Bull. Natl. Mus. Nat. Sci., Ser. B, 44(1), pp. 41–51, February 22, 2018 Floral Scent Profiles and Flower Visitors in Species of Asarum Series Sakawanum (Aristolochiaceae) Satoshi Kakishima and Yudai Okuyama* Department of Botany, National Museum of Nature and Science, Amakubo 4–1–1, Tsukuba, Ibaraki 305–0005, Japan *E-mail:
[email protected] (Received 18 August 2017; accepted 20 December 2017) Abstract To understand the potential link between the variation in floral scents and pollinators in Asarum, a diverse plant genus of Japan, we conducted analyses of floral volatile compositions as well as field monitoring of flower visitors in species of the genus series Sakawanum. We detected a remarkably large number of floral volatile compounds, and found they are dominated by aliphatics and terpenoids but poor in benzenoids. However, despite a relatively intensive effort, we failed to identify specific species of flower visitors likely contributing well for the cross pollination of these plants. Contradicting to the genetic evidence that these species are generally outcrossing, the visi- tation frequency of the winged insects for their flowers was likely to be low and thus it remained enigmatic how they successfully cross-pollinate in the wild population. Key words: Asarum, floral scent, Heterotropa, pollination, SPME. The Japan archipelago harbors a rich endemic theless, only a few species are examined for the flora and has been designated as one of the biodi- plant-pollinator interactions in the genus (Suga- versity hotspots (Boufford et al., 2005; Mitter- wara, 1988; Mesler and Lu, 1993). The paucity meier et al., 2011). There are 1862 species and of the information on pollination system of Asa- 847 varieties of endemic land plants in Japan, rum in Japan is probably due to several reasons.