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09 SB Tucuman.P65 BIOCELL ISSN 0327 - 9545 2008, 32(2): A33-A101 PRINTED IN ARGENTINA TUCUMAN BIOLOGY ASSOCIATION (Asociación de Biología de Tucumán) Abstracts from the XXIV ANNUAL SCIENTIFIC MEETING November 8 – 10, 2007 Tafí del Valle, Tucumán, Argentina The abstracts have been revised and evaluated by the Scientific Committee of the Tucuman Biology Association A34 ABSTRACTS BIOCELL 32(2), 2008 BIOCELL 32(2), 2008 ABSTRACTS A35 1. 3. DIFFUSION OF Ca (OH)2 PASTES WITH IRRIGATING ORAL LESIONS, SALIVARY AND CREVICULAR FLUID SOLUTIONS IN ROOT DENTIN CHEMICAL PARAMETERS IN HIV/AIDS Bulacio MA, de la Casa ML, López G, Sáez M, Raiden G. Salúm MK1, Recúpero G2, Luna E2, Valdez C2, Costilla Campero Department of Endodontics. School of Dentistry. National Univer- G3, López ME1. sity of Tucuman. Argentina. 1Cát Química Biológica, 2Servicio de Infectología, Hosp Centro de Salud, 3Hosp Padilla, Fac. Odontología, UNT. The purpose of this study was to compare pastes diffusion on den- E-mail: karina.salum@odontología.unt.edu.ar tin using a dye indicator. Eighteen recently extracted human teeth were used. Crowns were Numerous buccal lesion can appear during the progress of AIDS. amputated and a standardized well was prepared on the buccal face. The aim of this work was to relate oral lesions to immunological A step-back technique was used. The pieces were irrigated with a conditions and chemical parameters in total saliva (TS) and gingivo- NaOCl 1% solution and an EDTA 17% solution as final irrigation. crevicular fluid (GCF). One hundred HIV+ patients (Elisa+Western Cement was removed and root canals were filled with Ca (OH)2 Blot) >500 cells/μl (n=52), 200-500 cells/μl (n=27), <200 cells/μl pastes: Ca(OH)2 + physiological solution, Ca(OH)2 + NaOCl 1%, (n=21) were included, with no Antiretroviral Treatment (ARVT) Ca(OH)2 + Chlorhexidine 1%. These vehicles were also used as (n=39) or low ARVT (n=61). Diagnosis of oral lesions (OL) was controls. Apex and access were sealed with glass ionomer cement carried out following A-C Clearing-House (1993). Buccal patholo- and the pieces were placed in Eppendorf tubes containing physi- gies were related to demographic data, immune suppression, ARVT, ological solution. After 21 days, roots were divided into three thirds chemical determinations in TS (Total Proteins (TP), Mucins and and dyed, observed and measured using a magnifying glass. The Peroxidase) and GCF (TP, Hydroxiproline (Hpo), Lactate Dehy- ANOVA test revealed no significant differences in the dyed sur- drogenase (LDH) and Glutamic Oxalacetic Transaminase (GOT). faces in any of the experimental groups. Dentin was not dyed in The Chi-square test was applied. No relationship was found be- the control group. tween OL and sex, age, infection route, instruction level or ARVT, All pastes used showed a similarly colored dentinal surface. The while there was a relation between OL and smoking and between middle third was the least dyed. OL and <200 cells/μl. No relation was found between OL and TP, Calcium Hydroxide-Sodium Hypochlorite–Chlorhexidine. Hpo, LDH or GOT in GCF. Pseudomembranose Candidiasis, peri- Partially supported by CIUNT odontal disease and neoplasia are related to the progression of the disease, while Eritematose Candidiasis was present. High values of TP and muccoproteins in TS would be related to bacterial le- sions and xerostomy to a subclinical disease in the salivary glands. 2. 4. BIOCHEMICAL ANALYSIS OF CREVICULAR FLUID IN USE OF MEDICATIONS FOR PREVALENT ORAL AGGRESSIVE PERIODONTITIS BEFORE AND AFTER DISEASES IN PRIMARY HEALTH CARE CENTERS TREATMENT (PHCC) IN TUCUMAN Castro CE2, Koss MA1, López ME1. Basualdo M, Mitre P, Aybar E, Luna S. 1Cát Química Biológica, 2Cátedra Periodoncia, Facultad de Fac. de Odontología, UNT. E-mail: [email protected] Odontología, UNT. E-mail: cecilia.castro@odontología.unt.edu.ar Caries and periodontal illness have increased in the last decades Inflammation is associated with vascular exudate, which can be due to poor oral hygiene, higher carbohydrate consumption, defi- collected from the gingivo-crevicular fluid (GCF) to analyze the cient nutrition and lack of access to antimicrobial agents. Risk inflammatory and destructive process in biochemical terms. The groups are those with low socioeconomic levels that do not receive objective of this work was to determine the chemical changes in regular dental care or have no access to dental services. The pur- GCF during Aggressive Periodontitis (AP) before and after peri- pose of this work was to determine the supply of medications with odontal treatment. Forty-six patients with diagnosis of AP and 20 respect to dose and time and to evaluate the prescriptive pattern controls (21-35 yrs old) were included in the study. Samples of provided to dentists in different public health care programs. A descriptive cross-sectional study was carried out. A test with closed GCF were collected from 6 places of the buccal cavity 30 d after questions was given to dentists of the primary health car centers the basic therapy was carried out. Periodontal diagnosis included (PHCC) in the eastern and western 6th area of Tucumán, Argentina. plaque and gingival indices, probing depth, insertion level and Results: medication of choice for toothache was ibuprofen, admin- bleeding. Inclusion criteria were: absence of systemic illnesses, istered every 8 h for 3 or 5 days. Paracetamol was used for preg- previous periodontal therapy and no use of antibiotics or anti- nant women. First choice medication for abscesses was ibuprofen inflammatories for the last 6 months. Aspartate Amino transferase plus amoxicillin. As a second choice dexametasone with amoxicillin (AST), Lactate Dehydrogenase (LDH), Alkaline Phosphatase (AP), and ibuprofen was resorted to in some cases and, in others, dipyrone- Collagenase (zimography), hydroxiproline and proteins were deter- metronidazol. Choice of treatment depended not only on the infec- mined. Statistically significant differences (p<0.01) were found for tion but also on the existing medication supply, duration of treat- AST, LDH and AP, proteins and hydroxiproline decreases. ment and dose required. In some cases treatment was changed due Zimography showed lower collagenolytic activity. Biochemical de- to lack of antibiotics with Betalactamase inhibitors or of terminations in GCF could be used as inflammation markers in AP. clorhexidine glyconate 0.12% for local use. Conclusion: more ef- fective drugs such as diclofenac, ketorolac, clorhexidine glyconate 0.12% and systemic antibiotics with IBL should be supplied to CAPS in order to prevent more serious complications. A36 ABSTRACTS BIOCELL 32(2), 2008 5. 7. INMUNOCOMPLEX GLOMERULONEPHRITIS: EXPERI- GENDER DETERMINATION THROUGH DNA AMPLIFI- MENTAL MODEL VALIDATION CATION OF SALIVA AND BLOOD SAMPLES Sosa ML, Gómez J, Abdala M, Alonso J, Andrés J, Fontenla M, Zenteno N, Abramovich M, Orresta M, Palermo A, Ferrari M. Valdez E, Uñates J, Prchal A, Remis J, F. de Petrino S. Cát. de Odontología Legal y Forense. Fac. de Odontología-UNT. Cát. Biología, Facultad de Medicina, UNT. Avda Roca 2100-PB. Av. B. Aráoz 800. Tucumán. Argentina. Tel. (0381–4107319). E- S.M. de Tucumán. E-mail: [email protected] mail: [email protected] We designed an experimental model of inmunocomplex glomeru- Small amounts of saliva samples are found in cigarette butts, enve- lonephritis (GN) and validated it with functional and biochemical lopes, stamps, stained clothes, chewing gum, muzzles, glasses, etc. tests and histopathological and inmunohistochemical analyses. Male In the saliva buccal ephitelium cells are found from which DNA is 3 month old Wistar rats (n=8) were used. GN induction was carried obtained. In sexual chromosomes there numerous genetic markers out by bovine serum albumin inoculation. Proteinuria was detected that can be used for specific purposes. Objectives: to amplify DNA after 4 weeks of GN induction, and 5 weeks after that, animals through blood and saliva samples to determine gender. Materials were sacrificed by anaesthesia overdose and analyses carried out. and methods: blood and saliva samples were collected from men Nephritic animals showed marked disorders in all biochemical de- and women with their previous informed consent. For DNA ex- terminations (proteinuria, creatinine clearance, uremia, proteinemia, traction a commercial matrix was used (Whatman FTA Elute®). lipidic profile). Kidney histology demonstrated glomerular Amplification was performed with a commercial mix (Hot Master hypercellularity with inflammatory infiltrates and glomerular cap- Mix from Eppendorf®) and two primers. Amplification products illary basal membrane thickening. IgG inmunocomplexes were were evaluated in agarose gels 2% with 0.5–2 ug/ml of Etidio Bro- found by inmunofluorescence, correlated with electron dense de- mide. Results: The results of the amplification by PCR of posits in the epithelial basal membrane. Electron microscopy also amelogenine loci showed only one band for females and two bands showed basal membrane thickening and podocyte structural disor- for males. The mix of AMEL F and AMEL R gave amplificons of ders. All these data validate our experimental model. 542 (X) and 368 (Y) pairs of bases visible in agarose gels of 1.5 %- 2%. Conclusion: saliva is a good fluid for DNA obtainment. The primer AMEL F loses specificity associated with other sites in the DNA chain. Spurious products could be removed by modifying hybridation temperature. 6. 8. RELATIONSHIP BETWEEN TSH WITHIN NORMAL ADAPTATION OF RIA DESIGNED
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