflammatory A double-blind study included 19 participants who cytokines production were participating in a training camp (2 capsules (total • Induction of Th-1 immune response 500 mg) of GliSODin®). SOD activity was significantly New published study with GliSODin®: in the present higher (p <0.01) in the supplemented group at all mea- randomized, placebo-controlled, double-blind clinical surement times, and post-exercise C-reactive protein trial, the clinical efficacy of GliSODin® in accelerat- was significantly lower in athletes receiving GliSODin®. ing symptom relief in children with allergic asthma and Supplementation with an extract rich in SOD activity house dust mite allergy (receiving house dust mite promoted antioxidant status and protected against in- immunotherapy) was demonstrated. In summary, Gli- creased inflammation in the serum of professional row- SODin® accelerates the symptom relief in asthmatic A published, three-year study with GliSODin® supple- ers. children allergic to house dust mite receiving house mentation showed significant benefits for cardiovascu- Hong et al. recruited 44 healthy individuals and as- dust mite immunotherapy. For the first time GliSODin’s lar health. Using ultrasound-B imaging to measure ca- signed them to receive a daily GliSODin® dose of 1500 immune-modulating properties were shown to be ef- rotid artery intima thickness (IMT), a sign of hardening of IU for four weeks. Healthy volunteers were then submit- fective in children! the arteries (atherosclerosis), the researchers found a ted to cycling or treadmill exercise but assigned into reduction in the progression of IMT in the SOD-supple- two distinctive groups: the severe exercise group (27 mented group, compared to the group with the pre- subjects) or the moderate exercise group (17 subjects). scribed diet and lifestyle. This study showed that sup- Only volunteers ranging from the severe exercise group plementation with GliSODin® could impact the showed a significant reduction in the exercise-induced antioxidant status and the inflammatory process. The lactate production, after the four-weeks of SOD supple- study also showed clear benefits against atherosclero- mentation.

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