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11-8011: Polyclonal Antibody to -8

Clonality : Polyclonal Application : WB Reactivity : Human : IPO8 Gene ID : 10526 Uniprot ID : O15397 Format : Purified Alternative Name : IPO8,RANBP8 Isotype : Rabbit IgG A partial length recombinant Importin-8 (amino acids 700-1,000) was used as the Immunogen Information : immunogen for this antibody.

Description

Importin-8 is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran- binding site of importin-beta. It acts as a nuclear protein involved in the nucleolar transport. Importin-8 is required for cytoplasmic miRNA-guided gene silencing and is involved in loading Ago complexes into a variety of different mRNA targets, affecting the nuclear localization of Ago . This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. Importin-8 has been proposed previously as a reference gene in the human motor cortex as it showed no differences in a study with different brain regions from chronic alcoholics and control subjects and lung samples.

Product Info

Amount : 25 µg / 100 µg Purification : Protein A Chromatography 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium Content : azide is highly toxic. Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid Storage condition : repeated freeze and thaw cycles.

Application Note

Western blot analysis: 2-4 µg/ml

Fig-1: Western blot analysis of Importin-8. Anti- Importin-8 antibody (11-8011) was used at 2 µg/ml on Jurkat lysate.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.