RNA-2013-Sasidharan-1394-404.Pdf
Downloaded from rnajournal.cshlp.org on June 4, 2017 - Published by Cold Spring Harbor Laboratory Press Identification of neoblast- and regeneration-specific miRNAs in the planarian Schmidtea mediterranea VIDYANAND SASIDHARAN,1 YI-CHIEN LU,2 DHIRU BANSAL,1 PRANAVI DASARI,1 DEEPAK PODUVAL,1 ASWIN SESHASAYEE,3 ALISSA M. RESCH,4 BRENTON R. GRAVELEY,4,5 and DASARADHI PALAKODETI1,5 1Institute for Stem Cell Biology and Regenerative Medicine, National Center for Biological Sciences, Bangalore 560065, India 2Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York 10065, USA 3National Center for Biological Sciences, Bangalore 560065, India 4Department of Genetics and Developmental Biology, Institute for Systems Genomics, University of Connecticut Stem Cell Institute, University of Connecticut Health Center, Farmington, Connecticut 06030, USA ABSTRACT In recent years, the planarian Schmidtea mediterranea has emerged as a tractable model system to study stem cell biology and regeneration. MicroRNAs are small RNA species that control gene expression by modulating translational repression and mRNA stability and have been implicated in the regulation of various cellular processes. Though recent studies have identified several miRNAs in S. mediterranea, their expression in neoblast subpopulations and during regeneration has not been examined. Here, we identify several miRNAs whose expression is enriched in different neoblast subpopulations and in regenerating tissue at different time points in S. mediterranea. Some of these miRNAs were enriched within 3 h post- amputation and may, therefore, play a role in wound healing and/or neoblast migration. Our results also revealed miRNAs, such as sme-miR-2d-3p and the sme-miR-124 family, whose expression is enriched in the cephalic ganglia, are also expressed in the brain primordium during CNS regeneration.
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