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Characterisation of an Intron-Split Solanales Microrna
Characterisation of an intron-split Solanales microRNA Zahara Medina Calzada A thesis submitted for the degree of Doctor of Philosophy University of East Anglia School of Biological Sciences September 2017 This copy of the thesis has been supplied on condition that anyone who consults it is understood to recognise that its copyright rests with the author and that use of any information derived there from must be in accordance with current UK Copyright Law. In addition, any quotation or extract must include full attribution “Doing science is very romantic… when it works!” (the friend of a friend of mine) 2 Abstract MicroRNAs (miRNAs) are a distinct class of short endogenous RNAs with central roles in post-transcriptional regulation of gene expression that make them essential for the development and normal physiology of several groups of eukaryotes, including plants. In the last 15 years, hundreds of miRNA species have been identified in plants and great advances have been achieved in the understanding of plant miRNA biogenesis and mode of action. However, many miRNAs, generally those with less conventional features, still remain to be discovered. Likewise, further layers that regulate the pathway from miRNA biogenesis to function and turnover are starting to be revealed. In the present work we have studied the tomato miRNA “top14”, a miRNA with a non-canonical pri-miRNA structure in which an intron is in between miRNA and miRNA*. We have found that this miRNA is conserved within the economically important Solanaceae family and among other members of the Solanales order also agriculturally relevant, like in sweet potato, while its peculiar intron-split pri-miRNA structure is exclusively kept in the more closely related genera Solanum , Capsicum and Nicotiana . -
Genome Skimming for Phylogenomics
Genome skimming for phylogenomics Steven Andrew Dodsworth School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK. Submitted in partial fulfilment of the requirements of the degree of Doctor of Philosophy November 2015 1 Statement of originality I, Steven Andrew Dodsworth, confirm that the research included within this thesis is my own work or that where it has been carried out in collaboration with, or supported by others, that this is duly acknowledged and my contribution indicated. Previously published material is also acknowledged and a full list of publications is given in the Appendix. Details of collaboration and publications are given at the start of each chapter, as appropriate. I attest that I have exercised reasonable care to ensure that the work is original, and does not to the best of my knowledge break any UK law, infringe any third party’s copyright or other Intellectual Property Right, or contain any confidential material. I accept that the College has the right to use plagiarism detection software to check the electronic version of the thesis. I confirm that this thesis has not been previously submitted for the award of a degree by this or any other university. The copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the author. Signature: Date: 16th November 2015 2 Frontispiece: Nicotiana burbidgeae Symon at Dalhousie Springs, South Australia. 2014. Photo: S. Dodsworth. 3 Acknowledgements Firstly, I would like to thank my PhD supervisors, Professor Andrew Leitch and Professor Mark Chase. -
Federal Register/Vol. 82, No. 13/Monday, January 23, 2017/Proposed Rules
8004 Federal Register / Vol. 82, No. 13 / Monday, January 23, 2017 / Proposed Rules DEPARTMENT OF HEALTH AND comment will be made public, you are www.regulations.gov. Submit both HUMAN SERVICES solely responsible for ensuring that your copies to the Division of Dockets comment does not include any Management. If you do not wish your Food and Drug Administration confidential information that you or a name and contact information to be third party may not wish to be posted, made publicly available, you can 21 CFR Part 1132 such as medical information, your or provide this information on the cover [Docket No. FDA–2016–N–2527] anyone else’s Social Security number, or sheet and not in the body of your confidential business information, such comments and you must identify this Tobacco Product Standard for N- as a manufacturing process. Please note information as ‘‘confidential.’’ Any Nitrosonornicotine Level in Finished that if you include your name, contact information marked as ‘‘confidential’’ Smokeless Tobacco Products information, or other information that will not be disclosed except in identifies you in the body of your accordance with 21 CFR 10.20 and other AGENCY: Food and Drug Administration, comments, that information will be applicable disclosure law. For more HHS. posted on http://www.regulations.gov. information about FDA’s posting of • ACTION: Proposed rule. If you want to submit a comment comments to public dockets, see 80 FR with confidential information that you 56469, September 18, 2015, or access SUMMARY: The Food and Drug do not wish to be made available to the the information at: http://www.fda.gov/ Administration (FDA) is proposing a public, submit the comment as a regulatoryinformation/dockets/ tobacco product standard that would written/paper submission and in the default.htm. -
A Number of Pyridine Alkaloids Are Present in Tobacco Leaves
Agric. Biol. Chem., 43 (7), 1421•`1426, 1979 1421 Isolation of 1-(1•L-2•L S-Nornicotino)-1-deoxy- ,3-D-fructofuranose and Its Formation in Flue-curing Process of Tobacco Leaves Akira KOIWAI, Yoichi MIKAMI , Hajime MATSUSHITA and Takuro KISAKI Central Research Institute, the Japan Tobacco and Salt Public Corporation , 6-2, Umegaoka, Midori-ku, Yokohama 227 , Japan Received November 10, 1978 1-(1•L-2•LS-Nornicotino)-1-deoxy-ƒÀ-D-fructofuranose was first isolated from flue-cured leaves of Cherry Red tobacco, Nicotiana tabacum, cv. Bright Yellow. Its structure was established spectrometrically and synthetically. This substance was shown to be formed from nornicotine during flue-curing. Its smoking effect was mild. A number of pyridine alkaloids are present foods and flavor formation.3) Tomita et al.4) in tobacco leaves. In the courses of our have isolated Amadori rearrangement com phytochemical studies on tobacco alkaloids, pounds of several combination products of we have noticed the presence of a fairly large hexose and amino acids from cured tobacco quantity of an isatin-and BrCN-positive alka leaves. However, the complete structures of loid in the flue-cured leaves of Cherry Red these compounds have not been established. strain of Bright Yellow tobacco variety in In this study we isolated an Amadori rear which nornicotine is contained as a principal rangement compound of the combination pro alkaloid. Its presence has also been reported duct of nornicotine and glucose from Cherry for the cured tobacco leaves of DB 101 CR by Red tobacco leaves, physicochemically estab Stephens and Weybrew.1) Nornicotine type lished its structure, and examine its effect on tobacco is generally considered to be light but the smoking flavor. -
The Spatial Distribution of Tobacco Pipe Fragments at the Hudson's Bay Company Fort Vancouver Village Site: Smoking As a Shared and Social Practice
Portland State University PDXScholar Dissertations and Theses Dissertations and Theses Spring 6-20-2013 The Spatial Distribution of Tobacco Pipe Fragments at the Hudson's Bay Company Fort Vancouver Village Site: Smoking as a Shared and Social Practice Katie Ann Wynia Portland State University Follow this and additional works at: https://pdxscholar.library.pdx.edu/open_access_etds Part of the Archaeological Anthropology Commons, and the Social and Cultural Anthropology Commons Let us know how access to this document benefits ou.y Recommended Citation Wynia, Katie Ann, "The Spatial Distribution of Tobacco Pipe Fragments at the Hudson's Bay Company Fort Vancouver Village Site: Smoking as a Shared and Social Practice" (2013). Dissertations and Theses. Paper 1085. https://doi.org/10.15760/etd.1085 This Thesis is brought to you for free and open access. It has been accepted for inclusion in Dissertations and Theses by an authorized administrator of PDXScholar. Please contact us if we can make this document more accessible: [email protected]. The Spatial Distribution of Tobacco Pipe Fragments at the Hudson’s Bay Company Fort Vancouver Village Site: Smoking as a Shared and Social Practice by Katie Ann Wynia A thesis submitted in partial fulfillment of the requirements for the degree of Master of Arts in Anthropology Thesis Committee: Kenneth M. Ames, Chair Douglas C. Wilson Shelby Anderson Portland State University 2013 Abstract This thesis represents one of the first systematic, detailed spatial analyses of artifacts at the mid-19th century Hudson’s Bay Company’s Fort Vancouver Village site, and of clay tobacco pipe fragments in general. -
An Archaeological and Historical Study of the Tobacco Pipe Trade in the Potomac River Valley Ca
University of Tennessee, Knoxville Trace: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 8-2015 Community Formation and the Development of a British-Atlantic Identity in the Chesapeake: An Archaeological and Historical Study of the Tobacco Pipe Trade in the Potomac River Valley ca. 1630-1730 Lauren Kathleen McMillan University of Tennessee - Knoxville, [email protected] This Dissertation is brought to you for free and open access by the Graduate School at Trace: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of Trace: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Lauren Kathleen McMillan entitled "Community Formation and the Development of a British-Atlantic Identity in the Chesapeake: An Archaeological and Historical Study of the Tobacco Pipe Trade in the Potomac River Valley ca. 1630-1730." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, with a major in Anthropology. Barbara J. Heath, Major Professor We have read this dissertation and recommend its acceptance: Gerald F. Schroedl, Elizabeth J. Kellar, Christopher P. Magra Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official student records.) Community Formation and the Development of a British- Atlantic Identity in the Chesapeake: An Archaeological and Historical Study of the Tobacco Pipe Trade in the Potomac River Valley ca. -
Relevant News and Research 7.14 Cessation Assistance: Telephone- and Internet-Based Interventions
Relevant news and research 7.14 Cessation assistance: telephone- and internet-based interventions Last updated July 2021 Research: ................................................................................................................................................. 2 7.14.1 Telephone services (Quitlines) ................................................................................................. 5 7.14.1.1 Efficacy of telephone services............................................................................................. 16 7.14.1.2 The Quitline in Australia ..................................................................................................... 17 7.14.1.3 Telephone services for high-need groups ........................................................................... 17 7.14.2 Text messaging (SMS) services .............................................................................................. 19 7.14.3 Smartphone applications (apps) ............................................................................................ 35 7.14.4 Internet-based interventions ................................................................................................. 51 7.14.4.1 Social media ........................................................................................................................ 67 7.14.5 Increasing smokers’ use of telephone- and internet-based services .................................... 70 News reports: ....................................................................................................................................... -
Roulette Tobacco Self-Medication
Evolution and Human Behavior 35 (2014) 397–407 Contents lists available at ScienceDirect Evolution and Human Behavior journal homepage: www.ehbonline.org Original Article Tobacco use vs. helminths in Congo basin hunter-gatherers: self-medication in humans?☆ Casey J. Roulette a, Hayley Mann b, Brian M. Kemp a, Mark Remiker c, Jennifer W. Roulette a, Barry S. Hewlett a, Mirdad Kazanji d, Sébastien Breurec d, Didier Monchy e, Roger J. Sullivan f, Edward H. Hagen a,⁎ a Department of Anthropology, Washington State University b Department of Anthropology, State University of New York at Binghamton c Oregon Health & Science University d Institut Pasteur Bangui, CAR e Biomnis, Lyon, France f Department of Anthropology, California State University, Sacramento article info abstract Article history: We tested a novel hypothesis that recreational use of neurotoxic plants helps defend against parasites. Initial receipt 6 November 2013 Specifically, we investigated the relationship between smoking and helminthiasis among the Aka, a remote Final revision received 12 May 2014 population of Central African foragers who are avid tobacco smokers, suffer high rates of helminthiasis, and have little-to-no access to commercial anthelmintics. Two hundred and six healthy Aka men provided saliva Keywords: and stool samples. Saliva samples were assayed for cotinine, a nicotine metabolite; a subsample was Evolutionary medicine genotyped for the CYP2A6 enzyme, which metabolizes nicotine. Stool samples were assayed for intestinal Pharmacophagy Substance use helminth -
A Comparative Analysis of Early Late Woodland Smoking Pipes from the Arkona Cluster
Western University Scholarship@Western Electronic Thesis and Dissertation Repository 9-14-2018 1:00 PM Guided by Smoke: A Comparative Analysis of Early Late Woodland Smoking Pipes from the Arkona Cluster Shane McCartney The University of Western Ontario Supervisor Hodgetts, Lisa M. The University of Western Ontario Graduate Program in Anthropology A thesis submitted in partial fulfillment of the equirr ements for the degree in Master of Arts © Shane McCartney 2018 Follow this and additional works at: https://ir.lib.uwo.ca/etd Part of the Archaeological Anthropology Commons Recommended Citation McCartney, Shane, "Guided by Smoke: A Comparative Analysis of Early Late Woodland Smoking Pipes from the Arkona Cluster" (2018). Electronic Thesis and Dissertation Repository. 5757. https://ir.lib.uwo.ca/etd/5757 This Dissertation/Thesis is brought to you for free and open access by Scholarship@Western. It has been accepted for inclusion in Electronic Thesis and Dissertation Repository by an authorized administrator of Scholarship@Western. For more information, please contact [email protected]. Abstract This thesis undertakes a comparative analysis of ceramic and stone pipes recovered from eight archaeological sites located near present day Arkona, Ontario. Commonly known as the Arkona Cluster, these sites date to between A.D. 1000 and 1280 during the early Late Woodland Period and are thought to be part of a borderland region between distinct cultural groups known as the Western Basin Younge Phase and the Early Ontario Iroquoians. Using a combination of distribution and attribute data from each site’s pipe assemblage, I explore how the similarities and differences observed can be used to draw insights into the potential relations between the sites and how they relate to a larger regional context of identity formation, territorialization and intergroup interaction and exchange. -
Statistics Making an Impact
John Pullinger J. R. Statist. Soc. A (2013) 176, Part 4, pp. 819–839 Statistics making an impact John Pullinger House of Commons Library, London, UK [The address of the President, delivered to The Royal Statistical Society on Wednesday, June 26th, 2013] Summary. Statistics provides a special kind of understanding that enables well-informed deci- sions. As citizens and consumers we are faced with an array of choices. Statistics can help us to choose well. Our statistical brains need to be nurtured: we can all learn and practise some simple rules of statistical thinking. To understand how statistics can play a bigger part in our lives today we can draw inspiration from the founders of the Royal Statistical Society. Although in today’s world the information landscape is confused, there is an opportunity for statistics that is there to be seized.This calls for us to celebrate the discipline of statistics, to show confidence in our profession, to use statistics in the public interest and to champion statistical education. The Royal Statistical Society has a vital role to play. Keywords: Chartered Statistician; Citizenship; Economic growth; Evidence; ‘getstats’; Justice; Open data; Public good; The state; Wise choices 1. Introduction Dictionaries trace the source of the word statistics from the Latin ‘status’, the state, to the Italian ‘statista’, one skilled in statecraft, and on to the German ‘Statistik’, the science dealing with data about the condition of a state or community. The Oxford English Dictionary brings ‘statistics’ into English in 1787. Florence Nightingale held that ‘the thoughts and purpose of the Deity are only to be discovered by the statistical study of natural phenomena:::the application of the results of such study [is] the religious duty of man’ (Pearson, 1924). -
1001 Classic Commercials 3 DVDS
1001 classic commercials 3 DVDS. 16 horas de publicidad americana de los años 50, 60 y 70, clasificada por sectores. En total, 1001 spots. A continuación, una relación de los spots que puedes disfrutar: FOOD (191) BEVERAGES (47) 1. Coca-Cola: Arnold Palmer, Willie Mays, etc. (1960s) 2. Coca-Cola: Mary Ann Lynch - Stewardess (1960s) 3. Coca-Cola: 7 cents off – Animated (1960s) 4. Coca-Cola: 7 cents off – Animated (1960s) 5. Coca-Cola: “Everybody Need a Little Sunshine” (1960s) 6. Coca-Cola: Fortunes Jingle (1960s) 7. Coca-Cola: Take 5 – Animated (1960s) 8. Pet Milk: Mother and Child (1960s) 9. 7UP: Wet and Wild (1960s) 10. 7UP: Fresh Up Freddie – Animated (1960s) 11. 7UP: Peter Max-ish (1960s) 12. 7UP: Roller Coaster (1960s) 13. Kool Aid: Bugs Bunny and the Monkees (1967) 14. Kool Aid: Bugs Bunny and Elmer Fudd Winter Sports (1965) 15. Kool Aid: Mom and kids in backyard singing (1950s) 16. Shasta Orange: Frankenstein parody Narrated by Tom Bosley and starring John Feidler (1960s) 17. Shasta Cola: R. Crumb-ish animation – Narrated by Tom Bosley (1960s) 18. Shasta Cherry Cola: Car Crash (1960s) 19. Nestle’s Quick: Jimmy Nelson, Farfel & Danny O’Day (1950s) 20. Tang: Bugs Bunny & Daffy Duck Shooting Gallery (1960s) 21. Gallo Wine: Grenache Rose (1960s) 22. Tea Council: Ed Roberts (1950s) 23. Evaporated Milk: Ed & Helen Prentiss (1950s) 24. Prune Juice: Olan Soule (1960s) 25. Carnation Instant Breakfast: Outer Space (1960s) 26. Carnation Instant Breakfast: “Really Good Days!” (1960s) 27. Carnation: “Annie Oakley” 28. Carnation: Animated on the Farm (1960s) 29. Carnation: Fresh From the Dairy (1960s) 30. -
Johnny Roventini: Call for Philip Morris
Johnny Roventini: Call for Philip Morris http://www.lucyfan.com/johnnyroventini.html An abbreviated version of the following story originally appeared in the Winter 1998/99 issue of our club newsletter. It chronicles Lucille Ball's long association with the Philip Morris company and profiles its "living mascot," Johnny Roventini. While it is not our policy to "editorialize," we admit to having mixed emotions about this topic: we acknowledge that I Love Lucy might never have happened had it not been for Philip Morris' sponsorship of the show, but we also find it hard to forget that both Desi Arnaz (in 1986) and Lucy's second husband, Gary Morton (in 1989), died of lung cancer... Or that Lucy herself died (in 1989) of heart disease. Both ailments have long been linked to smoking. When CBS and Desilu completed the pilot for I Love Lucy in March, 1951, everyone assumed General Foods, the sponsor of Lucille Ball's radio show, would buy it. GF, instead, quickly turned it down -- and the network hawked the project up and down Madison Avenue, hoping to find another sponsor. Finally, advertising mogul Milton Biow bought the show for his client, Philip Morris cigarettes. The tobacco company would sponsor I Love Lucy exclusively from its premiere on October 15, 1951, through Christmas of 1954, then share sponsorship through June of 1955 with Procter and Gamble. From the beginning, Philip Morris made I Love Lucy its own. It commissioned artists in New York to create animated stick figures of Lucy and Desi that would interact with Johnny Roventini, the diminutive bellboy who for nearly two decades had been belting out PM's advertising slogan, "Cal-l-l for-r-r Phil-lip Mor-ray-ssss," Indeed, Johnny's voice and/or face was often the first thing one heard/saw on Monday evening as I Love Lucy opened: 1 of 7 3/9/2013 8:57 AM Johnny Roventini: Call for Philip Morris http://www.lucyfan.com/johnnyroventini.html Like the series itself, Roventini owed his fame to Milton Biow.