Adding Phylogenies to QGIS and Lifemapper for Evolutionary Studies of Species Diversity Jeffery A

Total Page:16

File Type:pdf, Size:1020Kb

Adding Phylogenies to QGIS and Lifemapper for Evolutionary Studies of Species Diversity Jeffery A Free and Open Source Software for Geospatial (FOSS4G) Conference Proceedings Volume 14 Portland, Oregon, USA Article 4 2014 Adding Phylogenies to QGIS and Lifemapper for Evolutionary Studies of Species Diversity Jeffery A. Cavner University of Kansas (USA) Aimee M. Stewart Charles J. Grady James H. Beach Follow this and additional works at: https://scholarworks.umass.edu/foss4g Part of the Geography Commons Recommended Citation Cavner, Jeffery A.; Stewart, Aimee M.; Grady, Charles J.; and Beach, James H. (2014) "Adding Phylogenies to QGIS and Lifemapper for Evolutionary Studies of Species Diversity," Free and Open Source Software for Geospatial (FOSS4G) Conference Proceedings: Vol. 14 , Article 4. DOI: https://doi.org/10.7275/R5T72FN2 Available at: https://scholarworks.umass.edu/foss4g/vol14/iss1/4 This Paper is brought to you for free and open access by ScholarWorks@UMass Amherst. It has been accepted for inclusion in Free and Open Source Software for Geospatial (FOSS4G) Conference Proceedings by an authorized editor of ScholarWorks@UMass Amherst. For more information, please contact [email protected]. Adding Phylogenies to QGIS and Lifemapper Adding Phylogenies to QGIS and Lifemapper for Evolutionary Studies of Species Diversity regulation of species distributions, should assay the spatial variation of phylogenies by mapping phylo- by Jeffery A. Cavner, Aimee M. Stewart, Charles J. Grady, genetic community values across space and time at James H. Beach different scales using advances in GIS techniques. One such approach would to be bring phyloge- University of Kansas (USA). [email protected] netic data into a GIS environment. We have be- gun to develop such an approach as an addition to Abstract the Lifemapper project (www.lifemapper.org) in a Phylogenetic data from the “Tree of Life” have ex- Lifemapper Range & Diversity (LmRAD) QGIS plug- plicit spatial and temporal components when paired in (Cavner et al. 2014) that provides phylogenetic with species distribution and ecological data for test- visualization and analysis tools for spatially linked ing contributions to biological community assembly range-diversity relationships derived from presence- at different geographic scales of species interaction. absence matrices (PAMs). We developed the tool also Important questions in biology about the degree of hoping to expand it to include historical biogeogra- niche suitability and whether the history of a com- phy meta-community analyses and community as- munity’s assembly for an area can affect whether the sembly analyses focused on phylogenetic-diversity species in a community are more or less phyloge- area relationships where analysis across geographic netically related can be answered using several dif- scale leads some of the most important questions in ferent spatially-filtered measures of phylogenetic di- biodiversity. versity. Phylogenetic analyses which support the de- The LmRAD QGIS plug-in creates, maps and an- scription of ecological processes are usually achieved alyzes presence-absence matrices or PAMs, one of in a handful of software libraries that are narrowly the core data structures for macroecological research. focused on a single set of tasks. Very few applications It links the resulting data to phylogenetic and spa- scale to large datasets and most do not have an ex- tial views of a set of range-diversity statistics de- plicit spatial component without relying on external rived from the PAM. The PAM or incidence matrix visualization packages. This prompted us to explore is a 2-dimensional Boolean matrix constructed from bringing phylogenetic data into an open-source GIS a spatially defined grid of regular polygons where environment. The Lifemapper Macroecology/Range the presence or absence of each species of hundreds & Diversity QGIS plug-in is a custom plug-in which or thousands of species are recorded for each cell. we use to calculate and map biodiversity indices that One axes of the matrix represents species and the describe range-diversity relationships derived from orthogonal axis represents geographic localities de- large multi-species datasets. We describe extensions scribed by the regular polygons. Each geographic to that plug-in which expand the Lifemapper set site is coded for the presence (1) or absence (0) of of ecological tools to link phylogenies to spatially- each species. It summarizes the two fundamental derived ’diversity field’ statistics that describe the units of biogeography, the distributional range of a phylogenetic composition of natural communities. species (both their position and size, range size sim- Keywords: QGIS, WPS, Distributed Comput- ply equals the total of the species axes across sites) ing, Biogeography, Range and Diversity, Lifemapper, and the species diversity of sites or the number of Macroecology, Phylogenetics. different species in each site as summarized by site axes totals. Several mathematical and biological relation- 1. Background ships obtain across the PAM that link spatially de- rived statistics with species based statistics. Of in- Community phylogenetics, the focus on how species terest for phylogenetic relationships are the species relatedness and species traits are associated with based statistics calculated from the PAM that mea- how evolution extends into ecological processes sure the “diversity field” of a species (Arita et al. and spatial patterns, and biogeography or meta- 2008). The diversity field is the set of diversity values community ecology, largely focused on the spatial of sites in which a species occurs. For example, the OSGEO Journal Volume 14 Page 19 of 48 Adding Phylogenies to QGIS and Lifemapper diversity field volume, i.e. the summation of those as Open Geospatial Consortium (OGC) Web Process- species diversity values within a species’ range di- ing Services (WPS) (Open Geospatial Consortium, vided by the range size of the species allows us to cal- Inc. 2007b) so that larger distributed computing en- culate the average species diversity within the range vironments can be brought to bear on large datasets. of that species. We represent that volume as a pro- The Lifemapper web services are organized as two portion of the total number of species in the study modules, LmSDM, and LmRAD. The LmSDM mod- area. Including the total area of the study area allows ule uses RESTful and OGC specifications to build us to illustrate the proportion of the sites in which species distribution models based on the predicted two species co-occur. The average association of a niche for a species using climate and species occur- species with all of the species in the study area al- rence data. The LmRAD (Range and Diversity) is a lows us to illustrate that there is an inverse relation- multi-species platform for PAM based range and di- ship between the proportional range of a species and versity calculations. Both modules can be accessed the difference between the mean proportional diver- through the plug-in, and outputs from LmSDM can sity within its range and the average proportional be piped into LmRAD as species inputs to PAMs. diversity in the study area (Arita et al. 2008). The This paper will focus on the range and diversity ca- mathematical reciprocal of the average proportional pabilities of the plug-in and how the spatial compo- diversity of the study area is a well-studied measure nent to phylogenetic data recently added to the plug- of species turnover called Whittaker’s beta diversity. in can be used with the biodiversity indices calcu- It is a measure of the ratio between the overall di- lated from the PAM and areas where phylogenetic versity of the study area and the average local di- data can be used to explore other types of diversity versity (Arita et al. 2008). There are closely associ- measures for species communities. This paper will ated beta measures of diversity for several different begin by outlining use cases and common threads types of diversity. Different approaches to species di- that connect them and how we have begun to ad- versity such as phylogenetic diversity – the degree dress them with a focus on new interface capabilities of relatedness of species in a community based on for phylogenetic data and linked data spaces. Next their evolutionary history – abundance and ecosys- we will describe how the Lifemapper plug-in and tem function measures of diversity all can be decom- it’s supporting web services were designed to take posed into measures of local and regional diversity advantage of a client-server architecture in order to ratios that are highly dependent on scale. be able to use geographic processing standards on Analyzing the diversity field within the range of a large datasets. This is followed by a comparison of species is equivalent to studying it’s covariance with related software with a focus on phylogenetic algo- all the species in a study, i.e. the degree of associa- rithms and scripts with a spatial component. We end tion of species within their ranges. We plot this as- by discussing findings, and future directions for the sociation in QGIS through the plug-in in a “range- Lifemapper plug-in. diversity” plot. Curves on the plot for species follow a line defined by the inverse relationship between the range of a species and the difference between the two 2. Use Cases and Capabilities diversity statistics. When plotting the species in this way, species with equal degrees of association with 2.1 Range and Diversity Plots and Maps one another arrange themselves along lines of isoco- with Phylogenetic Trees variance. The Lifemapper plug-in allows the user to “brush” data points along those curves in the interac- Phylogenetic based ecology is a growing field. Its tive range-diversity plot which selects the individual practice both at small scales and larger biogeo- species in the linked data space for the phylogenetic graphic scales – it goes under several names: phylo- tree. In this way the spatially derived statistics for di- geography, ecophylogenetics, or phylogenetic com- versity from the PAM can be compared to the degree munity ecology – share two obvious constraints of phylogenetic relatedness within species commu- for incorporating phylogenetic data into ecology re- nities.
Recommended publications
  • Ade4: Analysis of Ecological Data: Exploratory and Euclidean Methods
    Package ‘ade4’ September 16, 2021 Version 1.7-18 Title Analysis of Ecological Data: Exploratory and Euclidean Methods in Environmental Sciences Author Stéphane Dray <[email protected]>, Anne-Béatrice Du- four <[email protected]>, and Jean Thioulouse <[email protected]>, with con- tributions from Thibaut Jombart, Sandrine Pavoine, Jean R. Lobry, Sébastien Ollier, Daniel Bor- card, Pierre Legendre, Stéphanie Bougeard and Aurélie Siberchicot. Based on ear- lier work by Daniel Chessel. Maintainer Aurélie Siberchicot <[email protected]> Depends R (>= 2.10) Imports graphics, grDevices, methods, stats, utils, MASS, pixmap, sp Suggests ade4TkGUI, adegraphics, adephylo, ape, CircStats, deldir, lattice, spdep, splancs, waveslim, progress, foreach, parallel, doParallel, iterators Description Tools for multivariate data analysis. Several methods are provided for the analy- sis (i.e., ordination) of one-table (e.g., principal component analysis, correspondence analy- sis), two-table (e.g., coinertia analysis, redundancy analysis), three-table (e.g., RLQ analy- sis) and K-table (e.g., STATIS, multiple coinertia analysis). The philosophy of the package is de- scribed in Dray and Dufour (2007) <doi:10.18637/jss.v022.i04>. License GPL (>= 2) URL http://pbil.univ-lyon1.fr/ADE-4/ BugReports https://github.com/sdray/ade4/issues Encoding UTF-8 NeedsCompilation yes Repository CRAN Date/Publication 2021-09-16 11:30:02 UTC R topics documented: ade4-package . .8 1 2 R topics documented: abouheif.eg . .8 acacia . .9 add.scatter . 10 aminoacyl . 13 amova............................................ 14 apis108 . 15 apqe............................................. 16 aravo............................................. 17 ardeche . 18 area.plot . 19 arrival............................................ 21 as.taxo . 22 atlas .
    [Show full text]
  • "Introduction to Inferring Evolutionary Relationships". In: Current
    Introduction to Inferring Evolutionary UNIT 6.1 Relationships Much of bioinformatics is essentially comparative biology. Inferences based on compari- sons between entities such as motifs, sequences, genomes, and molecular structures are made. Given that living organisms and their constituent components have an evolutionary history, phylogeny properly lies at the heart of comparative biology (Harvey and Pagel, 1991). Increasingly, researchers in bioinformatics are realizing that phylogeny-based comparisons can yield important insights that can be missed using other techniques (Eisen, 1998; Rehmsmeier and Vingron, 2001). For example, a knowledge of phylogeny is vital in determining whether a set of sequences are orthologous or paralogous (Page and Charleston, 1997; Yuan et al., 1998; Storm and Sonnhammer, 2001; Zmasek and Eddy, 2001), which in turn has implications for predicting the function of a novel sequence (Eisen, 1998). Indeed, it is increasingly common for protein family databases to include gene phylogenies in addition to alignments. Examples include HOVERGEN (http://pbil.univ-lyon1.fr/databases/hovergen.html; Duret et al., 1994), SYSTERS (http://systers.molgen.mpg.de; Krause et al., 2000), and COPSE (http://copse.molgen. mpg.de). Phylogenetic analysis at the level of whole genomes poses new analytical challenges (Kim and Salisbury, 2001; Korbel et al., 2002; Wang et al., 2002), but promises new insights into genomic evolution. In some cases, the role of phylogeny might not be either obvious or explicit, so that many people may well have built phylogenetic trees without necessarily realizing it. The popular multiple sequence alignment program Clustal (UNIT 2.3) builds a phylogeny (the “guide tree”) every time it aligns sequences.
    [Show full text]
  • Romanesco Documentation Release 0.1.0
    Romanesco Documentation Release 0.1.0 Kitware, Inc. January 27, 2016 Contents 1 What is Romanesco? 1 1.1 Installation................................................1 1.2 Configuration...............................................1 1.3 Types and formats............................................2 1.4 API documentation............................................5 1.5 Developer documentation........................................ 11 1.6 Plugins.................................................. 13 2 Indices and tables 17 Python Module Index 19 i ii CHAPTER 1 What is Romanesco? Romanesco is a python application for generic task execution. It can be run within a celery worker to provide a distributed batch job execution platform. The application can run tasks in a variety of languages and environments, including python, R, spark, and docker, all via a single python or celery broker interface. Tasks can be chained together into workflows, and these workflows can actually span multiple languages and environments seamlessly. Data flowing between tasks can be automatically converted into a format understandable in the target environment. For example, a python object from a python task can be automatically converted into an R object for an R task at the next stage of a pipeline. Romanesco defines a specification that prescribes a loose coupling between a task and its runtime inputs and outputs. That specification is described in the API documentation section. This specification is language-independent and instances of the spec are best represented by a hierarchical data format such as JSON or YAML, or an equivalent serializable type such as a dict in python. Romanesco is designed to be easily extended to new languages and environments, or to support new data types and formats, or modes of data transfer.
    [Show full text]
  • Tutorial: Environment for Tree Exploration Release 3.0.0B7
    Tutorial: Environment for Tree Exploration Release 3.0.0b7 Jaime Huerta-Cepas November 25, 2015 Contents 1 Changelog history3 1.1 What’s new in ETE 2.3...................................3 1.2 What’s new in ETE 2.2...................................5 1.3 What’s new in ETE 2.1...................................9 2 The ETE tutorial 13 2.1 Working With Tree Data Structures............................ 13 2.2 The Programmable Tree Drawing Engine......................... 43 2.3 Phylogenetic Trees..................................... 64 2.4 Clustering Trees...................................... 80 2.5 Phylogenetic XML standards............................... 85 2.6 Interactive web tree visualization............................. 93 2.7 Testing Evolutionary Hypothesis.............................. 95 2.8 Dealing with the NCBI Taxonomy database........................ 105 2.9 SCRIPTS: orthoXML................................... 108 3 ETE’s Reference Guide 115 3.1 Master Tree class...................................... 115 3.2 Treeview module...................................... 127 3.3 PhyloTree class....................................... 138 3.4 Clustering module..................................... 141 3.5 Nexml module....................................... 142 3.6 Phyloxml Module..................................... 158 3.7 Seqgroup class....................................... 161 3.8 WebTreeApplication object................................ 161 3.9 EvolTree class....................................... 162 3.10 NCBITaxa class.....................................
    [Show full text]
  • User Manual for Splitstree4 V4.6
    User Manual for SplitsTree4 V4.6 Daniel H. Huson and David Bryant August 4, 2006 Contents Contents 1 1 Introduction 4 2 Getting Started 5 3 Obtaining and Installing the Program 5 4 Program Overview 6 5 Splits, Trees and Networks 7 6 Opening, Reading and Writing Files 10 7 Estimating Distances 10 1 8 Building and Processing Trees 11 9 Building and Drawing Networks 11 10 Main Window 12 10.1 Network Tab ........................................ 13 10.2 Data Tab .......................................... 13 10.3 Source Tab ......................................... 13 10.4 Tool bar ........................................... 13 11 Graphical Interaction with the Network 13 12 Main Menus 14 12.1 File Menu .......................................... 14 12.2 Edit Menu .......................................... 16 12.3 View Menu ......................................... 16 12.4 Data Menu ......................................... 17 12.5 Distances Menu ....................................... 19 12.6 Trees Menu ......................................... 19 12.7 Network Menu ....................................... 20 12.8 Analysis Menu ....................................... 21 12.9 Draw Menu ......................................... 21 12.10Window Menu ....................................... 22 12.11Configuring Methods .................................... 23 13 Popup Menus 23 14 Tool Bar 24 15 Pipeline Window 24 15.1 Taxa Tab .......................................... 25 15.2 Unaligned Tab ....................................... 25 15.3 Characters Tab
    [Show full text]
  • Load-Balance and Fault-Tolerance for Massively Parallel Phylogenetic Inference
    Load-Balance and Fault-Tolerance for Massively Parallel Phylogenetic Inference Master’s Thesis of Klaus Lukas Hübner at the Department of Informatics Institute of Theoretical Informatics, Algorithmics II Reviewer: Prof. Dr. Alexandros Stamatakis Second reviewer: Prof. Dr. Peter Sanders Advisor: Dr. Alexey Kozlov Second advisor: M.Sc. Demian Hespe 01 January 2020 – 30 June 2020 Abstract Upcoming exascale supercomputers will comprise hundreds of thousands of CPUs. Scientic applications on these supercomputers will face two major challenges: Hardware failures, and parallelization eciency. We extend RAxML-ng, a widely used tool to build phylogenetic trees, to mitigate hardware failures without user intervention. For this, we increase the checkpointing frequency. We also detect failures, redistribute the work among the surviving ranks, restore a consistent search state, and restart the tree search automatically. RAxML-ng now supports fault tolerance in the tree search mode, using multiple starting trees, and multiple alignment data partitions. RAxML-ng can handle multiple failures at once as well as multiple successive failures. There is no limit on the number of failures that can occur simultaneously or sequentially. We also support mitigating failures which occur during the recovery of a previous failure or during checkpointing. In contrast to the previously available manual recovery scheme, a recovery is initiated automatically after a failure, that is, the user does not have to take any action. We benchmark our algorithms for checkpointing and recovery. In our experiments, creating a checkpoint of the model parameters requires at most 72.0 ± 0.9 ms (400 ranks, 4,116 partitions). Creating a checkpoint of the tree topology requires at most 0.575 ± 0.006 ms (1,879 taxa).
    [Show full text]
  • Efficient Coalescent Simulation and Genealogical Analysis for Large Sample Sizes
    RESEARCH ARTICLE Efficient Coalescent Simulation and Genealogical Analysis for Large Sample Sizes Jerome Kelleher1*, Alison M Etheridge2, Gilean McVean1,2,3 1 Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom, 2 Department of Statistics, University of Oxford, Oxford, United Kingdom, 3 Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom * [email protected] Abstract A central challenge in the analysis of genetic variation is to provide realistic genome simula- tion across millions of samples. Present day coalescent simulations do not scale well, or use approximations that fail to capture important long-range linkage properties. Analysing the results of simulations also presents a substantial challenge, as current methods to store OPEN ACCESS genealogies consume a great deal of space, are slow to parse and do not take advantage of Citation: Kelleher J, Etheridge AM, McVean G shared structure in correlated trees. We solve these problems by introducing sparse trees (2016) Efficient Coalescent Simulation and Genealogical Analysis for Large Sample Sizes. PLoS and coalescence records as the key units of genealogical analysis. Using these tools, exact Comput Biol 12(5): e1004842. doi:10.1371/journal. simulation of the coalescent with recombination for chromosome-sized regions over hun- pcbi.1004842 dreds of thousands of samples is possible, and substantially faster than present-day Editor: Yun S. Song, UC Berkeley, UNITED STATES approximate methods. We can also analyse the results orders of magnitude more quickly Received: December 10, 2015 than with existing methods. Accepted: March 2, 2016 Published: May 4, 2016 Copyright: © 2016 Kelleher et al.
    [Show full text]
  • Tutorial: Environment for Tree Exploration Release 2.3.6
    Tutorial: Environment for Tree Exploration Release 2.3.6 Jaime Huerta-Cepas August 11, 2015 Contents 1 Changelog history3 1.1 What’s new in ETE 2.3...................................3 1.2 What’s new in ETE 2.2...................................5 1.3 What’s new in ETE 2.1...................................9 2 The ETE tutorial 13 2.1 Working With Tree Data Structures............................ 13 2.2 The Programmable Tree Drawing Engine......................... 43 2.3 Phylogenetic Trees..................................... 63 2.4 Clustering Trees...................................... 80 2.5 Phylogenetic XML standards............................... 85 2.6 Interactive web tree visualization............................. 92 2.7 Testing Evolutionary Hypothesis.............................. 94 2.8 Dealing with the NCBI Taxonomy database........................ 104 2.9 SCRIPTS: orthoXML................................... 108 3 ETE’s Reference Guide 115 3.1 Master Tree class...................................... 115 3.2 Treeview module...................................... 127 3.3 PhyloTree class....................................... 137 3.4 Clustering module..................................... 140 3.5 Nexml module....................................... 142 3.6 Phyloxml Module..................................... 157 3.7 Seqgroup class....................................... 160 3.8 WebTreeApplication object................................ 161 3.9 EvolTree class....................................... 161 3.10 NCBITaxa class.....................................
    [Show full text]
  • Rutger Aldo Vos Doctorandus, Universiteit Van Amsterdam, 2000 THESIS SUBMITTED in PARTIAL FULFILLMENT of the REQUIREMENTS for TH
    Rutger Aldo Vos Doctorandus, Universiteit van Amsterdam, 2000 THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in the Department of Biological Sciences 0Rutger Aldo Vos, 2006 SIMON FRASER UNIVERSITY Summer, 2006 All rights reserved. This work may not be reproduced in whole or in part, by photocopy or other means, without permission of the author. APPROVAL Name: Rutger Aldo Vos Degree: Doctor of Philosophy Title of Thesis: Inferring large phylogenies: The big tree problem Examining Committee: Chair: Dr. H. Hutter, Associate Professor Dr. A. Mooers, Assistant Professor, Senior Supervisor Department of Biological Sciences, S.F.U. Dr. W. Maddison, Professor Departments of Zoology and Botany, University of British Columbia Dr. B. Crespi, Professor Department of Biological Sciences, S.F.U. Dr. S. Graham, Assistant Professor Botanical Garden & Centre for Plant Research, and Department of Botany, University of British Columbia Public Examiner Dr. R. Page, Professor Division of Environmental and Evolutionary Biology, University of Glasgow External Examiner 12 May 2006 Date Approved SIMON FRASER UNI~ER~IW~~brary DECLARATION OF PARTIAL COPYRIGHT LICENCE The author, whose copyright is declared on the title page of this work, has granted to Simon Fraser University the right to lend this thesis, project or extended essay to users of the Simon Fraser University Library, and to make partial or single copies only for such users or in response to a request from the library of any other university, or other educational institution, on its own behalf or for one of its users. The author has further granted permission to Simon Fraser University to keep or make a digital copy for use in its circulating collection, and, without changing the content, to translate the thesislproject or extended essays, if technically possible, to any medium or format for the purpose of preservation of the digital work.
    [Show full text]
  • Preview Software Manual (PDF)
    BioGrapher Version 4.21 BETA for WinXP and Mac OS 10.5 (Workbook for Excel 2003, 2004, 2007) May 29, 2008 Excel spreadsheet design, features, and macros in the workbook copyright © 2003- 2008 by Rama Viswanathan ([email protected]). *****USE AT YOUR OWN RISK**** ------>PLEASE TAKE A MOMENT TO READ THE "READ ME FIRST FOR INSTALL" DOCUMENT FOR INSTALLATION INSTRUCTIONS AND KNOWN ISSUES--GraphViz calls will not work and graphs will not be drawn unless the GraphViz packages are installed in the right locations in the directory tree! All the files and executables needed by BioGrapher (including the AT&T GraphViz binaries) for WinXP/PC are now placed in a single self-contained BioGrapher FOLDER. In general, this folder should have been placed in a directory for which the user has full access (including write) privileges. BioGrapher works well off a flash drive. However, on a Mac running OS 10.5, the latest version 2.18 of the AT&T GraphViz package must have been installed (done when the BioGrapher installer script is executed), and this installation places the GraphViz binaries in /usr/local/bin and uses Mac system libraries (including XCODE libraries) in /usr/lib. It is also possible to use a special version of the BioGrapher software for older Mac OS (10.3.9 through 10.4.x) if you are able to download and access the older 2.16 version of GraphViz. See installation instructions for details. ----->This workbook uses MACROS, whose use MUST be enabled via the standard TOOLS--->MACRO--->SECURITY Excel menu item in Excel 2003 (or in Excel 2007 through the menu bar/ribbon alert that is displayed) when the workbook is first launched.
    [Show full text]
  • User Manual for Splitstree4 V4.17.1
    User Manual for SplitsTree4 V4.17.1 Daniel H. Huson and David Bryant June 28, 2021 Contents Contents 1 1 Introduction 4 2 Getting Started 5 3 Obtaining and Installing the Program5 4 Program Overview5 5 Splits, Trees and Networks7 6 Opening, Reading and Writing Files9 7 Estimating Distances9 8 Building and Processing Trees 10 9 Building and Drawing Networks 11 10 Main Window 11 1 10.1 Network Tab........................................ 11 10.2 Data Tab.......................................... 12 10.3 Tool bar........................................... 12 11 Graphical Interaction with the Network 12 12 Main Menus 13 12.1 File Menu.......................................... 13 12.2 Edit Menu.......................................... 14 12.3 View Menu......................................... 15 12.4 Data Menu......................................... 16 12.5 Distances Menu....................................... 17 12.6 Trees Menu......................................... 18 12.7 Network Menu....................................... 18 12.8 Analysis Menu....................................... 19 12.9 Draw Menu......................................... 20 12.10Window Menu....................................... 21 12.11Help Menu......................................... 21 12.12Configuring Methods.................................... 22 13 Popup Menus 22 14 Tool Bar 23 15 Data Entry Dialog 23 16 Pipeline Window 23 16.1 Taxa Tab.......................................... 24 16.2 Unaligned Tab....................................... 24 16.3 Characters Tab......................................
    [Show full text]
  • Reconciliation of Gene and Species Trees with Polytomies
    Vol. 00 no. 00 2011 Doc-StartReconciliationBIOINFORMATICS of Gene and Species Trees With Pages 1–10 Polytomies Yu Zheng 1, Taoyang Wu 1, Louxin Zhang 1 ∗ 1Department of Mathematics, Nat’l University of Singapore, 10 Lower Kent Ridge, S’pore 119076 Received on XXXXX; revised on XXXXX; accepted on XXXXX Associate Editor: XXXXXXX ABSTRACT neofunctionalization. Ortholog identification is the first task of Motivation: Millions of genes in the modern species belong to only almost every comparative genomic study since orthologs are used thousands of gene families. Genes duplicate and are lost during to infer the pattern of gene gain and loss, the mode of signaling evolution. A gene family includes instances of the same gene in pathway evolution, and the correspondence between genotype and different species and duplicate genes in the same species. Two phenotype. genes in different species are ortholog if their common ancestor lies Genes are gained through duplication and horizontal gene transfer in the most recent common ancestor of the species. Because of and lost via deletion and pesudogenization throughout evolution. complex gene evolutionary history, ortholog identification is a basic Identifying orthologs is essentially to find out how genes evolved. but difficult task in comparative genomics. A key method for the task Since past evolutionary events cannot be observed directly, we is to use an explicit model of the evolutionary history of the genes have to infer these events from the gene sequences available today. being studied, called the gene (family) tree. It compares the gene Therefore, ortholog identification is never an easy task. tree with the evolutionary history of the species in which the genes A key method for ortholog identification is to use an explicit reside, called the species tree, using the procedure known as tree model of the evolutionary history of the genes subject to study, in reconciliation.
    [Show full text]