Revision: 1 Effective Date: 5/13/2019 hours. required. if carefully Mix agar. molten the over pouring before dish, Petri the to in used) be should dilutions (serial sample test the of 1ml inoculate method, plate pour the Using Procedure Test 4. 3. 2. 1. Preparation toSDS Refer Precaution specifications. performance to meet required as supplemented and/or adjusted be may Formula Final pH: Agar 1.08 Monooleate Polyoxyethylenesorbitan Phosphate Hydrogen Dipotassium 0.05 Tetrahydrate Sulphate Manganese 0.2 Sulphate Heptahydrate Magnesium Acetate Sodium 2.0 Citrate Triammonium 4.0 Extract Yeast 10.0 Extract Meat Casein of Digest Enzymatic Typical Formulation recommended +/ 5.7 limits ster Occasionally, groups. other organism of number a inhibiting whilst lactobacilli of citrate growth allows pH, ammonium low at acetate. Used sodium citrate and ammonium by is provided & molds streptococci against Selectivity sulphates and manganese magnesium monooleate, polyoxyethylenesorbitan whilst yeast & extracts meat glucose, casein, of digest enzymatic by is provided Nutrition Agar. Juice Tomato for a as substitute is intended and various sources spp.from of enumeration and ISO cultivation the for & Sharpe Rogosa de Man, by 1960 in developed was originally Agar MRS to according bacteria Description acid lactic mesophilic of enumeration the for medium 15214:1998 a is Agar MRS Use Intended Sheet Specification Technical

C minutes. 15 for 121°C at Autoclave medium. the dissolve completely to minute one boil for and agitation frequent with Heat Suspend

ool This original formulation has been adapted and adjusted to pH 5.7 according to ISO 15214:1998. to ISO according 5.7 pH to adjusted and adapted been has formulation original This

to 45 to 5.7

-

, Surface inoculations may also may inoculations Surface

0.1. In these rare cases rare these In 0.1. and and

± 0. ± 70

-

50°C.

grams of the medium the medium of grams 1 is not intended for use in the diagnosis of diagnosis the in use for intended not is ili

at 25°C 25°C at z ation of this medium at 121 this medium of ation

MRS AgarMRS adjustment of the medium using acetic acid or sodium hydroxide is hydroxide or sodium using acetic acid the medium of adjustment in

one

10.0 15.5 20.0

5.0 2.0 be used. be

liter of purified water. purified of liter

g/L g/L g/L g/L g/L g/L g/L g/L g/L g/L g/L ° C can

(

Incubate microaerobically at 30°C for 72 hours + 3 3 + hours 72 for 30°C at microaerobically Incubate NCM0190

cause the pH to fall outside of the specified pH pH the specified of outside fall pHto the cause

disease or other conditions in humans. conditions or other disease and

allow to solidify. Overlays may be used be may Overlays solidify. to allow

)

act as growth stimulants. growth as stimulants. act

Revision: 1 Effective Date: 5/13/2019 2. 1. Ref closed. tightly container by keeping and light moisture from Protect storage temperature. the same at environment humidity low in a container d Store Storage Procedures the of Limitations directed. as when stored container inintact its medium to applies Expiry the originalcolor. from changed has or appearance flowing free on the stamped date toexpiration Refer Expiration results. for references toappropriate Refer Results coli Escherichia lactis subsp. lactis sakei subsp. sakei Lactobacillus QC Minimum Appearance: Prepared Appearance Dehydrated Specifications Control Quality Sheet Specification Technical • • •

e enumeration of mesophilic lactic acid bacteria bacteria acid lactic mesophilic of enumeration stuffs feeding animal and food of Microbiology ISO 15214:1998 130 23, Bacteriol. Appl. Sharpe M and Rogosa, de J.C., Man, using acetic acid or sodium hydroxide is recommended. hydroxide or sodium acid acetic using +/5.7 limits pH specified the of outside fall to pH Occasionally, sterili Pediococci Although MRS Agar is optimi medium. variatio nutritional Due to rence ehydrated culture media culture ehydrated

:

WDCM 00013 (inhibited) WDCM may also occur. also may

z Prepared medium is a c medium Prepared ation of ation this of a medium : P - 135. owder is fine, slightly cohesive, light light slightly cohesive, fine, is owder n, some strains may be encountered that grow poorly or fail to grow on this this on grow to or fail poorly grow that be encountered strainsmay some n,

WDCM 00016 00016 WDCM at

WDCM 00015 WDCM z ed ed to be selective for Lactobacilli, some growth of

2

- 30 °C container. The dehydrated medium should be discarded if not not if discarded be should medium dehydrated The container.

, M.E. (1960). A medium for the cultivation of lactobacilli. J. lactobacilli. J. of the cultivation for medium A (1960). , M.E.

away from direct sunlight. direct sunlight. from away

t 121 lear, –

Colony count technique count technique Colony o C for 15 autoclaves,minutes, in some may cause the - beige

0.1. In these rare cases, adjustment of the medium the medium of adjustment cases, rare these In 0.1.

gel

.

beige – Once opened and recapped, place place and recapped, opened Once

Horizontal method for the for method Horizontal

powder with some lumps lumps with some powder at 30 at o C.

Leuconostoc spp

. and