ELECTROPHORESIS

Supporting Information for Electrophoresis DOI 10.1002/elps.200305809

Jae-Won Yang, Thomas Czech and Gert Lubec

Proteomic profiling of human hippocampus

ª 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Jae-Won Yang 16

Table 2. Proteins identified in the human hippocampus

Identification Number of Number Entry name Protein name (Synonym) Mr identified Matches Scoreb spot proteins 1QNMAa Manganese superoxide dismutase (EC 1.15.1.1) 22290 7 68 1 mutant Q143N, chain A - Human O00217 NUIM_HUMAN NADH-ubiquinone 23 kDa subunit, 24203 9 81 1 mitochondrial [Precursor] (EC 1.6.5.3) (EC .6.99.3) (Complex I-23KD) (CI-23KD) (TYKY subunit) P00352* DHA1_HUMAN Aldehyde dehydrogenase 1A1 (EC 1.2.1.3.) 55323 15 118 1 (Aldehyde dehydrogenase, cytosolic) (ALDH class 1) (ALHDII) (ALDH-E1) P05091* DHAM_HUMAN Aldehyde dehydrogenase, mitochondrial precursor 56859 23 196 1 (EC 1.2.1.3) (ALDH class 2) (ALDHI) (ALDH-E2). P10599 THIO_HUMAN Thioredoxin (ATL-derived factor) (ADF) (Surface 11884 10 120 1 associated sulphydryl protein) (SASP) P28161 GTM2_HUMAN Glutathione S- Mu 2 (EC 2.5.1.18) 25768 10 107 1 (GSTM2-2) (GST class-mu 2) P30041 PDX6_HUMAN Peroxiredoxin 6 (EC 1.11.1.-) (Antioxidant protein 2) 25002 13 130 2 (1-Cys peroxiredoxin) (1-Cys PRX) (Acidic calcium- independent phospholipase A2) (EC 3.1.1.-) (aiPLA2) (Non-selenium glutathione peroxidase) (EC 1.11.1.7) (NSGPx) (24 kDa protein) (Liver 2D page spot 40) (Red blood cells page spot 12) P30048 PDX3_HUMAN Thioredoxin-dependent peroxide reductase, 28017 9 87 2 mitochondrial [Precursor] (EC 1.11.1.-) (Peroxiredoxin 3) (Antioxidant protein 1) (AOP-1) (MER5 protein homolog) (HBC189) (PRX III) P31040 DHSA_HUMAN Succinate dehydrogenase [ubiquinone] flavoprotein 73670 16 173 3 subunit mitochondrial [precursor] (EC 1.3.5.1) (Fp) (Flavoprotein subunit of complex II) P32119 PDX2_HUMAN Peroxiredoxin 2 (EC 1.11.1.-) (Thioredoxin 18486 7 91 1 peroxidase 1) (Thioredoxin-dependent peroxide reductase 1) (-specific antioxidant protein) (TSA) (PRP) (Natural killer enhancing factor B) (NKEF-B) P47985 UCRI_HUMAN Ubiquinol-cytochrome C reductase iron-sulfur 29918 9 80 2 subunit, mitochondrial [Precursor] (EC 1.10.2.2) (Rieske iron-sulfur protein) (RISP) P48637 GSHB_HUMAN Glutathione synthetase (EC 6.3.2.3) (Glutathione 52523 19 155 1 synthase) (GSH synthetase) (GSH-S) P49419 D7A1_HUMAN Aldehyde dehydrogenase family 7 member A1 (EC 55845 17 117 2 1.2.1.3) (Antiquitin 1) P78417 GTO1_HUMAN Glutathione transferase omega 1 (EC 2.5.1.18) (GSTO 27833 9 67 2 1-1) Chaperones P02511 CRAB_HUMAN Alpha crystallin B chain (Alpha(B)-crystallin) 20146 10 116 3 (Rosenthal fiber component) P05092 CYPH_HUMAN Peptidyl-prolyl cis-trans A (EC 5.2.1.8) 18098 9 113 3 (PPIase) (Rotamase) (Cyclophilin A) (Cyclosporin A- binding protein) P08107 HS71_HUMAN Heat shock 70 kDa protein 1 (HSP70.1) (HSP70- 70294 27 226 2 1/HSP70-2). P17987 TCPA_HUMAN T-complex protein 1, alpha subunit (TCP-1-alpha) 60869 20 184 2 (CCT-alpha) P30101 PDA3_HUMAN Protein isomerase A3 [Precursor] (EC 57147 24 190 2 5.3.4.1) (Disulfide isomerase ER-60) (ERp60) (58 kDa microsomal protein) (p58) (ERp57) (58 kDa glucose regulated protein) P31948 IEFS_HUMAN Stress-induced-phosphoprotein 1 (STI1) 63227 18 108 1 (Hsp70/Hsp90-organizing protein) (Transformation- sensitive protein IEF SSP 3521) P35232 PHB_HUMAN Prohibitin 29843 12 136 1 Jae-Won Yang 17

Identification Number of Number Entry name Protein name (Synonym) Mr identified Matches Scoreb spot P38646 GR75_HUMAN Stress-70 protein, mitochondrial [Precursor] (75 kDa 73967 25 256 1 glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (Mortalin) (MOT) P40227 TCPZ_HUMAN T-complex protein 1, zeta subunit (TCP-1-zeta) (CCT- 58444 16 129 2 zeta) (CCT-zeta-1) (Tcp20) (HTR3) P48643 TCPE_HUMAN T-complex protein 1, epsilon subunit (TCP-1-epsilon) 60089 33 221 2 (CCT-epsilon) P49368 TCPG_HUMAN T-complex protein 1, gamma subunit (TCP-1-gamma) 60862 20 168 1 (CCT-gamma) P54652 HS72_HUMAN Heat shock-related 70 kDa protein 2 (Heat shock 70 70263 12 107 1 kDa protein 2) P78371 TCPB_HUMAN T-complex protein 1, beta subunit (TCP-1-beta) 57794 38 344 2 (CCT-beta) Q12931 TRAL_HUMAN Heat shock protein 75 kDa, mitochondrial [Precursor] 80345 11 75 1 (HSP 75) (Tumor necrosis factor type 1 receptor associated protein) (TRAP-1) (TNFR-associated protein 1) Q9P0G7 Q9P0G7 Lambda-crystallin 33798 10 68 1

Cytoskeleton proteins O75083* WDR1_HUMAN WD-repeat protein 1 (Actin interacting protein 1) 66822 14 113 2 (AIP1) (NORI-1) P02571 ACTG_HUMAN Actin, cytoplasmic 2 (Gamma-actin) 26147 12 94 1 P04350 TBBX_HUMAN Tubulin beta-5 chain (Tubulin 5 beta) 50055 14 85 1 P05209 TBA1_HUMAN Tubulin alpha-1 chain (Alpha-tubulin 1) 50810 19 149 5 P06396 GELS_HUMAN Gelsolin precursor, plasma (Actin-depolymerizing 86043 10 81 2 factor) (ADF) (Brevin) (AGEL) P15311 EZRI_HUMAN Ezrin (p81) (Cytovillin) (Villin 2) 69339 17 120 2 P18206 VINC_HUMAN Vinculin (Metavinculin) 117088 9 65 1 P18282 DEST_HUMAN Destrin (Actin-depolymerizing factor) (ADF) 18950 9 83 1 P23528 COF1_HUMAN Cofilin, non-muscle isoform (18 kDa phosphoprotein) 18719 11 174 1 (P18) P26038 MOES_HUMAN Moesin (Membrane-organizing extension spike 67761 13 88 2 protein) P31146 CO1A_HUMAN Coronin-like protein p57 (Coronin 1A) 51678 11 118 1 P35080 PRO2_HUMAN Profilin II 15378 6 78 1 P42024 ACTZ_HUMAN Alpha-centractin (Centractin) (Centrosome-associated 42701 19 154 1 actin homolog) (Actin-RPV) (ARP1) P47755* CAZ2_HUMAN F-actin capping protein alpha-2 subunit (CapZ alpha- 33157 7 66 1 2) Q13841 Q13841 Beta-centractin [Fragment] 37187 15 117 1 Q13885 Q13885 Beta tubulin (Tubulin, beta polypeptide) 50274 23 162 5 (DJ40E16.7.1) (Novel protein similar to tubulin beta polypeptide (TUBB), variant 1) Q16658 FSC1_HUMAN Fascin (Singed-like protein) (55 kDa actin bundling 54992 7 73 1 protein) (p55) Q16891 IMMT_HUMAN Mitochondrial inner membrane protein (Mitofilin) 84026 20 159 3 (p87/89) Q923G3 Q923G3 Capping protein (Actin filament) muscle Z-line, beta 30952 11 70 1 Q92599 Y202_HUMAN Septin-like protein KIAA0202 [Fragment] 58318 15 154 1 Q96HG5* Q96HG5 Actin, beta [Fragment] 41321 9 64 1 Q9BPX5 Q9BPX5 Similar to actin related protein 2/3 complex, subunit 5 16931 7 83 1 (16 kD) Q9BQE3 TBA6_HUMAN Tubulin alpha-6 chain (Alpha-tubulin 6) 50548 11 80 1 Q9NZR1 TMO2_HUMAN Neuronal tropomodulin (N-Tmod) (Tropomodulin 2) 39571 16 89 1

Hypothetical proteins Q96DD2 Q96DD2 Hypothetical protein 52.6 kDa protein 53297 27 229 2 Q96GX7 Q96GX7 Similar to selenium binding protein 1 52928 18 160 1 Q96NQ1 Q96NQ1 Hypothetical protein FLJ30356 26738 7 66 1 Q9BTD2 Q9BTD2 Hypothetical protein [Fragment] 41506 14 106 2 Q9H0R4 Q9H0R4 Hypothetical protein 28746 11 107 1 Q9H0W9 Q9H0W9 Hypothetical protein 35608 6 66 1 Jae-Won Yang 18

Identification Number of Number Entry name Protein name (Synonym) Mr identified Matches Scoreb spot Q9HC37 Q9HC37 Hypothetical protein 33570 13 102 1 Q9NTK5 PTD4_HUMAN Putative GTP-binding protein PTD004 (PRO2455). 44943 16 199 1 Q9NVA2 Q9NVA2 Hypothetical protein FLJ10849 49652 16 181 3 Q9P042 Q9P042 HSPC108 37293 8 62 1 Q9UF24 Q9UF24 Hypothetical protein [Fragment] (EC 1.6.5.3) (NADH 22318 16 198 1 dehydrogenase) Q9UG16 Q9UG16 Hypothetical protein DKFZP564P0562 [Fragment] 152451 23 133 2 Q9UGY2 Q9UGY2 DJ37E16.5 (Novel protein similar to 32077 12 102 1 NITROPHENYLPHOSPHATASES from VARIOUS ORGANISMS) (Hypothetical protein) Q9Y6E9 Q9Y6E9 type 2 43782 13 131 1

Metabolic proteins O00154 BACH_HUMAN Cytosolic acyl coenzyme A thioester (EC 37795 20 179 4 3.1.2.2) (Long chain acyl-CoA thioester hydrolase) (CTE-II) (Brain acyl-CoA hydrolase) O43175* SERA_HUMAN D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) 57356 15 141 2 (3-PGDH) O75947 ATPQ_HUMAN ATP synthase D chain, mitochondrial (EC 3.6.3.14) 18405 11 124 1 (My032 protein) O94760 DDH1_HUMAN NG,NG-dimethylarginine dimethylaminohydrolase 1 31313 15 169 1 (EC 3.5.3.18) (Dimethylargininase 1) (Dimethylarginine dimethylaminohydrolase 1) (DDAHI) O95336 6PGL_HUMAN 6-phosphogluconolactonase (EC 3.1.1.31) (6PGL) 27815 9 104 1 P00338 LDHA_HUMAN L-lactate dehydrogenase A chain (EC 1.1.1.27) (LDH- 36819 16 150 1 A) (LDH muscle subunit) (LDH-M) P00938 TPIS_HUMAN Triosephosphate isomerase (EC 5.3.1.1) (TIM) 26807 12 142 2 P04406 G3P2_HUMAN Glyceraldehyde 3-phosphate dehydrogenase, liver (EC 36202 18 131 8 1.2.1.12) (GAPDH) P06733 ENOA_HUMAN Alpha enolase (EC 4.2.1.11) (2-phospho-D-glycerate 47350 14 91 3 hydro-) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase) P07195 LDHB_HUMAN L-lactate dehydrogenase B chain (EC 1.1.1.27) (LDH- 36900 11 62 1 B) (LDH heart subunit) (LDH-H) P07954 FUMH_HUMAN Fumarate hydratase, mitochondrial [Precursor] (EC 54773 16 98 2 4.2.1.2) (Fumarase) P08559 ODPA_HUMAN Pyruvate dehydrogenase E1 component alpha subunit, 43952 9 61 1 somatic form, mitochondrial precursor (EC 1.2.4.1) (PDHE1-A type I) P09329 KPR1_HUMAN Ribose-phosphate pyrophosphokinase I (EC 2.7.6.1) 35194 13 126 1 (Phosphoribosyl pyrophosphate synthetase I) (PPRibP) (PRS-I) P09382 LEG1_HUMAN Galectin-1 (Beta-galactoside-binding lectin L-14-I) 14917 8 94 1 (Lactose-binding lectin 1) (S-Lac lectin 1) (Galaptin) (14 kDa lectin) (HPL) (HBL) P09622 DLDH_HUMAN Dihydrolipoamide dehydrogenase, mitochondrial 54812 13 137 1 [Precursor] (EC 1.8.1.4) (Glycine cleavage system L protein) P09972* ALFC_HUMAN Fructose-bisphosphate aldolase C (EC 4.1.2.13) 39699 22 242 2 (Brain-type aldolase) P11182 ODB2_HUMAN Lipoamide acyltransferase component of branched- 53324 15 96 1 chain alpha-keto acid dehydrogenase complex, mitochondrial [Precursor] (EC 2.3.1.-) (E2) (Dihydrolipoamide branched chain transacylase) (BCKAD E2 subunit) P11498 PYC_HUMAN Pyruvate carboxylase, mitochondrial [Precursor] (EC 130298 36 304 1 6.4.1.1) (Pyruvic carboxylase) (PCB) P11586 C1TC_HUMAN C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF 102049 11 74 1 synthase) P12277 KCRB_HUMAN Creatine kinase, B chain (EC 2.7.3.2) (B-CK) 42876 21 217 3 P12532 KCRU_HUMAN Creatine kinase, ubiquitous mitochondrial precursor 47406 22 210 1 (EC 2.7.3.2) (U-MtCK) (Mia-CK) (Acidic-type Jae-Won Yang 19

Identification Number of Number Entry name Protein name (Synonym) Mr identified Matches Scoreb spot mitochondrial creatine kinase). P13716 HEM2_HUMAN Delta-aminolevulinic acid dehydratase (EC 4.2.1.24) 36758 6 63 1 (Porphobilinogen synthase) (ALADH) P14618 KPY1_HUMAN Pyruvate kinase, M1 isozyme (EC 2.7.1.40) Pyruvate 58280 12 149 1 kinase muscle isozyme) (Cytosolic thyroid hormone- binding protein) (CTHBP) (THBP1) P15104 GLNA_HUMAN Glutamine synthetase (EC 6.3.1.2) (Glutamate-- 42665 15 107 1 ammonia ) P17174 AATC_HUMAN Aspartate aminotransferase, cytoplasmic (EC2.6.1.1) 46316 19 139 2 (Transaminase A) (Glutamate oxaloacetate transaminase-1) P18669 PMG1_HUMAN Phosphoglycerate mutase 1 (EC 5.4.2.1) (EC 5.4.2.4) 28769 19 209 4 (EC 3.1.3.13) (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1) P21281 VAB2_HUMAN Vacuolar ATP synthase subunit B, brain isoform (EC 55708 28 236 2 3.6.3.14) (V-ATPase B2 subunit) (Vacuolar proton pump B isoform 2) (Endomembrane proton pump 58 kDa subunit) (HO57) P21695 GPDA_HUMAN Glycerol-3-phosphate dehydrogenase [NAD+], 38066 10 87 1 cytoplasmic (EC 1.1.1.8) (GPD-C) (GPDH-C) P23381 SYW_HUMAN Tryptophanyl-tRNA synthetase (EC 6.1.1.2) 53478 18 147 1 (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) P28838 AMPL_HUMAN Cytosol aminopeptidase (EC 3.4.11.1 53006 16 155 1 (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase) (Proline aminopeptidase) (EC 3.4.11.5) (Prolyl aminopeptidase) P30042 ES1_HUMAN ES1 protein homolog, mitochondrial precursor 28495 11 99 2 (Protein KNP-I) (GT335 protein) P30084 ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial [Precursor] (EC 31807 12 130 1 4.2.1.17) (Short chain enoyl-CoA hydratase) (SCEH) (Enoyl-CoA hydratase 1) P34949 MANA_HUMAN Mannose-6-phosphate isomerase (EC 5.3.1.8) 47065 20 140 1 (Phosphomannose isomerase) (PMI) (Phosphohexomutase) P35998 PRS7_HUMAN 26S protease regulatory subunit 7 (MSS1 protein) 49002 10 62 1 P36543 VATE_HUMAN Vacuolar ATP synthase subunit E (EC 3.6.3.14) (V- 26186 8 72 2 ATPase E subunit) (Vacuolar proton pump E subunit) (V-ATPase 31 kDa subunit) (P31). P40925 MDHC_HUMAN Malate dehydrogenase, cytoplasmic (EC 1.1.1.37) 36500 15 160 2 P41250* SYG_HUMAN Glycyl-tRNA synthetase (EC 6.1.1.14) (Glycine-- 78148 21 154 1 tRNA ligase) (GlyRS) P45381 ACY2_HUMAN (EC 3.5.1.15) (Aminoacylase-2) 36225 11 95 1 (ACY-2) P48163 MAOX_HUMAN NADP-dependent malic (EC 1.1.1.40) 63859 20 146 1 (NADP-ME) (Malic enzyme 1) P48735 IDHP_HUMAN Isocitrate dehydrogenase [NADP], mitochondrial 51333 12 70 1 [Precursor] (EC 1.1.1.42) (Oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) (ICD-M) P49591 SYS_HUMAN Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--tRNA 59122 20 184 1 ligase) (SerRS) P50213* IDHA_HUMAN Isocitrate dehydrogenase [NAD] subunit alpha, 40022 12 102 2 mitochondrial precursor (EC 1.1.1.41) (Isocitric dehydrogenase) (NAD+-specific ICDH) P55809* SCOT_HUMAN Succinyl-CoA:3-ketoacid-coenzyme A transferase, 56578 14 105 3 mitochondrial [Precursor] (EC 2.8.3.5) (Succinyl CoA:3-oxoacid CoA-transferase) Q01469 FABE_HUMAN Fatty acid-binding protein, epidermal (E-FABP) 15295 7 63 1 (Psoriasis-associated fatty acid-binding protein homolog) (PA-FABP) Q14697 Q14697 Glucosidase II [Precursor] (Hypothetical protein 107158 24 171 2 KIAA0088) Q15181 IPYR_HUMAN Inorganic pyrophosphatase (EC 3.6.1.1) 32277 8 62 1 Jae-Won Yang 20

Identification Number of Number Entry name Protein name (Synonym) Mr identified Matches Scoreb spot (Pyrophosphate phospho-hydrolase) (PPase) Q16775 GLO2_HUMAN Hydroxyacylglutathione hydrolase (EC 3.1.2.6) 29184 9 75 1 (Glyoxalase II) (GLX II) Q99798 ACON_HUMAN Aconitate hydratase, mitochondrial precursor (EC 86113 22 173 2 4.2.1.3) (Citrate hydro-lyase) (Aconitase) Q9HAP9 Q9HAP9 Glycerol-3-phosphate dehydrogenase 3 67850 25 233 2 Q9P2R7 SCB1_HUMAN Succinyl-CoA ligase [ADP-forming] beta-chain, 46732 18 89 2 mitochondrial [Precursor] (EC 6.2.1.5) (Succinyl-CoA synthetase, betaA chain) (SCS-betaA) (ATP-specific succinyl-CoA synthetase beta subunit) Q9Y2T3 GUAD_HUMAN (EC 3.5.4.3) (Guanase) (Guanine 51484 22 184 1 aminase) (Guanine ) (GAH) (p51- nedasin)

Miscellaneous proteins AAA59434a Human Ig rearranged H-chain mRNA V-region, 11312 5 69 1 Partial cds (Fragment) – Homo sapiens O15540 FABB_HUMAN Fatty acid-binding protein, brain (B-FABP) (Brain 14994 10 104 1 lipid-binding protein) (BLBP) (Mammary derived growth inhibitor related) P35527 K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) 62178 9 67 1 (CK 9) Q14194 DPY1_HUMAN Dihydropyrimidinase related protein-1 (DRP-1) 62487 16 130 2 (Collapsin response mediator protein 1) (CRMP-1). Q14195 DPY3_HUMAN Dihydropyrimidinase related protein-3 (DRP-3) (Unc- 62323 18 95 1 33-like phosphoprotein) (ULIP protein) (Collapsin response mediator protein 4) (CRMP-4) Q16555 DPY2_HUMAN Dihydropyrimidinase related protein-2 (DRP-2) 62711 30 274 13 (Collapsin response mediator protein 2) (CRMP-2) (N2A3) Q8WUM4 PD6I_HUMAN Programmed cell death 6 interacting protein (ALG-2 96646 9 79 1 interacting protein 1) (Hp95) Q96TG8 Q96TG8 HUNC18b 69091 25 196 2 Q99719 SEP5_HUMAN Septin 5 (Peanut-like protein 1) (Cell division control 43206 14 95 1 related protein 1) (CDCREL-1)

Proteolytic proteins 1LYWBa Cathepsin D (EC 3.4.23.5), chain B 26457 21 205 1 O00487 O00487 26S proteasome-associated pad1 homolog 34726 14 85 1 P09936 UBL1_HUMAN Ubiquitin carboxyl-terminal hydrolase isozyme L1 25151 12 127 2 (EC 3.4.19.12) (UCH-L1) (Ubiquitin thiolesterase L1) (Neuron cytoplasmic protein 9.5) (PGP 9.5) (PGP9.5) P25786 PSA1_HUMAN Proteasome subunit alpha type 1 (EC 3.4.25.1) 29864 10 63 1 (Proteasome component C2) (Macropain subunit C2) (Multicatalytic endopeptidase complex subunit C2) (Proteasome nu chain) (30 kDa prosomal protein) (PROS-30) P36776 LONM_HUMAN Lon protease homolog, mitochondrial [Precursor] (EC 95533 12 79 1 3.4.21.-) (Lon protease-like protein) (LONP) (LONHs) P51665 PSD7_HUMAN 26S proteasome non-ATPase regulatory subunit 7 37060 8 66 1 (26S proteasome regulatory subunit S12) (Proteasome subunit p40) (Mov34 protein homolog) Q16781 UBCN_HUMAN Ubiquitin-conjugating enzyme E2 N (EC 6.3.2.19) 17184 7 93 1 (Ubiquitin-protein ligase N) (Ubiquitin carrier protein N) (Ubc13) (Bendless-like ubiquitin conjugating enzyme) Q96KP4 CGL1_HUMAN Cytosolic nonspecific dipeptidase (Glutamate 53161 15 91 1 carboxypeptidase-like protein 1)

Signaling proteins P04901 GBB1_HUMAN Guanine nucleotide-binding protein G(I)/G(S)/G(T) 38151 14 86 1 Jae-Won Yang 21

Identification Number of Number Entry name Protein name (Synonym) Mr identified Matches Scoreb spot beta subunit 1 (Transducin beta chain 1) (37,4) P21796 POR1_HUMAN Voltage-dependent anion-selective channel protein 1 30731 12 120 2 (VDAC-1) (hVDAC1) (Outer mitochondrial membrane protein porin 1) (Plasmalemmal porin) (Porin 31HL) (Porin 31HM) P28482 MK01_HUMAN Mitogen-activated protein kinase 1 (EC 2.7.1.37) 40794 15 111 1 (Extracellular signal-regulated kinase 2) (ERK-2) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2) (p42-MAPK) (ERT1) P30086 PEBP_HUMAN Phosphatidylethanolamine-binding protein (PEBP) 21027 8 91 2 (Prostatic binding protein) (HCNPpp) (Neuropolypeptide h3) (Raf kinase inhibitor protein) (RKIP) (Hippocampal cholinergic neurostimulating peptide) (HCNP) P45880 POR2_HUMAN Voltage-dependent anion-selective channel protein 2 32060 12 127 2 (VDAC-2) (hVDAC2) (Outer mitochondrial membrane protein porin 2) P50395 GDIB_HUMAN Rab GDP dissociation inhibitor beta (Rab GDI beta) 51087 32 288 2 (GDI-2) Q01518 CAP1_HUMAN Adenylyl cyclase-associated protein 1 (CAP 1) 51926 8 78 1 Q02750* MPK1_HUMAN Dual specificity mitogen-activated protein kinase 43622 18 114 1 kinase 1 (EC 2.7.1.-) (MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1) Q9Y3F5 Q9Y3F5 A6 related protein (Protein tyrosine kinase 9-like) 39751 13 124 1 (A6-related protein)

Synaptosomal and neuronal proteins Q05193 DYN1_HUMAN Dynamin-1 (EC 3.6.1.50) 97543 27 194 4 P00915 CAH1_HUMAN Carbonic anhydrase I (EC 4.2.1.1) (Carbonate 28408 14 164 1 dehydratase I) (CA-I) (Carbonic anhydrase B) P00918 CAH2_HUMAN Carbonic anhydrase II (EC 4.2.1.1) (Carbonate 28665 11 116 1 dehydratase II) (CA-II) (Carbonic anhydrase C) P07197 NFM_HUMAN Neurofilament triplet M protein (160 kDa 102312 13 107 1 neurofilament protein) (Neurofilament medium polypeptide) (NF-M) (Neurofilament 3) P14136 GFAP_HUMAN Glial fibrillary acidic protein, astrocyte (GFAP) 49907 10 95 6 P21579 SYT1_HUMAN Synaptotagmin I (SytI) (p65) 47885 17 114 1 P37840 SYUA_HUMAN Alpha-synuclein (Non-A beta component of AD 11368 4 71 1 amyloid) (Non-A4 component of amyloid precursor) (NACP) P46459 NSF_HUMAN Vesicle-fusing ATPase (EC 3.6.4.6) (Vesicular-fusion 83113 14 125 4 protein NSF) (N-ethylmaleimide sensitive fusion protein) (NEM-sensitive fusion protein) Q15121 PE15_HUMAN Astrocytic phosphoprotein PEA-15 (Phosphoprotein 15088 7 81 1 enriched in ) (PED) Q16181 SEP7_HUMAN Septin 7 (CDC10 protein homolog) 49041 14 141 1 Q92777 SYN2_HUMAN Synapsin II 52649 13 94 1 Q9GZT8 NF3L_HUMAN NIF3-like protein 1 (Amyotrophic lateral sclerosis 2 39244 15 104 1 chromosomal region candidate gene protein 1) (My018 protein) (MDS015) Q9UH03 SEP3_HUMAN Neuronal-specific septin 3 38433 8 61 1

Transcriptional proteins O14805 O14805 RNA-binding protein regulatory subunit 20050 9 104 2 P10159 IF5A_HUMAN Eukaryotic translation initiation factor 5A (eIF-5A) 16918 6 72 1 (eIF-4D) (Rev-binding factor) P14651 HXB3_HUMAN Homeobox protein Hox-B3 (Hox-2G) (Hox-2.7) 8111 4 62 1 P22626 ROA2_HUMAN Heterogeneous nuclear ribonucleoproteins A2/B1 36041 11 102 1 (hnRNP A2 / hnRNP B1) P46109 CRKL_HUMAN Crk-like protein 33870 7 66 1 P49411 EFTU_HUMAN Elongation factor Tu, mitochondrial [Precursor] (EF- 49852 22 185 2 Tu) (P43) Jae-Won Yang 22

Identification Number of Number Entry name Protein name (Synonym) Mr identified Matches Scoreb spot P55795* ROH2_HUMAN Heterogeneous nuclear ribonucleoprotein H' (hnRNP 49517 16 145 1 H') (FTP-3) Q9UNT0 Q9UNT0 Silencing information regulator 2-like protein (SIR2 40117 18 182 1 (Silent mating type information regulation 2, S.CEREVISIAE, homolog)-like) a Accession number of NCBI database b Score is –10*Log (P), where P is the probability that the observed match is a random event. Protein scores greater than 61 are significant (p<0.05) (MASCOT, http://www.matrixscience.com). *Identified result from combination of MS and MS/MS database search (12 proteins).