Z-Leu-Leu-Tyr Fluoromethyl Ketone
Product Number C 9234 Storage Temperature -20 °C
Product Description Preparation Instructions Molecular Formula: C30H40FN3O6 Prepare stock 20 mM solutions in dry (³ 99.9 %) Formula Weight: 557.7 DMSO to maintain product stability. Also soluble in DMF. Z-Leu-Leu-Tyr Fluoromethyl Ketone (Z-LLY-FMK, Z = benzyloxycarbonyl) is an inhibitor of both Calpain and Storage/Stability Cathepsin L. Store at -20 °C. The product is reported to be stable at room temperature for one year in a desiccator. Calpains are cystolic calcium-activated neutral 1 proteases involved in cell apoptosis. Cathepsin L is a Store stock solutions at -20 °C for 6-8 months. lysosomal cysteine protease involved in intracellular 2 Allowing the material to warm to room temperature protein degradation. before use to ensure stability.
The related inhibitor Z-LLY-diazomethyl ketone References inactivated a calpain-like protease and prevented 1. Squire, M. K., et al., Calpain activation in WI-38 human fibroblasts cells from going into the apoptosis. J Cell Physiol., 159, 229-237 (1994). 3 S-phase during serum-stimulated growth. 2. Kopitar-Jerala, N., Anti-cathepsin L monoclonal antibodies that distinguish cathepsin L from FMK is a trapping group responsible for irreversible cathepsin V. Biol. Chem. 382, 867-870 (2001). inhibition but is non-cytotoxic. Inhibition occurs when 3. Zhang, W., et al., Inhibition of the growth of WI-38 the the FMK group covalently bonds to the –SH of an fibroblasts by benzyloxycarbonyl-Leu-Leu-Tyr adjacent cysteine residue on the target protein. diazomethyl ketone: evidence that cleavage of p53 by a calpain-like protease is necessary for G1 to S-phase transition. Oncogene, 14, 255-263 (1997).
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