Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations
2010 Global patterns of Mutator transposon insertions and high-throughput strategies for gene mapping and cloning in maize Sanzhen Liu Iowa State University
Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Cell and Developmental Biology Commons, and the Genetics and Genomics Commons
Recommended Citation Liu, Sanzhen, "Global patterns of Mutator transposon insertions and high-throughput strategies for gene mapping and cloning in maize" (2010). Graduate Theses and Dissertations. 11886. https://lib.dr.iastate.edu/etd/11886
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( ' #)#!.( (45 (") $)$'% !. #) . #) "' '( ! # )$ #$" ' $# (($ ) , ) ) (!) % #$).%0 , ,( '() (' . !"$'24 7==752 #+$!+( #&*#) . # #) ( ' ) # "' '( )$)'" # '#(),#),$%$$! ("%!(' + '$"( ' ) # %$%*!) $#2 $''(( +"*)#)0) "*)#)%$$!$#) #( # + *!($ "*)#)% #$).%0, !) #$#3 "*)#) %$$! $#) #( # + *!( , ) $*) "*)#) % #$).%2 ) (* #) %$$! ( /0 ) ),$ '(*!)#)*! ("%!( ' #) !!.$#!. #) #$" ' $#((($ ), ) "*)#) % #$).%2*(&*#)!.0) ! # ),#"' '(#) )' ) # ( "(*' + &*#) ) $#$ #) "' '(2 #.).%($ #) "' '( +#*( $'0 #!* # .' /) $#3( %%'$ ( (* ( '()' ) $# ' "#) !# ) %$!."$'% ("( 4 (5 4 7==750( # ! )*'%$!."$'% ("454 866:50# +'( ).''. 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' &" "" "$! &%2 %%!'$ "" $! &2 32! .- & +%6446-! .- & +%64463- !" &2 #' .- &%64493--/ 1 2 ":32' "' %3- 2 ' .- &%645433.! $ $%)$'%&! !&+"&! %%"$& !&%%&'+.% !)$&"$!(% &+"% ' $%! $ $%'%&! !&+" %. %! %'"" & '$: 7-8. 110 111 2,101 Figure S2 2000 1600 1,231 1200 per Mb Mu 800 Number of 391 400 262 216 174 215 71 0 1st Exons Introns -100 bp 5’ UTR 1st Introns Internal ExonsLast Exons 3’ UTRs (no UTR) (2nd~last) 112 113 Figure S4 chr 1 chr 2 chr 3 chr 4 chr 5 2.5 cM per Mb 0.5 1.5 2.5 01234 0.0 1.0 2.0 3.0 0.0 0.5 1.0 1.5 2.0 2.5 0.0 0.5 1.0 1.5 2.0 0 50 100 200 300 0 50 100 150 200 0 50 100 150 200 0 50 100 150 200 250 0 50 100 150 200 chr 6 chr 7 chr 8 chr 9 chr 10 45 34 2 23 cM per Mb 1 0 01234 01 0.0 1.0 2.0 3.0 0.0 1.0 2.0 3.0 0 50 100 150 0 50 100 150 0 50 100 150 0 50 100 150 0 50 100 150 Mb Mb Mb Mb Mb 114 Figure S5 chr 2 chr 3 chr 4 2 −1 0 1 2 −1012 −1 0 1 0 50 100 150 200 0 50 100 150 200 0 50 100 150 200 250 chr 5 chr 6 chr 7 −1012 −1012 −1 0 1 2 0 50 100 150 200 0 50 100 150 0 50 100 150 chr 8 chr 9 chr 10 2 1 −1 0 1 2 −1 0 −1012 0 50 100 150 0 50 100 150 0 50 100 150 Mb Mb Mb 115 Figure S6 chr 2 chr 3 chr 4 −1012 −1012 −1012 0 50 100 150 200 0 50 100 150 200 0 50 100 150 200 250 chr 5 chr 6 chr 7 2 1 0 −1 0 1 2 −1 −1 0 1 2 0 50 100 150 200 0 50 100 150 0 50 100 150 chr 8 chr 9 chr 10 2 1 0 −1 0 1 2 −1 −1 0 1 2 0 50 100 150 0 50 100 150 0 50 100 150 Mb Mb Mb 116 Figure S7 ISU Integrated Map (357 RILs) 3 Genoplante Map (91 RILs) ISU Map7 (91 RILs) 0 0 00 00 000 0 0 4 0 MMP Map (302 RILs) MMP ISU SNP_v1 Map (291 RILs) 87 00 0 0 00 59 52 152 ISU Integrated Map (357 RILs, this study) ISU Map7 (91 RILs, unpublished) ISU SNP_v1 Map (291 RILs, Liu et al., Genetics 2010) Genoplante Map (91 RILs, Falque et al., Genetics 2005) Missouri Mapping Project (MMP) Map (302 RILs, Coe et al., Plant Phys 2002, Cone et al., Plant Phys 2002) 117 118 119 120 121 !%$ ! 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