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Effective Removal of Alkanes and Polycyclic Aromatic Hydrocarbons by Bacteria from Soil Chronically Exposed to Crude Petroleum Oil
Effective Removal of Alkanes and Polycyclic Aromatic Hydrocarbons by Bacteria from Soil Chronically Exposed to Crude Petroleum Oil Eman Afkar ( [email protected] ) Dept. of Basic Sciences, Preparatory Year Deanship (PYD), King Faisal University, Al-Ahsa, 31982, Saudi Arabia. https://orcid.org/0000-0002-7442-4880 Aly M. Hafez Dept. of Chemistry, College of Science, King Faisal University, Al-Ahsa, 31982, Saudi Arabia. Rashid I.H. Ibrahim Dept. of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, 31982, Saudi Arabia. Dept. of Botany, Faculty of Science, University of Khartoum, PO Box 321, PC 11115, Sudan Munirah Aldayel Dept. of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, 31982, Saudi Arabia. Research Keywords: n-alkanes, GC-MS, crude oil degradation, bacteria, contaminants, hydrocarbons Posted Date: February 16th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-207407/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/18 Abstract In this study, two bacterial strains isolated from an oil-contaminated soil, designated as AramcoS2 and AramcoS4 were able to degrade crude oil, long-chain n-alkanes of C10 to C20; (n-decane, n-undecane, n- dodecane, n-tridecane, n-tetradecane, n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane n- nonadecane, and n-eicosane) and polycyclic aromatic hydrocarbons (PAHs) including biphenyl, naphthalene, and anthracene. Gas chromatography-mass spectrometry (GC-MS) technique was conducted to analyze and identify the crude oil residues after biodegradation. AramcoS2 and AramcoS4 were able to reduce the concentration of long-chain n-alkanes of C10-C20 eciently on average by 77% of the original concentration. -
A Confinement Strategy to Prepare N-Doped Reduced Graphene Oxide
Electronic Supplementary Material (ESI) for Nanoscale. This journal is © The Royal Society of Chemistry 2019 Supporting Information A confinement strategy to prepare N-doped reduced graphene oxide foams with desired monolithic structures for supercapacitors Daoqing Liu,a,b,# Qianwei Li, *c,# Si Li, a,b Jinbao Hou,d Huazhang Zhao *a,b a Department of Environmental Engineering, Peking University, Beijing 100871, China b The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China c State Key Laboratory of Heavy Oil Processing, Beijing Key Laboratory of Oil and Gas Pollution Control, China University of Petroleum, 18 Fuxue Road, Changping District, Beijing 102249, China d College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China # Co-first author. * Corresponding author. Tel/fax: +86-10-62758748; email address: [email protected] (H.Z. Zhao). S1 Experimental Methods 1. Preparation of graphite oxide (GO) Graphite oxide was prepared from natural graphite (Jinglong Co., Beijing, China) according to 1 a modified Hummers method : 120 mL 98 wt% H2SO4 was poured into a beaker containing a mixture of 5 g natural graphite and 2.5 g NaNO3, and then the mixture was stirred in an ice bath for 30 min. 15 g KMnO4 was added slowly into the mixture, which was allowed to react for 2 h at a temperature no more than 20°C. Then, the temperature was risen to 35°C, and the reaction was performed for another 2 h. After that, the reactant mixture was poured slowly into 360 mL distilled water under violent stirring condition so as to control the temperature no more than 90°C, followed by further reaction at 75°C for 1 h. -
Sections-A, B and C
Science IX Sample Paper 7 Solved www.rava.org.in CLASS IX (2019-20) SCIENCE (CODE 086) SAMPLE PAPER-7 Time : 3 Hours Maximum Marks : 80 General Instructions : (i) The question paper comprises of three sections-A, B and C. Attempt all the sections. (ii) All questions are compulsory. (iii) Internal choice is given in each sections. (iv) All questions in Section A are one-mark questions comprising MCQ, VSA type and assertion-reason type questions. They are to be answered in one word or in one sentence. (v) All questions in Section B are three-mark, short-answer type questions. These are to be answered in about 50-60 words each. (vi) All questions in Section C are five-mark, long-answer type questions. These are to be answered in about 80-90 words each. (vii) This question paper consists of a total of 30 questions. 6. Who proposed the fluid mosaic model of protoplasm? [1] Section - A (a) Singer and Nicolson (b) Watson and Crick (c) Robert Hook (d) Robert Brown 1. Which of the following actions a force can do? [1] (a) Can move a stationary object. Ans : (a) Singer and Nicolson. (b) Can stop a moving object. or (c) Can change the speed of a moving object. Which of the following are complex tissues? (d) All of the above. (a) Xylem and Phloem Ans : (d) All of the above (b) Collenchyma and Sclerenchyma (c) Parenchyma and Collenchyma 2. Ozone layer protects us from which one of the (d) Xylem and Parenchyma following? [1] (a) X- rays. -
Measurements of Higher Alkanes Using NO Chemical Ionization in PTR-Tof-MS
Atmos. Chem. Phys., 20, 14123–14138, 2020 https://doi.org/10.5194/acp-20-14123-2020 © Author(s) 2020. This work is distributed under the Creative Commons Attribution 4.0 License. Measurements of higher alkanes using NOC chemical ionization in PTR-ToF-MS: important contributions of higher alkanes to secondary organic aerosols in China Chaomin Wang1,2, Bin Yuan1,2, Caihong Wu1,2, Sihang Wang1,2, Jipeng Qi1,2, Baolin Wang3, Zelong Wang1,2, Weiwei Hu4, Wei Chen4, Chenshuo Ye5, Wenjie Wang5, Yele Sun6, Chen Wang3, Shan Huang1,2, Wei Song4, Xinming Wang4, Suxia Yang1,2, Shenyang Zhang1,2, Wanyun Xu7, Nan Ma1,2, Zhanyi Zhang1,2, Bin Jiang1,2, Hang Su8, Yafang Cheng8, Xuemei Wang1,2, and Min Shao1,2 1Institute for Environmental and Climate Research, Jinan University, 511443 Guangzhou, China 2Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality, 511443 Guangzhou, China 3School of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), 250353 Jinan, China 4State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 510640 Guangzhou, China 5State Joint Key Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, 100871 Beijing, China 6State Key Laboratory of Atmospheric Boundary Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese -
Supporting Information for Modeling the Formation and Composition Of
Supporting Information for Modeling the Formation and Composition of Secondary Organic Aerosol from Diesel Exhaust Using Parameterized and Semi-explicit Chemistry and Thermodynamic Models Sailaja Eluri1, Christopher D. Cappa2, Beth Friedman3, Delphine K. Farmer3, and Shantanu H. Jathar1 1 Department of Mechanical Engineering, Colorado State University, Fort Collins, CO, USA, 80523 2 Department of Civil and Environmental Engineering, University of California Davis, Davis, CA, USA, 95616 3 Department of Chemistry, Colorado State University, Fort Collins, CO, USA, 80523 Correspondence to: Shantanu H. Jathar ([email protected]) Table S1: Mass speciation and kOH for VOC emissions profile #3161 3 -1 - Species Name kOH (cm molecules s Mass Percent (%) 1) (1-methylpropyl) benzene 8.50×10'() 0.023 (2-methylpropyl) benzene 8.71×10'() 0.060 1,2,3-trimethylbenzene 3.27×10'(( 0.056 1,2,4-trimethylbenzene 3.25×10'(( 0.246 1,2-diethylbenzene 8.11×10'() 0.042 1,2-propadiene 9.82×10'() 0.218 1,3,5-trimethylbenzene 5.67×10'(( 0.088 1,3-butadiene 6.66×10'(( 0.088 1-butene 3.14×10'(( 0.311 1-methyl-2-ethylbenzene 7.44×10'() 0.065 1-methyl-3-ethylbenzene 1.39×10'(( 0.116 1-pentene 3.14×10'(( 0.148 2,2,4-trimethylpentane 3.34×10'() 0.139 2,2-dimethylbutane 2.23×10'() 0.028 2,3,4-trimethylpentane 6.60×10'() 0.009 2,3-dimethyl-1-butene 5.38×10'(( 0.014 2,3-dimethylhexane 8.55×10'() 0.005 2,3-dimethylpentane 7.14×10'() 0.032 2,4-dimethylhexane 8.55×10'() 0.019 2,4-dimethylpentane 4.77×10'() 0.009 2-methylheptane 8.28×10'() 0.028 2-methylhexane 6.86×10'() -
American Version
COMPENDIUM OF INTERNATIONAL METHODS OF ANALYSIS-OIV Detection of preservatives and fermentation inhibitors Method OIV-MA-AS4-02E Type IV method Detection of preservatives and fermentation inhibitors Method A 35 modified by resolution Oeno 6/2006 1. Examination of dehydroacetic acid 1.1 Principle Wine acidified with sulfuric acid is extracted with a mixture of equal parts of diethyl ether and petroleum ether. After evaporation of the solvent, the extract, recovered with a small quantity of 96% ethanol (v/v) is deposited on a thin layer of polyamide and silica gel with fluorescent indicator and subjected to the action of the mobile solvent (benzene-acetone-acetic acid). The dehydroacetic acid is identified and characterized by ultraviolet examination of the chromatogram. 1.2 Apparatus 1.2.1 Equipment for thin layer chromatography 1.2.2 Oven 1.2.3 Rotary evaporator 1.2.4 UV lamp 254 nm. 1.3 Reagents 1.3.1 Diethyl ether 1.3.2 Petroleum ether (boiling point 40 °C) 1.3.3 Methanol 1.3.4 Sulfuric acid, 20% (v/v) 1.3.5 Anhydrous sodium sulfate. 1.3.6 Ethanol, 96% (v/v) . 1.3.7 Chromatographic separation layer: 10 g polyamide powder with fluorescent indicator(e.g. polyamide DC II UV254 from Macherey-Nagel) mixed vigorously with 60 mL methanol. Add while stirring, 10 ml of water and 10ml of silica gel (with fluorescent indicator), e.g. Kiesselgel GF254 Merck. Spread this mixture on 5 plates (200 x 200 mm) to a thickness of 0.25 mm. Dry the plates at room temperature for 30 minutes, then place in a 70°C oven for 10 min. -
Download Report Document
AEAT\EPSC-0044 Issue 1 Development of Species Profiles for UK Emissions of VOCs A report produced for the Department of the Environment, Transport & the Regions M E Jenkin, N R Passant & H J Rudd April 2000 AEAT\EPSC-0044 Issue 1 Development of Species Profiles for UK Emissions of VOCs A report produced for the Department of the Environment, Transport & the Regions M E Jenkin, N R Passant & H J Rudd April 2000 AEAT\EPSC-0044 Issue 1 Title Development of Species Profiles for UK Emissions of VOCs Customer DETR Air & Environmental Quality Division Customer reference EPG 1/3/134 Confidentiality, copyright and reproduction This document has been prepared by AEA Technology plc in connection with a contract to supply goods and/or services and is submitted only on the basis of strict confidentiality. The contents must not be disclosed to third parties other than in accordance with the terms of the contract. File reference ED20699106 Report number AEAT\EPSC-0044 Report status Issue 1 E6 Culham Abingdon Oxon OX14 3ED Telephone 01235 463977 Facsimile 01235 463001 AEA Technology is the trading name of AEA Technology plc AEA Technology is certificated to BS EN ISO9001:(1994) Name Signature Date Author Michael Jenkin Neil Passant Howard Rudd Reviewed by John Branson Approved by Mike Woodfield AEA Technology ii AEAT\EPSC-0044 Issue 1 Contents 1 Introduction 1 2 Improvements to Speciation 2 2.1 OVERVIEW 2 2.2 CHANGES TO SPECIES PROFILES 2 2.2.1 Solvent xylene 2 2.2.2 Industrial paints 3 2.2.3 Printing processes 3 2.2.4 Agrochemicals 4 2.2.5 Revisions -
Safety Data Sheet
SAFETY DATA SHEET Flammable Liquid Mixture: Docosane / Dodecane / Eicosane / Hexacosane / Hexadecane / Hexane / N-Decane / N-Heptane / N-Nonane / N-Octadecane / N- Octane / Octacosane / Tetracosane / Tetradecane Section 1. Identification GHS product identifier : Flammable Liquid Mixture: Docosane / Dodecane / Eicosane / Hexacosane / Hexadecane / Hexane / N-Decane / N-Heptane / N-Nonane / N-Octadecane / N-Octane / Octacosane / Tetracosane / Tetradecane Other means of : Not available. identification Product use : Synthetic/Analytical chemistry. SDS # : 019735 Supplier's details : Airgas USA, LLC and its affiliates 259 North Radnor-Chester Road Suite 100 Radnor, PA 19087-5283 1-610-687-5253 24-hour telephone : 1-866-734-3438 Section 2. Hazards identification OSHA/HCS status : This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Classification of the : FLAMMABLE LIQUIDS - Category 1 substance or mixture SKIN CORROSION/IRRITATION - Category 2 SERIOUS EYE DAMAGE/ EYE IRRITATION - Category 2A TOXIC TO REPRODUCTION (Fertility) - Category 2 TOXIC TO REPRODUCTION (Unborn child) - Category 2 SPECIFIC TARGET ORGAN TOXICITY (SINGLE EXPOSURE) (Respiratory tract irritation) - Category 3 SPECIFIC TARGET ORGAN TOXICITY (SINGLE EXPOSURE) (Narcotic effects) - Category 3 SPECIFIC TARGET ORGAN TOXICITY (REPEATED EXPOSURE) - Category 2 AQUATIC HAZARD (ACUTE) - Category 2 AQUATIC HAZARD (LONG-TERM) - Category 1 GHS label elements Hazard pictograms : Signal word : Danger Hazard statements : Extremely flammable liquid and vapor. May form explosive mixtures in Air. Causes serious eye irritation. Causes skin irritation. May cause respiratory irritation. Suspected of damaging fertility or the unborn child. May cause drowsiness and dizziness. May cause damage to organs through prolonged or repeated exposure. Very toxic to aquatic life with long lasting effects. Precautionary statements General : Read label before use. -
SDS Contains All of the Information Required by the HPR
SAFETY DATA SHEET Preparation Date: 4/1/2014 Revision date 6/24/2019 Revision Number: G2 1. IDENTIFICATION Product identifier Product code: P1040 Product Name: PETROLEUM ETHER, B.R. 20 DEG -40 DEG C Other means of identification Synonyms: Benzine (light petroleum distillate) Benzoline Canadol Ligroin Painters naphtha Refined solvent naphtha CAS #: 8032-32-4 RTECS # OI6180000 CI#: Not available Recommended use of the chemical and restrictions on use Recommended use: Solvent. Uses advised against No information available Supplier: Spectrum Chemical Mfg. Corp 14422 South San Pedro St. Gardena, CA 90248 (310) 516-8000 Order Online At: https://www.spectrumchemical.com Emergency telephone number Chemtrec 1-800-424-9300 Contact Person: Tom Tyner (USA - West Coast) Contact Person: Ibad Tirmiz (USA - East Coast) 2. HAZARDS IDENTIFICATION Classification This chemical is considered hazardous by the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200) Considered a dangerous substance or mixture according to the Globally Harmonized System (GHS) Acute toxicity - Inhalation (Gases) Category 4 Acute toxicity - Inhalation (Vapors) Category 4 Skin corrosion/irritation Category 2 Serious eye damage/eye irritation Category 2A Specific target organ toxicity (single exposure) Category 3 Specific target organ toxicity (repeated exposure) Category 2 Aspiration toxicity Category 1 Flammable liquids Category 2 Label elements Product code: P1040 Product name: PETROLEUM ETHER, Page 1 / 13 B.R. 20 DEG -40 DEG C Danger Hazard statements Harmful if inhaled Causes -
Vapor Pressures and Vaporization Enthalpies of the N-Alkanes from 2 C21 to C30 at T ) 298.15 K by Correlation Gas Chromatography
BATCH: je1a04 USER: jeh69 DIV: @xyv04/data1/CLS_pj/GRP_je/JOB_i01/DIV_je0301747 DATE: October 17, 2003 1 Vapor Pressures and Vaporization Enthalpies of the n-Alkanes from 2 C21 to C30 at T ) 298.15 K by Correlation Gas Chromatography 3 James S. Chickos* and William Hanshaw 4 Department of Chemistry and Biochemistry, University of MissourisSt. Louis, St. Louis, Missouri 63121 5 6 The temperature dependence of gas chromatographic retention times for n-heptadecane to n-triacontane 7 is reported. These data are used to evaluate the vaporization enthalpies of these compounds at T ) 298.15 8 K, and a protocol is described that provides vapor pressures of these n-alkanes from T ) 298.15 to 575 9 K. The vapor pressure and vaporization enthalpy results obtained are compared with existing literature 10 data where possible and found to be internally consistent. Sublimation enthalpies for n-C17 to n-C30 are 11 calculated by combining vaporization enthalpies with fusion enthalpies and are compared when possible 12 to direct measurements. 13 14 Introduction 15 The n-alkanes serve as excellent standards for the 16 measurement of vaporization enthalpies of hydrocarbons.1,2 17 Recently, the vaporization enthalpies of the n-alkanes 18 reported in the literature were examined and experimental 19 values were selected on the basis of how well their 20 vaporization enthalpies correlated with their enthalpies of 21 transfer from solution to the gas phase as measured by gas 22 chromatography.3 A plot of the vaporization enthalpies of 23 the n-alkanes as a function of the number of carbon atoms 24 is given in Figure 1. -
Table 2. Chemical Names and Alternatives, Abbreviations, and Chemical Abstracts Service Registry Numbers
Table 2. Chemical names and alternatives, abbreviations, and Chemical Abstracts Service registry numbers. [Final list compiled according to the National Institute of Standards and Technology (NIST) Web site (http://webbook.nist.gov/chemistry/); NIST Standard Reference Database No. 69, June 2005 release, last accessed May 9, 2008. CAS, Chemical Abstracts Service. This report contains CAS Registry Numbers®, which is a Registered Trademark of the American Chemical Society. CAS recommends the verification of the CASRNs through CAS Client ServicesSM] Aliphatic hydrocarbons CAS registry number Some alternative names n-decane 124-18-5 n-undecane 1120-21-4 n-dodecane 112-40-3 n-tridecane 629-50-5 n-tetradecane 629-59-4 n-pentadecane 629-62-9 n-hexadecane 544-76-3 n-heptadecane 629-78-7 pristane 1921-70-6 n-octadecane 593-45-3 phytane 638-36-8 n-nonadecane 629-92-5 n-eicosane 112-95-8 n-Icosane n-heneicosane 629-94-7 n-Henicosane n-docosane 629-97-0 n-tricosane 638-67-5 n-tetracosane 643-31-1 n-pentacosane 629-99-2 n-hexacosane 630-01-3 n-heptacosane 593-49-7 n-octacosane 630-02-4 n-nonacosane 630-03-5 n-triacontane 638-68-6 n-hentriacontane 630-04-6 n-dotriacontane 544-85-4 n-tritriacontane 630-05-7 n-tetratriacontane 14167-59-0 Table 2. Chemical names and alternatives, abbreviations, and Chemical Abstracts Service registry numbers.—Continued [Final list compiled according to the National Institute of Standards and Technology (NIST) Web site (http://webbook.nist.gov/chemistry/); NIST Standard Reference Database No. -
Section 2. Hazards Identification OSHA/HCS Status : This Material Is Considered Hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200)
SAFETY DATA SHEET Flammable Liquefied Gas Mixture: 2-Methylpentane / 2,2-Dimethylbutane / 2, 3-Dimethylbutane / 3-Methylpentane / Benzene / Carbon Dioxide / Decane / Dodecane / Ethane / Ethyl Benzene / Heptane / Hexane / Isobutane / Isooctane / Isopentane / M- Xylene / Methane / N-Butane / N-Pentane / Neopentane / Nitrogen / Nonane / O- Xylene / Octane / P-Xylene / Pentadecane / Propane / Tetradecane / Toluene / Tridecane / Undecane Section 1. Identification GHS product identifier : Flammable Liquefied Gas Mixture: 2-Methylpentane / 2,2-Dimethylbutane / 2, 3-Dimethylbutane / 3-Methylpentane / Benzene / Carbon Dioxide / Decane / Dodecane / Ethane / Ethyl Benzene / Heptane / Hexane / Isobutane / Isooctane / Isopentane / M- Xylene / Methane / N-Butane / N-Pentane / Neopentane / Nitrogen / Nonane / O-Xylene / Octane / P-Xylene / Pentadecane / Propane / Tetradecane / Toluene / Tridecane / Undecane Other means of : Not available. identification Product type : Liquefied gas Product use : Synthetic/Analytical chemistry. SDS # : 018818 Supplier's details : Airgas USA, LLC and its affiliates 259 North Radnor-Chester Road Suite 100 Radnor, PA 19087-5283 1-610-687-5253 24-hour telephone : 1-866-734-3438 Section 2. Hazards identification OSHA/HCS status : This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Classification of the : FLAMMABLE GASES - Category 1 substance or mixture GASES UNDER PRESSURE - Liquefied gas SKIN IRRITATION - Category 2 GERM CELL MUTAGENICITY - Category 1 CARCINOGENICITY - Category 1 TOXIC TO REPRODUCTION (Fertility) - Category 2 TOXIC TO REPRODUCTION (Unborn child) - Category 2 SPECIFIC TARGET ORGAN TOXICITY (SINGLE EXPOSURE) (Narcotic effects) - Category 3 SPECIFIC TARGET ORGAN TOXICITY (REPEATED EXPOSURE) - Category 2 AQUATIC HAZARD (ACUTE) - Category 2 AQUATIC HAZARD (LONG-TERM) - Category 1 GHS label elements Hazard pictograms : Signal word : Danger Hazard statements : Extremely flammable gas. May form explosive mixtures with air.