THE COMMONWEALTH OF MASSACHUSETTS STATE RECLAMATION & MOSQUITO CONTROL BOARD

CENTRAL MASSACHUSETTS MOSQUITO CONTROL PROJECT

111 Otis Street, Northborough, MA 01532-2414 Telephone (508) 393-3055  Fax (508) 393-8492 www.cmmcp.org

ANNUAL REPORT 2016

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PREFACE

The 2016 Annual Report of the Central Massachusetts Mosquito Control Project (the Project) has been prepared to provide the citizens and officials of the member cities and towns with information pertaining to the Project's control procedures and related activities.

As you read through this report you will notice that the Project is committed to an Integrated Pest Management (IPM) program. IPM utilizes a variety of control techniques and evaluation procedures. All control efforts are undertaken only after surveillance data has been collected and analyzed. This allows control decisions to be made based on the exact need that exists at each specific site. Environmental considerations are paramount when prescribing various control techniques.

The CMMCP Board of Commission is appointed by the State Reclamation and Mosquito Control Board to represent your community's interest. The Commissioners meet with the Executive Director and Director of Operations on a regular basis to discuss and formulate policies, and to provide their expertise in the operation of the Project. The Commissioners welcome your input, and we encourage you to schedule an appointment to visit our Project headquarters.

Copies of this report are available to key officials and departments in our member communities, as well as to the public libraries. We would encourage officials to take time from their busy schedule to read this report. Project personnel are available to answer questions you may have, and to meet with you to discuss out procedures and techniques. The Project’s website at www.cmmcp.org has extensive information on mosquito control in Central Massachusetts.

The Project's goal is to provide effective and environmentally sound mosquito control, reducing mosquito annoyance and the potential for the transmission of mosquito-borne diseases. Our staff of competent, well-trained employees are known throughout the member communities as individuals who take great pride in their work.

Thank you,

Richard J. Day, Chair Board of Commissioners Central Massachusetts Mosquito Control Project

Partner, Member, Member, EPA Pesticide Partner, Northeastern New Jersey Environmental Mosquito Control EPA WasteWise Mosquito Control Stewardship Program Program Association Association

Member, Massachusetts Municipal Association Member, MassRecycle

THE COMMONWEALTH OF MASSACHUSETTS

State Reclamation & Mosquito Control Board 251 Causeway Street Suite 500 Boston, Massachusetts 02114

http://www.mass.gov/agr/mosquito/

State Reclamation Board - Members

Ms. Alisha Bouchard (DAR) - Chair

Mr. James Straub (DCR)

Mr. Gary Gonyea (DEP)

Commissioners of Central Massachusetts Mosquito Control Project [email protected]

Mr. Richard J. Day - Chair Chelmsford, Massachusetts

Hon. Dean Mazzarella Leominster, Massachusetts

Mr. Pablo E. Noguera Billerica, Massachusetts

Mr. Paul Mazzuchelli Milford, Massachusetts

Dr. Sam Telford Shrewsbury, Massachusetts

Administrative Staff of Central Mass. Mosquito Control Project www.cmmcp.org

Executive Director Mr. Timothy D. Deschamps [email protected]

Director of Operations Mr. Timothy E. McGlinchy [email protected]

Office Manager Ms. Ellen Holmes [email protected]

Staff Entomologist Mr. Curtis R. Best [email protected]

Staff Biologist Mr. Frank Cornine III [email protected]

Field Biologist Mr. Todd Duval (January - May) Mr. David Mullins (December - ) [email protected]

Wetlands Project Coordinator Ms. Katrina Proctor [email protected]

LIST OF MEMBER COMMUNITIES - 2016

TOWN SQUARE MILES

DISTRICT ONE

BILLERICA 25.96 CHELMSFORD 22.70 DRACUT 20.90 LOWELL 14.50 LITTLETON 16.60 TEWKSBURY 20.70 WESTFORD 30.60 WILMINGTON 17.12

DISTRICT TWO

ACTON 20.00 AYER 9.00 BOXBOROUGH 10.40 DEVENS 5.28 FITCHBURG 27.80 GARDNER 23.00 LANCASTER 27.70 LEOMINSTER 28.90 LUNENBURG 26.40 STOW 17.60

DISTRICT THREE

BERLIN 12.90 BOYLSTON 16.00 CLINTON 5.70 HUDSON 11.50 MARLBOROUGH 21.10 NORTHBOROUGH 18.50 SHREWSBURY 20.70 SOUTHBOROUGH 14.10

DISTRICT FOUR

ASHLAND 12.40 HOLLISTON 18.70 HOPEDALE 5.27 HOPKINTON 26.60 MILFORD 14.60 NATICK 15.10 SHERBORN 16.00 WESTBOROUGH 20.50

DISTRICT FIVE

AUBURN 15.40 BLACKSTONE 10.90 MILLBURY 15.70 MILLVILLE 4.92 NORTHBRIDGE 17.20 STURBRIDGE 37.40 WEBSTER 12.50

Total Square Miles 728.85

NEW HAMPSHIRE

Winchendon Ashby Townsend Pepperell Dunstable DRACUT Ashburnham Tyngsboro LOWELL

FITCHBURG Groton TEWKSBURY LUNENBURG WESTFORD CHELMSFORD GARDNER Templeton Shirley AYER BILLERICA WILMINGTON Westminster DEVENS LITTLETON LEOMINSTER Harvard Hubbardston LANCASTER BOXBORO ACTON

Princeton Sterling Bolton STOW Barre CLINTON

Rutland W. BERLIN Boylston HUDSON BOYLSTON Oakham Holden MARLBORO

NORTHBORO Paxton SOUTHBORO SHREWSBURY NATICK Spencer Worcester WESTBORO ASHLAND Leicester SHERBORN HOPKINTON AUBURN Grafton HOLLISTON MILLBURY

Upton MILFORD Charlton Oxford Sutton NORTHBRIDGE STURBRIDGE HOPEDALE

Mendon Uxbridge

Southbridge Dudley WEBSTER Douglas MILLVILLE

BLACKSTONE

CONNECTICUT RHODE ISLAND

N

= member towns W E

S MOSQUITO CONTROL ACTIVITIES

One basic fact of the mosquito’s biology is the dependence on still, stagnant water to complete its life cycle from egg to adult. One method employed is called ‘‘water management or ‘‘ditch maintenance’’. This method reduces or eliminates the source of potential mosquito larval habitat, and consists of cleaning road-side ditches and culverts, removal of brush and accumulated debris from ditches. This method permits water to flow freely and reduces the likelihood for stagnant areas, areas in which the larval mosquito needs to develop. This program is practiced year-round, and is done only after extensive examination by our wetland scientist and permission is received by the property owner(s).

There are places where water management is neither practical nor feasible for one reason or another. In these situations, we practice a control method for mosquito larvae called ‘‘larviciding’’. After a field technician has determined that larval mosquitoes are present, a small amount of environmentally sensitive product (usually a bacteria) is applied to the area according to label directions. This is often a very effective control method, reducing the emergence of the adult mosquito from that area. Larviciding is practiced from March to September or October as conditions warrant.

A third method is to attempt to control the adult mosquito. The control of adult mosquitoes is called ‘adulticiding’’ and is done on a request-only basis, and the presence of adult mosquitoes is confirmed before any application is done. Adulticiding can be an effective method of temporary control, which can be beneficial prior to public gatherings, outdoor events and festivals, or when mosquito populations have been determined to be intolerable. Since this part of the program is done only upon request, this allows the individual resident to have the ultimate discretion on mosquito spraying in their area - how much or how little. Exemptions for spraying are handled through the City/Town Clerk and the Project office, and are updated each year. Adulticiding is done from approximately Memorial Day to Labor Day, depending on prevalent mosquito populations and the mosquito- borne disease situation. All products used by the Project have been extensively tested by manufacturers, the US government and mosquito control agencies for many years. They are registered by the EPA and the Mass. Pesticide Bureau. Labels and fact sheets are available upon request to the public from the Project’s office, our technicians or from our website.

We operate a full surveillance program in our service area. The landing rates performed by our field staff are brought back to the Project lab to be keyed out to , allowing us to tailor our larviciding program and reduce future dependence on adulticides. We have a mobile team of specialized mosquito traps, called ‘‘gravid traps’’, designed to capture virus-bearing mosquitoes. These mosquito collections, called ‘‘pools’’, are sent into the Mass. Dept. of Public Health (MDPH) laboratory in Jamaica Plain for testing of West Nile Virus, Eastern Equine Encephalitis, and other arboviruses of concern by MDPH. These traps are used in a rotation throughout our service area, and are then concentrated in areas showing arboviral activity to supplement MDPH’s collection protocols. Additional trap types are utilized in suspect areas to monitor and evaluate the risk of viral transmission from mosquitoes to the local populace.

A comprehensive educational program is offered to area schools and civic groups. The program is aimed towards mosquito biology, mosquito habitat, and efforts citizens can undertake to reduce the potential for mosquito populations in their own neighborhood. This program is tailored to suit the requirements of the individual group, from elementary school children, to high school, to adult groups. A new program for senior citizens was established in 2011.

‘‘Source reduction’’ is reducing or eliminating the source of mosquito larval habitat. We offer a tire recycling program in our member communities at no additional cost to residents because used tires in the environment are larval habitat for several mosquito species, some of which carry West Nile Virus.

PROGRAM EVALUATION

This is a part of the program which many people involved directly never see. It must begin with a carefully planned program, one designed so that the data obtained during surveys before treatment and the surveys taken after treatment can be analyzed by statistically sound methods. Only by doing this can the value of a mosquito control program be determined. We will then know what type (species) of mosquito we are dealing with; what the population density is; what method(s) of control provide the most economical and efficient results. Then and only then can we say that we have or have not affected mosquito control on a level that is acceptable to the community.

SEASONAL OUTLINE OF MOSQUITO CONTROL PROGRAM

1. Wetlands Restoration/Ditch maintenance - throughout the year, intensified September through February

2. Public Education - throughout the year, intensified April through August

3. Program Preparation - December through March

4. Equipment Maintenance - December through February

5. Research & Efficacy - April through October

5. Larval Control (wetlands) - March through September (aerial work is only done in 3 towns at this time and by supplemental funding)

6. Larval Control (catch basins) - June through September

7. Adult mosquito Surveillance - May through September/October

8. Adulticiding - June through September/October

9. Source reduction - throughout the year, intensified September through February

Any mosquito control being done by individual member communities must, by law, be coordinated through the Central Massachusetts Mosquito Control Project. SERVICES AND ACTIVITIES

The following services and activities are available to those communities participating in the Central Massachusetts Mosquito Control Project:

1. LARVAL CONTROL: Wetlands and suspected mosquito breeding sites are monitored from March through September to determine the need for applications of environmentally sensitive products (typically a bacteria called Bti) to control and/or eliminate the larval mosquito. By controlling mosquitoes in their larval stage the need for adult mosquito spraying is reduced.

2. SOURCE REDUCTION: Reducing or removing larval habitat by recycling, waste disposal or other means is a permanent solution. Mosquito larvae are opportunistic and will create habitat in any container that holds water for more than a week. Empty and clean birdbaths and kiddie pools each week, cover or store inside anything that may capture and hold water, and dispose of or recycle any containers that are no longer needed. CMMCP now has a tire recycling program to allow residents a means to dispose of these important larval habitats.

3. WETLAND RESTORATION/DITCH MAINTENANCE: Mosquitoes need still, stagnant water to complete their metamorphosis from egg to adult. CMMCP conducts maintenance on ditches, culverts and man-made ponds to improve water quality and increase water flow, reducing the potential for mosquito breeding.

4. SURVEILLANCE: Mosquito populations are monitored in both the larval and adult stages to determine the appropriate control methods to be employed, prevalent mosquito species, and disease transmission potential. CMMCP has instituted a program to supplement the Dept. of Public Health's arbovirus surveillance program for monitoring West Nile Virus in Massachusetts, using mosquito gravid traps. These traps will be placed throughout out service area and can be quickly broken down and moved to respond to the immediate needs of monitoring for this and other mosquito-borne diseases. When WNV or EEE is confirmed in a member city or town, these traps are placed in areas that have been determined to harbor this virus. Additional types of traps able to sample mammal-biting mosquitoes will also be placed to determine WNV levels and risk to the local populace.

5. PUBLIC EDUCATION: Educating the public about mosquitoes and their biology is an important aspect of our program. We offer a comprehensive program in member communities geared towards school-aged children from Kindergarten to High School. This program is tailored to meet the needs of intended audience. In 2011 we developed a specialized program geared towards senior citizens. The Project produces public relations handouts, and all member Town Halls are stocked with information on CMMCP, our programs, and how the homeowner can reduce mosquito populations in their own area. Project staff is available to meet with civic organizations, town/city boards, and to participate in Health Fairs. Tours of the Project's headquarters can be arranged by calling our office.

6. ADULT MOSQUITO CONTROL: When adult mosquito populations reach intolerable levels, hand- held or truck mounted sprayers are used to reduce the adult mosquito levels in residential areas. CMMCP has worked diligently over the past 20 years to achieve the goal of reducing the dependency on adulticiding by increasing the emphasis on larviciding, public education, water management and source reduction.

7. BEAVER MITIGATION: CMMCP receives many requests from city and town officials and property owners seeking assistance to alleviate flooding cause by beaver activity. CMMCP recognizes beavers as keystone species of the natural and ecological landscape. Beaver activity creates wetland and wildlife habitat that benefits many plant and species. Along with the positive aspects of beaver activity come some negative aspects. The most frequent issue affecting CMMCP is dam building activity which clogs culverts and drainage ditches. Increased flooding creates new habitat for mosquitoes and increases the need for Integrated Pest Management (IPM) techniques. CMMCP supports and follows the recommended practices for beaver management per the Massachusetts Division of Fisheries and Wildlife. Beaver activity will not be interrupted unless it becomes a threat to public health and safety per the Massachusetts Beaver Law M.G.L. c. 131 S. 80A. CMMCP will fully adhere to the permitting process as regulated by the Division of Fisheries and Wildlife (DFW) and the Department of Public Health (DPH).

8. RESEARCH AND EFFICACY: While CMMCP is an agency charged with the control of mosquitoes, we strive to check for efficacy of our products and techniques, and whenever possible perform research in new or different areas of mosquito control. CMMCP MEDICAL ENTOMOLOGY LABORATORY REPORT, 2016

The mission of the Medical Entomology Laboratory is to refine and maximize the Central Massachusetts Mosquito Control Project’s ongoing effort to control mosquitoes. During 2016 Medical Entomology Laboratory personnel carried this mission forward in the following ways.

The Staff Entomologist made 78 educational presentations before 3,150 elementary school students in 12 Elementary schools. The students learned about the life cycle and biology of mosquitoes. They also learned what they could do to control the mosquito population around their own home and how to protect themselves from nuisance mosquitoes. During 2016, three technicians were employed for the season to operate the mosquito surveillance traps. Using their knowledge of mosquito behavior and the local terrain, these skilled and experienced personnel monitored the adult mosquito population. Approximately 1,477 collections were made during 2016.

Collections of mosquitoes were made using Modified Reiter Gravid Traps, BG Sentinel Traps, OVI Cups and New Standard Miniature Light Traps. Modified Reiter Gravid Traps are attractive to Culex mosquito species. Culex species are implicated in the maintenance and transmission of West Nile virus in Massachusetts. BG Sentinel traps are attractive to a mosquito species named Aedes albopictus. Aedes albopictus, commonly known as the “Asian Tiger Mosquito”, is an invasive mosquito species that is threatening to make in roads into Massachusetts. The species is an aggressive daytime biter and has proven capable of carrying and transmitting a variety of viral diseases. No adult Aedes albopictus were collected during 2016. OVI Cups are plastic cups with a paper liner that are set out for a week at a time after being filled with water. These cups are attractive to female Aedes albopictus seeking a site to lay their eggs. The papers are later examined for the presence of eggs. Any eggs collected are hatched out and the immature mosquitoes reared until they can be identified. No Aedes albopictus eggs were collected during 2016. New Standard Miniature Light Traps use light and/or carbon dioxide gas to attract the vectors of both West Nile and Eastern Equine Encephalitis. The addition of carbon dioxide gas results in larger collections. Eastern Equine Encephalitis is caused by a virus that has been found in a variety of mosquito species. Ongoing research implicates Culiseta melanura, as the most important vector of Eastern Equine virus. Culiseta melanura utilizes Red Maple swamps as a breeding habitat. Red Maple swamps are found throughout the CMMCP service area.

The collected mosquitoes were identified to species by the Staff Entomologist and Summer Intern. Mosquito species known to play a role in the transmission of disease were set aside for further processing. During 2016, 52,623 mosquitoes representing 19 species were submitted for testing. For testing purposes they were divided into 1,345 groups or pools. These pools of mosquitoes were tested for West Nile virus and Eastern Equine virus infection. Of the 1,345 pools tested sixteen proved positive for West Nile virus. None of the pools proved positive for Eastern Equine virus. The findings are listed below.

In response to the positive test results the CMMCP increased surveillance of mosquitoes in these areas. Mosquito control measures were augmented as well. The data from these collections was shared with the Massachusetts Department of Public Health.

Modern, scientifically based mosquito control has many facets. These include public education, surveillance, water management and control of immature and adult mosquitoes. Medical Entomology Laboratory personnel are committed to advancing all facets of mosquito control. Such a commitment will further enable the Central Massachusetts Mosquito Control Project to provide its member communities with quality mosquito control.

Respectfully, Curtis R. Best Staff Entomologist

2016 Arbovirus Surveillance Results – CMMCP

Collection Date Species Town Result 8/2/2016 Culex species Auburn Positive WNV 8/2/2016 Coquillettidia perturbans Auburn Positive WNV 8/5/2016 Coquillettidia perturbans Hopkinton Positive WNV 8/16/2016 Culex species Chelmsford Positive WNV 8/19/2016 Culex species Millbury Positive WNV 8/23/2016 Culex species Auburn Positive WNV 8/24/2016 Culex species Westborough Positive WNV 8/25/2016 Culex species Boylston Positive WNV 8/25/2016 Culex species Millbury Positive WNV 8/25/2016 Culex species Millbury Positive WNV 9/1/2016 Culex species Millbury Positive WNV 9/7/2016 Culex species Auburn Positive WNV 9/8/2016 Culex species Millbury Positive WNV 9/8/2016 Culex species Berlin Positive WNV 9/13/2016 Culex species Auburn Positive WNV 9/15/2016 Culex species Clinton Positive WNV

CMMCP Surveillance Summary 2016 Mosquitoes Collected 52,623 Mosquito Pools Submitted for Testing 1,345 Mosquito Pools Positive WNv 16 Mosquito Pools Positive EEv 0 WNv Surveillance Summary - Statewide 2016 Mosquito Pools Positive WNv 189 Positive WNv 0 Humans Positive WNv 15 EEv Surveillance Summary - Statewide 2016 Mosquito Pools Positive EEv 4 Animals Positive EEv 0 Humans Positive EEv 0

Enhanced Surveillance for Aedes albopictus - Ovitrap Collections

# Ovitraps # Egg Papers # Eggs EPI Week #22 15 7 0 EPI Week #23 - - - EPI Week #24 5 2 49 EPI Week #25 15 6 93 EPI Week #26 17 17 19 EPI Week #27 25 19 1180 EPI Week #28 25 25 1020 EPI Week #29 10 7 62 EPI Week #30 15 12 632 EPI Week #31 15 10 524 EPI Week #32 20 19 985 EPI Week #33 10 10 147 EPI Week #34 35 33 1929 EPI Week #35 30 20 263 EPI Week #36 20 20 27 EPI Week #37 30 28 896 EPI Week #38 20 20 181 EPI Week #39 30 30 14 2016 Totals 337 285 8021

No Ae. albopictus specimens were identified in 2016.

Auburn Surveillance Data 2016

Town Pool ID Collection Date Trap Site Pool Size Species Result Auburn CM16-0007 6/7/2016 Anderson Way 33 Cx. pipiens/restuans Negative Auburn CM16-0008 6/7/2016 Rochdale St. 10 Oc. canadensis Negative Auburn CM16-0168 6/21/2016 Rochdale St. 37 Cx. pipiens/restuans Negative Auburn CM16-0169 6/21/2016 Rochdale St. 6 Oc. japonicus Negative Auburn CM16-0170 6/21/2016 Anderson Way 40 Cq. perturbans Negative Auburn CM16-0251 6/28/2016 Maywood Cir. 14 Cx. pipiens/restuans Negative Auburn CM16-0252 6/28/2016 Berlin St. 50 Cq. perturbans Negative Auburn CM16-0344 7/6/2016 Athens St. 50 Cq. perturbans Negative Auburn CM16-0345 7/6/2016 Athens St. 50 Oc. canadensis Negative Auburn CM16-0419 7/12/2016 Tuck Farm Rd. 7 Cx. pipiens/restuans Negative Auburn CM16-0420 7/12/2016 Tuck Farm Rd. 5 Oc. japonicus Negative Auburn CM16-0422 7/12/2016 Santom St. 17 Cq. perturbans Negative Auburn CM16-0429 7/13/2016 Auburn Hill Rd. 16 Cx. pipiens/restuans Negative Auburn CM16-0432 7/13/2016 Washington St. 5 Cx. pipiens/restuans Negative Auburn CM16-0433 7/13/2016 Springbrook Rd. 50 Cx. pipiens/restuans Negative Auburn CM16-0434 7/13/2016 Springbrook Rd. 16 Cx. pipiens/restuans Negative Auburn CM16-0436 7/13/2016 Rydberg Ter. 41 Cx. pipiens/restuans Negative Auburn CM16-0514 7/19/2016 Heard St. 50 Cx. pipiens/restuans Negative Auburn CM16-0515 7/19/2016 Heard St. 13 Cx. pipiens/restuans Negative Auburn CM16-0517 7/19/2016 Springbrook Rd. 50 Cq. perturbans Negative Auburn CM16-0518 7/19/2016 Springbrook Rd. 50 Cq. perturbans Negative Auburn CM16-0619 7/26/2016 Heard St. 9 Cx. pipiens/restuans Negative Auburn CM16-0623 7/26/2016 Springbrook Rd. 50 Cq. perturbans Negative Auburn CM16-0723 8/2/2016 Rydberg Ter. 43 Cx. pipiens/restuans Negative Auburn CM16-0724 8/2/2016 Auburn Hill Rd. 24 Cx. pipiens/restuans Positive WNV Auburn CM16-0725 8/2/2016 Auburn Hill Rd. 13 Cq. perturbans Positive WNV Auburn CM16-0807 8/9/2016 Springbrook Rd. 17 Cx. pipiens/restuans Negative Auburn CM16-0808 8/9/2016 Maywood Cir. 23 Cq. perturbans Negative Auburn CM16-0915 8/16/2016 Auburn Hill Rd. 27 Cx. pipiens/restuans Negative Auburn CM16-1020 8/23/2016 Rydberg Ter. 6 Culex species Negative Auburn CM16-1021 8/23/2016 Rydberg Ter. 50 Culex species Positive WNV Auburn CM16-1022 8/23/2016 Rydberg Ter. 12 Culex species Negative Auburn CM16-1124 8/30/2016 Washington St. 5 Oc. japonicus Negative Auburn CM16-1125 8/30/2016 Washington St. 5 Oc. triseriatus Negative Auburn Surveillance Data 2016

Town Pool ID Collection Date Trap Site Pool Size Species Result Auburn CM16-1201 9/7/2016 Springbrook Rd. 14 Culex species Positive WNV Auburn CM16-1202 9/7/2016 Springbrook Rd. 6 Oc. japonicus Negative Auburn CM16-1260 9/13/2016 Rochdale St. 6 Oc. japonicus Negative Auburn CM16-1261 9/13/2016 Anderson Way 5 Oc. triseriatus Negative Auburn CM16-1262 9/13/2016 Auburn Hill Rd. 50 Culex species Negative Auburn CM16-1263 9/13/2016 Auburn Hill Rd. 20 Culex species Negative Auburn CM16-1264 9/13/2016 Auburn Hill Rd. 11 Culex species Positive WNV Auburn CM16-1265 9/13/2016 Auburn Hill Rd. 36 Ae. vexans Negative Auburn CM16-1301 9/20/2016 Rydberg Ter. 10 Culex species Negative Auburn CM16-1302 9/20/2016 Rydberg Ter. 50 Culex species Negative Auburn CM16-1303 9/20/2016 Rydberg Ter. 5 Culex species Negative Auburn CM16-1345 9/27/2016 Auburn Hill Rd. 10 Culex species Negative Auburn CM16-1346 9/27/2016 Auburn Hill Rd. 9 Ae. vexans Negative

47 collections 884 mosquitoes collected 47 collections submitted for testing 884 submitted for testing Town of Auburn: Mosquito Surveillance Program 2016 Ü

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CMMCP (2016) Select features of this map courtesy of: ^_ Surveillance Trap Sites SUTTON Office of Geographic Information (MassGIS), Commonwealth of Massachusetts Information Technology Division Potential Mosquito Breeding Wetlands WETLANDS RESTORATION PROGRAM REPORT 2016

INTENT AND PURPOSE OF PROGRAM:

Wetland restoration is an important part of the CMMCP’s Integrated Pest Management (IPM) plan for mosquito control. The intent of the program is to improve the flow of water in degraded drainage systems through ditch maintenance and wetland restoration projects. These projects will effectively reduce and prevent mosquito breeding sources and can reduce or often eliminate the need for periodic applications of pesticides.

Wetland restoration/water management projects are planned per the Massachusetts Best Management Practices and Guidance for Freshwater Mosquito Control and the Mechanical Wetlands Management Activity Post-Monitoring Guidelines. Wetlands projects are designed to minimize wetlands impacts.

Projects are initiated with a phone call from a town resident or town official. Also, a member from the CMMCP staff may identify a site that could benefit from work. Once a site is brought to the attention of CMMCP, the Wetland Project Coordinator performs an assessment of the site. If the site is appropriate for work, a site survey, site plan, and notifications are completed.

The site survey includes soil sampling, taking transects and cross sections of the ditch, and determining hydrological conditions. Wetlands are classified and sites are documented in the pre- and post- excavation states through a photographic record. Historical information on the drainage system is obtained from local residents or town records. The data gathered in the field is used in combination with information acquired from resources such as historical aerials and spatial data from the Commonwealth of Massachusetts Office of Geographic Information (MassGIS) online mapping program to develop a project site plan. The site plan includes project specifications which the field staff need in order to properly perform the project.

Once the site plan is completed, notification letters and permission slips are sent out to all property owners who would be affected by the project. In addition, notification letters are sent to MA DEP, the local conservation commission and the US Army Corps of Engineers for all mechanized work using a low ground pressure excavator. The notification letter provides a 30 day grace period. During this time, property owners and agencies have the opportunity to notify CMMCP of any concerns that they may have with a project. If there are legitimate concerns, a project may be modified, delayed or abandoned. If no issues are brought to the attention of CMMCP within the 30 day period, the project begins as planned.

The presence of beaver in the watershed has become an increasing concern for residents, town officials and CMMCP. Active beaver create beaver dams along streams and within wetland areas creating beaver ponds often resulting in flooding. Increase in flooding may cause health and safety issues for residents and municipalities. Increased flooding typically causes stagnant standing water which is prime mosquito habitat. CMMCP offers assistance and guidance relating to beaver management. CMMCP consults with local boards of health and conservation commissions to comply with state laws. CMMCP is in the practice of removing beaver dams, installing culvert protection and water flow devices on a case by case basis. Trapping is not a provided service at this time.

SUMMARY OF WORK FOR 2016:

In 2016, 98 sites were assessed by the Wetland Project Coordinator. Of these sites, 18 were visited multiple times to best survey, implement, and monitor water management work. Of the sites, 42 were brought to the attention of the Project through resident requests (43%), 24 sites were requested by town officials (25%), and 21 were identified by CMMCP staff (21%). Eleven were requested from a combination of residents, officials, and/or CMMCP staff (11%). In addition, eight new sites were assessed with regard to new beaver complaints (8%).

Forty-two water management jobs were set up and completed, with ongoing maintenance. Fifteen of these jobs involved the use of the low ground pressure excavator. All jobs included some degree of hand work. Additionally, eight sites were accessed for potential beaver management projects. Two small beaver dams were breached by hand. One large double box culvert was cleared of beaver debris. One pond leveler flow device was installed. Four water flow devices were monitored and maintained.

Additional information on our procedures or on specific restoration projects can be acquired by calling the CMMCP office at (508) 393-3055 from 7:00am to 3:30pm.

Respectfully submitted,

Katrina Proctor, Wetland Project Coordinator STAFF BIOLOGIST REPORT 2016

Several research endeavors were undertaken this past season at the Central Massachusetts Mosquito Control Project. The larvicide product Natular™ G30 was evaluated for a second season as a pre-hatch control alternative. This granular larvicide, which utilizes spinosad as its active ingredient, was applied onto the ice or freshly opened water of woodland pools. Treatment sites and control sites were utilized to gauge the efficacy of this product against the larvae of early season mosquito species such as Ochlerotatus abserratus and Ochlerotatus excrucians. Significant control was observed at all the treatment sites, while the control sites maintained a relatively consistent level of mosquito larvae throughout the trial. The results of this project were presented at the 62nd Annual Meeting of the Northeastern Mosquito Control Association (Falmouth, MA).

This season the CMMCP Adulticide Program phased out Anvil® 10+10 and switched to Zenivex® E20 as the primary product used. Unlike Anvil® 10+10 which uses sumithrin as the active ingredient (AI) and piperonyl butoxide (PBO) as a synergist, Zenivex® E20 has etofenprox for the AI and zero PBO synergist. Although both products are synthetic pyrethroids, this change in adulticide prompted a focus shift to the new AI when conducting bottle assays. Without the historical data on local etofenprox resistance, naïve mosquitoes from the CMMCP service area were evaluated to develop a baseline diagnostic concentration. Once this measure was established it could be run with local mosquito populations that have been involved with the CMMCP Adulticide Program. The results of these bottle assays assist in determining if resistance has begun developing and to what degree if any. The limited results from this past season do not indicate a change in product is warranted at this time. Resistance surveillance will continue next season with an expanded range.

Weekly reports for the surveillance program were created throughout the season, highlighting population changes in several target mosquito species, cumulative surveillance numbers, and virus isolations. The egg collection data that was obtained during Aedes albopictus surveillance was also included in the weekly reports for the first time. Upon confirmation of virus isolations, potential adulticide response maps were created for officials to review. Additional maps created this season using the CMMCP GIS included those used for the mosquito surveillance program, aerial larvicide program, water management program, and finally the catch basin larvicide program.

Three presentations on mosquitoes and mosquito-borne disease were conducted at local senior centers this summer. The communities participating were Acton, Clinton, and Southborough. Those in attendance were very enthusiastic and appreciative towards the program. Information on the Zika virus was added to the presentation this year, and will be incorporated into the next version of the booklet that accompanies this presentation. In 2017 presentations will be preferentially offered to CMMCP communities that have not hosted one before and those with 2016 virus isolations before returning to previous locations.

Looking towards the 2017 season, CMMCP will continue evaluating Natular™ G30 as an early season larvicide option, and additionally evaluate Natular™ G as a treatment for Coquillettidia perturbans in retention ponds. Finally, resistance bottle assays for Zenivex® E20 (etofenprox) will be expanded now that a baseline diagnostic dose has been established.

Respectfully submitted,

Frank H. Cornine III Staff Biologist

AN EXAMINATION OF EARLY SEASON TREATMENTS USING NATULAR™ G30

FRANK H. CORNINE III MPH

Central Mass. Mosquito Control Project 111 Otis Street Northborough, MA 01532 (508) 393-3055 • www.cmmcp.org • [email protected]

ABSTRACT

The Central Massachusetts Mosquito Control Project had utilized methoxychlor dust, an organochlorine, as a pre-hatch mosquito larvicide product prior to its discontinued use in the early 1980s. Since that time, CMMCP has not found a suitable replacement product for use against Ochlerotatus abserratus, Oc. excrucians, and to a lesser extent Oc. canadensis. Natular™ G30, a novel larvicide, was chosen to be evaluated as a potential alternative to methoxychlor dust in providing early season control of these mosquito species. Natular™ G30 controls mosquito larvae through the active ingredient spinosad, which is a byproduct of the soil bacteria Saccharopolyspora spinosa. Through field trials conducted in several local communities, CMMCP has determined that certain conditions, Natular™ G30 can be deployed for early season control of target mosquito species.

INTRODUCTION Equine Encephalitis have been detected from this species (Andreadis 2005). Central Massachusetts is scattered with woodland pools, many capable of Historically, CMMCP had utilized the harboring several early season mosquito organochlorine methoxychlor to combat species, including Ochlerotatus spring mosquito species such as Oc. abserratus and Ochlerotatus excrucians. abserratus, Oc. excrucians, and Oc. Both of these “snow pool” species are canadensis. This larvicidal dust was univoltine, meaning they only produce spread onto the ice of know mosquito one generation a year, with any eggs laid habitats during the winter months using during the season remaining dormant rotary spreaders. Adult mosquito until the following spring. Although Oc. emergence would be greatly reduced as abserratus and Oc. excrucians do not a result of these pre-hatch applications. pose a significant threat regarding West At the time of its use, methoxychlor dust Nile virus and Eastern Equine had a wide range of target , over Encephalitis, they are very pestiferous a wide range of sectors including the and greatly contribute to the service agricultural, horticultural and veterinary request calls early in the season by local fields (Extension Toxicology Network residents. Ochlerotatus canadensis is 1996). CMMCP has not used this another species that can develop in this product since the 1980s but also not type of habitat. Unlike Oc. abserratus found an appropriate replacement either. and Oc. excrucians however, Oc. canadensis may produce multiple One relatively novel product that may generations a year. Additionally, help treat early season mosquito species isolations of West Nile virus and Eastern is Natular™ G30. Natular™ G30 is a slow release formulation of spinosad, and is categorized as a Group 5 observations. The application rate for insecticide, as well as a “Reduced Risk” this project was 10lbs/acre with product by the Environmental Protection treatments ranging from 0.1lbs to 10lbs Agency. Spinosad is derived from the of Natular™ G30 depending on the size fermentation of Saccharopolyspora of the particular woodland pool. All final spinosa, a soil bacteria. As indicated by larval observations occurred in mid-April, its name, Natular™ G30 is engineered to past the advertised 30 day control provide effective control for up to 30 window supplied by the product. The days, according to the manufacturer data collected from these field Clarke Mosquito Control Products, Inc. observations was statistically analyzed to (CMMCP 2016). Through field determine whether the Natular™ G30 evaluations of this product, Natular™ significantly controlled mosquito larvae, G30 could become incorporated into the but also whether any changes occurred CMMCP larvicide program as an early at the untreated control sites. In addition season treatment option, expanding the to the larval densities, water window for larviciding and also temperatures and general observations decreasing the amount of adulticide were noted for each RDS during the events in the summer months. course of the Natular™ G30 evaluation.

MATERIALS & METHODS RESULTS

Four communities were included in this Statistical analysis determined that sites field evaluation, each with designated treated with Natular™ G30 experienced treatment and non-treatment sites to a significant (p < 0.05) decrease in demonstrate the efficacy of Natular™ observed mosquito larvae from before G30 against early season mosquito treatment compared to observations larvae. These towns included through the duration of Natular™ G30 Northborough, Southborough, release. When grouped together by Westborough and Hopkinton. A total of town, the treatment sites show steep eighteen treatment sites were used along decrease in larvae following treatment with four untreated control sites. The and remain at near zero throughout, sites used in this project all had a although an increase is recorded after recorded history of early season the active ingredient release period has mosquito larvae activity and the majority past (Figure 1). In contrast the sites not of the sites had been involved in previous applied with Natular™ G30 exhibited no Natular™ G30 field trials. Prior to any significant difference in larval density treatment, four recoverable dip stations prior to treatment with those during the (RDS) were established at all sites. evaluation process. Towards the end of These flagged sites were monitored for the evaluation, larvae at the untreated larval density before treatment and then sites reached late instar and also pupal twice a week throughout the duration of stage. It is possible that some adult the evaluation. The Natular™ G30 emergence may have occurred within the applications at the treatment sites untreated control sites around the occurred on March 8, 2016, immediately conclusion of the project. following initial pretreatment larval

Figure 1: Larval Averages during Natular™ G30 Evaluation

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DISCUSSION use as an early season treatment option would be much greater. Despite the This field evaluation of Natular™ G30 to opportunity for this product to be determine if it would be a suitable early incorporated into the CMMCP larvicide season mosquito larvicide indicates that program, there are many factors that it does have potential for this specific would need to be considered further type of application. Unfortunately the including cost. Regardless, Natular™ window of control is limited by the 30 day G30 will continue to be evaluated through release formulation of the product. more field trials in the 2017 season. Because of this characteristic, the application timing is critical to the ACKNOWELDGEMENTS effectiveness of the treatment. Within the 30 day spinosad release period following The author would like thank those application, the larvae must hatch and CMMCP staff members who helped become active enough to ingest sufficient identify, treat and monitor the sites used product for successful control. If the in this project, including former CMMCP Natular™ G30 is applied too early, eggs Field Biologist Todd B. Duval. present may not completely hatch out, and those larvae present may not have REFERENCES enough time to consume enough spinosad for adequate control. If the Andreadis TG, Thomas MC, Natular™ G30 was engineered for a Shepard JJ. 2005. Identification guide to longer release period the potential for the mosquitoes of Connecticut. Bulletin of the Connecticut Agricultural 12, 2016]. Experiment Station 966:1–173. http://www.cmmcp.org/spinosad.htm. CMMCP [Central Massachusetts Mosquito Control Project]. 2016. Extension Toxicology Network. Spinosad (Natular™) [Internet]. 1996. Methoxychlor. Extoxnet. Available Available from the Central from: Massachusetts Mosquito Control Project, http://extoxnet.orst.edu/pips/meth Northborough, MA [accessed December oxyc.htm

FIELD TRIALS OF NATULAR® G FOR CONTROL OF COQUILLETTIDIA PERTURBANS LARVAE IN SELECTED RETENTION PONDS FRANK H. CORNINE III MPH, & TIMOTHY D. DESCHAMPS Central Mass. Mosquito Control Project 111 Otis St. Northborough, MA 01532 (508) 393-3055 • [email protected] ABSTRACT

One of the primary pestiferous mosquitoes in the CMMCP service area is Coquillettidia perturbans, a mammal biting mosquito that develops in cattail habitats. Because Cq. perturbans larvae attach the aquatic roots of the cattails, traditional control methods can be less effective. Natular™ G, a relatively novel product, may be an option to address this situation. Spinosad is the active ingredient of Natular™ G, which is created from the fermentation of the naturally occurring soil organism Saccharopolyspora spinosa. To evaluate the potential of Natular™ G against Cq. perturbans, CMMCP conducted field trials in local retention ponds with a known history of this Eastern Equine Encephalitis and West Nile virus vector species.

INTRODUCTION hatch treatment, an option not available since the 1980’s. By the 1980’s the use of products such as methoxychlor (an organochlorine) by MATERIALS & METHODS CMMCP ceased, leaving no suitable pre- The retention ponds chosen for this hatch treatments. Natular™ G is a recent project have been monitored for several addition to the larvicide options available seasons. CDC traps baited solely with to CMMCP, and uses Spinosad as the compressed CO2 were used over this active ingredient. This Group 5 larvicide period to determine the abundance of can be used in several different mosquito Cq. perturbans. To gauge the viability of environments including temporary Natular™ G on this mosquito species, standing water, freshwater swamps, two of these retention ponds were storm water systems, and artificial designated as treatment sites, with two containers. Cattail marshes and others as non-treated controls. The freshwater emergent vegetation areas Natular™ G was applied in accordance are also included in these highlighted with the label at the two treatment application habitats, which makes this locations. Surveillance for Cq. product suitable for species such as Cq. perturbans began before emergence perturbans that develop in these specific began and continued through the season environments. Cq. perturbans are using the same CDC trap configuration unique because as larvae they attach that had been used in prior surveillance. themselves to the root systems of these Collections from these treatment and plants to breathe. CMMCP sought to non-treatment sites could be compared evaluate Natular™ G for use as a pre- to one another as well as the past data from those specific retention ponds to stage the Natular™ G would not determine the level of control achieved successfully control the mosquitoes. from the Natular™ G applications. CONCLUSION The data analysis from this surveillance The adult mosquito surveillance is not indicative of significant control. conducted around these select retention This reflection may be influenced by the ponds provided results that do not collection method rather than actual level indicate successful control. It is of control achieved by the Natular™ G on proposed that the finding is due primary the Cq. perturbans population. Although to faults with the surveillance practice of the CDC surveillance traps are using free standing CDC traps to collect positioned in close proximity to the emerging mosquitoes. To address this retention ponds, they do not exclude issue, CMMCP has begun developing adult Cq. perturbans that may have stationary emergence traps that will potentially migrated from outside collect adult Cq. perturbans directly from sources, from entering the collection the retention pond vegetation mats. This chamber. The applications of Natular™ will remove the possibility of non-native G may have been timed right, and in mosquitoes skewing the trial collections. adequate amounts to control the Cq. These traps will also allow for separate perturbans larvae, but if non-native well defined collections within the same adults entered the surveillance traps, the retention pond. Potentially, this could results would not have reflected the create an experimental design where a control success. particular retention pond has designated If the CDC traps collected only native Cq. treated and non-treated areas. perturbans from the selected retention Emergence traps could then be ponds, the results may be been caused established in both section types, by ineffective Natular™ G, improper reducing the previous bias between treatment schedule, or inadequate different treatment/non-treatment application. Although unlikely, the retention ponds. CMMCP will particular batch of product CMMCP incorporate this surveillance change into utilized in the trials could have been less future evaluations of Natular™ G and potent than advertised, which would have similar products as pre-hatch control contributed to the perceived low level of options for Cq. perturbans. control. Another potential issue with the ACKNOWLEDGMENTS product could have been associated with the timing of the application. As The authors would like to thank the Spinosad needs to be ingested for it to be CMMCP Commission, and past research effective, if the Cq. perturbans population assistants who helped with this project. was at the late 4th instar larval or pupal

BOTTLE ASSAYS OF FIELD COLLECTED MOSQUITOES FOR LEVEL OF RESISTANCE TO ZENIVEX® E20 IN CENTRAL MASSACHUSETTS 2016

FRANK H. CORNINE III MPH

Central Mass. Mosquito Control Project 111 Otis Street Northborough, MA 01532 (508) 393-3055 • www.cmmcp.org • [email protected]

ABSTRACT

In 2016 the Central Massachusetts Mosquito Control Project conducted bottles assays to establish a diagnostic baseline concentration for etofenprox resistance on the local mosquito population. The process is outlined by the Center for Disease Control and Prevention, with CMMCP initially using naïve field collected adult mosquitoes to create the standard concentration for which all susceptible mosquitoes would be tested against. Resistance to etofenprox was examined because the primary adulticide product of CMMCP is Zenivex® E20, of which etofenprox is the active ingredient. Through these bottle assays, the baseline concentration for etofenprox resistance was determined to be 18µg/ml for mosquitoes of the CMMCP service area and can now be utilized in future seasons to monitor the local resistance.

INTRODUCTION Although examples of pesticide resistance has been well documented, Pesticide resistance in this age of vector- the scope of the issue and its real world borne disease can hamper the ability of impact on public health control activities public health officials to successfully is not known. This is partially due to control threats. Potential resistance may varying levels of resistance surveillance also lead to the reemergence of several programs that currently exist. This factor diseases that would have been otherwise is shown in Massachusetts where some contained through control measures organized mosquito control agencies (Brogdon 1998). Current resistance in conduct zero resistance surveillance, select mosquito populations may be the while others have limited to well- result of historical insecticide use in the developed programs collecting data on agricultural and pest control industries pesticide resistance in mosquitoes. In (Rodriguez 2005). The bygone use of addition to the variety of resistance DDT for example, could have contributed surveillance programs, resistance also to current resistance to synthetic appears to be quite localized which pyrethroids, due to the mechanism for further clouds the impact. One noted resistance being similar for both example involved two separate mosquito (Brogdon 1998; McAbee 2003). Another populations that not only differed in associated and contributing factor may resistance levels, but also resistance be the contracting classes and options mechanism, all despite being only a few for public health insecticides as well as miles apart (Brogdon 1998). These growing regulatory restrictions (Brogdon continuing uncertainties surrounding 1998). insecticide resistance have supported CMMCP efforts to create baseline data and monitor for early resistance (Wheaton Science Products, Millville, detection. In the case of observed NJ) with various 1ml dilutions of the resistance, adulticide protocols could be product being analyzed. The solutions modified to ensure continued efficacy. used in this project were created using pesticide grade acetone (Thermo Fisher The primary adulticide product used by Scientific, Inc., Fair Lawn, NJ) and CMMCP during the 2017 season was undiluted Zenivex® E20, finishing at Zenivex® E20 (Wellmark International, etofenprox concentrations of 3µg/ml, Schaumburg, IL) (EPA Reg No. 2724- 6µg/ml, 12µg/ml, 18µg/ml, and 24µg/ml. 791), a synthetic pyrethroid that utilizes In addition to the bottles coated with the active ingredient etofenprox. Prior to etofenprox, untreated bottles were this season CMMCP had used another created using only the pesticide grade synthetic pyrethroid, ANVIL® 10+10 acetone to establish a control for the (Clarke Mosquito Control Products, Inc., assays. Roselle, IL) (EPA Reg. No. 1021-1688- 8329). Unlike Zenivex® E20, ANVIL® For the baseline concentration to be 10+10 uses the active ingredient determined, it is important that the sumithrin along with the synergist mosquito specimens used have not been piperonyl butoxide (PBO). The absence unexposed to synthetic pyrethroids, of PBO in Zenivex® E20 is one of its whether through the CMMCP program or perceived benefits over ANVIL® 10+10. other activities. To meet this criteria, As this was the first season CMMCP CDC light traps (John W. Hock Co., primarily used Zenivex® E20, a Gainesville, FL) were deployed in diagnostic baseline concentration for wetlands surrounding a local organic etofenprox needed to be established, farm property. This location has been which was the goal of the 2017 bottle identified and treated as a pesticide assays. exclusion since 2006. Once the labeled bottles were coated and sufficiently dried, MATERIALS & METHODS approximately 10-15 adult mosquitoes were aspirated into each bottle The procedure used for these bottle mechanically. The CDC light traps used assays comes from the Centers for compressed carbon dioxide gas as an Disease Control and Prevention (CDC attractant at a release rate of 500cc/min. 2010). A reference, or baseline, ABC standard collection nets (Clarke concentration needs to be determined by Mosquito Control Products, Inc., Roselle, using adult mosquitoes that originate IL) were used in conjunction with the from an area that has been excluded CDC light traps and held the mosquitoes from pesticide exposure, CMMCP or until introduction into the assay bottles. otherwise. Using the diagnostic information established from these naïve With these local unexposed mosquitoes specimens against mosquito populations collected, coated bottles created, and from the CMMCP service area, one can mosquitoes aspirated into the bottles, gauge if resistance has developed and to specimen knockdown percentage was what degree. Determining the baseline recorded at 5 minute intervals, up to concentration for bottle assays begins by 100% knockdown. For the untreated lining clean 250ml Wheaton bottles control bottles lined with only acetone (zero Zenivex® E20), knockdown baseline group, it could be an indication percentage was observed at 5 minute of resistance developing. intervals for up to two hours. Each one of the diluted etofenprox solutions RESULTS underwent several trials until a concentration was found that produced To determine the baseline concentration morality curve around 30 minutes for total for etofenprox resistance in local knockdown. This baseline mosquito populations, concentrations of concentration, once determined, could 3µg/ml, 6µg/ml, 12µg/ml, 18µg/ml, and eventually be used against the exposed 24µg/ml were tested. The diagnostic mosquito populations, along with baseline concentration of Zenivex® E20 untreated control bottles running was determined to be 18µg/ml. The concurrently. Potential differences other concentrations of etofenprox between the plotted knockdown curves of produced either too fast or too slow the treatment mosquito populations and mortality curves (Figure 1). Future bottle the original baseline group could be used assays will be conducted using only the to determine if resistance was forming in 18µg/ml etofenprox diagnostic local mosquitoes. If test specimens concentration. survived longer than those of the

Figure 1: Zenivex® E20 Concentration Knockdown Curves for Baseline Determination

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3.00µg/ml 6.00µg/ml 12.00µg/ml 18.00µg/ml 24.00µg/ml

DISCUSSION was the first season where Zenivex® E20 was the primary adulticide used by It was important to determine the CMMCP. Prior to this year ANVIL® baseline concentration of etofenprox 10+10, with the active ingredient against local mosquito species, as this sumithrin and synergist piperonyl butoxide, had been the principal product diagnostic concentration for etofenprox used. Now that a diagnostic baseline determined this season will be utilized in concentration has been established resistance surveillance throughout the CMMCP can use it against field collected CMMCP service area in the 2017 mosquitoes from the CMMCP service season. area. As long as Zenivex® E20 is used as the primary adulticide product, REFERENCES resistance surveillance will continue using this baseline concentration against Brogdon WG, McAllister JC. 1998. local mosquito populations. When Insecticide Resistance and Vector CMMCP changes products, the Control. Emerg Infect Dis 4:605- resistance surveillance program will 613. make the appropriate shift. CDC. 2010. Guideline for evaluating The diagnostic baseline concentration for insecticide resistance in Zenivex® E20/etofenprox against local vectors using the CDC bottle mosquito populations was determined to bioassays. Atlanta, GA: Center for be 18µg/ml, but results were somewhat Disease Control and Prevention variable. This may have been a [accessed November 24, 2015]. byproduct of the mosquitoes being field Available from: collected opposed to lab reared. Adult http://www.cdc.gov/malaria/resourc mosquitoes collected from the field may es/pdf/fsp/ be at various metabolic stages and ir_manual/ir_cdc_bioassay_en.pdf process exposure to synthetic pyrethroids at different rates. If lab McAbee RD, Kang KD, Stanich MA, reared mosquitoes are used instead, Christiansen JA, Wheelock CE, their food source intake can be more Inman AD, Hammock BD, Cornel readily regulated and uniform for bottle AJ. 2003. Pyrethroid tolerance in assay testing. Another potential factor in Culex pipiens pipiens var molestus variability could have been in the from Marin County, California. Pest solutions used for determining the Manag Sci 60:359-368. baseline concentration. Zenivex® E20 product was used, but alternatively Rodriguez MM, Bisset JA, DeArmas Y, technical etofenprox could have been Ramos F. 2005. Pyrethroid diluted into solution, which may have Insecticide-Resistant Strain of been more stable and accurate over the Aedes Aegypti From Cuba Induced course of bottle assays. CMMCP will by Deltamethrin Selection. J Am investigate obtaining etofenprox and Mosq Control Assoc 21(4):437-445. comparing the two sources. The

CMMCP ZIKA MONITORING STRATEGY – 2016

TIMOTHY D. DESCHAMPS

Central Mass. Mosquito Control Project 111 Otis St. Northborough, MA 01532 (508) 393-3055 ● [email protected]

INTRODUCTION:

Zika Virus is an emerging arbovirus (flavivirus) causing outbreaks in Central, South and Latin America by Aedes mosquitoes, primarily Ae. aegypti. Ae. albopictus is also implicated in the spread of Zika Virus.

INTENT & PURPOSE:

To determine the extent of Ae. albopictus distribution in member CMMCP communities.

PRODUCTS & PROCEDURES:

To use ovicups lined with seed germination paper to collect mosquito eggs. Process these egg papers according to a protocol established by MDPH. MDPH will rear eggs to determine & species and report findings to CMMCP. If Ae. albopictus is identified, then supplemental ovicups as well as BG Sentinel traps will be deployed to the area to determine the extent of the distribution. CMMCP AERIAL MOSQUITO LARVAL CONTROL

PROGRAM

SPRING 2016

FRANK H. CORNINE III, TODD B. DUVAL & TIMOTHY D. DESCHAMPS

Central Mass. Mosquito Control Project 111 Otis Street Northborough, MA 01532 (508) 393-3055 • www.cmmcp.org

AERIAL MOSQUITO LARVAL CONTROL PROGRAM – SPRING 2016

FRANK H. CORNINE III, TODD B. DUVAL & TIMOTHY D. DESCHAMPS Central Mass. Mosquito Control Project 111 Otis Street Northborough, MA 01532 (508) 393-3055 • www.cmmcp.org • [email protected]

ABSTRACT

To reduce the volume of numerous early summer mosquito species, the Central Massachusetts Mosquito Control Project performed a focused aerial application of Bacillus thuringiensis israelensis in the member communities of Billerica, Boxborough, and Chelmsford. This event took place over the course of two consecutive days, the 20th and 21st of April 2016, covering approximately two thousand acres of wetlands inundated with mosquito larvae. Observation of these larvae conducted prior to and following the aerial larvicide, showed an average reduction of 86.36% for each surveillance site. This decrease in larvae will reduce the level of pestiferous, mammal-biting adult mosquitoes and lessen the need for ultra-low volume adulticiding during the summer months.

OBJECTIVE emergence and also diminish a potential vector of mosquito-borne disease. The primary mosquito species targeted in this aerial application were METHODS AND MATERIALS Ochlerotatus abserratus, Ochlerotatus excrucians, and Ochlerotatus VectoBac G® (EPA Reg. No. 73049-10) canadensis. The eggs of these is the formulation of Bacillus mosquitoes overwinter in the temporary thuringiensis israelensis used in this snow pools, and upon spring snow melt, aerial larvicide program, as well as the will hatch into larvae and begin to CMMCP ground larvicide program develop. Ochlerotatus abserratus and (CMMCP 2016). Originally registered in Ochlerotatus excrucians are both 1961, Bacillus thuringiensis is a univoltine species, meaning they only naturally occurring bacterium that have one generation per season, with produces target specific toxins when any eggs laid by adult female ingested. This asexually reproducing mosquitoes not hatching until the soil bacterium produces spores, as well following year. Conversely, as crystalline byproducts. It are these Ochlerotatus canadensis is considered crystals which interfere with the a multivoltine mosquito species that has digestion of the targeted species, the ability to produce more than one eventually leading to mortality. Bacillus generation a season. This particular thuringiensis readily breaks down when species has also shown the ability to applied into the environment, and poses harbor mosquito-borne diseases such no threats to groundwater (Extension as West Nile virus and Eastern Equine Toxicology Network 1996; National Encephalitis (Andreadis 2005). If Pesticide Information Center 2015). successful, this type of aerial larvicide will reduce the need for adulticiding North Fork Helicopter (Cutchogue, New these pestiferous species upon York) was contracted once again to facilitate the application of this aerial larvicide program. The Billerica and the basis for determining the level of Chelmsford portions of the program control achieved for the aerial larvicide were conducted on April 20th, with program. Sampled larvae are always Boxborough the following day. Warren returned to ensure that the treatment Farm in Chelmsford was utilized as the and control observations are not staging area on the first application day, artificially impacted, however, larvae are with Minute Man Airfield in Stow being retrieved from non-flagged locations in used for the second and final day. each RDS to obtain information on the CMMCP personnel identified specific mosquito species present at the time of wetlands bearing mosquito larvae, application. Following the larvicide which North Fork Helicopter was then event, the presence or absence of able to target and treat with VectoBac VectoBac G® is also noted in addition to G®. Approximately two thousand total the larval density (Massachusetts acres were treated in this program, or Department of Agricultural Resources 600, 520, and 880 acres between 1998). As per 333CMR 13.04 (7) a Billerica, Chelmsford and Boxborough legal notification of the aerial larvicide respectively. This aerial larvicide was placed in The Boston Globe on program utilized a 5lbs/acre application February 10th, 2016, and also posted rate. Historically this application rate of on the CMMCP website VectoBac G® has provided proper (http://www.cmmcp.org/) (Appendix A). control in these areas. RESULTS To evaluate the efficacy of this and other aerial larvicide events in the On average, the treatment RDS of Commonwealth of Massachusetts, the Billerica, Boxborough, and Chelmsford Generic Environmental Impact Report experienced an 86.36% reduction in (GEIR) outlines the formation of larvae following the 2016 spring aerial recoverable dip stations (RDS). For larvicide. Individually, the Billerica RDS each town involved in the aerial larvicide exhibited an average reduction of program, one RDS is needed for every 88.94%, while an average reduction of 250 acres treated as well as one control 80.28% and 91.88% was observed for RDS located in an untreated area. Ten Boxborough and Chelmsford flagged locations at each RDS are respectively. The untreated (control) sampled for larvae, before treatment, at RDS of the three communities averaged 24 hours post application and 48 hours a 7.32% decrease in larvae from the post application. Larval density beginning of the program (Table 1; changes among these observations are Figures 1-3).

Table 1: Larval Surveillance of Treatment and Control RDS Treatment Sites Pre-application Post-application Observed Change BIL116 71 7 -90.14% BIL112 56 1 -98.21% BIL408 65 14 -78.46% BOX128 23 0 -100.00% BOX118 30 7 -76.67% BOX92 24 0 -100.00% BOX121 27 15 -44.44% CHM82 53 2 -96.23% CHM279 44 7 -84.09% CHM236 64 3 -95.31% Control Sites Pre-application Post-application Observed Change BIL227 50 62 24.00% ACT37 62 58 -6.45% CHM146 124 75 -39.52%

Figure 1: Billerica Treatment RDS Results Pre- and Post Application

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Figure 2: Boxborough Treatment RDS Results Pre- and Post Application

Figure 3: Chelmsford Treatment RDS Results Pre- and Post Application

DISCUSSION canadensis mosquito larvae, the residents of Billerica, Boxborough, and Every spring, upon the detection of early Chelmsford will experience diminished season mosquito larvae, CMMCP field pressure from the early summer adults technicians begin to apply Bacillus of these three mosquito species. thuringiensis israelensis to reduce the Additionally, the need for adulticiding in eventual emergence of adults. For these areas will be lessened because of locations in Billerica, Boxborough, and the control achieved in this program. Chelmsford that are too massive and CMMCP will incorporate this years’ inundated to larvicide by hand, CMMCP experience into the design and may opt for treatment through the aerial implementation of future aerial larvicides larvicide program. In 2016, this events. This includes the potential application event occurred over two expansion of the program into additional days, April 20th and 21st. Larval CMMCP member communities. surveillance was conducted prior to the aerial larvicide, and twice following the ACKNOWLEDGEMENTS treatment. With an average larval reduction of 86.36%, the 2016 CMMCP The authors would like to acknowledge spring aerial experienced measured the participation of Billerica, success. Conversely, the untreated Boxborough, and Chelmsford in this control sites had an average reduction supplemental program; North Fork of only 7.32%, which is consistent with Helicopters for providing the helicopter previous CMMCP aerial larvicide services; Warren Farm, Chelmsford and events. Minute Man Airfield, Stow for providing loading zones; the CMMCP Through the observed reduction of Oc. Commission, and the CMMCP staff for abserratus, Oc. excrucians and Oc. larval monitoring, larval identification, site selection, map development and assisting with the helicopter application. Extension Toxicology Network. 1996. An additional thanks goes to Nate Bacillus thuringiensis. Extoxnet. Boonisar of the Norfolk County Mosquito Available from: Control District for his assistance once http://extoxnet.orst.edu/pips/bacill again with creating target files for the us.htm helicopter navigation system. Massachusetts Department of REFERENCES Agricultural Resources. 1998. Generic Environmental Andreadis TG, Thomas MC, Shepard Impact Report (GEIR). JJ. 2005. Identification guide to Massachusetts Department of the mosquitoes of Agricultural Resources. Available Connecticut. Bulletin of the from: Connecticut Agricultural http://www.mass.gov/eea/docs/ag Experiment Station 966:1–173. r/mosquitos/geir-docs/geir-full- text.pdf CMMCP [Central Massachusetts Mosquito Control Project]. 2016. National Pesticide Information Center. Bti (Bacillus 2015. Bacillus thuringiensis: thuringiensis var. israelensis). General Fact Sheet. Northborough, MA: Central Mass. National Pesticide Information Mosquito Control Project. Center. Available from Available from: http://npic.orst.edu/factsheets/BT http://www.cmmcp.org/bti.htm gen.pdf

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Town of Billerica: Ü LOWELL Aerial Larvicide Program 2016

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© copyright 2017 ♦ CMMCP

INTRODUCTION

Member residents request assistance from the menu of services offered to them by CMMCP. Requests for adulticiding (spraying) and larval control are the most common forms of service requests we receive. We accepts these requests through a variety of means, primarily by telephone, but increasing more by the online service request form from the CMMCP website. Additional methods include personal visits to our office, phone calls on behalf of residents from town and/or state officials, and direct requests to our field staff. The CMMCP Commission requested a survey of resident who received service in 2016 to determine if our staff was meeting acceptable levels of customer satisfaction. This is the same survey that was done in 2005-2015 (excluding 2006). After compiling these results, we find that a majority of residents in our service area were satisfied with our control efforts and methods, which mirrors our results from previous years.

SURVEY METHODOLOGY

In 2016 we received 16,294 requests for service, ranging from adulticiding to larval control. 10,984 adulticiding calls were filtered (multiples removed) and placed into a separate database. Service calls were sorted according to town, and each town was tabulated for total requests received in 2016. These towns were then graphed to show which towns had the most calls. Each town was assigned a percentage according to this data. This percentage would determine the number of postcards sent to each town from the overall total. The CMMCP Commission has determined that 1,500 postcards would be a representative sample of the service calls received this year (this is an increase of 500 over the first 3 surveys but the same as 2009-2015). The survey was designed to be as easy as possible for residents to access and complete. An online survey was created through SurveyMonkey®, and the postcards would include unique identifiers that the residents would use. The postcards contained a blind weblink to the survey so that uninvited users would not be able to participate in the survey. Information such as how they contacted us, were the office and field staff helpful and informative, how long did they wait for service, was the service provided effective, and their overall satisfaction was measured. This study uses the same methodology as all previous resident surveys.

From 1,500 postcards mailed, 115 responses were received (7.6%). The results are outlined in this report.

TIMOTHY D. DESCHAMPS, Executive Director Central Mass. Mosquito Control Project 111 Otis Street Northborough, Massachusetts 01532 www.cmmcp.org ♦ [email protected]

2 1). In your most recent experience, how did you contact the Central Mass. Mosquito Control Project?

Number Percent Telephone 34 29.82% Website 78 68.42% In person 1 0.88% Other 1 0.88% Total 114

Comments: the website outpaces the phone system as the most popular method of reaching our staff.

2). If by telephone or in person at the CMMCP office, were your questions or concerns answered to your satisfaction?

Number Percent Yes 39 100% No 0 0% Total 39

Comments: communication from the operators of the telephone system is clear and effective.

3 3). If by telephone, did you experience difficulty reaching our staff?

Number Percent Yes 3 7.5% No 37 92.5% Total 40

Comments: over 90% of residents polled did not experience any problems reaching our staff through the current system.

4). If through the website or e-mail, did you find the information you needed in a satisfactory manner?

Number Percent Yes 92 97.84% No 2 2.13% Total 94

Comments: nearly all respondents found the information they required on the website without difficulty.

4 5). Please give the approximate time you waited for service from your initial request:

Number Percent

1-3 days 29 26.85%

3-5 days 21 19.44%

1 week 34 31.48%

2 weeks+ 24 22.22%

Total 108

Comments: 77.78% were serviced within one week or less

6). Did you find our response from your initial request to when you received service within a reasonable amount of time?

Number Percent Yes 101 91.82% No 9 8.18% Total 110

Comments: a majority thought that the response time was reasonable.

5 7). When you received service, did our field representative appear knowledgeable and competent about his/her profession?

Number Percent Yes 91 94.79% No 5 5.21% Total 96

Comments: Our staff projects a positive and professional image to the public. Of the respondents that said no, many stated they did not speak to a rep. from CMMCP.

8). Were your questions and concerns answered by the Technician to your satisfaction?

Number Percent Yes 88 94.62% No 5 5.38% Total 93

Comments: most residents polled thought our Technicians answered their questions to their satisfaction.

9). Did you receive any written information (pamphlets, etc.) from our representative?

Number Percent Yes 86 79.63% No 22 20.37% Total 108

Comments: more residents are receiving our written information and this number has improved or held steady from year to year.

6 10). Did you find this information useful?

Number Percent Yes 84 88.42% No 11 11.58% Total 95

Comments: our written educational materials are useful to residents when they receive it.

11). Did you request service more than once in 2016?

Number Percent Yes 54 47.79% No 59 52.21% Total 113

Comments: just under half of our service calls are repeat calls according to the residents polled.

12). If you requested additional service in 2016, was it because the original application was insufficient to meet your needs, or for a later re-treatment or follow up?

Number Percent Retreatment 44 75.86% Insufficient 14 24.14% Total 58

Comments: 3/4 of our repeat calls are for additional service, not because the first application didn’t meet their needs.

7 13). Would you/did you recommend our service to others in the future?

Number Percent Yes 109 98.2% No 2 1.8% Total 111

Comments: all residents polled but two would recommend our services to others.

14). In your opinion, did our application made your area better, worse, or had no effect?

Number Percent Better 100 88.5% Worse 0 0% No Effect 13 11.5% Total 113

Comments: nearly 90% of all residents received relief from mosquitoes from our program.

8 15). If you think your area improved, can you give an approximate length of time you experienced relief from mosquito annoyance?

Number Percent 1-3 days 16 16.16% 3-5 days 13 13.13% 1 week 23 23.23% 2 weeks+ 47 47.47% Total 99

Comments: over 2/3 of residents reported relief of 1 week or more, over half report greater than 2 weeks of relief.

16). On average, our services cost $2.00 – $4.00 per person each year (withheld from local aid rec’d from the State). In your opinion, is this amount too high, too low, or sufficient?

Number Percent Sufficient 93 83.04% Too Low 18 16.07% Too High 1 0.89% Total 112

Comments: most residents are satisfied with the assessments paid from local aid for our services.

17). In which month or months do you recall receiving service?

Number Percent May 9 8.33% June 39 36.11% July 22 20.37% August 5 4.63% More than 1 33 30.56% Total 108

9 18). Overall, are you happy with the service provided this year by CMMCP?

Number Percent Yes 101 88.6% No 12 11.4% Total 114

Comments: almost 9 out of 10 residents were happy with the services provided by CMMCP in 2016.

19). Do you plan on using our service again in the future?

Number Percent Yes 112 98.25% No 2 1.75% Total 114

10 Please rate our performance for 2016 from 0 to 5, where 5 is the best rating, 0 is the worst rating:

A. The information you received over the phone was informative & helpful: 186 points from 215 (43 respondents) – 4.33 average from 5

B. The information on our website is easily available and helpful: 418 points from 495 (99 respondents) – 4.24 average from 5

C. The response time for service is reasonable: 455 points out of 560 (112 respondents) – 4.06 average from 5

D. Our field staff that responded is knowledgeable and competent: 437 points out of 485 (97 respondents) – 4.51 average from 5

E. The service provided was effective: 451 points out of 565 (113 respondents) – 3.99 average from 5

F. This service is reasonable compared to the cost: 406 points out of 445 (89 respondents) – 4.56 average from 5

G. Please rate your overall satisfaction with the service received in 2016: 493 points out of 565 (113 respondents) – 4.36 average from 5

Total satisfaction rating: 2,846 points out of 3,330 possible – 4.29 average

666 total responses to questions

11 CONCLUSION

Overall satisfaction was 88.6%, and 98.25% would use our services again in the future. Answer to question #9 shows a steady increase over past years in regards to residents receiving public relations materials. Overall this survey shows high satisfaction amongst the respondents, with some variability in some ratings than in past surveys.

Reprints of this document are available by calling our office at (508) 393-3055 or sending an e-mail to [email protected]. This survey has been included in our 2016 annual report available to residents and town officials in all cities and towns in our service area, as well as members of the State Reclamation & Mosquito Control Board. This has also been posted on our website on the “Research and Efficacy” link (from the “Our Services” page).

The author would like to thank the staff at CMMCP and the CMMCP Commission, and especially the residents and public officials in the member cities and towns we provided service to in 2016.

12 SOURCE REDUCTION/TIRE RECYCLING REPORT 2016

For Earth Day 2010, CMMCP officially announced a tire recycling program added as a value added service to our member cities and towns. This program operates under grant monies received and the CMMCP operating budget. Tire piles provide suitable areas for larval mosquito development, including those species known to carry West Nile virus. During the course of one season, the potential exists for hundreds or even thousands of mosquitoes to emerge from just one tire. If tires infested with mosquito eggs, larvae or pupae are transported, the potential to introduce mosquito species into new areas and/or the potential for the spread of arboviruses and their transmission may increase significantly. For these reasons and as a value added service to our member cites and towns, CMMCP has developed a used tire program, consisting of the following guidelines:

 We accept passenger and light truck tires only  The maximum number tires from one property will be 10 at one time, subject to change without notice  Requests for tire removal shall be done according to established procedures  We reserve the right to refuse anything determined to be unsuitable for this program

Tires accepted as part of this program are sent to an approved facility for recycling or disposal. This program is subject to end without notice.

As a focus of this program in 2016 to minimize Ae. albopictus (Asian Tiger mosquito) and other container-species larval habitat, we have been removing tire piles in member cities and towns that we have documented in our files. If you know of a tire pile in your area, or would like to participate in a curbside pickup in the future, please send the following information to [email protected]; NAME, ADDRESS, TOWN, PHONE, E-MAIL, # of TIRES (off the rim), LOCATION OF TIRES, ANY COMMENTS. When we schedule a curbside pickup event in your area you will be notified in advance.

ELIGIBILITY: to qualify for this program you must be a resident or municipal official in a CMMCP member city or town and the tires must be in or from that locality. Businesses are not eligible at this time. COST: there is no additional cost to residents or municipalities; this program is part of the full suite of mosquito control services offered.

2016 Tire Collection Data: In 2016 CMMCP collected and recycled 48.31 tons of tires. Our staff was able to eliminate over 20 tons of abandoned tires located throughout the district. This is important as it eliminates established larval mosquito habitat and the need for repeated pesticide applications. This effort saw CMMCP work in conjunction with the Boy Scouts of America on an Eagle Scout project in Hopkinton and landowners from the Sudbury Valley Trustees (SVT). Elimination of abandoned tire sites accounted for over 40% of CMMCP’s recycling efforts during 2016. Approximately 45% of the tires recycled by CMMCP originated from tire recycling events coordinated by our member communities. The remainder of the recycling efforts originated from residential tire removal called into our office from member residents. Since the inception of this tire program in 2010 CMMCP has recycled over 220 tons of tires. Over the past 6 years the CMMCP Tire Program has received several awards and citations including the Environmental Merit Award from the US EPA and the Institution and Nonprofit Award from MassRecycle.

Respectfully submitted,

Timothy E. McGlinchy Director of Operations

2016 TOWN TIRE COLLECTION DATA:

Town Tires collected Town Tires collected Acton 19 Littleton 8 Ashland 22 Lowell 42 Auburn 83 Lunenburg 762 Billerica 188 Milford 75 Boxboro 6 Millbury 186 Boylston 320 Natick 39 Chelmsford 21 Northboro 97 Clinton 206 Northbridge 5 Dracut 59 Shrewsbury 32 Fitchburg 345 Stow 21 Gardner 306 Sturbridge 273 Holliston 2 Tewksbury 211 Hopkinton 802 Webster 18 Hudson 77 Westboro 2 Lancaster 257 Westford 71 Leominster 274 Wilmington 2

2016 EVENT TOTALS:

Residential 612 6.12 tons

Roadside 120 1.2 tons

Town Recycle Events 2,197 21.97 tons

Large Tire piles 1,902 19.02 tons

Total for 2016 4,831 48.31 tons

Total to Date: (2010-2016) 22,011 tires 220.11 tons

TOWN OF AUBURN

DATE WORK DONE LOCATION

01-04-16 Brush Cutting 300’ Rochdale Street Stream Cleaning 400’ Rochdale Street 01-19-16 Administrative Contact Board of Health 03-28-16 Stream Cleaning 50’ Stonebridge Road Stream Cleaning 10’ Forest Park Drive Stream Cleaning 40’ Forest Park Drive Stream Cleaning 10’ Church Street Stream Cleaning 45’ Oxford Street North Stream Cleaning 65’ Leicester Street Stream Cleaning 5’ Leicester Street Stream Cleaning 10’ Leicester Street Stream Cleaning 110’ Leicester Street Stream Cleaning 205’ Anderson Way Stream Cleaning 25’ Leicester Street Stream Cleaning 100’ Rochdale Street Culvert Cleaning (12) Stonebridge Road, Forest Park Drive, Church Street, Oxford Street North, Leicester Street, Anderson Way, Rochdale Street 03-31-16 Larviciding Washington Street, Bancroft Street, Millbury Street, Jade Hill Road, Technology Drive, Coolidge Street, Santom Street, Jacobs Way 04-05-16 Administrative Contact Board of Health, Town Clerk 04-12-16 Stream Cleaning 309’ South Street Stream Cleaning 60’ Elizabeth Street Stream Cleaning 10’ Elizabeth Street Stream Cleaning 85’ Barnes Street Stream Cleaning 10’ Barnes Street Stream Cleaning 20’ South Street Stream Cleaning 25’ South Street Stream Cleaning 5’ Oxford Street Stream Cleaning 20’ Oxford Street Stream Cleaning 65’ Oxford Street Stream Cleaning 10’ Oxford Street Stream Cleaning 15’ Oxford Street Culvert Cleaning (14) South Street, Elizabeth Street, Barnes Street, Oxford Street 04-13-16 Larviciding Leicester Street, Rochdale Avenue, Dale Avenue Larviciding Re-check Washington Street Larval Survey Elizabeth Street, Leicester Street, Anderson Way, Drake Court, Rochdale Street, Dale Avenue, John Williams Drive 04-22-16 Public Relations Walsh Avenue Larviciding Walsh Avenue, Bryn Mawr Avenue Larval Survey Walsh Avenue, Pheasant Court, Holstrom Court, Southbridge Court, Sword Street, Pakachoag Street, Forest Park, Pakachoag Crossing, Bryn Mawr Avenue, Leicester Street, Rochdale Street 04-29-16 Larviciding South Street, Cedar Street, Oxford Street South Larval Survey Walsh Avenue, South Street, Cedar Street, Oxford Street South, Coachman Lane 05-06-16 Larviciding Maple Street, Tinker Hill Road, Blaker Street Larval Survey South Street, Maple Street, Tinker Hill Road Blaker Street, Albert Street, Southbridge Street, Masonic Circle 05-07-16 Source Reduction Oxford Street North – Daniel Horgan Skating Arena Tire Removal [82] 05-13-16 Public Relations Laurel Lane, Coolidge Street Larviciding Laurel Lane, Pinrock Road Larval Survey Maple Street, Coolidge Street, Laurel Lane, Tuck Farm Road, Westec Drive, Pinrock Road 05-16-16 Public Relations Pakachoag Elementary School – Mosquito Program TOWN OF AUBURN

DATE WORK DONE LOCATION

05-19-16 Public Relations Bryn Mawr Elementary School – Mosquito Program 05-20-16 Catch Basin Larviciding Sword Street, Franklin Street, Woodland Road, Rockaway [190] Road, Alpine Trail, Shore Drive, Forest Drive, Pineview Trail, A Street, B Street, C Street, Sumner Street, Adella Street, Shirley Street, Chestnut Avenue, White Terrace, June Street, Wellman Street, Clark Street, Mahlert Court, Hanna Drive, Gwen Drive, Heard Street, Dixon Avenue, Duncan Street, Carroll Street, Gates Court, Lincoln Street, Marilyn Drive, Mohawk Avenue, Pinehurst Avenue, Burnett Street, Magna Vista Drive, Rochelle Street, Silver Street, Holmes Street, Old Colony Road, Horseshoe Drive, Westwood Drive, Courville Road, Karen Avenue, John Williams Drive, Sara Drive Lorna Drive, Dale Avenue, Cemetery Road, Camper Hill Road, Elmwood Road, Sunrise Avenue, Idlewood Drive, Maria Avenue, Colonial Road, Oakwood Avenue 05-23-16 Catch Basin Larviciding Erica Lane, Malvern Road, Matnick Circle, Hoover Road, [298] Alex Circle, Springbrook Road, Summer Hill Road, Manor Road, Lesley Avenue, Davis Road, Thayer Avenue, Lexington Avenue, Ward Street, Upland Street, Old Farm Road, Meadowbrook Road, Auburn Hill Road, Saybrook Way, Curtis Street, Betty Street, Stone Bridge Road, Southold Road, Pickering Street, Hillside Street, Center Street, Arlington Street, Amesbury Street, Elmwood Street, Gibson Road, High Ridge Road, Preston Street, Field Street, Newton Street, Willis Street, Jerome Avenue, Aurilla Court, Warwick Street, Glenview Road, Exeter Drive, Highland Drive, Hilton Avenue, Cutting Avenue, Wyoma Drive, Clarendon Road, Kimball Road, Forest Park Drive, Pakachoag Street Set Up Trap Crowl Hill Road, Athens Street 05-24-16 Pick Up Trap Crowl Hill Road, Athens Street 05-27-16 Public Relations Goddard Drive – Senior Center 05-31-16 Set Up Trap Central Street, Perry Place 06-01-16 Pick Up Trap Central Street, Perry Place 06-06-16 Set Up Trap Rochdale Street, Anderson Way 06-07-16 Administrative Contact Police Department Public Relations Hilltop Farm Road, Booth Road, Fenwick Circle, Prospect Street, Kelly Street, Appleton Road, Phoenix Road, Bridle Path, Sunnyside Road, Hill Street, West Street, Warren Road, Sherman Avenue, Elbridge Road, Waterman Road, Pioneer Lane, Goulding Drive, Linda Avenue, Melba Avenue, Oxford Street Pick Up Trap Rochdale Street, Anderson Way 06-10-16 Administrative Contact Police Department Public Relations Warwick Street, Malvern Road, Hampton Street, Jerome Avenue, Field Street, Preston Avenue, Elmwood Street, Eaton Avenue, Vesper Street, Clarendon Road, Walsh Avenue, Pheasant Court, Burnap Lane, Harrison Avenue, Westlund Avenue, Pakachoag Street, Wyoma Drive, Glen View Drive, Amesbury Street, Arlington Street, Erica Lane, Bryn Mawr Avenue, Regis Drive, Wentworth Drive, Briarcliff Drive, Amherst Street, Mayfield Road, Old Cart Road, Inwood Road, Sherwood Road, Ashcroft Street, Berlin Street, Bylund Avenue, Dale Avenue, Lorna Drive, Sara Drive, Burnett Street, Silver Street, Horseshoe Drive, Westwood Drive, Magna Vista Drive Adulticiding Warwick Street, Malvern Road, Hampton Street, Jerome Avenue, Field Street, Preston Avenue, Elmwood Street, Eaton Avenue, Vesper Street, Clarendon Road, Walsh Avenue, Pheasant Court, Burnap Lane, Harrison Avenue, TOWN OF AUBURN

DATE WORK DONE LOCATION

Westlund Avenue, Pakachoag Street, Wyoma Drive, Glen View Drive, Amesbury Street, Arlington Street, Erica Lane, Bryn Mawr Avenue, Regis Drive, Wentworth Drive, Briarcliff Drive, Amherst Street, Mayfield Road, Old Cart Road, Inwood Road, Sherwood Road, Ashcroft Street, Berlin Street, Bylund Avenue, Dale Avenue, Lorna Drive, Sara Drive, Burnett Street, Silver Street, Horseshoe Drive, Westwood Drive, Magna Vista Drive 06-13-16 Set Up Trap Central Street, Perry Place 06-14-16 Administrative Contact Police Department Public Relations White Terrace, Rochdale Street, Chestnut Avenue, Clark Street, June Street, Adella Street, Sumner Street, Shirley Street, Boyce Street, Sword Street, Woodland Road, Alpine Trail, Snare Drive, Rockland Road, Idlewood Drive, Colonial Road, Maria Avenue, Oakwood Avenue, Rockland Road, Rockland Road Court, Highland Street, Perry Street, Jade Hill Road, Montclair Drive, Millbury Street, Old Common Road, Riverside Drive, Camden Drive, Elm Street, Stone Street, Grove Street, School Street, Pleasant Street, Winchester Avenue, Westchester Drive, Barbara Avenue, Marion Avenue, South Street, Central Street, Coolidge Street, South Street, Carriage Drive, Santom Street Adulticiding White Terrace, Rochdale Street, Chestnut Avenue, Clark Street, June Street, Adella Street, Sumner Street, Shirley Street, Boyce Street, Sword Street, Woodland Road, Alpine Trail, Snare Drive, Rockland Road, Idlewood Drive, Colonial Road, Maria Avenue, Oakwood Avenue, Rockland Road, Rockland Road Court, Highland Street, Perry Street, Shore Drive, Maria Avenue, Jade Hill Road, Montclair Drive, Millbury Street, Old Common Road, Riverside Drive, Camden Drive, Elm Street, Stone Street, Grove Street, School Street, Pleasant Street, Winchester Avenue, Westchester Drive, Barbara Avenue, Marion Avenue, South Street, Central Street, Coolidge Street, South Street, Carriage Drive, Santom Street, Old Meeting House Road Pick Up Trap Central Street, Perry Place 06-20-16 Set Up Trap Rochdale Street, Anderson Way, Heard Street 06-21-16 Administrative Contact Police Department Public Relations Oxford Street North, Otis Street, Zabelle Avenue, Rochdale Street, Wallace Avenue, Burnett Street, Lorna Drive, Leicester Street, Crowl Hill Road, Merriam Street, Briarcliff Road, Bryn Mawr Avenue, Murray Avenue, Inwood Drive, Pinrock Road, Laurel Lane, Jacobs Way, Barnes Street, Deer Track Drive, Elizabeth Drive, Paul Revere Road, Gilbert Way, Cedar Street, Oxford Street South, Coachman Lane, Tinker Hill Road Adulticiding Oxford Street North, Otis Street, Zabelle Avenue, Rochdale Street, Wallace Avenue, Burnett Street, Lorna Drive, Leicester Street, Crowl Hill Road, Merriam Street, Briarcliff Road, Bryn Mawr Avenue, Murray Avenue, Inwood Drive, Pinrock Road, Laurel Lane, Jacobs Way, Barnes Street, Deer Track Drive, Elizabeth Drive, Paul Revere Road, Gilbert Way, Cedar Street, Oxford Street South, Coachman Lane, Tinker Hill Road Pick Up Trap Rochdale Street, Anderson Way 06-27-16 Set Up Trap Maywood Circle, Berlin Street 06-28-16 Administrative Contact Police Department Public Relations Perry Place, Highland Street, Rockland Road, Rockland Road Court, Idlewood Drive, Colonial, Road, Shore Drive, TOWN OF AUBURN

DATE WORK DONE LOCATION

Alpine Terrace, Pineview Terrace, Chestnut Avenue, Hanna Drive, Nancy Drive, Auburn Street, Renaud Drive, Main Street, Prospect Parkway, Oak Street, Park Street, Putnam Lane, Eastford Road, Homestead Avenue, Swanson Road, Faneuff Street, Jeffrey Avenue, Hawthorne Street, Pinrock Road, Southwest Cutoff, Windbrook Drive, Kellogg Street, Bancroft Street, Robertson Road, Overhill Park, Goddard Drive, Curtis Street, Stonebridge Drive, Saybrook Way, Thayer Avenue, Davis Road, Summer Hill Road, Lesley Avenue, Manor Road, Upland Street, Meadowbrook Road, Old Farm Road, Rydberg Terrace Extension, Bryn Mawr Avenue, Regis Drive, Mayfield Road, Old Cart Road, Inwood Road, Bylund Avenue, Wallace Avenue, Pine Brook Court, Carroll Street, Pinehurst Avenue, Marilyn Drive, Mohawk Avenue, Montello Street, Burnett Street, Silver Street, Leicester Street, Rochdale Street Adulticiding Perry Place, Highland Street, Rockland Road, Rockland Road Court, Idlewood Drive, Colonial, Road, Shore Drive, Alpine Terrace, Pineview Terrace, Chestnut Avenue, Hanna Drive, Nancy Drive, Auburn Street, Renaud Drive, Main Street, Prospect Parkway, Oak Street, Park Street, Putnam Lane, Eastford Road, Homestead Avenue, Swanson Road, Faneuff Street, Jeffrey Avenue, Hawthorne Street, Pinrock Road, Southwest Cutoff, Windbrook Drive, Kellogg Street, Bancroft Street, Robertson Road, Overhill Park, Goddard Drive, Curtis Street, Stonebridge Drive, Saybrook Way, Thayer Avenue, Davis Road, Summer Hill Road, Lesley Avenue, Manor Road, Upland Street, Meadowbrook Road, Old Farm Road, Rydberg Terrace Extension, Bryn Mawr Avenue, Regis Drive, Mayfield Road, Old Cart Road, Inwood Road, Bylund Avenue, Wallace Avenue, Pine Brook Court, Carroll Street, Pinehurst Avenue, Marilyn Drive, Mohawk Avenue, Montello Street, Burnett Street, Silver Street, Leicester Street, Rochdale Street Pick Up Trap Maywood Circle, Berlin Street, Heard Street 06-30-16 Adulticiding Pakachoag Street 07-05-16 Set Up Trap Athens Street 07-06-16 Administrative Contact Police Department Public Relations Hilltop Farm Road, Prospect Street, Bridle Path, Appleton Road, Sherman Avenue, Warren Road, Elbridge Road, Goulding Drive, South Street, Cedar Street, South Street, Coachman Lane, Oxford Street South, Oxford Street, South Terrace, Maybelle Avenue, Diane Avenue, Paul Street, Loring Street, Packard Avenue, Sharon Avenue, Prentice Avenue, Dartmouth Drive, Water Street, Meadow Street, Windsor Avenue, Church Street, Washburn Road, Ridgewood Drive, Saratoga Road, Arrowhead Avenue, Munger Drive, Mt. View Avenue Adulticiding Hilltop Farm Road, Prospect Street, Bridle Path, Appleton Road, Sherman Avenue, Warren Road, Elbridge Road, Goulding Drive, South Street, Cedar Street, South Street, Coachman Lane, Oxford Street South, Oxford Street, South Terrace, Maybelle Avenue, Diane Avenue, Paul Street, Loring Street, Packard Avenue, Sharon Avenue, Prentice Avenue, Dartmouth Drive, Water Street, Meadow Street, Windsor Avenue, Church Street, Washburn Road, Ridgewood Drive, Saratoga Road, Arrowhead Avenue, Munger Drive, Mt. View Avenue Pick Up Trap Athens Street TOWN OF AUBURN

DATE WORK DONE LOCATION

07-08-16 Catch Basin Larviciding Sunrise Avenue, Magna Vista Drive, Elmwood Road, [299] Rochelle Street, Cemetery Road, Havana Road, Lamper Hill Road, Dale Avenue, Burnett Street, Rochdale Street, Westwood Drive, Pinehurst Avenue, Horseshoe Drive, Mohawk Avenue, Courville Road, Marilyn Drive, Old Colony Road, Montello Street, Silver Street, Carroll Street, Holmes Street, Otis Street, Karen Avenue, Gates Court, John William Drive, Duncan Street, Sara Drive, Dixon Avenue, Lorna Drive, Heard Street, Mahlert Court, Robert Avenue, Buttonwood Lane, June Street, Lincoln Street, Chestnut Avenue, Herbert Way, Clark Street, South Edlin Street, Wellman Street, Oxford Street North, White Terrace, Vinal Street, Commonwealth Avenue, Nancy Drive, Oakwood Avenue, Hanna Drive, Rockland Road, Gwen Drive, Idlewood Drive, Alden Street, Maria Avenue, Boyce Street, Colonial Road, Sumner Street, Little Drive, Adella Street, Woodland Road, Shirley Street, Franklin Street 07-11-16 Set Up Trap Tuck Farm Road, Santom Street, Heard Street 07-12-16 Administrative Contact Police Department Public Relations Pinrock Road, Laurel Lane, Montclair Drive, Old Common Road, Stone Street, Elm Street, Camden Drive, School Street, Winchester Avenue, Barbara Avenue, Central Street, Barnes Street, Elizabeth Drive, Gilbert Way, Mt. View Avenue, Reithel Street, Williams Street, South Street, Pakachoag Street, Harrison Avenue, Westlund Avenue, Clarendon Road, Hampton Street, Glenview Road, Exeter Drive, Elmwood Street, Hampton Street, Arlington Street Pick Up Trap Tuck Farm Road, Santom Street, Heard Street Set Up Trap Springbrook Road, Autumn Hill Road, Rydberg Terrace, Washington Street 07-13-16 Pick Up Trap Springbrook Road, Autumn Hill Road, Rydberg Terrace, Washington Street 07-15-16 Catch Basin Larviciding Shore Drive, Newton Street, Forest Drive, Field Street, [300] Alpine Trail, Preston Avenue, Rockaway Road, Jerome Avenue, Sword Street, High Ridge Road, A Street, Gibson Road, B Street, Cooper Street, C Street, Pickering Street, Pineview Trail, Hillside Street, Ravine Drive, Center Street, Bay Ridge Road, Hampton Street, Evers Street, Aurilla Court, Seneca Street, Warwick Street, Elmwood Street, Malvern Road, Willis Street, Clay Street, Mullen Avenue, Lexington Avenue, Eaton Avenue, Ward Street, Vesper Street, Pakachoag Street, Aurilla Street, Newland Street, Erica Lane, Hoover Road, Springbrook Road, Alex Circle, Summer Hill Road, Matnick Circle, Lesley Avenue, Manor Road, Davis Road, Thayer Avenue, Lexington Avenue, Ward Street, Pakachoag Street Trap Site Survey Miller Fence, Holmes Street 07-18-16 Set Up Trap Heard Street (2), Springbrook Road 07-19-16 Administrative Contact Police Department, Board of Health Public Relations Boyce Street, Wallace Avenue, Lorna Drive, Inwood Road, Briarcliff Drive, Park Terrace, Eastford Road, Rockland Road, Shore Road, Highland Street, Old Common Road, South Street, Cedar Street, Barnes Street, Sharon Avenue, Clarendon Road, Meadowbrook Road, Summerhill Road, Warwick Street, Colonial Road, Walsh Avenue Adulticiding Boyce Street, Wallace Avenue, Lorna Drive, Inwood Road, Briarcliff Drive, Park Terrace, Eastford Road, Rockland Road, Shore Road, Highland Street, Old Common Road, South Street, Cedar Street, Barnes Street, Sharon TOWN OF AUBURN

DATE WORK DONE LOCATION

Avenue, Clarendon Road, Meadowbrook Road, Summerhill Road, Warwick Street, Colonial Road, Walsh Avenue Catch Basin Larviciding Pakachoag Street, Pakachoag Crossing, Upland Street, [84] Betty Street, Curtis Street, Stonebridge Road, Southold Road, Saybrook Way, Meadowbrook Road, Auburn Hill Road, Old Farm Road, Amesbury Street, Arlington Street Pick Up Trap Heard Street (2), Springbrook Road 07-25-16 Set Up Trap Heard Street (2), Springbrook Road 07-26-16 Administrative Contact Police Department Public Relations Pinrock Road, Laurel Lane, Old Common Road, Coolidge Street, Winchester Avenue, Camden Drive, Stone Street, South Street, Barnes Street, Gilbert Way, Cedar Street, Oxford Street South, Coachman Lane, Mt. View Avenue, Walsh Avenue, Hilltop Farm Road, Fenwick Circle, Prospect Street, Elbridge Road, Goulding Drive, Oxford Street, Sherwood Road, Berlin Street, Bylund Drive, Wallace Avenue, Dale Avenue, Eastford Road, Bay Ridge Road, Alpine Trail, Warwick Street, Glenview Road, Pakachoag Street Adulticiding Pinrock Road, Laurel Lane, Old Common Road, Coolidge Street, Winchester Avenue, Camden Drive, Stone Street, South Street, Barnes Street, Gilbert Way, Cedar Street, Oxford Street South, Coachman Lane, Mt. View Avenue, Walsh Avenue, Hilltop Farm Road, Fenwick Circle, Prospect Street, Elbridge Road, Goulding Drive, Oxford Street, Sherwood Road, Berlin Street, Bylund Drive, Wallace Avenue, Dale Avenue, Eastford Road, Bay Ridge Road, Alpine Trail, Warwick Street, Glenview Road, Pakachoag Street Pick Up Trap Heard Street (2), Springbrook Road 08-01-16 Set Up Trap Heard Street, Rydberg Terrace, Auburn Hill Road 08-02-16 Administrative Contact Police Department Public Relations Hilltop Farm Road, Appleton Road, Inwood Road, Homestead Avenue, Lorna Drive, Rochdale Street, Pinehurst Avenue, Westlund Avenue, Pakachoag Street, Clarendon Road, Hawthorne Street, Old Common Road, Stone Street, South Street, Gilbert Way, Dartmouth Drive, Sharon Avenue, Winchester Avenue, Mt. View Avenue, Oxford Street South, Jade Hill Road Adulticiding Hilltop Farm Road, Appleton Road, Inwood Road, Homestead Avenue, Lorna Drive, Rochdale Street, Pinehurst Avenue, Westlund Avenue, Pakachoag Street, Clarendon Road, Hawthorne Street, Old Common Road, Stone Street, South Street, Gilbert Way, Dartmouth Drive, Sharon Avenue, Winchester Avenue, Mt. View Avenue, Oxford Street South, Jade Hill Road Catch Basin Larviciding Prospect Street, Sunnyside Road, Hilltop Farm Road, [114] Booth Road, Linden Lane, Fenwick Circle, Kelly Street, Appleton Road, Athens Street, Old Meetinghouse Road, Federal Hill Road, Heritage Drive, Phoenix Road, Shary Lane, Bridle Path, Eddy Street Pick Up Trap Heard Street, Rydberg Terrace, Auburn Hill Road 08-04-16 Trap Site Survey Upland Gardens Drive 08-08-16 Set Up Trap Heard Street, Springbrook Road, Maywood Circle 08-09-16 Administrative Contact Police Department Public Relations Pinrock Road, Laurel Lane, Hawthorne Street, Old Oxford Street North, Regis Drive, Inwood Road, Eastford Road, Burnett Street, Lorna Drive, Rockland Road, Oakwood Avenue, Idlewood Drive, Alpine Terrace, Pineview Terrace, Summer Hill Road, Springbrook Road, Glen View Road, Clarendon Road, Pakachoag Street, Upland Street- TOWN OF AUBURN

DATE WORK DONE LOCATION

Pakachoag Golf Course, Millbury Street, Old Common Road, Elm Street, South Street, Barnes Street, Elizabeth Drive, Gilbert Way, Winchester Avenue, Knowles Street, Winchester Avenue, Loring Street, Sharon Avenue, Mt. View Avenue, Fenwick Circle Adulticiding Pinrock Road, Laurel Lane, Hawthorne Street, Old Oxford Street North, Regis Drive, Inwood Road, Eastford Road, Burnett Street, Lorna Drive, Rockland Road, Oakwood Avenue, Idlewood Drive, Alpine Terrace, Pineview Terrace, Summer Hill Road, Springbrook Road, Glen View Road, Clarendon Road, Pakachoag Street, Upland Street- Pakachoag Golf Course, Millbury Street, Old Common Road, Elm Street, South Street, Barnes Street, Elizabeth Drive, Gilbert Way, Winchester Avenue, Knowles Street, Winchester Avenue, Loring Street, Sharon Avenue, Mt. View Avenue, Fenwick Circle Catch Basin Larviciding Central Street, Dartmouth Drive, Sharon Avenue, Meadow [170] Street, Heritage Lane, Church Street, South Street to Route 20, Winter Street, Knowles Street, Winchester Avenue, Fletcher Road Pick Up Trap Heard Street, Springbrook Road, Maywood Circle 08-11-16 Administrative Contact Police Department Public Relations Newton Street, Field Street, Jerome Avenue, Pickering Street, Arlington Street, Hampton Street, Curtis Street, Meadowbrook Road, Summer Hill Road Adulticiding Malvern Road, Warwick Street, Aurilla Court, Hampton Street, Pickering Street, Aurilla Street, Center Street, Hillside Street, Willis Street, Newton Street, Field Street, Preston Avenue, Elmwood Street, High Ridge Road, Gibson Road, Amesbury Street, Arlington Street, Mullen Avenue, Glenview Road, Exeter Drive, Highland Drive, Hilton Avenue, Cutting Avenue, Wyoma Drive, Kimball Road, Clarendon Road, Jerome Avenue, Pakachoag Street, Alex Circle, Erica Lane, Matnick Circle, Hoover Road, Alex Circle, Summer Hill Road, Springbrook Road, Manor Road, Lesley Avenue, Davis Road, Thayer Road, Lexington Avenue, Ward Street, Pakachoag Street, Upland Street, Old Farm Road, Auburn Hill Road, Meadowbrook Road, Southold Road, Stonebridge Road, Curtis Street, Betty Street, Saybrook Way, Papas Park Catch Basin Larviciding Pakachoag Street, Burnap Lane, Cutting Avenue, Pakachoag [401] Crossing, Goddard Drive, Forest Park Drive, Rice Road, Boyden Lane, Bancroft Street, Robertson Road, Tuck Farm Road, Comrie Road, Maple Drive, Victoria Drive, Lady Slipper Lane, Harrison Avenue, Westlund Avenue, Burnap Street, Rydberg Terrace, Arnold Road, Lower Windbrook Drive, Upper Windbrook Drive, Breezy Road, Pinrock Road, Laurel Lane, Hawthorne Street, Westec Drive, Jade Hill Road, Montclair Drive, Millbury Street, Technology Drive, Mill Street, Commerce Drive, Old Common Road, Pondville Court, May Lane, St. Marie Street, Elm Street, Riverside Drive, Brook Street, Camden Drive, Stone Street, Grove Street, Coolidge Street, George Street, South Street, Carriage Drive, Santom Street, Jacobs Way, Maywood Circle, Cedar Street 08-12-16 Administrative Contact Board of Health Larviciding Elmwood Road, Rockland Court 08-15-16 Set Up Trap Heard Street, Auburn Hill Road (2) 08-16-16 Administrative Contact Police Department, Board of Health Public Relations Oxford Street South, Coachman Lane, Hilltop Farm Road, Prospect Street, Regis Drive, Briarcliff Drive, Dale TOWN OF AUBURN

DATE WORK DONE LOCATION

Avenue, Clarendon Road, Pakachoag Street, Camden Drive, South Street, Loring Street, Sharon Avenue, Windsor Avenue, Arrowhead Avenue, Meadowbrook Road Adulticiding Oxford Street South, Coachman Lane, Hilltop Farm Road, Prospect Street, Regis Drive, Briarcliff Drive, Dale Avenue, Clarendon Road, Pakachoag Street, Camden Drive, South Street, Loring Street, Sharon Avenue, Windsor Avenue, Arrowhead Avenue, Meadowbrook Road Catch Basin Larviciding Barnes Street, Paul Revere Road, Deer Track Road, [83] Gilbert Way, Elizabeth Street, Old Millbury Road, South Street, Oxford Street South, Coachman Lane, Gallant Avenue, Cedar Street Pick Up Trap Heard Street, Auburn Hill Road (2) 08-22-16 Set Up Trap Rydberg Terrace (2) 08-23-16 Administrative Contact Police Department Public Relations Old Common Road, Winchester Avenue, Westchester Drive, Sharon Avenue, Westlund Avenue, Pakachoag Street, Meadowbrook Road, Summerhill Road, Idlewood Drive, Colonial Road, Oakwood Avenue, Williams Street, Rochdale Street, Coachman Lane, Oxford Street South Adulticiding Old Common Road, Winchester Avenue, Westchester Drive, Sharon Avenue, Westlund Avenue, Pakachoag Street, Meadowbrook Road, Summerhill Road, Idlewood Drive, Colonial Road, Oakwood Avenue, Williams Street, Rochdale Street, Coachman Lane, Oxford Street South Pick Up Trap Rydberg Terrace (2) 08-29-16 Set Up Trap Heard Street, Washington Street (2) 08-30-16 Administrative Contact Police Department Public Relations Pinrock Road, Laurel Lane, Hawthorne Street, Old Common Road, Stone Street, South Street, Winchester Avenue, Sharon Avenue, Mt. View Avenue, Pakachoag Street, Clarendon Road, Alpine Terrace, Idlewood Drive, Oakwood Avenue, Rockland Road, Williams Street, Eastford Road, Homestead Avenue, Regis Drive, Oxford Street, Rochdale Street, Bridle Path, Oxford Street South, Coachman Lane, Gilbert Way Adulticiding Pinrock Road, Laurel Lane, Hawthorne Street, Old Common Road, Stone Street, South Street, Winchester Avenue, Sharon Avenue, Mt. View Avenue, Pakachoag Street, Clarendon Road, Alpine Terrace, Idlewood Drive, Oakwood Avenue, Rockland Road, Williams Street, Eastford Road, Homestead Avenue, Regis Drive, Oxford Street, Rochdale Street, Bridle Path, Oxford Street South, Coachman Lane, Gilbert Way Pick Up Trap Washington Street (2) 09-01-16 Administrative Contact Police Department, Board of Health Public Relations Summerhill Road, Pickering Street, Curtis Street Adulticiding Davis Road, Erica Lane, Hoover Road, Lesley Avenue, Lexington Avenue, Manor Road, Matnick Circle, Pakachoag Street, Springbrook Road, Summerhill Road, Thayer Avenue, Plano Street, Ward Street, Cutting Avenue, Curtis Street, Saybrook Way, Stonebridge Road, Meadow Brook Road, Auburn Hill Road, Old Farm Road, Rydberg Terrace, Papas Park 09-06-16 Set Up Trap Heard Street, Springbrook Road (2) 09-07-16 Pick Up Trap Springbrook Road (2) 09-09-16 Catch Basin Larviciding Wallace Avenue, Bylund Avenue, Wallace Terrace, Berlin [131] Street, Pollier Way, Sherwood Road, Ashcroft Street, Inwood Road, Old Cart Road, Mayfield Road, Briarcliff Drive, Wentworth Drive, Regis Drive, Amherst Drive, Bryn Mawr Avenue, Jeffery Avenue, Murray Avenue, Richard TOWN OF AUBURN

DATE WORK DONE LOCATION

Street, Jay Street, Vine Street, Grandview Avenue, Faneuff Street 09-12-16 Pick Up Trap Heard Street Set Up Trap Heard Street, Auburn Hill Road (2), Rochdale Street, Anderson Way 09-13-16 Pick Up Trap Auburn Hill Road (2), Rochdale Street, Anderson Way 09-15-16 Administrative Contact Police Department Public Relations Curtis Street, Summer Hill Road, Arlington Street, Jerome Avenue, Field Street, Hampton Street, Newton Street, Pickering Street Adulticiding Auburn Hill Road, Betty Street, Curtis Street, Davis Road, Erica Lane, Hoover Road, Lesley Avenue, Lexington Avenue, Manor Road, Matnick Circle, Meadowbrook Road, Old Farm Road, Saybrook Way, Springbrook Road, Stonebridge Rod, Summerhill Road, Thayer Avenue, Upland Street, Ward Street, Kimball Road, Clarendon Road, Wyoma Road, Hilton Avenue, Pakachoag Street, Hampton Street, Highlawn Drive, Glenview Road, Arlington Street, Amesbury Street, Elmwood Street, Gibson Road, High Ridge Road, Preston Avenue, Field Street, Newton Street, Willis Street, Pickering Street, Jerome Avenue, Alex Circle, Malvern Road, Aurilla Court, Warwick Street, Exeter Drive, Cooper Street, Hillside Street, Center Street, Newland Street, Mullen Avenue 09-19-16 Administrative Contact Board of Health Set Up Trap Rydberg Terrace (2), Heard Street, Berlin Street, Maywood Circle 09-20-16 Pick Up Trap Rydberg Terrace (2), Heard Street, Berlin Street, Maywood Circle 09-21-16 Source Reduction Eastford Road Tire Removal [1] 09-22-16 Administrative Contact Police Department Public Relations Summerhill Road, Pickering Street Adulticiding Hoover Road, Springbrook Road, Manor Road, Lesley Avenue, Davis Road, Thayer Avenue, Lexington Avenue, Ward Street, Pakachoag Street, Summerhill Road, Rydberg Terrace, Southold Road, Stonebridge Road, Curtis Street, Betty Street, Upland Street, Saybrook Way, Meadowbrook Road, Auburn Hill Road, Old Farm Road, Cutting Avenue, Papas Park 09-26-16 Set Up Trap Auburn Hill Road (2), Crowl Hill Road, Athens Street 09-27-16 Administrative Contact Board of Health Pick Up Trap Auburn Hill Road (2), Crowl Hill Road, Athens Street 09-29-16 Culvert Cleaning (11) Water Street, Central Street, Oxford Street North, Leicester Street, Anderson Way Stream Cleaning 10’ Leicester Street Stream Cleaning 25’ Leicester Street Stream Cleaning 50’ Anderson Way Stream Cleaning 30’ Leicester Street Stream Cleaning 20’ Leicester Street 09-30-16 Pick Up Trap Heard Street 10-21-16 Administrative Contact Board of Health 12-07-16 Stream Cleaning 100’ Water Street Stream Cleaning 25’ Water Street Stream Cleaning 75’ Packard Street Stream Cleaning 40’ Paul Street Stream Cleaning 50’ South Street Stream Cleaning 10’ Cedar Street Stream Cleaning 25’ South Street Stream Cleaning 50’ Barnes Street TOWN OF AUBURN

DATE WORK DONE LOCATION

Stream Cleaning 75’ South Street Stream Cleaning 50’ Barnes Street Stream Cleaning 50’ Barnes Street Stream Cleaning 135’ Elizabeth Street Stream Cleaning 20’ Elizabeth Street Stream Cleaning 50’ Oxford Street South Stream Cleaning 30’ Oxford Street South Stream Cleaning 50’ Oxford Street South Stream Cleaning 15’ Oxford Street South Culvert Cleaning (24) Water Street, Packard Street, Paul Street, South Street, Santom Street, Maywood Circle, Cedar Street, Barnes Street, Old Millbury Road, Elizabeth Street, Oxford Street South 12-16-16 Administrative Contact Board of Health 12-29-16 Stream Cleaning 15’ Oxford Street North Stream Cleaning 50’ Leicester Street Stream Cleaning 25’ Leicester Street Stream Cleaning 50’ Leicester Street Stream Cleaning 110’ Anderson Way Stream Cleaning 115’ Leicester Street Stream Cleaning 50’ Leicester Street Stream Cleaning 65’ Rochdale Street Culvert Cleaning (9) Oxford Street North, Leicester Street, Anderson Way, Rochdale Street

Town of Auburn: Catch Basin Larvicide Program 2016 Ü

WORCESTER

LEICESTER 9/7/2016 §¨¦290 WNV+ 8/2/2016 9/13/2016 WNV+

8/23/2016 WNV+

§¨¦90

UV12

¤£20

MILLBURY

§¨¦395

OXFORD

(Catch Basins Treated: 2070)

CMMCP (2016) Select features of this map courtesy of: SUTTON Office of Geographic Information (MassGIS), Commonwealth of Massachusetts Information Technology Division Catch Basins Surveyed/Treated

2016 SUMMARY

The Central Massachusetts Mosquito Control Project (the Project) currently provides its services to 41 cities and towns throughout Middlesex and Worcester Counties. The Project's headquarters is located at 111 Otis Street, Northboro, MA. Tours of the headquarters or visits to field work sites may be arranged by calling the office in advance. Please call (508) 393-3055 during business hours for more information. The Project practices Integrated Pest Management (IPM), blending state of the art methods and techniques with expertise, experience, and scientific research to provide our member communities with environmentally sound and cost effective mosquito control.

During 2016 the Project received sixteen thousand, two hundred and ninety-four (16,294) requests for service from town residents and officials. Ten thousand (10,000) pounds of Bti (Bacillus thuringiensis israelensis) was applied by helicopter over two thousand (2,000) acres in 3 towns, Chelmsford, Billerica & Boxborough, and two thousand and eight hundred (2,800) pounds by hand over five hundred and sixty (560) acres throughout our service area were applied to area wetlands to reduce the emergence of adult mosquitoes. This represents over two thousand, five hundred and sixty (2,560) acres of wetland that was treated with this mosquito-specific bacterium, significantly reducing adult mosquito populations in these areas. Bti mosquito larvicide is used to treat areas where mosquito larvae are found. We routinely check known larval habitats kept in our database, but also encourage the public to notify us of any areas they suspect could breed mosquitoes. Our field crews will investigate all such requests and treat the area only if surveillance gathered at the time shows an imminent threat of mosquito emergence. Seventy nine thousand, six hundred and eighteen (79,618) catch basins were treated with larvicidal product to control the mosquitoes that seek out these cool dark wet areas to develop, including the Culex mosquito, a major target for West Nile Virus transmission. Two thousand, one hundred and sixteen (2,116) culverts were cleaned in an attempt to eliminate unnecessary standing water and reduce mosquito breeding. This work was done in conjunction with cleaning, clearing, and digging of one hundred and seventy-three thousand, six hundred and ninety- nine (173,699) feet of streams, brooks and ditches. This represents over thirty-two (32) miles of waterways which were cleaned and improved by Project personnel in 2016.

The Mosquito Awareness Program which we offer to elementary schools and other civic organizations in our district has become very popular. Project staff meets with students, teachers or concerned residents to discuss mosquito biology, mosquito habitat, and control procedures. Much of the presentation is directed towards what children and their families can do to prevent mosquitoes from breeding around their homes. Slides, videos, coloring books and other handouts make this an interesting program. This program is tailored to meet the needs of the specific audience. In 2016, CMMCP laboratory personnel and other administrative staff made seventy-eight (78) educational presentations before three thousand one hundred and fifty (3,150) students in twelve (12) Elementary schools. CMMCP gave a presentation on our program to 8 Clarke University students in the Clarke Vector Ecology program and exhibited at four (4) public meetings such as health fairs, Earth Day celebrations and other public events. The administrative staff also presented to three (3) member community town boards at their request, one (1) member community cable access channels, and two (2) annual or special town meetings. 2011 marked the start of the “CMMCP Mosquito Education Program for Seniors” in which presentations are conducted at local senior centers to increase mosquito-borne disease awareness. Three (3) presentations to fifty (50) senior citizens were conducted in 2016 and this program continues to grow. Over 1,000 specialized brochures for this program were distributed through this program; initial funding by a grant received from the Northeastern Mosquito Control Association.

Our goal is to manage all mosquito problems with education, wetlands restoration or larviciding, but we recognize that there are times when adult mosquito spraying is the only viable solution. In such cases specific areas are treated with either hand-held or pickup truck mounted sprayers if surveillance gathered at the time exceeds a pre-determined threshold to warrant an application. This program is offered on a request-only basis, and the exclusion process allows residents and/or town officials to exclude areas under their control from this or any part of our program.

The Project's surveillance program monitors adult mosquito and larval population density, and is the backbone for prescribing various control techniques. Specialized mosquito traps are deployed throughout the Project’s service area to sample for mosquitoes that may be transmitting mosquito-borne diseases. In conjunction with the Mass. Dept. of Public Health we sample in areas suspected of harboring WNV and other viruses. One thousand three hundred and forty-six (1,346) pools (collections) of mosquitoes totaling thirty five thousand, eight hundred and sixty-one (35,861) specimens were tested for mosquito-borne viruses this year. Sixteen (16) collections were identified positive this year; all with West Nile Virus (WNV) – details are available in the Medical Entomology report in this document. CMMCP lab personnel processed one thousand, seven hundred and sixty-nine (1,769) collections of mosquitoes containing seventy seven thousand, eight hundred and seventy (77,870) individual specimens, representing nineteen (19) mosquito species.

Some additional highlights from 2016:

. Resistance management study; no significant resistance to pyrethroids noted, no change recommended in adulticide material choice (see full report).

. Field trials of a naturally-occurring bacterium called spinosad shows promise for pre- hatch spring brood applications, as well as larval cattail mosquito (Cq. perturbans) control.

. Monitoring for the Asian Tiger Mosquito (Ae. albopictus) did not find any specimens of this aggressive, invasive species in the Central Mass. area.

. Resident satisfaction survey: conclusion; overall satisfaction with the adulticide program was 88%, 98% plan to use our services again (see full report).

. CMMCP participates in the EPA’s WasteWise program, tracking our source reduction (tire recycling) efforts. Our efforts in this program were recognized by the EPA – Region 1 in 2014 with an “Environmental Merit Award” for pesticide reduction.

Educational pamphlets are available to anyone interested in learning about mosquito control and the services provided by the Project, and these items are routinely stocked in member Town/City Halls and libraries. Display boards with information on our program are rotated through area Town Halls throughout the year. We also have a website, www.cmmcp.org that has extensive information on mosquito biology, our control procedures, etc. This website has become a model for other Mosquito Projects and has been widely used throughout our service area and beyond.

For Earth Day 2010, CMMCP officially announced a tire recycling program added as a value added service to our member cities and towns. This program operates under grant monies received and the CMMCP operating budget. Tire piles provide suitable areas for larval mosquito development, including those species known to carry West Nile virus. During the course of one season, the potential exists for hundreds or even thousands of mosquitoes to emerge from just one tire. In 2016 we collected a total of four thousand, eight hundred and thirty-one (4,831) tires in thirty (30) member cities and towns, out highest annual total to date – details are available in the Source Reduction/Tire Recycling report in this document. Collections will continue as time and resources allow.

We recently started a pilot program for beaver mitigation in member communities. Several projects including dam breaching and installation of water level control devices were installed in member communities in 2016 – please see the Wetlands Restoration Program report in this document. More information can be found by calling our office at (508) 393- 3055 or on our website at www.cmmcp.org/beaver_program.htm.

We would like to thank you for your support during 2016 and we look forward to helping you and your community with its mosquito problems in 2017 and beyond.

Respectfully submitted,

Timothy D. Deschamps, Executive Director COMMONWEALTH OF MASSACHUSETTS STATE RECLAMATION & MOSQUITO CONTROL BOARD

CENTRAL MASSACHUSETTS MOSQUITO CONTROL PROJECT est. 1973

EXECUTIVE SUMMARY 2016

January 2017 CMMCP MISSION STATEMENT

The objective of the Central Massachusetts Mosquito Control Project (CMMCP) is to attain an efficient, economic mosquito control operation which will provide the best results possible and be consistent with all ecological aspects and the best interests of the member towns.

Our goal is to reduce mosquito exposure to the public, and the potential for disease transmission by mosquitoes, by utilizing proven, sound mosquito control techniques. CMMCP believes the best way to accomplish this task is by practicing an Integrated Pest Management (IPM) approach as it relates to mosquito control in Massachusetts. IPM utilizes a variety of control techniques and evaluation procedures. Control efforts are undertaken only after surveillance data has been collected and analyzed. Training, experience and common sense dictate our response in any given situation.

It is our desire and responsibility for this Project to have the best mosquito control for the communities that we serve.

INTRODUCTION:

The Central Massachusetts Mosquito Control Project currently provides its services to 41 cities and towns throughout Middlesex and Worcester Counties. The Project's headquarters is located at 111 Otis Street, Northboro, MA. Please call (508) 393-3055 during business hours for information. Twenty (20) full time and four (4) summer interns were employed at CMMCP in 2016. This the year we received a total of sixteen thousand, two hundred and ninety-four (16,294) requests for service from town residents and officials. A map of our service area is on page 7.

EDUCATION:

The Mosquito Awareness Program which we offer to elementary schools and other civic organizations in our district has become very popular. Project staff meets with students, teachers or residents to discuss mosquito biology, mosquito habitat, and control procedures. Much of the presentation is directed towards what can be done to prevent mosquitoes from breeding around their homes. This program is tailored to meet the needs of the specific audience. In 2016, CMMCP laboratory personnel and other administrative staff made seventy-eight (78) educational presentations before three thousand one hundred and fifty (3,150) students in twelve (12) Elementary schools. CMMCP gave a presentation on our program to 8 Clarke University students in the Clarke Vector Ecology program and exhibited at four (4) public meetings such as health fairs, Earth Day celebrations and other public events. The administrative staff also presented to three (3) member community town boards at their request, one (1) member community cable access channels, two (2) annual or special town meetings. 2011 marked the start of the “CMMCP Mosquito Education Program for Seniors” in which presentations are conducted at local senior centers to increase mosquito-borne disease awareness. Three (3) presentations to fifty (50) senior citizens were conducted in 2016 and this program

2 continues to grow. Over 1,000 specialized brochures for this program were distributed through this program; initial funding by a grant received from the Northeastern Mosquito Control Association. Several different educational pamphlets are available to anyone interested in learning about mosquito control and the services provided by the Project, and these items are routinely stocked in member Town/City Halls and libraries. Display boards with information on our program are rotated in area Town/City Halls throughout the year. Bookmarks with educational information have been printed and stocked in member libraries and town halls, and are used as part of the education program. We also have a website at www.cmmcp.org that has extensive information on mosquito biology, our control procedures, products we use, etc.

DITCH MAINTENANCE & WETLAND RESTORATION:

As part of our effort to reduce the need for pesticides we continue to place great emphasis our wetlands restoration program. By cleaning clogged, degraded and overgrown waterways, mosquito breeding from that area can be reduced or eliminated and drainage areas are restored to historic conditions. Two thousand, one hundred and sixteen (2,116) culverts were cleaned in an attempt to eliminate unnecessary standing water and reduce mosquito breeding. This work was done in conjunction with cleaning, clearing, and digging of one hundred and seventy-three thousand, six hundred and ninety-nine (173,699) feet of streams, brooks and ditches. This represents over thirty-two (32) miles of waterways which were cleaned and improved by Project personnel in 2016.

ARBOVIRUS CONTROL:

As part of our West Nile Virus (WNV) prevention program, seventy nine thousand, six hundred and eighteen (79,618) catch basins were treated with larvicidal products to control the mosquitoes that seek out these cool dark wet areas to develop, including the Culex species of mosquito, a major target for West Nile Virus transmission. We identify priority areas in each town and treat the basins in these selected areas to reduce the emergence of this arbovirus. The priority areas are as follows: Prior year WNV activity; senior centers & over 55 housing developments; recreation areas; schools and neighborhoods (higher density first); industrial areas. We performed pre-emptive treatments in late May in areas that showed West Nile Virus in the prior year, with follow up treatments later in the season as part of our standard protocol treatment.

MOSQUITO SURVEILLANCE:

The Project's surveillance program monitors adult mosquito and larval population density, and is the backbone for prescribing various control techniques. Specialized mosquito traps are deployed throughout the Project’s service area to sample for mosquitoes that may be transmitting mosquito-borne diseases. In conjunction with the Mass. Dept. of Public Health we sample in areas suspected of harboring WNV and other viruses. One thousand three hundred and forty-six (1,346) pools (collections) of mosquitoes totaling thirty five thousand, eight hundred and sixty-one (35,861) specimens were tested for mosquito-borne viruses this year. Sixteen (16) collections were identified positive this

3 year; all with West Nile Virus (WNV). CMMCP lab personnel processed one thousand, seven hundred and sixty-nine (1,769) collections of mosquitoes containing seventy seven thousand, eight hundred and seventy (77,870) individual specimens, representing nineteen (19) mosquito species.

Target Species Ae. Cq. Cs. Oc. Culex All vexans perturbans melanura canadensis spp. Species No. Pools 75 524 33 103 674 1,769 Total Specimens 1,156 51,025 577 4,826 14,031 77,870 No. Pools WNV + 0 2† 0 0 14† 16† No. Pools EEE + 0 0 0 0 0 0 †Pool of WNV+ Culex pipiens/restuans complex collected in Auburn on 8/2/16 †Pool of WNV+ Coquillettidia perturbans collected in Auburn on 8/2/16 †Pool of WNV+ Coquillettidia perturbans collected in Hopkinton on 8/5/16 †Pool of WNV+ Culex pipiens/restuans complex collected in Chelmsford on 8/16/16 †Pool of WNV+ Culex species collected in Millbury on 8/19/16 †Pool of WNV+ Culex species collected in Auburn on 8/23/16 †Pool of WNV+ Culex species collected in Westborough on 8/24/16 †Pool of WNV+ Culex species collected in Boylston on 8/25/16 †Pool of WNV+ Culex species collected in Millbury on 8/25/16 †Pool of WNV+ Culex species collected in Millbury on 8/25/16 †Pool of WNV+ Culex species collected in Millbury on 9/1/16 †Pool of WNV+ Culex species collected in Auburn on 9/7/16 †Pool of WNV+ Culex species collected in Millbury on 9/8/16 †Pool of WNV+ Culex species collected in Berlin on 9/8/16 †Pool of WNV+ Culex species collected in Auburn on 9/13/16 †Pool of WNV+ Culex species collected in Clinton on 9/15/16

A table with the 2016 arbovirus information for our service area as well as the statewide results is included on page 8. Adult mosquito surveillance began in May and concluded in September. Three (3) full time seasonable employees were hired for the summer to assist our Staff Entomologist in his duties.

LARVAL MOSQUITO CONTROL:

Bti (Bacillus thuringiensis var. israelensis) mosquito larvicide is a species specific, non- reproducing bacterium and is used to treat areas where mosquito larvae are found. Our field crews will investigate areas we have databased and treat the area if surveillance gathered at the time shows an imminent threat of mosquito emergence. Ten thousand (10,000) pounds of Bti (Bacillus thuringiensis israelensis) was applied by helicopter over two thousand (2,000) acres in 3 towns, Chelmsford, Billerica & Boxborough, and two thousand and eight hundred (2,800) pounds by hand over five hundred and sixty (560) acres throughout our service area were applied to area wetlands to reduce the emergence of adult mosquitoes. This represents over two thousand, five hundred and sixty (2,560) acres of wetland that was treated with this mosquito-specific bacterium, significantly reducing adult mosquito populations in these areas. We have several thousand areas catalogued that are checked and treated as needed on a routine basis,

4 and many applications are small, measured in ounces. Larval control began in March and continued throughout the month of September.

ADULT MOSQUITO CONTROL:

Our goal is to manage all mosquito problems with education, wetlands restoration or larviciding, but we recognize that there are times when adult mosquito spraying is the only viable solution. In such cases specific areas are treated with either hand-held or pickup truck mounted sprayers if surveillance gathered at the time exceeds a pre-determined threshold to warrant an application. This program is offered on a request-only basis, and the exclusion process under 333CMR13 allows residents and/or town officials to exclude areas under their control from this or any part of our program. We apply the spray product at the lowest label rate unless mosquito-borne virus has been identified, and then we will consider other application rates depending on weather and other factors. Two hundred and twenty-seven (227) landing counts were performed by Project field staff as additional surveillance or prior to the application of sumithrin to confirm that pre-determined thresholds of mosquitoes were exceeded to warrant an application. Landing rates are suspended when WNV or EEE is identified in Mass. Adult control began in early June and ended in mid-September with the onset of low nighttime temperatures, reduced service requests and low mosquito population density.

RESEARCH AND EFFICACY

While CMMCP is an agency charged with the control of mosquitoes, we strive to check for efficacy of our products and techniques, and whenever possible perform research in new or different areas of mosquito control. Some of our 2016 Research projects were:

 Asian Tiger Mosquito (ATM) Surveillance in Central Mass.  Field Trials of Natular™ G30 for Pre-Hatch Control of Mosquito Larvae in Selected Spring Brood Locations  Field Trials of Natular™ G for Control of Coquillettidia perturbans Larvae in Selected Cattail Locations  Aerial Mosquito Larval Control Program  2016 Resident Survey  Bottle Assays of Field Collected Mosquitoes for Levels of Resistance to Zenivex® E20 in Central Mass

The addition of a fulltime Field Biologist in 2007 allowed these research projects to become more standardized, resulting in increased validity of the findings, reinforced by multiple seasons of trials. We have annual strategy sessions in the fall/winter seasons to plan for field trials and other anticipated research for the upcoming year. CMMCP departments as determined by the Executive Director will be expected to publish annually in such journals as the Journal of the AMCA (JAMCA), the NMCA or NJMCA Proceedings, Wing Beats, and other publications. The Field Biologist composes reports as directed, such as weekly surveillance, rainfall data, aerial larval control, etc. and will graph and track trends as directed. These reports will be disseminated to various parties,

5 i.e. SRMCB, MDPH, CMMCP Commission, posted on the CMMCP website, etc.

SOURCE REDUCTION/TIRE RECYCLING

For Earth Day 2010, CMMCP officially announced a tire recycling program added as a value added service to our member cities and towns. This program operates under grant monies received and the CMMCP operating budget. Tire piles provide suitable areas for larval mosquito development, including those species known to carry West Nile virus. During the course of one season, the potential exists for hundreds or even thousands of mosquitoes to emerge from just one tire. If tires infested with mosquito eggs, larvae or pupae are transported, the potential to introduce mosquito species into new areas and/or the potential for the spread of arboviruses and their transmission may increase significantly.

For these reasons and as a value added service to our member cites and towns, CMMCP has developed a used tire program, consisting of the following guidelines:

 We accept passenger and light truck tires only  The maximum number tires from one property will be 10 at one time, subject to change without notice  Requests for tire removal shall be done according to established procedures  We reserve the right to refuse anything determined to be unsuitable for this program

Tires accepted as part of this program will be sent to an approved facility for recycling or disposal. This program is subject to end without notice. There is no additional cost to residents or municipalities; this program is part of the full suite of mosquito control services offered. In 2016 we collected a total of four thousand, eight hundred and thirty- one (4,831) tires in twenty-three (30) member cities and towns. Collections will continue as time and resources allow.

Some additional highlights from 2016:

 Resistance management study; no significant resistance to pyrethroids noted, no change recommended in adulticide material choice (see full report).  Field trials of a naturally-occurring bacterium called spinosad shows promise for pre- hatch spring brood applications, as well as larval cattail mosquito (Cq. perturbans) control.  Monitoring for the Asian Tiger Mosquito (Ae. albopictus) did not find any specimens of this aggressive, invasive species in the Central Mass. area.  Resident satisfaction survey: conclusion; overall satisfaction with the adulticide program was 88%, 98% plan to use our services again (see full report).  CMMCP participates in the EPA’s WasteWise program, tracking our source reduction (tire recycling) efforts. Our efforts in this program were recognized by the EPA – Region 1 in 2014 with an “Environmental Merit Award” for pesticide reduction.

CMMCP SERVICE AREA – 2016

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Partner, Member, Member, EPA Pesticide Partner, Northeastern New Jersey Environmental EPA WasteWise Mosquito Control Mosquito Control Stewardship Program Program Association Association

Member, Massachusetts Municipal Association Member, MassRecycle

7 2016 SUMMARY TOTALS NOTE: A full report of our work done in 2014 in each community is available on our website in detail at http://www.cmmcp.org/14report.htm.

Service Requests Bti Lbs. Bti Acres Adulticide Adulticide Gallons Acres 16,294 12,800 2,560 352 170,256

Pools Landing Culverts Restoration Catch Basins Tires Sent to MDPH Counts Cleaned Footage Treated Recycled 1,346 51 2,116 173,699 79,618 4,831

ARBOVIRUS SUMMARY 2016

WNV Surveillance Summary – Statewide 2016 Mosquito Pools Positive 189 Animals Positive 0 Humans Positive 15

2016 EEE Surveillance Summary – Statewide Mosquito Pools Positive 4 Animals Positive 0 Humans Positive 0 CMMCP Surveillance Summary 2016 Mosquitoes Collected and Identified 77,870 Mosquito Pools Submitted for testing 1,346 Mosquito Pools Positive WNV 16 Animals Positive WNV 0 Humans Positive WNV 0 Mosquito Pools Positive EEE 0 Animals Positive EEE 0 Humans Positive EEE 0

8 Total Catch Mosquito Total Service Bti Bti Adulticide Adulticide Basins Pools Culverts Restoration Tires Town Requests Pounds Acres Gallons Acres Treated Tested Cleaned Footage Recycled Acton 576 34.0 6.8 12.11 5,692.86 505 31 55 2,802 19 Ashland 372 168.0 33.6 8.79 4,053.10 1,602 26 17 4,185 22 Auburn 900 85.0 17.0 18.61 8,775.71 2,070 47 70 3,474 83 Ayer 91 14.0 2.8 1.50 720.00 398 23 78 2,220 0 Berlin 113 56.0 11.2 3.88 1,981.90 259 31 65 2,875 0 Billerica 921 3063.0 612.6 15.62 7,759.05 1,424 32 21 12,765 188 Blackstone 244 195.0 39.0 5.30 2,498.57 925 28 49 4,925 0 Boxborough 79 4437.0 887.4 1.60 767.62 572 35 40 4,406 6 Boylston 216 71.0 14.2 5.63 2,915.71 467 33 91 2,515 320 Chelmsford 607 2664.0 532.8 13.69 6,235.71 2,519 38 21 10,687 21 Clinton 142 28.0 5.6 3.44 1,630.95 421 37 45 2,715 206 Devens 2 16.0 3.2 0.01 3.33 515 28 27 565 0 Dracut 768 35.0 7.0 14.48 7,988.10 1,662 30 15 4,085 59 Fitchburg 81 25.0 5.0 2.09 1,134.29 6,509 24 64 2,845 345 Gardner 26 42.0 8.4 0.61 314.29 4,531 41 68 2,265 306 Holliston 462 86.5 17.3 11.23 5,308.33 1,072 33 30 2,765 2 Hopedale 181 120.5 24.1 2.81 1,492.86 840 29 18 7,923 0 Hopkinton 561 100.5 20.1 17.21 8,507.62 3,306 27 33 2,965 802 Hudson 228 38.0 7.6 4.75 2,435.24 2,112 36 64 5,305 77 Lancaster 360 12.5 2.5 6.27 2,969.05 354 25 139 2,895 257 Leominster 109 38.0 7.6 2.00 1,041.90 6,634 40 63 3,523 274 Littleton 347 66.0 13.2 5.82 2,991.90 956 29 25 2,605 8 Lowell 136 19.0 3.8 1.09 506.19 10,367 30 17 2,725 42 Lunenburg 451 45.0 9.0 14.57 6,340.95 390 27 88 2,962 762 Marlboro 289 60.0 12.0 5.32 2,781.43 6,680 29 98 2,645 0 Milford 441 133.0 26.6 10.80 5,357.14 2,088 35 25 2,625 75 Millbury 803 136.0 27.2 19.24 9,250.00 1,918 54 48 4,245 186 Millville 206 118.0 23.6 3.55 1,931.90 582 37 99 3,745 0 Natick 652 67.0 13.4 9.73 4,153.33 1,969 26 23 8,926 39 Northboro 323 65.5 13.1 8.73 3,726.67 1,161 34 147 2,340 97 Northbridge 623 94.5 18.9 8.75 4,497.62 1,314 23 28 6,905 5 Sherborn 219 44.0 8.8 8.39 3,881.43 1,233 29 26 2,516 0 Shrewsbury 407 46.5 9.3 7.73 3,300.00 3,326 43 148 3,030 32 Total Catch Mosquito Total Service Bti Bti Adulticide Adulticide Basins Pools Culverts Restoration Tires Town Requests Pounds Acres Gallons Acres Treated Tested Cleaned Footage Recycled Southboro 245 34.5 6.9 6.84 3,134.05 1,341 28 9 6,565 0 Stow 465 25.0 5.0 13.92 6,207.14 609 27 71 6,610 21 Sturbridge 618 126.0 25.2 13.13 5,948.57 833 31 88 3,360 273 Tewksbury 587 82.5 16.5 11.42 5,681.90 1,967 46 9 5,675 211 Webster 384 100.5 20.1 5.02 2,776.19 562 48 21 5,020 18 Westboro 380 85.5 17.1 9.02 3,850.00 287 33 13 5,100 2 Westford 728 85.0 17.0 16.45 9,104.29 1,509 33 40 2,850 71 Wilmington 951 37.0 7.4 21.20 10,609.52 1,829 30 20 4,545 2

Totals 16,294 12,800 2,560 352.35 170,256 79,618 1,346 2,116 173,699 4,831

©2017 ♦ CMMCP Compiled by Timothy D. Deschamps, Executive Director. For reprints please call (508) 393-3055 or e-mail [email protected]. Reports are also posted online at www.cmmcp.org/16report.htm

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