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PRKCSH Rabbit pAb
Catalog No.: A4045
Basic Information Background
Catalog No. This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing A4045 enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been Observed MW associated with the autosomal dominant polycystic liver disease. Alternative splicing 80KDa results in multiple transcript variants.
Calculated MW 59kDa
Category Primary antibody
Applications WB
Cross-Reactivity Human, Mouse, Rat
Recommended Dilutions Immunogen Information
WB 1:500 - 1:1000 Gene ID Swiss Prot 5589 P14314
Immunogen A synthetic Peptide of human PRKCSH
Synonyms PRKCSH;AGE-R2;G19P1;GIIB;PCLD;PCLD1;PKCSH;PLD1;VASAP-60
Contact Product Information
www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification
Storage Store at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,pH7.3. Validation Data
Western blot analysis of extracts of various cell lines, using PRKCSH antibody (A4045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Western blot analysis of extracts of Mouse lung, using PRKCSH antibody (A4045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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