TMEM59 Antibody Cat. No.: 6063

TMEM59 Antibody

Immunohistochemistry of TMEM59 in human kidney Immunofluorescence of TMEM59 in human kidney tissue tissue with TMEM59 antibody at 2.5 μg/mL. with TMEM59 antibody at 20 μg/mL.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%), HOMOLOGY: Mouse: (94%)

TMEM59 antibody was raised against a 16 amino acid synthetic peptide near amino terminus of human TMEM59. IMMUNOGEN:

The immunogen is located within amino acids 80 - 130 of TMEM59.

TESTED APPLICATIONS: ELISA, IF, IHC-P, WB

October 1, 2021 1 https://www.prosci-inc.com/tmem59-antibody-6063.html TMEM59 antibody can be used for detection of TMEM59 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

POSITIVE CONTROL: 1) Cat. No. 1305 - Human Kidney Tissue Lysate

2) Cat. No. 10-401 - Human Kidney Tissue Slide

Properties

PURIFICATION: TMEM59 Antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: TMEM59 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

TMEM59 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: TMEM59

TMEM59 Antibody: C1orf8, HSPC001, C1orf8, UNQ169/PRO195, Transmembrane ALTERNATE NAMES: 59, Liver membrane-bound protein

ACCESSION NO.: NP_004863

PROTEIN GI NO.: 20070191

GENE ID: 9528

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

October 1, 2021 2 https://www.prosci-inc.com/tmem59-antibody-6063.html TMEM59 Antibody: Processing of the precursor protein (APP) by two different proteases, called alpha- and beta-secretase, is a central regulatory event in the generation of the peptide (Abeta), which has a key role in Alzheimer disease (AD) BACKGROUND: pathogenesis. TMEM59, a Golgi-localized protein, modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several such as APP, BACE1, SEAP or PRNP. It inhibits APP transport and shedding.

1) Schöbel S, Neumann S, Seed B, et al. Expression cloning screen for modifiers of amyloid REFERENCES: precursor protein shedding. Int. J. Dev. Neurosci.2006; 24:141-8.

2) Schöbel S, Neumann S, Hertweck M, et al. A novel sorting nexin modulates endocytic trafficking and alpha-secretase cleavage of the amyloid precursor protein. J. Biol. Chem.2008; 283:14257-68.

3) Ullrich S, Münch A, Neumann S, et al. The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein. J. Biol. Chem.2010; 285:20664-74.

4) Elson GC, de Coignac AB, Aubry JP, et al. BSMAP, a novel protein expressed specifically in the brain whose is localized on 19p12. Biochem. Biophys. Res. Commun.1999; 264:55-62.

ANTIBODIES FOR RESEARCH USE ONLY.

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