bioRxiv preprint doi: https://doi.org/10.1101/2021.04.15.439997; this version posted April 15, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Regulatory phosphorylation site tunes Phosphoglucomutase 1 2 as a metabolic valve to control mobilization of glycogen stores. 3 Sofía Doello 1, Niels Neumann 1, Philipp Spät 2, Boris Maček 2 and Karl Forchhammer 1* 4 1Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 28, 5 72076 Tübingen, Germany 6 2 Department of Quantitative Proteomics, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, 7 Germany 8 *Corresponding author:
[email protected] 9 Classification: Biological Sciences; Microbiology 10 Key words: glycogen metabolism, phosphoglucomutase, glucose-6-phosphate 11 dehydrogenase, phosphorylation, metabolic channeling, carbon flux, oxidative pentose 12 phosphate cycle protein, cyanobacteria 13 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.04.15.439997; this version posted April 15, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 14 Abstract 15 Regulation of glycogen metabolism is of vital importance in organisms of all three 16 kingdoms of life. Although the pathways involved in glycogen synthesis and degradation are 17 well known, many regulatory aspects around the metabolism of this polysaccharide remain 18 undeciphered.