CONTENTS \ \

OPENING SESSION

F. Ghiretti Homage to Ren§ Lontie

HEMERYTHRINS

D.M. Kurtz Structure, function and oxidation levels of hemerythrin

STRUCTURE AND FUNCTION OF INVERTEBRATE HEMOGLOBINS

S.N. Vinogradov The dissociation of the extracellular hemoglobin of Lumbricus terrestris: A model of its subunit structure

Y. Tsfadia, E. Daniel Subunit structure of earthworm erythrocruorin

F. Shishikura, M.G. Mainwaring, E.C. Yurewicz, J.J. Lightbody, D.A. Walz, S.N. Vinogradov Characterization of trimeric hemoglobin component from the earthworm,Lumbricus terrestris

A. Ghiretti Magaldi, F. Ghiretti, G. Tognon, G. Zanotti The structure of the extracellular hemoglobin of annelids

A. Ghiretti Magaldi, G. Tognon Ophelia bicornis erythrocruorin. Some peculiar features

I. Pilz, M. Theuer, P. Wilhelm, E. Schwarz Structure of annelid hemoglobins - small angle X-ray studies

M. Zagra, V. Mezzasalma, B. Salvato Structural analogies in annelid erythrocruorins

V. Mezzasalma, M. Zagra, L. Di Stefano, B. Salvato Role of SH-groups and S-S bridges in the main subunit (1/12 molecule) of Spirographis spallanzanii chlorocruorin

T. Suzuki, T. Gotoh Subunit structure and amino acid sequence of the extracellular hemoglobin from the polychaete Tylorrhynchus heterochaetus

http://d-nb.info/870031384 VIII

J. Sgouros, T. Kleinschmidt, G. Braunitzer A preliminary study of the hemoglobin of Arenicola marina 73

K. Fushitani, K. Imai, A.F. Riggs Oxygen equilibria of whole molecules, isolated subunits and the subunit trimer of hemoglobin from Lumbricus terrestris 77

L. Moens, M.L. Van Hauwaert, D. Gee1en, G. Verpooten, J. Van Beeumen The amino acid sequence of a structural unit isolated from the high molecular weight globin chains of Artemia sp. 81

E. Chiancone, F. Ascoli, B. Giardina, R. Santucci, P. Vecchini, D. Verzili Quaternary structure and modulation of function in earthworm erythrocruorin 85

Y. Igarashi, K. Kimura, A. Kajita Calcium dependent allosteric modulation and assembly of the giant hemoglobin from the earthworm, Eisenia foetida 89

E. Ilan, I. Hammel, M.M. David, E. Daniel Quaternary structure of erythrocruorin from the aquatic snail Helisoma trivolvis 93

D. Verzili, G. Citro, F. Ascoli, E. Chiancone Immunochemical properties of molluscan hemoglobins 97

C. Spagnuolo, A. Desideri, E. Chiancone, F. Ascoli Studies of the heme environment in molluscan hemoglobins by EPR 101

F. Ascoli, D. Verzili, E. Chiancone The cooperative dimeric hemoglobin from the mollusc Scapharca inaequivalvis: structural and functional characterization 107

W.E. Royer, W.E. Love The low resolution structures of the cooperative hemoglobins from the blood clam Scapharca inaequivalvis 111

H. Furuta, A. Kajita Structure and function of the dimeric and tetrameric hemoglobins from the bivalve molki-sc, Anadara broughtonii 117

L. Cordone, L. Di Stefano, G.C. Russo Cosolvent effects on hemoproteins1 oxygen uptake and equilibria 121

R.C. Terwilliger, N.B. Terwilliger, E. Schabtach Hemoglobin from the parasitic barnacle, Briarosaccus callosus 125

A.J. Arp Sulfide-binding by an extracellular hemoglobin 129 IX

STRUCTURE OF ARTHROPODAN HEMOCYANINS 133

A. Volbeda, W.G.J. Hoi Three-dimensional structure of haemocyanins from the spiny lobster Panulirus interruptus, at 3.2 A resolution 135

H.J. Bak, N.M. Soeter, J.M. Vereijken, P.A. Jekel, B. Neuteboom, J.J. Be interna Primary structure of the a chain of Panulirus interruptus hemocyanin 149

N.M. Soeter, J.J. Beintema, P.A. Jekel, H.J. Bak, J.M. Vereijken, B. Neuteboom Subunits a, b and c of Panulirus interruptus hemocyanin and evolution of arthropod hemocyanins 153

N.M. Soeter, P.A. Jekel, J.J. Beintema Primary structure of subunit b of Panulirus interruptus hemocyanin 165

B. Neuteboom, G.J.J. Beukeveld, J.J. Beintema Amino acid sequence studies on the £ chain of Panulirus interruptus hemocyanin 169

H.-J. Schneider, W. Voll, L. Lehmann, R. GriBhammer, A. Goettgens, B. Linzen Partial amino acid sequence of crayfish (Astacus leptodactylus) hemocyanin 173

W. Schartau, W. Metzger, P. Sonner, W. Pysny Hemocyanin of the spider Eurypelma californicum: amino acid sequence of subunit a_ and of the smaller CB-peptides of subunits b and c 177

J.N. Lamy, J. Lamy, P. Billiald, P.-Y..Sizaret, J.C. Taveau, N. Boisset, J. Frank, G. Motta Mapping of antigenic determinants in Androctonus austral is hemocyanin: preliminary results 185

P. Reisinger Circular dichroism spectra of native 37 S hemocyanin from the spider Eurypelma californicum and its subunits 203

G.B. Treacy, P.D. Jeffrey Subunit heterogeneity and aggregate formation in Cherax destructor and Jasus sp. hemocyanins 207

W. Stocker, U. Raeder, M.M.C. Bijlholt, W.G. Schutter, T. Wichertjes, J. Markl On the role of individual subunits in the quaternary structure of crustacean hemocyanins 213

W.G. Schutter, W. Keegstra, F. Booy, J. Haker, E.F.J, van Bruggen STEM and cryo-TEM of Limulus and Kelletia hemocyanin 217 SUBUNIT STRUCTURE OF MOLLUSCAN HEMOCYANINS

C. Gielens, C. Benoy, G. Pr§aux, R. Lontie Presence of only seven functional units in the polypeptide chain of the haemocyanin of the cephalopod Octopus vulgaris

T. Wichertjes, C. Gielens, W.G. Schutter, G. Pr§aux R. Lontie, E.F.J, van Bruggen Immuno-EM of Sepia officinalis hemocyanin

J. Lamy, J.N. Lamy, M. Leclerc, S. Compin, K.I. Miller, K.E. van Holde Preliminary results on the structure of Octopus dofleini hemocyanin

F. Richelli, B. Filippi, S. Gobbo, E. Simoni, L. Tallandini, P. Zatta Functional and structural properties of the 50,000 D subunit of Octopus vulgaris hemocyanin

M. Brenowitz, K. Munger, C. Bonaventura, J. Bonaventura Immunochemical relationships and subunit composition of selected molluscan hemocyanins

K.E. van Holde, K.I. Miller Kinetics and equilibria of Octopus hemocyanin association

I. Avissar, E. Daniel, D. Banin, E. Ilan Subunit structure of hemocyanin from the gastropod Levantina hierosolima

EVOLUTION OF HEMOCYANINS

R. Drexel, H.-J. Schneider, S. Sigmund, B. Linzen, C. Gielens, R. Lontie, G. Prfeaux, F. Lottspeich, A. Henschen Partial primary structure of the Helix pomatia Bc-hemocyariin functional unit d

T. Takagi Amino acid sequence of the C-terminal domain of octopus (Paroctopus dofleini dofleini) hemocyani

R. Drexel, T. Takagi, B. Linzen Sequence homologies of Paroctopus dofleini and Helix pomatia hemocyanin

M. Huber, K. Lerch Active-site and protein structure of tyrosinase: comparison to hemocyanin

C.P. Mangum, G. Godette The hemocyanin of the uniramous arthropods J. Markl, W. Stocker, R. Runzler, E. Precht Immunological correspondences between the hemocyanin -subunits of 86 arthropods: evolution of a multigene protein family

H. Sugita Divergence pattern of horseshoe crabs: immunological constitution and immunological distance of the Asian horseshoe crab's hemocyanins

PHYSIOLOGICAL ROLE AND MODULATION OF HEMOCYANIN FUNCTION

B. McMahon Oxygen binding by hemocyanin: compensation during activity and environmental change

R. Paul Gas exchange and gas transport in the tarantula Eurypelma californicum - an overview

T. Fincke, K. Tiling, R. Paul, B. Linzen Relations between respiration and circulation in the tarantula, Eurypelma californicum

N.B. Terwilliger, R.C. Terwilliger, R. Graham Crab hemocyanin function changes during development

B. Giardina, M. Corda, M.G. Pellegrini, A. Bellelli, A. Cau, S.G. Condo, M. Brunori Sexual and seasonal changes of He from Palinurus eiephas

C.R. Bridges, S. Morris Modulation of haemocyanin oxygen affinity by L-lactate - a role for other cofactors

S. Morris, C.R. Bridges Novel non-lactate cofactors of haemocyanin oxygen affinity in crustaceans

G. Menestrina, C. Porcelluzzi, R. Antolini The effects of different cations on the electrical properties of the channel formed by Megathura crenulata hemocyanin

F. Pasquali, G. Menestrina, R. Antolini Electrical properties of ionic channels formed by He Iix pomatia hemocyanin in planar lipid bilayers ALLOSTERIC INTERACTION - THEORIES AND EXPERIMENTS

K. Imai, S. Yoshikawa, K. Fushitani, H. Takizawa, T. Handa, H. Kihara Inference of allosteric unit in chlorocruorin, erythrocruorin, and hemerythrin on the basis of the Monod-Wyman-Changeux model

M. Coletta, E. Di Cera, M. Brunori A cooperative model for ligand binding as applied to oxygen carriers

H. Decker, C.H. Robert, S.J. Gill Nesting - an extension of the allosteric model and its application to tarantula hemocyanin

A. Parody-Morreale, C.H. Robert, G.A. Bishop, S.J. Gill Calorimetric analysis of oxygen binding to lobster hemocyanin

H. Decker, B. Richey, S.J. Gill Structural and functional studies on the hemocyanin of the mangrove crab (Scylla serrata)

A. Savel, J. Markl, B. Linzen The spatial range of allosteric interaction in a 24-meric arthropod hemocyanin

J. Markl, A. Savel, B. Knabe, H. Storz, T. Krabbe, S. Abel, B. Markl Mercury ions - a tool to study the specific role of individual subunits in the allosteric interaction of arthropod hemocyanins

R.W. Topham, S. Tesh, C. Bonaventura, J. Bonaventura Active-site heterogeneity as revealed by peroxide and mercury: interactions with purified subunits of Limulus hemocyanin

K.I. Miller, K.E. van Holde Oxygen-linked dissociation and oxygen binding by subunits of Octopus dofleini hemocyanin

L. Zolla, P. Thyberg, R. Rigler, M. Brunori Conformational differences between oxy- and deoxy-hemocyanin

T. Leidescher, B. Linzen Detection of conformational changes in tarantula (Eurypelma californicum) hemocyanin by means of fluorescent probes

M. Beltramini, A. Piazzesi, M. Alviggi, F. Ricchelli, B. Salvato Effects of solvent composition on hemocyanin conformation XIII

THE ACTIVE SITE OF HEMOCYANINS 435

M. Beltramini, A. Piazzesi, M. Alviggi, F. Ricchelli, B. Salvato Half-apo derivatives of hemocyanins 437

B. Salvato, M. Beltramini, A. Piazzesi, M. Alviggi, F. Ricchelli Spectroscopic characterization of a Co(II) derivative of Carcinus maenas hemocyanin 441

R. Witters, D. Van Hoof, W. Deleersnijder, J.-P. Tahon, R. Lontie Preparation of a methaemocyanin of Astacus leptodactylus regenerable with hydroxylamine 445

R. Witters, W. Deleersnijder, J.-P. Tahon, R. Lontie Binuclear electron-paramagnetic-resonance signals of arthropodan methaemocyanins in the presence of nitrite 449

B. Salvato, G.M. Giacometti, M. Alviggi, G. Giacometti EPR observations on the reaction of hemocyanin with azide 453

B. Salvato, G.M. Giacometti, M. Alviggi, G. Giacometti The reaction of hemocyanin from Octopus vulgaris with nitrite. A reinterpretation of the oxidation pathways 457

S.M. Wang, M.N. Chen, J.R. Tzou, N.C. Li Achatina fulica hemocyanin and a dicopper oxygen carrier as model 463

HEMOGLOBINS AND HEMOCYANINS: STUDIES AT THE LEVELS OF DNA AND RNA, AND BIOSYNTHESIS 467

S.M. Jhiang, J.R. Garey, A.F. Riggs Identification of mRNA coding for globin in the chloragogen cells of Lumbricus terrestris 469

A.F. Riggs, C.K. Riggs, R.-J. Lin, H. Domdey Cloning of the cDNA for the globin from the clam, Barbatia reeveana 473

J.R. Garey, A.F. Riggs Identification of a cDNA transcript containing the coding region for F-I globin from Urechis caupo 477

I. Avissar, V. Daniel, E. Daniel A comparative study of arthropod and mollusc hemocyanin mRNA 481 XIV

G. Pr&aux, A. Vandamme, B. de B&thune, M.-P. Jacobs, R. Lontie Haemocyanin-mRNA-rich fractions of cephalopodan decabrachia and of crustacea, their in vivo and in vitro translation 485

B. Kempter Intracellular hemocyanin and site of biosynthesis in the spider Eurypelma californicum 489

R. Voit, H.-J. Schneider Cloning and sequencing of Eurypelma californicum hemocyanin cDNA 495

CONCLUDING REMARKS 4gg

R. Lontie Conclusions 501

AUTHOR INDEX 505

SUBJECT INDEX 509