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SY3200 Cell Sorter True Innovation Inside SY3200 Cell Sorter true innovation inside Europe True Innovation The SY3200™ system is one of the most innovative and versatile Flow Cytometry Research platforms available in the market. True innovation drives superior performance and versatility in the SY3200 system. From optics to electronics, revolutionary technology provides solutions that enable the complex applications of today and tomorrow. Advancements in discovery of new markers and new dyes have improved the ability to identify more populations in a single assay. This has advanced the use of flow cytometry in an increasing number of applications as well as challenged instrumentation to continue to adapt to new parameters. The SY3200 has been designed to easily adapt to the ever increasing need for flexibility and sensitivity in new applications. Whereas some systems are merely evolutionary in design, the SY3200 brings revolutionary, innovative and cutting edge technology to elevate the science of flow cytometry. The innovative and flexible laser delivery array allows for ultimate laser compatibility while the unique reflective collection optics ensure maximum fluoresence collection efficiency. Powerful, Sony designed electronics enable collection of high resolution data and ultra high speed cell sorting while maintaining superior sensitivity. Plug and Play PMTs in the Scalable PMT Array (SPA) module provide the ultimate flexibility in collection options while minimizing cost. The SY3200 provides true innovation to deliver a comprehensive cell sorting solution that meets the challenges of the increasing complexity of research flow cytometry applications. Ultimate Configurability The SY3200 platform is designed for configurability to adapt to the demanding needs of a variety of sorting labs. Unique to any system in flow cytometry, the SY3200 is expandable to a dual system layout, effectively enabling two systems in one. All configurations of the SY3200 can share the detector and electronics console to allow for expansion to a second independent system with a marginal increase in lab space. This dual Highly Automated Parallel Sorting (HAPS) configuration of the SY3200 can effectively double your lab’s capacity with a single instrument platform. For example, you can easily half the time of long, rare event sort with a additional sorter setup. Alternatively, one module can be setup for routine immunophenotyping applications, while the second can be dedicated to special applications such as side population assay, basic GFP sorting or optimized for multiple fluorescent protein analysis and sorting. Benchtop System The benchtop system is a compact ergonomic design that fits easily on a standard lab bench. This configuration is highlighted by accessibility to all system components to allow easy configuration of laser and detector setups. Floor Standing System The floor standing system delivers a unique design for labs that are bench space limited. This configuration which includes an integrated workbench can be easily upgraded to a BSC. Integrated Biosafety Cabinet The integrated biosafety cabinet option is yet another truly innovative approach in design that is tailored to the unique requirements of a lab performing biohazardous or sterile sorting. Innovation in Biosafety Since the introduction of the multi-channel Cell Sorting System in 2006, Sony Biotechnology Inc. has been the leader in providing cell sorters with a truly integrated Bio Safety Cabinet. The SY3200 BSC system is no exception, delivering a carefully engineered integrated Bio Safety Cabinet design that provides operator, sample and environmental protection while main- taining an ergonomic and functional workspace. The SY3200 BSC is integrated into a Baker biosafety cabinet thereby exposing only minimum system components inside the hood. The SY3200 BSC provides a compact footprint with internal workspace of 48” width. Even with two HAPs modules installed, there is ample workspace for safe sample handling, pipetting functions and sample preparation. Simple and Safe Biohazard Sorting The Integrated Biosafety configuration has been specially designed to complement the demanding requirements of working in a BSL 2 or 3 laboratory. Only the minimum system components are exposed within the cabinet. This allows for both integrity of a biosafe environment as well as easier maintenance. With the HAPS module or modules elevated above the biosafety cabinet work surface area, the SY3200 guarantees preservation of laminar airflow as well as easier cleaning of all cabinet surfaces. Moreover, with the majority of the system maintained outside the cabinet environment, there is no safety concern when changing optics, lasers, or PMT setup for new applications or service. In addition, the biosafety cabinet model provides continuous The SY3200 BSC system is custom integrated with The Baker aerosol management of the sort chamber and sort collection Company SterilGARD model SG-403A HE, Class II, Type A2 area through the Baker cabinet hepa filter for a simplified Biological Safety Cabinet. The BSC is Certified Class II NSF-49 Bio- biosafety solution. The bench top system also provides an Safety standard. optional aerosol management system for the sort chamber and sort collection area. Remote Control Configurations The SY3200 BSC System also provides as standard the capability for remote multi-user control, especially important in BSL2 or BSL3 labs. The system can be controlled from a remote location for BSL operation with multiple workstations. Remote control capability also enables remote service diagnostics and troubleshooting when necessary to significantly reduce downtime and Many to One service costs. Remote or BSL Operation Innovation in Laser and Light Delivery Quality results can be directly determined by a system’s capability for both laser light management as well as emitted light collection. The SY3200 employs innovative techniques to ensure not only precise delivery of high quality laser light but also to ensure maximum emitted light collection efficiency. Laser Delivery Array — Customizable and Scalable With its open and flexible architecture, the Laser Delivery Array (LDA) enables a broad choice of laser wavelengths and power choices. The SY3200 offers a choice of up to 6 lasers from a selection of 15 wavelengths from leading laser manufacturers. The high quality Omicron LuxX® lasers available for the SY3200 are a small compact design that provide superior power and stability. They are available in the following wavelengths: 375, 405, 425, 445, 457, 488, 642, 685, 785nm. Additional wavelengths are available with the highly reliable Cobalt DPSS lasers: 515, 532, 561 or 594nm. The SY3200 also has an option for the versatile 552nm laser from Oxxius. A high power ultraviolet option is available from Coherent with the Genesis CX STM-Series 355nm. This unique design allows for ideal beam spot dimensions for scatter thereby enabling optimal scatter patterns even at high data rates. The system utilizes an 809nm laser for Forward Scatter and an 830nm laser for Side Scatter. An innovative laser delivery system provides individual beam shapers for each laser path resulting in five focused elliptical beam spots. Software controlled picomotor alignment stages for each laser allows the operator the ability to independently adjust each individual laser path without removing a single instrument cover. Reflective Collection Optics — Superior Sensitivity The SY3200 system features the new multispot Reflective Collection Optic (RCO) design that incorporates an off axis elliptical mirror and a new revolutionary, patented multifaceted free form mirror, that provide unparalleled fluorescence collection efficiency and allow for exceptional laser spatial seperation and isolation and collimation. The RCO ensures exceptional focusing of emitted light onto collection fiber optics for reduced crosstalk and superior sensitivity. Beams spots are spatially separated by either approximately 350 or 950μm from center spot to reduce crosstalk between lasers and different fluorescence collection channels, ultimately minimizing fluorescence compensation and background optical noise. 120 200 150 150 100 150 80 100 100 60 100 Number Number Number Number 40 50 50 50 20 0 0 0 0 -101 101 102 103 104 -101 101 102 103 104 -101 101 102 103 104 -101 101 102 103 104 BV421 FITC PE APC Measurements performed using Sphero™ Rainbow Particles RCP-30-5A Supreme Flexibility Meeting the demand of novel dyes and increasing assay complexity requires maximum system flexibility and scalability. The detector array in the SY3200 has been designed to meet those demands with an innovative plug & play design allowing the system to accommodate any number of combinations of lasers and dyes. Scalable PMT Array — Plug and Play Detectors The SY3200 system incorporates a customizable and Scalable PMT Array (SPA) that provides up to 30 fluorescence detectors per sorting module. In the dual module configuration, the SY3200 can have up to a total of 60 fluorescence detectors. Plug and Play PMTs and filters are easily interchangeable by the operator to allow fast setup for applications without service intervention, eliminating downtime while maximizing flexibility. The SY3200 enables a wide range of fluorescence detection by using High Performance, red sensitive High Gain Hamamatsu HC142-02 type PMTs for all fluorescence detectors. The SPA also houses PMTs for scatter measurements for optimal scatter resolution. Laser Wavelength Application Dye/Fluorochrome Emmaxλ 355 375 405 445 488
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