The role of primary cilia and sonic hedgehog signalling in adrenal development function. Cogger, Kathryn The copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the author For additional information about this publication click this link. http://qmro.qmul.ac.uk/jspui/handle/123456789/3167 Information about this research object was correct at the time of download; we occasionally make corrections to records, please therefore check the published record when citing. For more information contact
[email protected] THE ROLE OF PRIMARY CILIA AND SONIC HEDGEHOG SIGNALLING IN ADRENAL DEVELOPMENT AND FUNCTION Kathryn Cogger A thesis submitted to Queen Mary, University of London for the degree of Doctor of Philosophy August 2012 Centre for Endocrinology William Harvey Research Institute Barts and The London School of Medicine and Dentistry Queen Mary University of London ABSTRACT ABSTRACT Primary cilia are sensory organelles found on most vertebrate cells during interphase. They play key roles in development, cell signalling and cancer, and are involved in signal transduction pathways such as Hh and Wnt signalling. The adrenal cortex produces steroid hormones essential for controlling homeostasis and mediating the stress response. Signalling pathways involved in the process of its development and differentiation are still being identified but include Hh and Wnt, and adrenal development is thus likely to require cilia. I have demonstrated that inhibiting cilia formation, using siRNA targeted to different ciliary components, results in reduced differentiation of the human adrenal carcinoma cell line H295R towards a zona glomerulosa (zG)-like phenotype.