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Redalyc.LECTURES Journal of Pharmacy & Pharmacognosy Research E-ISSN: 0719-4250 [email protected] Asociación de Académicos de Ciencias Farmacéuticas de Antofagasta Chile LECTURES (L) Journal of Pharmacy & Pharmacognosy Research, vol. 3, núm. 1, 2015, pp. sxlvi-s193 Asociación de Académicos de Ciencias Farmacéuticas de Antofagasta Antofagasta, Chile Available in: http://www.redalyc.org/articulo.oa?id=496050652001 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Topes de Collantes, Santi Spiritus, Cuba Cuban Society of Pharmacology Journal of Pharmacy & Pharmacognosy Research Volume 3, Supplement 1 (September) : S1 -S189 , 2015 INDEX http://jppres.com/jppres J Pharm Pharmacogn Res (2015) 3(Suppl. 1):s xlvi Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Topes de Collantes, Santi Spiritus, Cuba Cuban Society of Pharmacology Journal of Pharmacy & Pharmacognosy Research Volume 3, Supplement 1 (September) : S1 -S189 , 2015 LECTURES (L) L-01: ELUCIDATING POLYPHARMACOLOGICAL studies, it is possible to identify activated or repressed MECHANISMS OF CANCER CHEMOPREVENTION BY pathways and to predict possible molecular targets. MANGIFERIN AND WITHAFERIN A BY TRANSCRIPTOME Direct phytochemical-protein interactions can be AND (CHEMO) PROTEOME BASED PATHWAY AND DRUG verified by (chemo)proteomic approaches. The results TARGET ENRICHMENT ANALYSIS show that the medicinal effects of withaferin A and Hernández-Balmaseda I 1, Szarc vel Szic K 2, Chirumamilla CS 2, mangiferin extend far beyond well-known antioxidant Palagani A 2, Hassania B 3,4 , Declerck K 2, Dom M 2, Naulaerts S 5, activities of natural products. This method is also Op de Beeck K 6, Kaileh M 4,7 , Haegeman G 4, Van Camp G 6, applicable to dissect the polypharmacology of other Laukens K 5, Heyninck K 4, Van Ostade X 2, Vanden Berghe T 3, natural products or crude plant extracts. Vandenabeele P 3, Rodeiro-Guerra I 1, Delgado-Hernández R 8, Vanden Berghe W 2,4 . 1Laboratory of Pharmacology, Bioactive Marine Center, Havana, Cuba. 2Laboratory of Protein Chemistry, Proteomics and Epigenetic Signaling (PPES), Department of Biomedical Sciences, University of Antwerp (UA), Antwerp, Belgium. 3 Inflammation Research Center, VIB, Ghent University, Belgium. 4Laboratory of GPCR Expression & Signal Transduction (L-GEST), Ghent University, Belgium. 5Advanced Database Research and Modeling (ADReM), Biomina, University of Antwerp (UA), Belgium. 6Laboratory of Cancer Research and Clinical Oncology, Center of Medical Genetics, University of Antwerp, Belgium. 7Laboratory of Molecular Biology and Immunology, National Institute on Aging, National Institutes of Health, Baltimore, USA. 8Division of Research and Development, Center for Pharmaceutical Research and Drug Development (CIDEM), Havana, Cuba. Presenting author: [email protected] Drugs designed to act against individual molecular Citation Format: targets usually cannot combat multigenic diseases such Hernández-Balmaseda I, Szarc vel Szic K, Chirumamilla CS, Palagani as cancer, or diseases that affect multiple tissues or cell A, Hassania B, Declerck K, Dom M, Naulaerts S, Op de Beeck K, types such as diabetes and immunoinflammatory Kaileh M, Haegeman G, Van Camp G, Laukens K, Heyninck K, Van Ostade X, Vanden Berghe T, Vandenabeele P, Rodeiro-Guerra I, disorders. Combination drugs that affect multiple targets Delgado-Hernández R, Vanden Berghe W (2015) Elucidating simultaneously are better at controlling complex disease polypharmacological mechanisms of cancer chemoprevention by systems, are less prone to drug resistance and are the mangiferin and withaferin a by transcriptome and (chemo) proteome standard of care in many important therapeutic areas. based pathway and drug target enrichment analysis. Hepatotoxicity The identification of biologically active and potentially and oxidative stress biomarker study of peg-coated and non-coated gold nanoparticles in Sprague-Dawley rats. Abstract. In: Proceedings therapeutically useful pharmacophores from natural of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, products has been a long-term focus in the Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S 1 . Abstract pharmaceutical industry. The steroidal withanolide nr L-01. withaferin A ( Withania somnifera ) and the xanthone polyphenol mangiferin ( Mangifera indica ) hold much promise as potential novel cancer chemopreventive or therapeutic compounds. However, it is a great challenge to elucidate their polypharmacological anti-cancer mechanisms. To address this challenge, we apply bioinformatics methods to identify multiple targets of chemical agents through analysis of the gene expression profiles following in vitro /in vivo treatment. By using transcriptome based pathway and drug target enrichment analysis and pharmapper in silico modeling http://jppres.com/jppres J Pharm Pharmacogn Res (2015) 3(Suppl. 1):S 1 Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Topes de Collantes, Santi Spiritus, Cuba Cuban Society of Pharmacology Journal of Pharmacy & Pharmacognosy Research Volume 3, Supplement 1 (September) : S1 -S189 , 2015 L-02: PREDICTIVE STRATEGY TO SCREENING NATURAL CANDIDATES FOR TYPE 2 DIABETES BASED ON PTP-1B AND DPP-IV INHIBITORY ACTIVITY AND in itro GLUCOSE UPTAKE Marrero E 1, Sánchez J 1, Young L 2, Harvey A 2. 1Grupo de Desarrollo Biofarmacéutico/Centro Nacional de Sanidad Agropecuaria (CENSA), San José de las Lajas, Mayabeque, Cuba. [email protected] , [email protected] 2Strathclyde Institute for Biomedical Sciences/Strahclyde University, Scotland, United Kingdom. Introduction: The present study is aimed to determine Citation Format: the underlying mechanism of the antidiabetic Marrero E, Sánchez J, Young L, Harvey A (2015) Predictive strategy to potentiality of seven Cuban plants based on relevant screening natural candidates for type 2 diabetes based on PTP-1b and DPP-iv inhibitory activity and in itro glucose uptake. [Abstract]. In: therapeutic target of type 2 diabetes: Allophylus cominia Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de (L.) Sw., Ocimum tenuiflorum , Persea americana , Sechium Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): edule , Momordica charantia and Jatropha aethiopica . S2. Abstract nr L-02. Methods: Leaves aqueous extracts and its fractions were evaluated on protein tyrosine phosphatase 1B (PTP1B) and dipeptidyl peptidase-IV (DPPIV) enzymatic activities and the glucose uptake to identify candidates as phytopharmaceuticals in treating diabetes . In PTP1B assay (DiFMUP) was used as substrate and (TFMS) as standard inhibitor; in DPPIV assay (Gly-pro-AMC) was used as substrate and as standard inhibitor (P32/98); in both assays, the enzymatic activity inhibition was calculated by fluorescence using excitation wavelength of 360 nm and emission wavelength of 460 nm. Glucose uptake studies were performed on fully differentiated 3T3-L1 adipocyte using 2-deoxy-D-3H glucose and insulin as positive control, radioactivity into the cells was measured with a microplate scintillation counter. Results: The extracts from A. cominia , O. tenuiflorum and P. americana inhibited the enzymatic activity of PTP1B in a concentration dependent manner, resulting more actives the polar fractions from A. cominia and P americana. All the extracts and fractions showed inhibition of enzymatic activity of DPPIV, at higher concentrations. A. cominia inhibited enzymatic activity of this protease in an extract concentration dependent manner. A. cominia , O. tenuiflorum and P. americana extracts exhibited enhance of glucose uptake in 3T3-L1 adipocytes. Conclusions: The aqueous extracts from A. cominia , P. americana and O. tenuiflorum and some of its fractions could be promising antidiabetic candidates. Further A. cominia studies based on bio-guided estructure-activity assays of the aqueous extracts and fractions revealed quercitrin and mearnsetrin flavonoids as the key components in that activity. http://jppres.com/jppres J Pharm Pharmacogn Res (2015) 3(Suppl. 1):S 2 Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Topes de Collantes, Santi Spiritus, Cuba Cuban Society of Pharmacology Journal of Pharmacy & Pharmacognosy Research Volume 3, Supplement 1 (September) : S1 -S189 , 2015 L-03: NATURAL PRODUCTS: A KEY-POINT FOR THE CUBAN PROGRAM OF TRADITIONAL AND NATURAL MEDICINE Perdomo-Delgado J . Ministry of Public Health, Havana, Cuba. E-mail: [email protected] In 1991 Commander in Chief Fidel Castro indicated to Citation Format: create the Program of Medicinal Plants in order to Perdomo-Delgado J (2015) Natural products: a key-point for the promote its rational and scientific usage, as a part of the Cuban program of traditional and natural medicine. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de development foreseen for the Cuban Pharmaceutical Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): Industry. During the first half of the nineties’
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