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Skeletal and Cardiac Muscle Disorders Caused by Mutations in Genes Encoding Intermediate Filament Proteins

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International Journal of Molecular Sciences Review Skeletal and Cardiac Muscle Disorders Caused by Mutations in Genes Encoding Intermediate Filament Proteins Lorenzo Maggi 1,* , Manolis Mavroidis 2 , Stelios Psarras 2 , Yassemi Capetanaki 2 and Giovanna Lattanzi 3,4,* 1 Neuroimmunology and Neuromuscular Diseases Unit, Fondazione IRCCS Istituto Neurologico Carlo Besta, 20133 Milan, Italy 2 Center of Basic Research, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece; [email protected] (M.M.); [email protected] (S.P.); [email protected] (Y.C.) 3 CNR Institute of Molecular Genetics “Luigi Luca Cavalli-Sforza”, Unit of Bologna, 40136 Bologna, Italy 4 IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy * Correspondence: [email protected] (L.M.); [email protected] (G.L.) Abstract: Intermediate filaments are major components of the cytoskeleton. Desmin and synemin, cytoplasmic intermediate filament proteins and A-type lamins, nuclear intermediate filament proteins, play key roles in skeletal and cardiac muscle. Desmin, encoded by the DES gene (OMIM *125660) and A-type lamins by the LMNA gene (OMIM *150330), have been involved in striated muscle disorders. Diseases include desmin-related myopathy and cardiomyopathy (desminopathy), which can be manifested with dilated, restrictive, hypertrophic, arrhythmogenic, or even left ventricular non-compaction cardiomyopathy, Emery–Dreifuss Muscular Dystrophy (EDMD2 and EDMD3, due to LMNA mutations), LMNA-related congenital Muscular Dystrophy (L-CMD) and LMNA-linked dilated cardiomyopathy with conduction system defects (CMD1A). Recently, mutations in synemin Citation: Maggi, L.; Mavroidis, M.; (SYNM gene, OMIM *606087) have been linked to cardiomyopathy. This review will summarize Psarras, S.; Capetanaki, Y.; clinical and molecular aspects of desmin-, lamin- and synemin-related striated muscle disorders Lattanzi, G. Skeletal and Cardiac Muscle Disorders Caused by with focus on LMNA and DES-associated clinical entities and will suggest pathogenetic hypotheses Mutations in Genes Encoding based on the interplay of desmin and lamin A/C. In healthy muscle, such interplay is responsible Intermediate Filament Proteins. Int. J. for the involvement of this network in mechanosignaling, nuclear positioning and mitochondrial Mol. Sci. 2021, 22, 4256. https:// homeostasis, while in disease it is disturbed, leading to myocyte death and activation of inflammation doi.org/10.3390/ijms22084256 and the associated secretome alterations. Academic Editor: Jose Keywords: desmin; lamin A/C; synemin; desminopathy; muscular laminopathies; cardiomyopathy; Maria Gonzalez-Granado secretome; mechanosignaling; nuclear positioning Received: 3 March 2021 Accepted: 15 April 2021 Published: 20 April 2021 1. Intermediate Filament Proteins The cytoskeleton is formed by three different protein networks: microfilaments, con- Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in stituted by actin, microtubules, which are polymers of tubulins, and intermediate filaments, published maps and institutional affil- which are formed by intermediate filament proteins (IFs). IFs are encoded by over 70 genes iations. differentially expressed in a cell and tissue-specific manner [1]. IFs expression is also influenced by the developmental stage of cells and tissues and by a plethora of disease states [1,2]. Based on similarities in the amino acid sequence and molecular structure, IFs are divided into six subgroups, named type I to type VI [3]. In particular, acidic keratins are type I IFs, basic keratins are type II IFs, desmin, GFAP, perypherin, vimentin and Copyright: © 2021 by the authors. syncoilin are type III IFs, nestin, neurofilament, synemins and internexin are type IV IFs, Licensee MDPI, Basel, Switzerland. This article is an open access article lamins are type V IFs and lens-specific phakinin and filensin are type VI IFs [3,4]. All IFs distributed under the terms and share a common structural organization including a central alpha-helical “rod domain” conditions of the Creative Commons flanked by non-alpha-helical N-terminal (head) and C-terminal (tail) domains. The central Attribution (CC BY) license (https:// alpha-helical domain is formed by three segments separated by two linkers: coil 1A; linker creativecommons.org/licenses/by/ L1; coil 1B; linker L12; and coil 2 [3,4]. This structure assembles into higher order parallel 4.0/). Int. J. Mol. Sci. 2021, 22, 4256. https://doi.org/10.3390/ijms22084256 https://www.mdpi.com/journal/ijms Int. J. Mol. Sci. 2021, 22, x FOR PEER REVIEW 2 of 27 Int. J. Mol. Sci. 2021, 22, 4256 2 of 27 structure assembles into higher order parallel filaments of about 10 nm that contribute to resilience and flexibility in cells subjected to mechanical forces [1,5]. The amino-terminal domainsfilaments of of IFs about are 10necessary nm that for contribute protein asse to resiliencembly, whereas and flexibility the carboxy-terminal in cells subjected domains to me- arechanical involved forces in [organization1,5]. The amino-terminal of the IFs networ domainsk and of functional IFs are necessary protein forinteractions, protein assembly, mostly regulatedwhereas the by carboxy-terminal post-translational domains modifications are involved [4,5]. inIFs organization are located of in the the IFs cytoplasm, network and as keratins,functional desmin, protein vimentin, interactions, GFAP, mostly neurofilaments, regulated by post-translationalinternexin, synemin, modifications peripherin [and4,5]. nestinIFs are that located are components in the cytoplasm, of the ascytoskelet keratins,on, desmin, or in the vimentin, nucleus GFAP,as lamins, neurofilaments, where they forminternexin, the nucleoskeleton synemin, peripherin [3,6]. Lamins, and nestinwhich thatare distinguished are components into of A the and cytoskeleton, B type lamins, or sharein the structural nucleus as features lamins, of where cytoplasmic they form IFs, thebut nucleoskeleton form thinner filaments [3,6]. Lamins, of about which 3.5 nm are anddistinguished hold a nuclear into localization A and B type signal lamins, [1,7,8]. share structural features of cytoplasmic IFs, but form thinner filaments of about 3.5 nm and hold a nuclear localization signal [1,7,8]. 2. Striated Muscle Intermediate Filament Proteins 2. Striated Muscle Intermediate Filament Proteins 2.1. Expression of Muscle Intermediate Filament Proteins 2.1. Expression of Muscle Intermediate Filament Proteins IFs can be ubiquitous or tissue-specific proteins and many of them can be considered IFs can be ubiquitous or tissue-specific proteins and many of them can be considered differentiation markers of a given cell type [9,10]. Several IFs are expressed in striated differentiation markers of a given cell type [9,10]. Several IFs are expressed in striated muscle, some of them being already present in mesenchymal stem cells and myogenic muscle, some of them being already present in mesenchymal stem cells and myogenic precursors, while others appearing upon cellular determination [10]. Nestin, a type VI precursors, while others appearing upon cellular determination [10]. Nestin, a type VI intermediate filament, is found at the very early stages of stem cell differentiation and it intermediate filament, is found at the very early stages of stem cell differentiation and it is maintained in adult skeletal myoblasts, but not in cardiomyocytes [3]. In proliferating is maintained in adult skeletal myoblasts, but not in cardiomyocytes [3]. In proliferating myoblasts, vimentin, type III intermediate filament, is widely expressed. However, in myoblasts, vimentin, type III intermediate filament, is widely expressed. However, in differentiated cardiomyocytes, vimentin is replaced by tissue-specific IFs [3]. Synemin is differentiated cardiomyocytes, vimentin is replaced by tissue-specific IFs [3]. Synemin is a type IV intermediate filament expressed in a high number of cells, including smooth and a type IV intermediate filament expressed in a high number of cells, including smooth striated muscle cells [11,12]. Synemin is also considered a focal adhesion protein due to and striated muscle cells [11,12]. Synemin is also considered a focal adhesion protein due itsto itslocalization localization at atthe the cell cell membranes, membranes, including including muscle muscle costameres, costameres, where where synemin interactsinteracts with with desmin desmin [3]. [3]. Moreover, Moreover, synemin synemin has has been been recently recently implicated implicated in in the the response response toto DNA DNA damage damage as as a a modulator of of non-homologous end-joining end-joining in in a complex including DNA-proteinDNA-protein kinase kinase catalytic catalytic subunit subunit [13]. [13]. DesminDesmin is is a a tissue-specific tissue-specific ty typepe III intermediate filament filament expressed in skeletal and cardiaccardiac myocytes,myocytes, asas well well as as smooth smooth muscle muscle cells cells [6]. In[6]. skeletal In skeletal and cardiac and cardiac muscle, muscle, desmin desminsurrounds surrounds the Z- discs the Z- and discs links and the links myofibrillar the myofibrillar structure structure to the sarcolemma, to the sarcolemma, the nucleus, the nucleus,mitochondria mitochondria and lysosomes and lysosomes [6,14,15], while,[6,14,15], specifically while, specifically in cardiac muscle,in cardiac desmin muscle, also desminconnects also the connects Z- discs withthe Z- the discs intercalated with the intercalated discs [3] (Figure
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