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Double IPA® and Sutterpatch® Are Trademarks of Sutter Instrument Company

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® / IPA ® DOUBLE IPA Integrated Patch Amplifier ELECTROPHYSIOLOGY PATCH-CLAMP SYSTEM WITH SutterPatch® SOFTWARE Operation Manual One Digital Drive Novato, CA 94949 USA Voice: +1 415-883-0128 Web: www.sutter.com Fax: +1 415-883-0572 Email: [email protected] 2 The IPA and Double IPA systems have two international safety certifications: 1) The CE mark is for compliance to health, safety and environmental protection standards for products sold within the European Economic Area: 2) The RoHS (Restriction of Hazardous Substances) Directive 2002/95/EC restricts the use of hazardous substances for electronic equipment sold within the European Union: Copyright © 2015 - 2021 Sutter Instrument Company. All Rights Reserved. IPA®, Double IPA® and SutterPatch® are trademarks of Sutter Instrument Company. All other product and company names are trademarks or registered trademarks of their respec- tive holders. INTEGRATED PATCH AMPLIFIER – OPERATION MANUAL – REV. 2.1.0 (2021-1) 3 DISCLAIMER The IPA system consists of one electronic amplifier with integrated digitizer and one headstage. The Double IPA system consists of one electronic amplifier with integrated digitizer and two headstages. All references to an IPA system also include a Double IPA system, unless otherwise noted. The purpose of the system is for the stimulation and measurement of cellular prepara- tions. No other use is recommended. This instrument is designed for use in a laboratory environment. It is not intended for, nor should it be used in human experimentation or applied to humans in any way. This is not a medical device. Do not open or attempt to repair the instrument. Do not allow an unauthorized and/or untrained operative to use this instrument. Any misuse will be the sole responsibility of the user/owner, and Sutter Instrument Company assumes no implied or inferred liability for direct or consequential damages from this instru- ment if it is operated or used in any way other than for which it is designed. SAFETY WARNINGS AND PRECAUTIONS Electrical ▪ Operate the IPA system using 100 – 240 VAC, 50 - 60 Hz line voltage. This instru- ment is designed for use in a laboratory environment that has low electromagnet- ic noise and mechanical vibration. Surge suppression is recommended at all times. Fuse Replacement: Replace only with the same type and rating: Line Voltage: 100 – 240 VAC Manufacturer Examples Fuse RoHS Compliant (Lead Free) Rating ▪ Bussmann: GMC-2-R, S506-2A T2.0, Littelfuse: 239.002.P 250V IPA & DIPA Fuses Type: 5 x 20 mm glass tube, Medium Time Delay (Slow Blow), RoHS compliant. Rating: T2.0A 250V (Time Delay, 2 Amps, 250 Volts) Examples: Bussmann: GMC-2-R, S506-2A Littelfuse: 239.002.P INTEGRATED PATCH AMPLIFIER – OPERATION MANUAL – REV. 2.1.0 (2021-1) 4 ▪ Avoiding Electrical Shock and Fire-related Injury ▪ Always use the grounded power cord provided to connect the system’s power adapter to a grounded/earthed mains outlet. This is required to pro- tect you from injury in the event that an electrical hazard occurs. ▪ Do not disassemble the system. Refer servicing to qualified personnel. ▪ To prevent fire or shock hazard, do not expose the unit to rain or moisture. Operational Failure to comply with any of the following precautions may damage this instrument. ▪ This instrument is designed for operation in a laboratory environment (Pollution Degree I) that is free from mechanical vibrations, electrical noise and transients. ▪ Operate this instrument only according to the instructions included in this man- ual. ▪ Do not operate this instrument near flammable materials. The use of any hazardous materials with this instrument is not recommended and, if un- dertaken, is done so at the users’ own risk. ▪ Do not operate if there is any obvious damage to any part of the instru- ment. Other ▪ Retain the original packaging for future transport of the instrument. ▪ Sutter Instrument Company reserves the right to change specifications without prior notice. ▪ Use of this instrument is for research purposes only. INTEGRATED PATCH AMPLIFIER – OPERATION MANUAL – REV. 2.1.0 (2021-1) 5 Handling Micropipettes Failure to comply with any of the following precautions may result in injury to the users of this instrument as well as those working in the general area near the instrument. ▪ The micropipettes used with this instrument are very sharp and relatively fragile. Avoid contact with micropipette tips to prevent accidentally impaling yourself. ▪ Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps” container. INTEGRATED PATCH AMPLIFIER – OPERATION MANUAL – REV. 2.1.0 (2021-1) 6 TABLE OF CONTENTS DISCLAIMER ........................................................................................................................ 3 SAFETY WARNINGS AND PRECAUTIONS ................................................................ 3 Electrical ............................................................................................................................................................................. 3 Operational ........................................................................................................................................................................ 4 Other ...................................................................................................................................................................................... 4 Handling Micropipettes ........................................................................................................................................... 5 1. INTRODUCTION ........................................................................................................... 14 1.1 Overview .................................................................................................................................................................... 14 1.2 Software Highlights .......................................................................................................................................... 14 1.3 Experiment Structure ..................................................................................................................................... 15 2. INSTALLATION ............................................................................................................. 19 2.1 Computer Requirements ............................................................................................................................... 19 2.2 SutterPatch System Environment ......................................................................................................... 20 2.3 Mounting Instructions .................................................................................................................................... 21 2.4 Electrical Connections .................................................................................................................................... 21 2.5 Install Hardware ................................................................................................................................................. 21 2.6 Install Software .................................................................................................................................................... 22 2.7 Test System .................................................................................................................................................................. 23 2.7.1 Install Model Cell ............................................................................................................................................................. 23 2.7.2 Startup ..................................................................................................................................................................................... 24 2.7.3 Run a Membrane Test .................................................................................................................................................. 28 3. HARDWARE OPERATION ......................................................................................... 30 3.1 IPA Front Panel ................................................................................................................................................... 30 3.2 Double IPA Front Panel ................................................................................................................................. 32 3.3 IPA Rear Panel...................................................................................................................................................... 32 3.4 Grounding ................................................................................................................................................................ 33 3.5 Headstage ................................................................................................................................................................. 33 3.6 Holder .......................................................................................................................................................................... 34 3.6.1 Assembly ................................................................................................................................................................................. 35 3.6.2 Chloriding Silver Wire .................................................................................................................................................
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