Parallel Evolution in the Major Haemoglobin Genes of Eight Species of Andean Waterfowl
Molecular Ecology (2009) doi: 10.1111/j.1365-294X.2009.04352.x Parallel evolution in the major haemoglobin genes of eight species of Andean waterfowl K. G. M C CRACKEN,* C. P. BARGER,* M. BULGARELLA,* K. P. JOHNSON,† S. A. SONSTHAGEN,* J. TRUCCO,‡ T. H. VALQUI,§– R. E. WILSON,* K. WINKER* and M. D. SORENSON** *Institute of Arctic Biology, Department of Biology and Wildlife, and University of Alaska Museum, University of Alaska Fairbanks, Fairbanks, AK 99775, USA, †Illinois Natural History Survey, Champaign, IL 61820, USA, ‡Patagonia Outfitters, Perez 662, San Martin de los Andes, Neuque´n 8370, Argentina, §Centro de Ornitologı´a y Biodiversidad (CORBIDI), Sta. Rita 117, Urbana Huertos de San Antonio, Surco, Lima 33, Peru´, –Museum of Natural Science, Louisiana State University, Baton Rouge, LA 70803, USA, **Department of Biology, Boston University, Boston, MA 02215, USA Abstract Theory predicts that parallel evolution should be common when the number of beneficial mutations is limited by selective constraints on protein structure. However, confirmation is scarce in natural populations. Here we studied the major haemoglobin genes of eight Andean duck lineages and compared them to 115 other waterfowl species, including the bar-headed goose (Anser indicus) and Abyssinian blue-winged goose (Cyanochen cyanopterus), two additional species living at high altitude. One to five amino acid replacements were significantly overrepresented or derived in each highland population, and parallel substitutions were more common than in simulated sequences evolved under a neutral model. Two substitutions evolved in parallel in the aA subunit of two (Ala-a8) and five (Thr-a77) taxa, and five identical bA subunit substitutions were observed in two (Ser-b4, Glu-b94, Met-b133) or three (Ser-b13, Ser-b116) taxa.
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