Imperial Journal of Interdisciplinary Research (IJIR) Vol-3, Issue-5, 2017 ISSN: 2454-1362, http://www.onlinejournal.in

Some Aquatic Hyphomycetes from District of MP,

Dr. Anamika Dubey

Abstract : During the preliminary investigation of plastic bottles and kept for 24hours to enable the aquatic hyphomycetes from Chhatarpur district of foam to dissolve. Then the foam was preserved by the five species of aquatic adding FAA. Then the foam samples were returned Hyphomycetes encountered in foam samples to the laboratory and observed under research collected from the different streams and rivers. microscope for the presence of conidia of These fungi namely viz.,Flabellospora crassa hyphomycetes. Alasoadura, Flabellospora verticillata Alasoadura, The preparation of permanent slides were done as Flabellospora acuminata Descals & suggested by Volkmann Kohlmeyer and Webster,Helicomyces torquitus Lane and Shearer Kohlmeyer 4. The measurement and and Isthmotricladia gombakiensis Nawawi, are microphotographs of fresh water hypohmycetes being reported for the first time in freshwater were taken. habitats from Madhya Pradesh. Brief notes and Vaucher slides of the fungi reported were deposited illustrations are also provided along with in the Mycology Herbarium, P. G. Dept. of geographicaldistributions of these fungi in India. Bioscience, R. D. V. V. (M.P.) Keywords: Aquatic habitats, hyphomycetes, Identification of the freshwater hyphomycetes were . confirmed with the help of Nilsson, Ingold and Marvonova and other relevant literature5-6. Reports of fungi from India and Madhya Pradesh were confirmed with the help of Kamat et al., Introduction Bhide et al., Mahabale, Bilgrami et al., Sridhar et The innovative work on Freshwater hyphomycetes al., Sarbhoy et al., Jamaluddin et al. and other was started from the work of C.T. Ingold. He relevant literature7-16. named them as 'AquaticHyphomycetes'1. Nilsson, Webster and Descals described themas "Freshwater Result and Discussion Hyphomycetes"2-3. About more than 500 species Flabellospora acuminata Descals and Webster of hyphomycetes are known from freshwater Conidia: acrogenous, main body clavate, apex habitats. Most of the species are described from capitate 4-6 μm diameter, base pedunculate, 5-10 temperate regions and areIngoldian fungi, while μmlong branches 4-6 synchronomous, 40-100x7-15 there is little information on tropical species. In μm, one branch apical, the rest radiating, slightly India the Chhatarpur District of Madhya Pradesh is retrorsely straight, fusiform, apex greatly extended, rich in biodiversity. However, scanty work has cells 3-10. been done on freshwater hyphomycetes in Madhya Habitat: Conidia in foam samples, Ken River, 04 Pradesh. Therefore, the present exploration was Aug. 2013. carried out. Remark: The present fungus is common in occurrence. The measurements and descriptions of conidia are completely agreed with that of Material and Methods Flabellospora acuminata Descals and Webster The foam samples were collected from the different (1982)17. Therefore, it is assigned to that species. streams and rivers of Chhatarpur District of It has been reported for the first time from Madhya Pradesh from both the lentic and the lotic Chhatarpur District of Madhya Pradesh. habitats. Flabellospora crassa Alasoadura Foam analysis: The foam is formed by the Conidia: hyaline with four to five arms, 37- 56 μm movement of the water against natural obstacles long, 3.5- 4 μm wide at the attachment, increase in like stones, twigs and logs,especially in lotic diameter to 5.5- 7.5 μm at the widest part and again systems, constitutes a natural trap for the conidia of narrows gradually towards the tip aquatic hypomycetes. Foam samples were collected which is about 3 μm wide, 3- 5 septa, presence of at morning and evening time. Samples were made very small central stalk. with a scoop and placed in clean wide mouthed

Imperial Journal of Interdisciplinary Research (IJIR) Page 534

Imperial Journal of Interdisciplinary Research (IJIR) Vol-3, Issue-5, 2017 ISSN: 2454-1362, http://www.onlinejournal.in

Habitat: Conidia in foam samples, Ken River, 04 Remark: The present fungus is frequent in Aug. 2013. occurrence (8.4%). The measurements and Distribution in India: Karnataka (Sridhar and descriptions of conidia are completely agreed with Kaveriappa, Himalaya18-20. that of Isthmotricladia gombakiensis (1975)33. It Remark: The present fungus is occasional in has been reported for the first time from Chhatarpur occurrence. The measurements and descriptions of District of Madhya Pradesh. conidia are completely agreed with that of Flabellospora crassa Alasoadura Conclusion assigned to that species. It has been reported for the Frequency occurrence of fungi found is not similar. first time from Chhatarpur District of Madhya Flabellospora acuminata and Flabellospora Pradesh. verticillata common in occurrence, Flabellospora Flabellospora verticillata Alasoadura crassa torquatus are occasional in occurrence, Conidia: multi-radiate, consisting of a main axis gombakiensis Nawawi is frequent in occurrence. and 5 radiating arms. Main axis 14- 30 X 1.5- 2 μm, 2 terminal cell obclavate, each arm 8- 14 septate, 50 μm. Habitat: Conidia in foam samples, Ken River, 04 Aug. 2013. Distribution in India: Distribution in India: (Patil and Kapadnis, 1980) Western Ghats (Subramanian and Bhatt, 1981) Kerala (Sridhar and Kaveriappa, 1985), Karnataka (Sridhar and Kaveriappa, 1984, 1986, 1989; Ramesh and Vijaykumar 2000) 22-28. Remark: The present fungus is common in occurrence. The measurements and descriptions of conidia are completely agreed with that of Flabellospora verticillata Alasoadura it is assigned to that species. It has been reported for the first time from Chhatarpur District of Madhya Pradesh. Helicomyces torquatus Lane & Shearer Conidia: hyaline, dry,solitary, attached eccentricallyand seceding schizolytically, 52-130 μm. in diameter. filament: 4.8 – 7.2 μm. in diameter, multiseptate times,the basal cell bearing a flattened attachment scar. Habitat: Conidia in foam samples, Ken River, 04 Aug.2013. Remark: The measurements and descriptions of conidia are completely agreed with that of Helicomyces t Shearer (1984)30. Therefore, it is assigned to that species. It has been reported for the first time from Chhatarpur District of Madhya Pradesh. Isthmotricladia gombakiensis Nawawi 64: 243- 246,1975 Conidia: hyaline, main axis 20- 27 X 2 mature conidia consisting of four to six arms, the arms are fusiform, 74- 102 μm long, 4- 5.5 μm wide, 9 tapering to 1- 1.5 μm at the apex by very narrow isthmus, 2 μm long, 1- 1.5 μm wide. Habitat: Conidia in foam samples, Ken River, 04 Aug. 2013. Distribution in India: Karnataka (Sridhar and Kaveriappa, 1984 ; Ramesh and Vijaykumar, 2000), Andhra Pradesh (Sarma and Manoharachary, 1989), Maharashtra (Borse and Patil, 2006) 31-32.

Imperial Journal of Interdisciplinary Research (IJIR) Page 535