TBPL1 Rabbit Pab

Leader in Biomolecular Solutions for Life Science TBPL1 Rabbit pAb Catalog No.: A7575 1 Publications Basic Information Background Catalog No. This gene encodes a member of the TATA box-binding protein family. TATA box-binding A7575 proteins play a critical role in transcription by RNA polymerase II as components of the transcription factor IID (TFIID) complex. The encoded protein does not bind to the TATA Observed MW box and initiates transcription from TATA-less promoters. This gene plays a critical role 21kDa in spermatogenesis, and single nucleotide polymorphisms in this gene may be associated with male infertility. Alternatively spliced transcript variants have been Calculated MW observed for this gene, and a pseudogene of this gene is located on the long arm of 20kDa chromosome 3. Category Primary antibody Applications WB, IF Cross-Reactivity Human, Mouse Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 9519 P62380 IF 1:50 - 1:200 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human TBPL1 (NP_004856.1). Synonyms TBPL1;MGC:8389;MGC:9620;STUD;TLF;TLP;TRF2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of 293T cells, using TBPL1 antibody (A7575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. Immunofluorescence analysis of A549 cells using TBPL1 antibody (A7575). Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
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WO 2019/079361 Al 25 April 2019 (25.04.2019) W 1P O PCT
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization I International Bureau (10) International Publication Number (43) International Publication Date WO 2019/079361 Al 25 April 2019 (25.04.2019) W 1P O PCT (51) International Patent Classification: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, C12Q 1/68 (2018.01) A61P 31/18 (2006.01) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, C12Q 1/70 (2006.01) HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (21) International Application Number: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, PCT/US2018/056167 OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (22) International Filing Date: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 16 October 2018 (16. 10.2018) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (30) Priority Data: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 62/573,025 16 October 2017 (16. 10.2017) US TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, ΓΕ , IS, IT, LT, LU, LV, (71) Applicant: MASSACHUSETTS INSTITUTE OF MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TECHNOLOGY [US/US]; 77 Massachusetts Avenue, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Cambridge, Massachusetts 02139 (US).
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Figure S1. HAEC ROS Production and ML090 NOX5-Inhibition
Figure S1. HAEC ROS production and ML090 NOX5-inhibition. (a) Extracellular H2O2 production in HAEC treated with ML090 at different concentrations and 24 h after being infected with GFP and NOX5-β adenoviruses (MOI 100). **p< 0.01, and ****p< 0.0001 vs control NOX5-β-infected cells (ML090, 0 nM). Results expressed as mean ± SEM. Fold increase vs GFP-infected cells with 0 nM of ML090. n= 6. (b) NOX5-β overexpression and DHE oxidation in HAEC. Representative images from three experiments are shown. Intracellular superoxide anion production of HAEC 24 h after infection with GFP and NOX5-β adenoviruses at different MOIs treated or not with ML090 (10 nM). MOI: Multiplicity of infection. Figure S2. Ontology analysis of HAEC infected with NOX5-β. Ontology analysis shows that the response to unfolded protein is the most relevant. Figure S3. UPR mRNA expression in heart of infarcted transgenic mice. n= 12-13. Results expressed as mean ± SEM. Table S1: Altered gene expression due to NOX5-β expression at 12 h (bold, highlighted in yellow). N12hvsG12h N18hvsG18h N24hvsG24h GeneName GeneDescription TranscriptID logFC p-value logFC p-value logFC p-value family with sequence similarity NM_052966 1.45 1.20E-17 2.44 3.27E-19 2.96 6.24E-21 FAM129A 129. member A DnaJ (Hsp40) homolog. NM_001130182 2.19 9.83E-20 2.94 2.90E-19 3.01 1.68E-19 DNAJA4 subfamily A. member 4 phorbol-12-myristate-13-acetate- NM_021127 0.93 1.84E-12 2.41 1.32E-17 2.69 1.43E-18 PMAIP1 induced protein 1 E2F7 E2F transcription factor 7 NM_203394 0.71 8.35E-11 2.20 2.21E-17 2.48 1.84E-18 DnaJ (Hsp40) homolog.
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TBP2 Is Essential for Germ Cell Development by Regulating Transcription and Chromatin Condensation in the Oocyte
Downloaded from genesdev.cshlp.org on September 24, 2021 - Published by Cold Spring Harbor Laboratory Press TBP2 is essential for germ cell development by regulating transcription and chromatin condensation in the oocyte Emese Gazdag,1,4 Ange`le Santenard,1,2,4 Ce´line Ziegler-Birling,1,2 Gioia Altobelli,1,3 Olivier Poch,3 La`szlo` Tora,1,5 and Maria-Elena Torres-Padilla1,2,6 1Department of Functional Genomics, Institut de Geńe´tique et de Biologie Molećulaire et Cellulaire (IGBMC), UMR 7104 CNRS, UdS, INSERM U964, BP 10142, F-67404 Illkirch Cedex, CU de Strasbourg, France; 2Department of Developmental and Cell Biology, Institut de Geńe´tique et de Biologie Molećulaire et Cellulaire (IGBMC), UMR 7104 CNRS, UdS, INSERM U964, BP 10142, F-67404 Illkirch Cedex, CU de Strasbourg, France; 3Bioinformatics and Integrative Biology Laboratory, Institut de Geńe´tique et de Biologie Molećulaire et Cellulaire (IGBMC), UMR 7104 CNRS, UdS, INSERM U964, BP 10142, F-67404 Illkirch Cedex, CU de Strasbourg, France Development of the germline requires consecutive differentiation events. Regulation of these has been associated with germ cell-specific and pluripotency-associated transcription factors, but the role of general transcription factors (GTFs) remains elusive. TATA-binding protein (TBP) is a GTF involved in transcription by all RNA polymerases. During ovarian folliculogenesis in mice the vertebrate-specific member of the TBP family, TBP2/ TRF3, is expressed exclusively in oocytes. To determine TBP2 function in vivo, we generated TBP2-deficient mice. We found that Tbp2À/À mice are viable with no apparent phenotype. However, females lacking TBP2 are sterile due to defective folliculogenesis, altered chromatin organization, and transcriptional misregulation of key oocyte-specific genes.
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Unraveling the Sperm Transcriptome by Next Generation Sequencing and the Global Epigenetic Landscape in Infertile Men Fadi Choucair
Unraveling the sperm transcriptome by next generation sequencing and the global epigenetic landscape in infertile men Fadi Choucair To cite this version: Fadi Choucair. Unraveling the sperm transcriptome by next generation sequencing and the global epigenetic landscape in infertile men. Molecular biology. Université Côte d’Azur; Université libanaise, 2018. English. NNT : 2018AZUR4058. tel-01958881 HAL Id: tel-01958881 https://tel.archives-ouvertes.fr/tel-01958881 Submitted on 18 Dec 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THÈSE DE DOCTORAT Exploration du transcriptome spermatique par le séquençage nouvelle génération et le portrait épigénétique de l’infertilité masculine Unraveling the sperm transcriptome by next generation sequencing and the global epigenetic landscape in infertile men Fadi CHOUCAIR INSERM U1065, C3M Présentée en vue de l’obtention Devant le jury, composé de : du grade de docteur en interactions Mme RACHEL LEVY, PR, UMRS 938, UPMC M. FABIEN MONGELARD, MC, CRCL, ENS Lyon moléculaires et cellulaires Mme NINA SAADALLAH-ZEIDAN,
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TRF2 and the Evolution of the Bilateria
Downloaded from genesdev.cshlp.org on September 26, 2021 - Published by Cold Spring Harbor Laboratory Press HYPOTHESIS TRF2 and the evolution of the bilateria Sascha H.C. Duttke,1 Russell F. Doolittle,1,2 Yuan-Liang Wang,1 and James T. Kadonaga1 1Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA; 2Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093, USA The development of a complex body plan requires a di- addition, TFIIA has been found to enhance the binding of versity of regulatory networks. Here we consider the TBP to DNA. TBP (as well as the TATA box), TFIIB, TFIIE, concept of TATA-box-binding protein (TBP) family pro- TFIIS, and Pol II are present in Archaea and eukaryotes teins as ‘‘system factors’’ that each supports a distinct set (for review, see Grohmann and Werner 2011). Moreover, of transcriptional programs. For instance, TBP activates many eukaryotes contain TBP as well as TBP-related TATA-box-dependent core promoters, whereas TBP-related factors (TRFs). factor 2 (TRF2) activates TATA-less core promoters that Here we consider the concept that TBP family proteins are dependent on a TCT or downstream core promoter function as ‘‘system factors’’ that support distinct transcrip- element (DPE) motif. These findings led us to investigate tional programs. TBP occurs in Archaea and eukaryotes, the evolution of TRF2. TBP occurs in Archaea and and TBP-related factor 1 (TRF1), TRF2, and TRF3 (for eukaryotes, but TRF2 evolved prior to the emergence reviews, see Goodrich and Tjian 2010; Akhtar and Veenstra of the bilateria and subsequent to the evolutionary split 2011) evolved independently via duplications of the between bilaterians and nonbilaterian animals.
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Discovering Key Transcriptomic Regulators in Pancreatic Ductal
bioRxiv preprint doi: https://doi.org/10.1101/2020.10.01.322768; this version posted October 2, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Discovering Key Transcriptomic Regulators in Pancreatic Ductal Adenocarcinoma using Dirichlet Process Gaussian Mixture Model Sk Md Mosaddek Hossain1,2,+, Aanzil Akram Halsana1, +, Lutfunnesa Khatun2, Sumanta Ray1, *, and Anirban Mukhopadhyay2, * 1Aliah University, Computer Science and Engineering, Kolkata, 700160, India 2University of Kalyani, Computer Science and Engineering, Kalyani, 741235, India *[email protected], [email protected] +these authors contributed equally to this work ABSTRACT Pancreatic Ductal Adenocarcinoma (PDAC) is the most lethal type of pancreatic cancer (PC), late detection of which leads to its therapeutic failure. This study aims to find out key regulatory genes and their impact on the progression of the disease helping the etiology of the disease which is still largely unknown. We leverage the landmark advantages of time-series gene expression data of this disease, and thereby the identified key regulators capture the characteristics of gene activity patterns in the progression of the cancer. We have identified the key modules and predicted gene functions of top genes from the compiled gene association network (GAN). Here, we have used the natural cubic spline regression model (splineTimeR) to identify differentially expressed genes (DEG) from the PDAC microarray time-series data downloaded from gene expression omnibus (GEO). First, we have identified key transcriptomic regulators (TR) and DNA binding transcription factors (DbTF).
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Analysis of Mir-30B As a Regulator of Tso45-G Anterograde Trafficking
Analysis of miR-30b as a Regulator of tsO45-G Anterograde Trafficking DISSERTATION submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences Sanchari Roy 2013 Dissertation submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by MSc in Molecular Biology Sanchari Roy born in Kolkata, India Oral-examination: 30.01.2014 Analysis of miR-30b as a Regulator of tsO45-G Anterograde Trafficking Referees: Prof. Dr. Walter Nickel Dr. Vytaute Starkuviene-Erfle To my Dear Parents ACKNOWLEDGEMENT First of all, I would like to express my sincere gratitude to my supervisor Dr. Vytaute Starkuviene-Erfle for giving me the opportunity to join her research group ‘Screening of Cellular Networks’, BioQuant, University of Heidelberg and successfully carry out my PhD work. Furthermore, I would like to thank for her guidance throughout four years of my project. I would like to thank Prof. Dr. Walter Nickel for being the first supervisor of my PhD studies and useful discussion during TAC meetings. I would also like to thank Prof. Dr. Stefan Wiemann and Prof. Dr. Ursula Kummer for agreeing to be the examiners for my doctoral thesis. I would sincerely like to thank Dr. Vladimir Kuryshev for sharing his exceptional expertise in bioinformatics with me and providing me confidence and motivation during the tenure of the project. I want to thank my lab colleagues Dr.
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Identification and Functional Characterisation of Non-Canonical DNA Methylation Readers in the Mammalian Brain
Identification and functional characterisation of non-canonical DNA methylation readers in the mammalian brain Sufyaan Mohamed School of Molecular Sciences ARC Centre of Excellence in Plant Energy Biology Harry Perkins Institute of Medical Research 2020 Supervisors: Professor Ryan Lister (60%) Associate Professor Ozren Bogdanovic (20%) Dr Yuliya Karpievitch (10%) Professor Ian Small (5%) Associate Professor Monika Murcha (5%) i i. Thesis Declaration: I, Sufyaan Mohamed, certify that: The work in this thesis has been substantially accomplished during enrolment in this degree. This thesis does not contain material which has been accepted for the reward of any other degree or diploma in my name, in any university or other tertiary institution. No part of this work will, in the future, be used in a submission in my name, for any other degree or diploma in any university or other tertiary institution without the prior approval of The University of Western Australia and where applicable, any partner institution responsible for the joint award of this degree. This thesis does not contain any material previously published or written by another person, except where due reference has been made in the text. The work(s) presented here are not in any way a violation or infringement of any copyright, trademark, patent, or other rights whatsoever of any person. Mass spectrometry samples were processed by Ino Karemaker. The ProteoMM code used for the analysis of the human and mouse datasets described in Chapter III was written by Dr. Yuliya Karpievitch This thesis contains published work and/or work prepared for publication, some of which has been co-authored.
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Mirna Clusters with Up-Regulated Expression in Colorectal Cancer
cancers Review miRNA Clusters with Up-Regulated Expression in Colorectal Cancer Paulína Pidíková and Iveta Herichová * Department of Animal Physiology and Ethology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkoviˇcova6, 842 15 Bratislava, Slovakia; [email protected] * Correspondence: [email protected]; Tel.: +421-602-96-572 Simple Summary: As miRNAs show the capacity to be used as CRC biomarkers, we analysed exper- imentally validated data about frequently up-regulated miRNA clusters in CRC tissue. We identiﬁed 15 clusters that showed increased expression in CRC: miR-106a/363, miR-106b/93/25, miR-17/92a-1, miR-181a-1/181b-1, miR-181a-2/181b-2, miR-181c/181d, miR-183/96/182, miR-191/425, miR-200c/141, miR-203a/203b, miR-222/221, mir-23a/27a/24-2, mir-29b-1/29a, mir-301b/130b and mir-452/224. Cluster positions in the genome are intronic or intergenic. Most clusters are regu- lated by several transcription factors, and by long non-coding RNAs. In some cases, co-expression of miRNA with other cluster members or host gene has been proven. miRNA expression patterns in cancer tissue, blood and faeces were compared. The members of the selected clusters target 181 genes. Their functions and corresponding pathways were revealed with the use of Panther analysis. Clusters miR-17/92a-1, miR-106a/363, miR-106b/93/25 and miR-183/96/182 showed the strongest association with metastasis occurrence and poor patient survival, implicating them as the most promising targets of translational research. Abstract: Colorectal cancer (CRC) is one of the most common malignancies in Europe and North Citation: Pidíková, P.; Herichová, I.
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Discerning the Role of Foxa1 in Mammary Gland
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Thesis Reference
Thesis EZH2 induces methylation of ERG enhancing its transcriptional and transforming activity CURTI, Laura Abstract The TMPRSS2-ERG gene fusion is one of the most frequent gene rearrangements found in about 50% of human prostate tumors. However, uncertainty persists regarding the molecular determinants of ERG-induced oncogenesis and its role in tumor progression. We report a novel molecular interaction between ERG and the H3K27 histone methyltransferase EZH2. We found that ERG possesses a H3K27-like motif (RKS) centered at lysine 362 (K362), and that EZH2 was able to catalyze its methylation. K362 methylation, and interaction with EZH2, enhanced both trans-activation and trans-repression mediated by ERG and contributed to global transcriptional and phenotypic reprograming in ERG positive tumors. ERG and EZH2 co-localized in several regions of the genome of ERG positive prostate cancer cells, and most of these co-occupied genes constituted a set of highly deregulated genes in primary and metastatic ERG positive tumors. Notably, PTEN loss, which is frequently observed in prostate tumors with ERG fusion, and is associated with adverse prognosis, promoted ERG methylation. This study gives new insights providing a broadly relevant mechanism [...] Reference CURTI, Laura. EZH2 induces methylation of ERG enhancing its transcriptional and transforming activity. Thèse de doctorat : Univ. Genève, 2016, no. Sc. 4913 URN : urn:nbn:ch:unige-882629 DOI : 10.13097/archive-ouverte/unige:88262 Available at: http://archive-ouverte.unige.ch/unige:88262
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