ALOXE3 Antibody Cat. No.: 23-475
ALOXE3 Antibody
Immunofluorescence analysis of U2OS cells using ALOXE3 antibody (23-475) at dilution of 1:100. Blue: DAPI for nuclear staining.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of IMMUNOGEN: human ALOXE3 (NP_067641.2).
TESTED APPLICATIONS: IF, WB
WB: ,1:500 - 1:2000 APPLICATIONS: IF: ,1:50 - 1:200
POSITIVE CONTROL: 1) 22Rv1
2) MCF7
October 2, 2021 1 https://www.prosci-inc.com/aloxe3-antibody-23-475.html 3) Mouse skeletal muscle
4) Mouse testis
5) Rat intestine
PREDICTED MOLECULAR Observed: 81kDa WEIGHT:
Properties
PURIFICATION: Affinity purification
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.
Additional Info
OFFICIAL SYMBOL: ALOXE3
Hydroperoxide isomerase ALOXE3, Epidermis-type lipoxygenase 3, Epidermal LOX-3, e- ALTERNATE NAMES: LOX-3, eLOX-3, Hydroperoxy icosatetraenoate dehydratase, ALOXE3
GENE ID: 59344
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
This gene is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z- trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha BACKGROUND: (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene.
ANTIBODIES FOR RESEARCH USE ONLY.
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