Polyclonal Antibody to MPP1 (Center) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP52738PU-N Polyclonal Antibody to MPP1 (Center) - Aff - Purified

Alternate names: 55 kDa erythrocyte membrane protein, DXS552E, EMP55, Membrane protein, p55, palmitoylated 1 Quantity: 0.4 ml Concentration: lot specific Background: This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. Uniprot ID: Q00013 NCBI: NP_001159932 GeneID: 4354 Host / Isotype: Rabbit / Ig Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region ( between 304-331aa) of human MPP1. Format: State: Liquid purified Ig fraction Purification: Purified through a Protein A column followed by peptide affinity purification Buffer System: PBS with 0.09% (W/V) Sodium azide Applications: Flow cytometry: 1/10-1/50. Immunohistochemistry on Paraffin sections: 1/50-1/100. Western blot: 1/100-1/500. Enzyme immunoassay: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognizes human MPP1. Add. Information: Molecular Weight: 52296 Da Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: Seo, P.S. et al., Exp. Biol. Med. (Maywood) 234(3): 255-262 (2009) Seo, P.S. et al., Biochim. Biophys. Acta 1793(2): 281-289 (2009) Gosens, I. et al., Hum. Mol. Genet. 16(16): 1993-2003 (2007) Kusunoki, H. et al., Proteins 64(3): 804-807 (2006)

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 1 / 3 AP52738PU-N: Polyclonal Antibody to MPP1 (Center) - Aff - Purified

Funke, L.,et al., Annu. Rev. Biochem. 74: 219-245 (2005) Pictures: Immunohistochemistry analysis in human placenta tissue (Formalin-fixed, Paraffin-embedded) using MPP1 antibody Cat.-No. AP52738PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.

Western blot analysis of MPP1 (arrow) with 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPP1 gene using MPP1 antibody Cat.-No. AP52738PU-N.

Western blot analysis in Jurkat cell line lysates (35ug/lane) using MPP1 antibody Cat.-No. AP52738PU-N. This demonstrates this antibody detected the MPP1 protein (arrow).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 2 / 3 AP52738PU-N: Polyclonal Antibody to MPP1 (Center) - Aff - Purified

flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) using MPP1 antibody Cat.-No. AP52738PU-N, followed by FITC-conjugated goat-anti- rabbit secondary antibodies.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 3 / 3