Anti-GFRa-3 produced in rabbit, IgG fraction of antiserum

Catalog Number G9540

Product Description trochlear, superior salivatory, trigeminal, hypoglossal Anti-GFRa-3 (GDNFRg, RETL2, TrnR2, NTNR-a) is and weakly in the dorsal motor nucleus of the vagus produced in rabbit using a synthetic peptide and the ambiguus nucleus. The abducens nucleus is corresponding to amino acids from 347-360 of murine negative for both GFRa-1 and GFRa-2. GFRa-3 was GFRa-3 as immunogen.1 expressed only in the superior salivatory nucleus. GFRa-3 is expressed in developing and adult ganglia of Anti-GFRa-3 is specific for GFRa-3 by immunoblotting the PNS but was not detected in the CNS. (43 kDa). Anti-GFRa-3 reacts with mouse tissue lysates. Reagent Supplied in phosphate buffered saline, containing The GDNF family comprises glial cell line-derived 0.02% sodium azide. neurotrophic factor (GDNF) and the related proteins (NTN), (ART) and persephin (PSP), Protein concentration is ~0.5 mg/ml. which form a subgroup of the TGF-b superfamily. All four provide neuronal cell Precautions and Disclaimer protection and cell survival. In addition, GDNF and NTN This product is for R&D use only, not for drug, are also responsible for the development and survival household, or other uses. Please consult the Material of the enteric neurons, and NTN for parasympathetic Safety Data Sheet for information regarding hazards neurons. GDNF, a mesenchyme-derived signaling and safe handling practices. molecule, is also responsible for the promotion of ureteric branching in kidney development. NTN, ART, Storage/Stability and PSP are also expressed in the developing kidney, Antibody can be stored at 2-8 °C for three months and and NTN and PSP induce ureteric branching in vitro, at −20 °C for one year. As with all antibodies care but their true in vivo role in kidney morphogenesis is still should be taken to avoid repeated freeze thaw cycles. unclear. The members of the GDNF family, GDNF, Antibodies should not be exposed to prolonged high NTN, PSP, and ART have seven conserved cysteine temperatures. residues with similar spacing. Like the members of the family, the GDNF family belongs Product Profile structurally to the cysteine knot proteins. All Immunoblotting: recommended working dilution is bind to the p75 low-affinity receptor, but 1:500-1:1,000 by immunoblotting using mouse kidney, their ligand specificity is determined by liver or heart tissue lysates. tyrosine kinases. GDNF, NTN, PSP, and ART mediate their signals via a common receptor , Note: In order to obtain best results and assay Ret, but their ligand specificity is determined by a novel sensitivities of different techniques and preparations, class of glycosylphosphatidylinositol (GPI)-anchored we recommend determining optimal working dilutions proteins called the GDNF family receptor alpha by titration test. (GFRa). GDNF binds preferentially to GFRa-1, NTN to GFRa-2, ART to GFRa-3, and PSP to GFRa-4 as a co- References receptor to activate Ret. At early stages of development 1. Naveilhan, P., et al., Expression and regulation of GFRa-1 and GFRa-2 are expressed in the oculomotor, GFRalpha3, a glial cell line-derived neurotrophic factor family receptor. Proc. Natl. Acad. Sci. USA, facial and spinal accessory, and only GFRa-1 in the 95, 1295-1300 (1998). 2. Saarma, M., and Sariola, H., Other neurotrophic 6. Soler, R.M., et al., Receptors of the glial cell line- factors: glial cell line-derived neurotrophic factor derived neurotrophic factor family of neurotrophic (GDNF)., Microdc. Res. Tech., 45, 292-302 (1999). factors signal cell survival through the 3. Jing, S., et al., GFRalpha-2 and GFRalpha-3 are phosphatidylinositol 3-kinase pathway in spinal two new receptors for ligands of the GDNF family. cord motoneurons., J. Neurosci., 19, 9160-9169 J. Biol. Chem., 272, 33111-33117 (1997). (1999). 4. Baloh, R. H., et al., GFRalpha3 is an orphan 7. Mikaels, A., et al., A dynamic regulation of GDNF- member of the GDNF/neurturin/persephin receptor family recepotrs correlates with a specific trophic family. Proc. Natl. Acad. Sci. USA, 95, 5801-5806 dependency of cranial motor neuron (1998). subpopulations during development., Eur. J. 5. Worby, C. A., et al., Identification and Neurosci., 12, 446-456 (2000). characterization of GFRalpha-3 a novel co-receptor belonging to the glial cell line-derived neurotrophic RC,PHC 09-12-1 family. J. Biol. Chem., 273, 3502-3508 (1998).

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