Using Transfer Learning for Image-Based Cassava Disease
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Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence
viruses Article Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence Keith Saunders 1,* , Jake Richardson 2, David M. Lawson 1 and George P. Lomonossoff 1 1 Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK; [email protected] (D.M.L.); george.lomonossoff@jic.ac.uk (G.P.L.) 2 Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK; [email protected] * Correspondence: [email protected]; Tel.: +44-1603-450733 Received: 10 September 2020; Accepted: 28 October 2020; Published: 30 October 2020 Abstract: Geminivirus particles, consisting of a pair of twinned isometric structures, have one of the most distinctive capsids in the virological world. Until recently, there was little information as to how these structures are generated. To address this, we developed a system to produce capsid structures following the delivery of geminivirus coat protein and replicating circular single-stranded DNA (cssDNA) by the infiltration of gene constructs into plant leaves. The transencapsidation of cssDNA of the Begomovirus genus by coat protein of different geminivirus genera was shown to occur with full-length but not half-length molecules. Double capsid structures, distinct from geminate capsid structures, were also generated in this expression system. By increasing the length of the encapsidated cssDNA, triple geminate capsid structures, consisting of straight, bent and condensed forms were generated. The straight geminate triple structures generated were similar in morphology to those recorded for a potato-infecting virus from Peru. -
Changes to Virus Taxonomy 2004
Arch Virol (2005) 150: 189–198 DOI 10.1007/s00705-004-0429-1 Changes to virus taxonomy 2004 M. A. Mayo (ICTV Secretary) Scottish Crop Research Institute, Invergowrie, Dundee, U.K. Received July 30, 2004; accepted September 25, 2004 Published online November 10, 2004 c Springer-Verlag 2004 This note presents a compilation of recent changes to virus taxonomy decided by voting by the ICTV membership following recommendations from the ICTV Executive Committee. The changes are presented in the Table as decisions promoted by the Subcommittees of the EC and are grouped according to the major hosts of the viruses involved. These new taxa will be presented in more detail in the 8th ICTV Report scheduled to be published near the end of 2004 (Fauquet et al., 2004). Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., and Ball, L.A. (eds) (2004). Virus Taxonomy, VIIIth Report of the ICTV. Elsevier/Academic Press, London, pp. 1258. Recent changes to virus taxonomy Viruses of vertebrates Family Arenaviridae • Designate Cupixi virus as a species in the genus Arenavirus • Designate Bear Canyon virus as a species in the genus Arenavirus • Designate Allpahuayo virus as a species in the genus Arenavirus Family Birnaviridae • Assign Blotched snakehead virus as an unassigned species in family Birnaviridae Family Circoviridae • Create a new genus (Anellovirus) with Torque teno virus as type species Family Coronaviridae • Recognize a new species Severe acute respiratory syndrome coronavirus in the genus Coro- navirus, family Coronaviridae, order Nidovirales -
Multiple Origins of Prokaryotic and Eukaryotic Single-Stranded DNA Viruses from Bacterial and Archaeal Plasmids
ARTICLE https://doi.org/10.1038/s41467-019-11433-0 OPEN Multiple origins of prokaryotic and eukaryotic single-stranded DNA viruses from bacterial and archaeal plasmids Darius Kazlauskas 1, Arvind Varsani 2,3, Eugene V. Koonin 4 & Mart Krupovic 5 Single-stranded (ss) DNA viruses are a major component of the earth virome. In particular, the circular, Rep-encoding ssDNA (CRESS-DNA) viruses show high diversity and abundance 1234567890():,; in various habitats. By combining sequence similarity network and phylogenetic analyses of the replication proteins (Rep) belonging to the HUH endonuclease superfamily, we show that the replication machinery of the CRESS-DNA viruses evolved, on three independent occa- sions, from the Reps of bacterial rolling circle-replicating plasmids. The CRESS-DNA viruses emerged via recombination between such plasmids and cDNA copies of capsid genes of eukaryotic positive-sense RNA viruses. Similarly, the rep genes of prokaryotic DNA viruses appear to have evolved from HUH endonuclease genes of various bacterial and archaeal plasmids. Our findings also suggest that eukaryotic polyomaviruses and papillomaviruses with dsDNA genomes have evolved via parvoviruses from CRESS-DNA viruses. Collectively, our results shed light on the complex evolutionary history of a major class of viruses revealing its polyphyletic origins. 1 Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio av. 7, Vilnius 10257, Lithuania. 2 The Biodesign Center for Fundamental and Applied Microbiomics, School of Life Sciences, Center for Evolution and Medicine, Arizona State University, Tempe, AZ 85287, USA. 3 Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Rondebosch, 7700 Cape Town, South Africa. -
Topics in Viral Immunology Bruce Campell Supervisory Patent Examiner Art Unit 1648 IS THIS METHOD OBVIOUS?
Topics in Viral Immunology Bruce Campell Supervisory Patent Examiner Art Unit 1648 IS THIS METHOD OBVIOUS? Claim: A method of vaccinating against CPV-1 by… Prior art: A method of vaccinating against CPV-2 by [same method as claimed]. 2 HOW ARE VIRUSES CLASSIFIED? Source: Seventh Report of the International Committee on Taxonomy of Viruses (2000) Edited By M.H.V. van Regenmortel, C.M. Fauquet, D.H.L. Bishop, E.B. Carstens, M.K. Estes, S.M. Lemon, J. Maniloff, M.A. Mayo, D. J. McGeoch, C.R. Pringle, R.B. Wickner Virology Division International Union of Microbiological Sciences 3 TAXONOMY - HOW ARE VIRUSES CLASSIFIED? Example: Potyvirus family (Potyviridae) Example: Herpesvirus family (Herpesviridae) 4 Potyviruses Plant viruses Filamentous particles, 650-900 nm + sense, linear ssRNA genome Genome expressed as polyprotein 5 Potyvirus Taxonomy - Traditional Host range Transmission (fungi, aphids, mites, etc.) Symptoms Particle morphology Serology (antibody cross reactivity) 6 Potyviridae Genera Bymovirus – bipartite genome, fungi Rymovirus – monopartite genome, mites Tritimovirus – monopartite genome, mites, wheat Potyvirus – monopartite genome, aphids Ipomovirus – monopartite genome, whiteflies Macluravirus – monopartite genome, aphids, bulbs 7 Potyvirus Taxonomy - Molecular Polyprotein cleavage sites % similarity of coat protein sequences Genomic sequences – many complete genomic sequences, >200 coat protein sequences now available for comparison 8 Coat Protein Sequence Comparison (RNA) 9 Potyviridae Species Bymovirus – 6 species Rymovirus – 4-5 species Tritimovirus – 2 species Potyvirus – 85 – 173 species Ipomovirus – 1-2 species Macluravirus – 2 species 10 Higher Order Virus Taxonomy Nature of genome: RNA or DNA; ds or ss (+/-); linear, circular (supercoiled?) or segmented (number of segments?) Genome size – 11-383 kb Presence of envelope Morphology: spherical, filamentous, isometric, rod, bacilliform, etc. -
Icosahedral Viruses Defined by Their Positively Charged Domains: a Signature for Viral Identity and Capsid Assembly Strategy
Support Information for: Icosahedral viruses defined by their positively charged domains: a signature for viral identity and capsid assembly strategy Rodrigo D. Requião1, Rodolfo L. Carneiro 1, Mariana Hoyer Moreira1, Marcelo Ribeiro- Alves2, Silvana Rossetto3, Fernando L. Palhano*1 and Tatiana Domitrovic*4 1 Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil. 2 Laboratório de Pesquisa Clínica em DST/Aids, Instituto Nacional de Infectologia Evandro Chagas, FIOCRUZ, Rio de Janeiro, RJ, 21040-900, Brazil 3 Programa de Pós-Graduação em Informática, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil. 4 Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil. *Corresponding author: [email protected] or [email protected] MATERIALS AND METHODS Software and Source Identifier Algorithms Calculation of net charge (1) Calculation of R/K ratio This paper https://github.com/mhoyerm/Total_ratio Identify proteins of This paper https://github.com/mhoyerm/Modulate_RK determined net charge and R/K ratio Identify proteins of This paper https://github.com/mhoyerm/Modulate_KR determined net charge and K/R ratio Data sources For all viral proteins, we used UniRef with the advanced search options (uniprot:(proteome:(taxonomy:"Viruses [10239]") reviewed:yes) AND identity:1.0). For viral capsid proteins, we used the advanced search options (proteome:(taxonomy:"Viruses [10239]") goa:("viral capsid [19028]") AND reviewed:yes) followed by a manual selection of major capsid proteins. Advanced search options for H. -
Diversity of Large DNA Viruses of Invertebrates ⇑ Trevor Williams A, Max Bergoin B, Monique M
Journal of Invertebrate Pathology 147 (2017) 4–22 Contents lists available at ScienceDirect Journal of Invertebrate Pathology journal homepage: www.elsevier.com/locate/jip Diversity of large DNA viruses of invertebrates ⇑ Trevor Williams a, Max Bergoin b, Monique M. van Oers c, a Instituto de Ecología AC, Xalapa, Veracruz 91070, Mexico b Laboratoire de Pathologie Comparée, Faculté des Sciences, Université Montpellier, Place Eugène Bataillon, 34095 Montpellier, France c Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands article info abstract Article history: In this review we provide an overview of the diversity of large DNA viruses known to be pathogenic for Received 22 June 2016 invertebrates. We present their taxonomical classification and describe the evolutionary relationships Revised 3 August 2016 among various groups of invertebrate-infecting viruses. We also indicate the relationships of the Accepted 4 August 2016 invertebrate viruses to viruses infecting mammals or other vertebrates. The shared characteristics of Available online 31 August 2016 the viruses within the various families are described, including the structure of the virus particle, genome properties, and gene expression strategies. Finally, we explain the transmission and mode of infection of Keywords: the most important viruses in these families and indicate, which orders of invertebrates are susceptible to Entomopoxvirus these pathogens. Iridovirus Ó Ascovirus 2016 Elsevier Inc. All rights reserved. Nudivirus Hytrosavirus Filamentous viruses of hymenopterans Mollusk-infecting herpesviruses 1. Introduction in the cytoplasm. This group comprises viruses in the families Poxviridae (subfamily Entomopoxvirinae) and Iridoviridae. The Invertebrate DNA viruses span several virus families, some of viruses in the family Ascoviridae are also discussed as part of which also include members that infect vertebrates, whereas other this group as their replication starts in the nucleus, which families are restricted to invertebrates. -
Clustering and Visualizing the Distribution of Overlapping Reading
bioRxiv preprint doi: https://doi.org/10.1101/2021.06.10.447953; this version posted June 11, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. Clustering and visualizing the distribution of overlapping read- ing frames in virus genomes Laura Munoz-Baena˜ 1 and Art F. Y. Poon1;2 1 Department of Microbiology and Immunology, Western University, London, ON, Canada. 2 Department of Pathology and Laboratory Medicine, Western University, London, ON, Canada. ABSTRACT 1 Gene overlap occurs when two or more genes are encoded by the same nucleotides. This phe- 2 nomenon is found in all taxonomic domains, but is particularly common in viruses, where it may 3 increase the information content of compact genomes or influence the creation of new genes. Here 4 we report a global comparative study of overlapping reading frames (OvRFs) of 12,609 virus refer- 5 ence genomes in the NCBI database. We retrieved metadata associated with all annotated reading 6 frames in each genome record to calculate the number, length, and frameshift of OvRFs. Our re- 7 sults show that while the number of OvRFs increases with genome length, they tend to be shorter 8 in longer genomes. The majority of overlaps involve +2 frameshifts, predominantly found in ds- 9 DNA viruses. However, the longest overlaps involve no shift in reading frame (+0), increasing 10 the selective burden of the same nucleotide positions within codons, instead of exposing additional 11 sites to purifying selection. -
The Influence of Secondary Structure, Selection and Recombination On
Cloete et al. Virology Journal 2014, 11:166 http://www.virologyj.com/content/11/1/166 RESEARCH Open Access The influence of secondary structure, selection and recombination on rubella virus nucleotide substitution rate estimates Leendert J Cloete1†, Emil P Tanov1†, Brejnev M Muhire2, Darren P Martin2 and Gordon W Harkins1*† Abstract Background: Annually, rubella virus (RV) still causes severe congenital defects in around 100 000 children globally. An attempt to eradicate RV is currently underway and analytical tools to monitor the global decline of the last remaining RV lineages will be useful for assessing the effectiveness of this endeavour. RV evolves rapidly enough that much of this information might be inferable from RV genomic sequence data. Methods: Using BEASTv1.8.0, we analysed publically available RV sequence data to estimate genome-wide and gene-specific nucleotide substitution rates to test whether current estimates of RV substitution rates are representative of the entire RV genome. We specifically accounted for possible confounders of nucleotide substitution rate estimates, such as temporally biased sampling, sporadic recombination, and natural selection favouring either increased or decreased genetic diversity (estimated by the PARRIS and FUBAR methods), at nucleotide sites within the genomic secondary structures (predicted by the NASP method). Results: We determine that RV nucleotide substitution rates range from 1.19 × 10-3 substitutions/site/year in the E1 region to 7.52 × 10-4 substitutions/site/year in the P150 region. We find that differences between substitution rate estimates in different RV genome regions are largely attributable to temporal sampling biases such that datasets containing higher proportions of recently sampled sequences, will tend to have inflated estimates of mean substitution rates. -
HTS-Based Diagnostics of Sugarcane Viruses: Seasonal Variation and Its Implications for Accurate Detection
viruses Article HTS-Based Diagnostics of Sugarcane Viruses: Seasonal Variation and Its Implications for Accurate Detection Martha Malapi-Wight 1,*,† , Bishwo Adhikari 1 , Jing Zhou 2, Leticia Hendrickson 1, Clarissa J. Maroon-Lango 3, Clint McFarland 4, Joseph A. Foster 1 and Oscar P. Hurtado-Gonzales 1,* 1 USDA-APHIS Plant Germplasm Quarantine Program, Beltsville, MD 20705, USA; [email protected] (B.A.); [email protected] (L.H.); [email protected] (J.A.F.) 2 Department of Agriculture, Agribusiness, Environmental Sciences, Texas A&M University-Kingsville, Kingsville, TX 78363, USA; [email protected] 3 USDA-APHIS-PPQ-Emergency and Domestic Program, Riverdale, MD 20737, USA; [email protected] 4 USDA-APHIS-PPQ-Field Operations, Raleigh, NC 27606, USA; [email protected] * Correspondence: [email protected] (M.M.-W.); [email protected] (O.P.H.-G.) † Current affiliation: USDA-APHIS BRS Biotechnology Risk Analysis Program, Riverdale, MD 20737, USA. Abstract: Rapid global germplasm trade has increased concern about the spread of plant pathogens and pests across borders that could become established, affecting agriculture and environment systems. Viral pathogens are of particular concern due to their difficulty to control once established. A comprehensive diagnostic platform that accurately detects both known and unknown virus species, as well as unreported variants, is playing a pivotal role across plant germplasm quarantine programs. Here we propose the addition of high-throughput sequencing (HTS) from total RNA to the routine Citation: Malapi-Wight, M.; quarantine diagnostic workflow of sugarcane viruses. We evaluated the impact of sequencing depth Adhikari, B.; Zhou, J.; Hendrickson, needed for the HTS-based identification of seven regulated sugarcane RNA/DNA viruses across L.; Maroon-Lango, C.J.; McFarland, two different growing seasons (spring and fall). -
Evidence to Support Safe Return to Clinical Practice by Oral Health Professionals in Canada During the COVID-19 Pandemic: a Repo
Evidence to support safe return to clinical practice by oral health professionals in Canada during the COVID-19 pandemic: A report prepared for the Office of the Chief Dental Officer of Canada. November 2020 update This evidence synthesis was prepared for the Office of the Chief Dental Officer, based on a comprehensive review under contract by the following: Paul Allison, Faculty of Dentistry, McGill University Raphael Freitas de Souza, Faculty of Dentistry, McGill University Lilian Aboud, Faculty of Dentistry, McGill University Martin Morris, Library, McGill University November 30th, 2020 1 Contents Page Introduction 3 Project goal and specific objectives 3 Methods used to identify and include relevant literature 4 Report structure 5 Summary of update report 5 Report results a) Which patients are at greater risk of the consequences of COVID-19 and so 7 consideration should be given to delaying elective in-person oral health care? b) What are the signs and symptoms of COVID-19 that oral health professionals 9 should screen for prior to providing in-person health care? c) What evidence exists to support patient scheduling, waiting and other non- treatment management measures for in-person oral health care? 10 d) What evidence exists to support the use of various forms of personal protective equipment (PPE) while providing in-person oral health care? 13 e) What evidence exists to support the decontamination and re-use of PPE? 15 f) What evidence exists concerning the provision of aerosol-generating 16 procedures (AGP) as part of in-person -
Molecular Analysis of Vanilla Mosaic Virus from the Cook Islands
Molecular Analysis of Vanilla mosaic virus from the Cook Islands Christopher Puli’uvea A thesis submitted to Auckland University of Technology in partial fulfilment of the requirements for the degree of Master of Science (MSc) 2017 School of Science I Abstract Vanilla was first introduced to French Polynesia in 1848 and from 1899-1966 was a major export for French Polynesia who then produced an average of 158 tonnes of cured Vanilla tahitensis beans annually. In 1967, vanilla production declined rapidly to a low of 0.6 tonnes by 1981, which prompted a nation-wide investigation with the aim of restoring vanilla production to its former levels. As a result, a mosaic-inducing virus was discovered infecting V. tahitensis that was distinct from Cymbidium mosaic virus (CyMV) and Odontoglossum ringspot virus (ORSV) but serologically related to dasheen mosaic virus (DsMV). The potyvirus was subsequently named vanilla mosaic virus (VanMV) and was later reported to infect V. tahitensis in the Cook Islands and V. planifolia in Fiji and Vanuatu. Attempts were made to mechanically inoculate VanMV to a number of plants that are susceptible to DsMV, but with no success. Based on a partial sequence analysis, VanMV-FP (French Polynesian isolate) and VanMV-CI (Cook Islands isolate) were later characterised as strains of DsMV exclusively infecting vanilla. Since its discovery, little information is known about how VanMV-CI acquired the ability to exclusively infect vanilla and lose its ability to infect natural hosts of DsMV or vice versa. The aims of this research were to characterise the VanMV genome and attempt to determine the molecular basis for host range specificity of VanMV-CI. -
Strategies for Identifying the Optimal Length of K-Mer in a Viral Phylogenomic Analysis Using Genomic Alignment-Free Method
University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Masters Theses Graduate School 12-2016 Strategies for Identifying the Optimal Length of K-mer in a Viral Phylogenomic Analysis using Genomic Alignment-free Method Qian Zhang University of Tennessee, Knoxville, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_gradthes Recommended Citation Zhang, Qian, "Strategies for Identifying the Optimal Length of K-mer in a Viral Phylogenomic Analysis using Genomic Alignment-free Method. " Master's Thesis, University of Tennessee, 2016. https://trace.tennessee.edu/utk_gradthes/4318 This Thesis is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Masters Theses by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a thesis written by Qian Zhang entitled "Strategies for Identifying the Optimal Length of K-mer in a Viral Phylogenomic Analysis using Genomic Alignment-free Method." I have examined the final electronic copy of this thesis for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Master of Science, with a major in Life Sciences. Dave Ussery, Major Professor We have read this thesis and recommend its acceptance: Mike Leuze, Colleen Jonsson Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) Strategies for Identifying the Optimal Length of K-mer in a Viral Phylogenomic Analysis using Genomic Alignment-free Method A Thesis Presented for the Master of Science Degree The University of Tennessee, Knoxville Qian Zhang December 2016 Copyright © 2016 by Qian Zhang.