21.10.2014SIGIRR (CT) (pAb)

Rabbit Anti -Human/Mouse SIGIRR (TLR Inhibitor)

Instruction Manual

Catalog Number PK-AB718-3367 Synonyms SIGIRR Antibody: Description SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily. Members of this family are defined by the presence of an intracellular Toll-IL-1R (TIR) domain. The Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during and serve as an important link between the innate and adaptive immune responses. SIGIRR was originally identified through database analysis and was shown to have only one Ig domain as opposed to the normal three Ig folds seen in the TIR family. Similar to ST2, another TIR family member, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor signaling. However, SIGIRR inhibits TLR-IL-1R signaling by dimerizing with TLR4, TLR5, TLR9, and IL-1R. It also associates with the down-stream TLR signaling IRAK and TRAF6 in an IL-1- dependent fashion. Quantity 100 µg Source / Host Rabbit Immunogen Rabbit polyclonal SIGIRR antibody was raised against a synthetic peptide corresponding to 15 amino acids at the C-terminus of mouse SIGIRR (Genbank accession No. CAG33619). Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, ICC |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually.h application have to be determined indivudually. SIGIRR antibody can be used for the detection of SIGIRR by Western blot at 0.5 to 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. Images Available upon request. References Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. Thomassen E, Renshaw BR, and Sims JE. Identification and characterization of SIGIRR, a molecule representing a novel subtype of the IL-1R superfamily. 1999; 11:389-99. Wald D, Qin J, Zhao Z, et al. SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat. Immunol. 2003; 4:920-7. Images Available upon request. Related Products Blocking Peptide, Cat. No. PK-AB718-3367P A549 Cell Lysate, Cat. No. PK-AB718-1203 ST2 Antibody (NT), Cat. No. PK-AB718-3363; TRAF6 Antibody (CT), Cat. No. PK-AB718-3129 TLR4 Antibody (NT), Cat. No. PK-AB718-3141 FOR IN VITRO RESEARCHIRAK Antibody USE ONLY. (CT), Cat. NOT No. FOR PK-AB718-1007 DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

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