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CHARACTERIZATION OF VOLATILE PYRETHROIDS FOR MOSQUITO MANAGEMENT By CHRISTOPHER STEPHEN BIBBS A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2019 © 2019 Christopher Stephen Bibbs To my children, Aerith Liesel Bibbs and Griffin Roderick Bibbs, for being the reason I seek to better myself. To my parents, for enabling my fascination with insects. To my advisors, from my first days as an undergraduate to my last days completing my doctorate, whose friendship changed the way I think about the world. And to all the wonderful people who saw fit to “pay it forward” throughout my journey, even though I was owed nothing. ACKNOWLEDGMENTS I greatly appreciate the Anastasia Mosquito Control District (AMCD) of St. Johns County for allowing me to pursue my education with their employee degree work-study support. If it were not for the policies enacted by the AMCD board of commissioners, the high-quality facilities opened to my research, and most importantly, the strong belief in higher education emphasized by District director Dr. Rui-De Xue, none of this would have been possible. I originally rejected the idea of going to school after my Master of Science. Dr. Xue did not give up on my potential to go farther. Despite the struggles, I am better for it. I hope the work enclosed in this dissertation has helped improve the visibility of AMCD as a profound public health research entity in Florida. Various staff and friends have all walked with me on this path. A special debt of gratitude is owed to former biologist Alice Fulcher, former Education Specialist Jodi Scott-Fiorenzano, Operations Manager Kay Gaines, Data Manager/Business Manager Richard Weaver, former Field Biologist Michael Smith, senior Mechanic James Wynn, former Biological Technician Jennifer Gibson-Corrado, former head of surveillance Dr. Daniel Dixon, Assistant Supervisor Dena Autry, Education Specialist Molly Clark, as well as all the Mosquito Control Technicians, Seasonal Inspector/Sprayers, Visiting Scientists, and Interns. These people have enriched my life, offered their guidance, and taught me public health as a passion and a career. Funding for this research was leveraged in large part from the grants awarded by the Florida Department of Agriculture and Consumer Services, with special mention to National Science Foundation’s Center for Arthropod Management Technology (NSF-CAMTech), whose Industry Advisory Board helped me on my path. When I started this rocky road, I lacked the experience, the notoriety, and the skills to receive even a single grant despite years of attempts prior to my PhD program. Aside from my advisor, Dr. Phillip Kaufman, whom I learned from 4 long before even graduating with my Bachlelor of Science, the contributions of Dr. Jeff Bloomquist, Dr. Daniel Hahn, and Dr. Christopher Batich cannot be understated. Some believed that I may have chosen some of the most difficult committee members possible out of my options at the Entomology and Nematology Department. In my belief, if I was not good enough for them then I was not good enough for myself. We wrote these grant proposals together. We received this funding together. This dissertation was published by all of us together. Your experience, values, and fortitude are part of my own now. My sponsorship as a student has been a joint effort by the University of Florida Entomology and Nematology Department (UF), Florida Mosquito Control Association (FMCA), American Mosquito Control Association (AMCA), the Florida Entomological Society (FES), and the Entomological Society of America (ESA). The entomology department at UF, through Dr. Heather McAuslane, awarded the Grinter Fellowship three years to my PhD program. This great honor provided stability as I sought additional funding to complete my program. The FMCA memorial scholarships of Cy Lesser and T. Wainwright, bestowed graciously by public health role models such as Dr. Roxanne Connelly, Chris Lesser of Manatee County Mosquito Control District, Aaron Lloyd of Pasco County Mosquito Control District, and many others was a powerful support system that ensured I could make it to the finish line. The travel grants, student competition awards, and general student support networks of AMCA, FES, and ESA continuously proved how much the membership base was looking out for me. Dr.’s Ary Faraji, Isik Unlu, Seth Britch, Mike Breidenbaugh, Michael Turrell, Stan Cope, Kristy Burkhalter, Rajeev Vaidyanathan, Mustapha Debboun, Heather McAuslane, Blair Siegfried, Jawwad Qureshi, and many other members have all been a wonderful part of my life as they have demonstrated how to be a good entomologists, even better scientists, and great human beings. 5 This series of projects was made possible by collaborations through the United States Department of Agriculture – Agricultural Research Service – Center for Medical, Agricultural, and Veterinary entomology, McLoughlin Gormley King Corporation (MGK), Sumitomo Corporation, and Sigma Scientific, LLC. Dr.’s Dan Kline, Bradley Willenberg, Maia Tsikolia, Uli Bernier, Nurhayat Tabanca and Ken Linthicum have all been wonderful partners as we learn how to advance vector management. Dr. Jennifer Williams, Hitoshi Kawada, Takao Ishiwatari, and others affiliated with MGK and Sumitomo have been insightful collaborators that provided the tools that were needed to advance our understanding of my research topics. And finally, Rudolph Strohschein and Jim Estaver have been inspirational creatives whose ingenuity and deep history with scientific process will be a lasting influence in my career. They may declare themselves just two hicks in Micanopy, FL, but Sigma will always be a beautiful cover for such diamonds in the rough. To my advisors through time, Dr. Phillip Kaufman, Dr. Dawn Gouge, and Dr. Rebecca Baldwin: this dissertation is proof your faith was not misplaced. I met all three of you when I was only an undergraduate at the University of Florida. I am glad that I have been able to keep you in my life and learn from you both before and after my various stepping stones. My very first attempt at being an entomologist was only successful because Dr. Baldwin rescued me, mentored me, and looked out for me as I navigated college life and my early learning as a researcher. When no other graduate program I applied for would give me a chance, Dr. Gouge offered a place in her piece of Arizona because she believed I could succeed. I hope I can give back to you even now that I have left your laboratory. Since the beginning, my greatest professional role model has always been Dr. Phillip Kaufman. When I did not know what to do, I would think back to my crowded, tiny work spaces shared with Dr. Dale Halbritter and Dr. Chris 6 Holderman and ask myself what Kaufman would say I needed to do. I emulated you and your values from the beginning. When I did what I thought Dr. Kaufman would tell me, I aced my medical, veterinary, biosecurity, and forensic entomology subjects. When I did what I thought Dr. Kaufman would tell me, I completed my Master of Science at University of Arizona on time, with publications in print, and a job offer at AMCD waiting for me out the door. When I did what I thought Dr. Kaufman needed me to do, I accomplished the work in my PhD program despite being a non-traditional student, working a full-time job, and not having the benefit of being surrounded by peer students at a University office every day. I was always part of Dr. Kaufman’s laboratory, even if I did not occupy a desk. Thank you for being there. 7 TABLE OF CONTENTS page ACKNOWLEDGMENTS ........................................................................................................... 4 LIST OF TABLES .................................................................................................................... 11 LIST OF FIGURES .................................................................................................................. 12 LIST OF ABBREVIATIONS.................................................................................................... 15 ABSTRACT ............................................................................................................................. 18 CHAPTER 1 INTRODUCTION.................................................................................................................. 20 1.1 Preface ......................................................................................................................... 20 1.2 Literature Review ......................................................................................................... 24 1.2.1 Repellency .......................................................................................................... 24 1.2.2 Acute Symptoms and Toxicity ............................................................................ 29 1.2.3 Sub-Lethal Effects .............................................................................................. 33 1.2.4 Evaluation Methods ............................................................................................ 38 1.3 Objectives .................................................................................................................... 42 1.4 Discolsures ................................................................................................................... 43 1.5 Figures ........................................................................................................................