Catalog # Aliquot Size

U229-911-05 3 x 5 nmol U229-911-20 3 x 20 nmol U229-911-50 3 x 50 nmol

UBE2L6 (UBCH8) siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # U229-911

Lot # Z2109-28

Specificity Formulation UBE2L6 (UBCH8) siRNAs are designed to specifically The siRNAs are supplied as a lyophilized powder and knock-down human UBE2L6 (UBCH8) expression. shipped at room temperature.

Product Description Reconstitution Protocol UBE2L6 (UBCH8) siRNA is a pool of three individual Briefly centrifuge the tubes (maximum RCF 4,000g) to synthetic siRNA duplexes designed to knock-down collect lyophilized siRNA at the bottom of the tube. human UBE2L6 (UBCH8) mRNA expression. Each siRNA is Resuspend the siRNA in 50 µl of DEPC-treated water 19-25 bases in length. The accession number is (supplied by researcher), which results in a 1x stock NM_004223. solution (10 µM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot Gene Aliases 1x stock solutions for storage. RIG-B, UBCH8 Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the UBE2E3 (UBCH9) U219-30H lyophilized and resuspended siRNAs are stored at or UBE2F Protein U220-30H below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2G1 (UBC7) Protein U221-30H µM can undergo up to 50 freeze-thaw cycles without UBE2H Protein U223-30H significant degradation. For long-term storage, it is UBE2I Protein U224-30H recommended that the siRNA is stored at -70oC. For most UBE2J1 Protein U225-30G favorable performance, avoid repeated handling and UBE2K (UBC1) Protein U227-30H multiple freeze/thaw cycles. UBE2L6 (UBCH8) Protein U229-30H

Scientific Background UBE2L6 (UBCH8) or -conjugating E2L 6 is a member of the E2 ubiquitin-conjugating enzyme family UBE2L6 (UBCH8) siRNA Set I siRNA duplexes targeted against three exon regions which is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Ubiquitination involves at Catalog # U229-911 least three classes of : ubiquitin-activating Lot # Z2109-28 enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and Packaging Specifications 2.5 nmol/tube for 3 x 5nmol ubiquitin-protein ligases (E3s) and the selective physical interaction between E2 and E3 enzymes forms the basis of Format Lyophilized powder Stability 1yr at -70oC from date of shipment specificity for functionally distinct E2-E3 combinations (1). Storage & Shipping The lyophilized powder is stable for at UBE2L6 and the ubiquitin-ligase SIAH1 physically interact least 4 weeks at room temperature. It is with and target FLT3-ITD for proteasomal degradation (2). recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, References siRNA stock solutions ≥2 µ M can 1. Kumar, S. et.al: Physical interaction between specific undergo up to 50 freeze-thaw cycles without significant degradation. For E2 and Hect E3 enzymes determines functional long-term storage, it is recommended cooperativity. J. Biol. Chem. 272: 13548-13554, 1997. that the siRNA is stored at -70oC. For 2. Buchwald, M. et al: Ubiquitin conjugase UBCH8 most favorable performance, avoid repeated handling and multiple targets active FMS-like tyrosine kinase 3 for freeze/thaw cycles. proteasomal degradation. Leukemia. 2010 Aug;24(8):1412-21.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.