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DOCUMENT RESUME .., ED 159 028 - SE 024 838 AUTHOR Reber, Cornelius I., Ed.. 4 . TITLE Biological Field and Laboratory MethoaS for Measuring . the Quality of Surface 'Waters and Effluents. Program . Element 1BA027. NSTIT Environmental Protection Agency, Cincipnati", Ohio. ,.., REPORT NO EPA-670/4-73-001 . PUBS' DATE Jul 73 ,,. NOTE qv, 196p.; Contains occasions small, light and broken, ." / type; Pas 3-8,of of Weight and M4Wsure missing from docuient prior to being shipped to : EDRS 4r. EDRS PRICE ar-s0.83 HC-$10.03 Plus Poste. DESCRIPTORS Biological S *ences; *Biology; Environmental Technicians; Field. Studies; *Laboratory Techniques; *Sampling; *Statistical Analysis; *Water Pollution . Control; Zoology IDENTIFIERS Environmental Protection Agency ABSTR*T -) This Environmental Protection Agency manual was developed to provide pollution biologigts with the most recent methods for measuring the effects of environmental contaminants on freshwater and marine organisms. The sections of this manual include: (1) Biometrics;(2) Plankton;(3) Periphyton;(4) Macrophyton; (5) Macroinvertebrates; (6) Fish; and'(7) Bioassay. Each of.these sections is divided up into chapters. The Bicmetrics section deals primarily with introductovtstatistics. The section discussing Plankton covers sample col/ection,.preservation, prepartion, analysis and biomass determination. Sample collection, preservation, aialysiS, and preparation for Periphyton and Macrophyton are covered in those respective sections. In the section on Macroinvertebrates, selection of sampling sites, sampling method and data evaluation are described. Also in this section, a, axonomibibliography for the Aicroinvertebrate groups is.A..neIif" . In th Fish section, sample collection, preservation and an is is discussed among other things. In the final section, bioassay techniques for Phytoplankton, Periphyton; Macroinvertebrates, and Fish are described and the Flathead Minnow and Brook Trout Chronic Tests are included. , References are given in all of theabove sections. (MR) a r *******************vit************************************************* )* Reproductions supplied by EDRS are the best thalf can be made * * i fr-Om the original document. * ******************************************************************* U.S. DEPARTMENT OF REALM1. EDUCATION &WELFARE --APA-670/t-73.001 NATIONAL INSTITUTE OF EDUCATION July 1913 THIS,, MENT HAS BEEN REPRO. oute CTLY AS TIECEIVE0 FROM THE r R ORGANIZATION ORIGIN- ATI TS OF VIEW OR OPINIONS STA E T NECESSARILY REPRE: SENT 0 NATIONAL INSTITUTE OF EDUCA POSITION OR POLICY , (.1 BIOLOGIQAL FIELD AND LABORATORY METHODS y)c:; 'FOR 'MEASURING THE QUALITY .OF SURFACE WATERS AND EFFLUENTS Edited-by Cornelius I. Weber, Ph.D. Chief, Biological Methods Analytical Quality Control Laboratory National Environmental Research Center-Cincinnafi Program Element 1BA021 J!, NATIONAL ENVIRONMENTALRESEARC1fCEHTER OFFICE OF RESEARCH AND DEVELOPMENT U.S. ENVIRONMENTALPROTECTION AGENCY C) CINCINNATI, OHIO 45268 e\ Review Notice . This report' has been reviewed by the National Environmental. Research Center, Cincinnati, and, approved for publication. Mention of trade namesor commercial products does riot constitute endorsement or recommendation for use. ,e FOREWORD Man and his environment must be protected from the adverse effects of pesticides, radiation, noise and other forms of pollution, and the uhwise management of solid waste. Efforts to protect the environment re uire a focus that recognizes the interplay bqitween the components of our hysical environmwt air, water, and land. The National Environmentalesearch centers pvide this multidisciplinary focus through programs engaged in studies on the effects of envirdiimental contaminants on man and t bibsphere, and' . da search for w4ys to prevent contamination and to recyclevaluable . resources. This manual was within the National Environmental Research Center Cincinnati to provide pollution biologists with the most ,recOtt 'methods for measuring the effects of environmental contaminants on fresh-, water and marine organisms in field and laboratory studies which arecarried out to establish water qualiW criteria for the recognized beneficial uses of water and to.monitor surface quality. Andrew W. Breidenbach, Ph.D. Director National Environmental Research Center, Cincinnati, Ohio 1 PREFACE This manual was published under Research Objective AchievementPlan IBA -05AEF, "Methods for Determining Biological Parametersof all Wat rs," as part of the National Analytical MethodS.DevelopmenkResearch Pro am. The manual was prepared largely by a standing committeeof senior Agency biologists organized in 1970 to assist.the Biological Methods Branch in the selectmen of methods for, use inroutine field and laboratorwork in fresh and marine waters arising curing short-term enforcement stud es,'swater quality trend monitoring, effluent testing and research projects. The methods contained in this manual are considered by theommittee to be the best available at this time. The manual will be revisean3 new methods will be recommended as the need arises. The, Committee attempted to avoid duplicating field and labOratory methods already adequately described for Agency use in StandardMethods for the Examination Water and Wastewater, 13th edition, and frequent reference is made to this source throughout the manual. Questions and comments regarding the contents of this manual should be directed to: Cornelius I. Weber, Ph.D. Chief, Biological Methods Br rich Analytical Quality Control Laboratory National EnVironmental Research Center U.S. Environmental Protection:Agency, Chicinnati, Ohio 45268 3 V BIOLOGICAL ADVISORY COMMITTEE. January 1, 1973 CHAIRMAN:.Corif(eliusI. Weber,' Ph. D. Name Program Ndme Program Anderson, Max Indiana Office, Region V, Evansville, Maloney, Thomas Natl. Eutropkication Research Pro- IN gram, Co iris, OR Arthur, John W. Natl. Water Quality Lab, Duluth, MN Mathews, John Region VI, Dallas, TX Bugbee, Stephen L. Region VII, Kansas City, MO Murray, Thomas Office of ti & Water Programs, De Ben, Wally Natl?(toastal PollutionResearch Washingto , DC Program, Corvallis, OR Nadeau, Dr. Royal Oil Spill Research Progrart, Edison ), Duffer, Dr. William R. Natl. Water. uality Control Research NJ Program, Ada, OK Nebeker, Dr. Alan V. Western Fish Toxicology Lab, Cor- Gakstattcr, Dr. Jack H. Nat I.Eutrophication Survey Pro- vallis, OR - gram, Corvallis; OR Oldaker, %%Tien Region I, Needham Heights, MA Harkins, Dr. Ralph Region VI, Ada; OK Parrish, Ldys Region VIII, Denveri1C0 Horning,William Newtown Fish Toxicology Lab, New- Phelps, Dr. Donald K. town, OH, -'Natl. Marine Water Quall/y Labora- tory, Narraginett, RI Ischinger, Lee Field Natl. Investigations c'sa Prager, D Cincinnati, OH Natl. Marine Watr Quality Labora- r tory, Narragansett, RI .Jackson, Dr. Herbert W. Natl. Training Center, Cincinnati, OH Pest Karvelis, Ernest Natl.FieldInvestigations Center, Wheeling Office, RegionIII, Cincinnati, OH /". Wheeling, WV SLinsbur, John c Kerr, Pat Natl.Fate of Pollutants Research Region X, Seattle, WA Program, Athens, GA Tebo, Lee Region IV, Athens, GA Keup, Lowell E. Officeof Air & Water Programs, Tljpmas, Nelson A. Large Lakes Research Program, Washington, Df Grosse Jle, MI Kleveno, Conrad Region V, Chicago, IL Tunz r. Milton Region IX, Alameda, CA LaB uy , James Region III, Charlottesville; VA Wagner, Richard A. Region X, Seattle,W A Lassiter, Dr. Ray Region IV, Squtheast Water Lab, Wapher, Richard W. Natl.FieldInvestigati s-tOCen ter, Athens, GA Denver, CO Other personnel who were former members of the Advisory-Committeeor assisted in thereparation of the manual: Austin, R. Ted NatI.Eutrophication Survey, Corvallis, McKim, Dr. James Natl. Water Quality 14, Duluth. MN OR 1 Tckenthun, Kenneth Office of Air and Water Programs, Boyd, Claude E. Savannah River Ecology Lab, Aiken, EPA, Washington, DC SC Mason, William T. Jr. Analytical Quality Control Lab, Collins, DS.-gerY Analytical ,Quality Control Lab, Cincinnati3OH s - Cincinnati, Oil -- 'Lewis, Philip Analytical QualityControl Lab, Garton, Dr. Ronald Western Fish Toxicology Lab, Cor- 72' Cincinnati, OH vallis, OR Schneider, Robert Nol. FieldInvestigationsCenter, Hegre, Di. Stanley Natl.Marine Water QualityLab, Denver, COti arragansett, RI Seeley, Charles Region IX, San Francisco, CA Katko, Albert Na . Eutrophication Survey, Cor- Sinclair, Ralph Natl. Training Cent6"r, Cincinnati, vallis, OR ° OH McFarland, Ben Analytical Quality Control Labora- Stephan, Charles Newtown Fish Tvicology Lab, New- tory, Cincinnati, OH town, OH r / PERSONNEL CONTRIBUTING TO THE BIOLOGICAL METHODS MANUAL r SUBCOMMITTEES: Biometrics Macroinvertebrates Lassiter, Dr! Ray Chairman Tebo, Lee'Chairman/. Harkins, Dr. Ralph Garton, Dr. Ronald Tebo, Lee \\(,. Lewis, Philip A. Mackenthun, Kenneth Plankton Mason, William T., Jr. Maloney, ThomasChairman Nadeau, Dr. Royal Collins, Dr. Gary Phelphs, Dr. Donald De Ben, Wally Sthneider, bert Duffer, Dr. William Sinclair, R ph Katko, Albert Fish Kerr, Pat ' McFarland, Ben LaBuy, JamesChairman Prager; Dr. Jan Karvelis, Ernest Seeley, Gliarles Preston, ROnald Warner, Richard Wagner, Richard' Periphyton-MacrophYton Bioasspy Anderson, Max Chairman Arthur, John .2-- Chairman Boyd, Dr. Claude, E. Hegrej,:Dr. Stanley Bugbee, Stephen L. IsOinger, Lee Keup, Lowell Jackson, Dr. Herbert Kleveno, Conrad ,Maloney, Thomas McKim, Dr. James Nebeker, Dr. Allan Stephan, Charles Thomas, Nelson a4 . .. , .1 INTRODUCTION The role of aquatic biology in the water car, to an actualeld-study in which