Version: 1 Revision Date: 2015-03-27

Product Data Sheet Purified anti-Krox-20 (EGR2)

Catalog # / Size: 901101 / 100 µl Previously: Covance Catalog# PRB-236P Clone: Poly19011 Isotype: Rabbit Polyclonal Immunogen: This antibody was raised against 8-95 amino acids of Krox20 (also known as Egr2) fused to GST. Reactivity: Human, Mouse, Rat, Rodent, Chicken Preparation: The antibody was purified by affinity chromatography. Formulation: Phosphate-buffered solution + 0.03% Thimerosal. Concentration: 1 mg/ml Staining of Krox-20 (Egr2), clone Storage: Store at -20°C. Upon initial thawing, apportion into working aliquots and Poly19011, on formalin fixed store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing paraffin-embedded human colon at the antibody. For long-term storage, keep the antibody at -80°C. 10 µg/mL. Applications: Applications: IHC, WB Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • Western blot: 1:500* • IHC: 1:100

Application Notes: This antibody is effective in immunoblotting and immunohistochemistry (IHC) procedures in both whole mounts and in sections. *Predicted MW = 48 kD Application References: 1. Neeraja Syed, et al. Soluble Neuregulin-1 Has Bifunctional, Concentration-Dependent Effects on Schwann Cell Myelination J. Neurosci. (30):6122-6131, 2010 (WB) 2. Wakatsuki S, et al. Roles of Meltrin-α/ADAM19 in Progression of Schwann Cell Differentiation and Myelination during Sciatic Nerve Regeneration J. Biol. Chem. (284):2957-2966, 2009 (IHC) 3. Yu, Wei-Ming, et al. Schwann Cell-Specific Ablation of Laminin_1 Causes Apoptosis and Prevents Proliferation J. Neurosci. (25):4463-4472, 2005 (IHC) 4. Harris J, et al. Early Growth Response -2, a Zinc-Finger , Is Required for Full Induction of Clonal Anergy in CD4+ T Cells J Immunol (173):7331-7338, 2004 (WB) 5. Chavrier P, Lemain P, Revelad O, Bravo R, Charnay RP. Characterization of a mouse multigene family that encodes structures. Mol Cell Biol 8:1319-1326, 1988. 6. Chavrier P, Zerial M, Lemaire P, Almendral J, Bravo R, Charnay P. A gene encoding a with zinc fingers is activated during G0/G1 transition in cultured cells. EMBO J 7:29-35, 1988.

Other Names: Early growth response protein 2, EGR-2, Krox-20 homolog, Drosophila, zinc finger protein Krox-20, KROX-20, Drosophila, homolog (early growth response-2) Related Products:Product Clone Application Purified anti-FOX3 (NeuN) 1B7 IHC, WB, ICC Anti-HOX C8 C952-12E IHC, WB, IF Anti-Prox1 Poly19252 IHC, IF, WB Purified anti-Pax-2 Poly19010 IHC, WB, IP Purified anti-Pax-6 Poly19013 WB, IHC, IF Purified anti-Tubulin β 3 (TUBB3) TUJ1 IHC, WB, IP, ICC

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