IOTest CD58-PC5 PN IM3702 – 100 tests – 10 µL/test – Clone AICD58

For Research Use Only. Not For Use In Diagnostic Procedures.

SPECIFICITY Hybridoma Myeloma x63 Ag 8.653 x REAGENT PREPARATION The CD58 molecule (also known as Balb/c No reconstitution is necessary. This Lymphocyte-Function Associated Antigen 3 – Source Ascites fluid monoclonal antibody may be used directly LFA3) is a glycoprotein with a molecular Purification Ion exchange or affinity from the vial. Bring reagent to 18 – 25°C weight ranging from 55 to 70 kDa that chromatography prior to use. belongs to the immunoglobulin (Ig) Conjugation The Ig is conjugated to a PROCEDURE superfamily. The extracellular part of the tandem dye constituted of R-phycoerythrin This reagent is designed for Flow Cytometry. molecule consists of two Ig-like domains with covalently linked to cyanin 5.1 (PC5) at 0.5 – 5 Assay volume: 10 µL per 5 x 10 cells in one six potential N-linked glycosylation sites. The 1.5 moles of PC5 per mole of Ig. test, or per 100 µL whole blood. domain proximal to the cellular membrane is Excitation wavelength: 488 nm A wash is required to yield optimal results. a C2-type Ig-like domain whereas the Maximum emission wavelength: 670 nm domain distal to the cellular membrane is a Main emission color: Deep-red SELECTED RESEARCH REFERENCES V-type Ig-like domain and lacks the disulfide Buffer 2 mg/mL bovine serum albumin in 1. Klickstein, L.B., Springer, T.A., "CD58 bond. phosphate-buffered saline cluster report", 1995, Leucocyte Typing Alternative splicing gives rise to either containing 0.1% sodium azide. V, White Cell Differentiation Antigens. transmembrane or glycosyl-phosphatidyl- Kishimoto, T., et al, Eds., Garland APPLICATION inositol (GPI) anchored forms. Publishing, Inc., 1475-1476. The CD58 antigen is expressed on most Studies of expression 2. Takeuchi, E., Tanaka, T., Goda, K., hematopoietic cells such as T and B by flow cytometry. Miyasaka, M., "CD58 Workshop Panel lymphocytes, NK cells, monocytes, Studies of CD58 level of expression on bone report", 1997, Leucocyte Typing VI, granulocytes, circulating dendritic subsets, marrow sample in B-cell neoplasia (3). White Cell Differentiation Antigens. and erythrocytes. It is also expressed on STATEMENT OF WARNINGS Kishimoto, T., et al, Eds., Garland various non-hematopoietic cells, such as 1. This reagent contains 0.1% sodium Publishing, Inc., 414-415. fibroblasts, endothelial and epithelial cells. azide. Sodium azide under acid 3. Chen, J-S., Coustan-Smith, E., Suzuki, conditions yields hydrazoic acid, an T., Neale, G.A., Mihara, K., Pui, C-H., The CD58 antigen (LFA3) was first extremely toxic compound. Azide Campana, D., "Identification of novel described, together with LFA1 (CD11a / compounds should be flushed with markers for monitoring minimal residual CD18) and LFA2 (CD2), as participating in running water while being discarded. disease in acute lymphoblastic various immunological responses via effector These precautions are recommended to leukemia", 2001, Blood, 97, 2115-2120. lymphocyte adhesion and activation. It avoid deposits in metal piping in which PRODUCT AVAILABILITY functions as a triggering molecule and explosive conditions can develop. If skin IOTest CD58-PC5 Conjugated Antibodies mediates its signal through CD2, known as or eye contact occurs, wash excessively PN IM3702 – 100 tests – 10 µL/test the ligand of CD58, expressed on T with water. PC5 is licensed under patents 4,542,104 and lymphocytes and NK cells. 2. Specimens, samples and all material 4,520,110 Inhibitory studies have shown that the coming in contact with them should be AICD58 monoclonal antibody (mAb) doesn’t handled as if capable of transmitting For additional information in the USA, call block human erythrocyte rosetting (1, 2). infection and disposed of with proper 800-526-7694. The AICD58 mAb was assigned to the CD58 Outside the USA, contact your local th precautions. cluster of differentiation during the 5 Human 3. Never pipet by mouth and avoid contact Beckman Coulter representative. Leucocyte Differentiation Antigen (HLDA) of samples with skin and mucous TRADEMARKS international workshop, held in Boston, USA, membranes in 1993 (WS Code: S029, Section AS) (1). It IOTest is a registered trademark of 4. Do not use antibody beyond the Immunotech S.A. was used as a reference clone (WS Code: expiration date on the label. Ref. 28 Section AS, WS Code: 25 Section 5. Do not expose reagents to strong BP, and WS Code: 72 Section BP) during th during storage or incubation. Manufactured by: the 6 HLDA workshop held in Kobe, Japan, 6. Avoid microbial contamination of Immunotech, a Beckman Coulter Company in 1996 (2). reagents or incorrect results might 130, avenue de Lattre de Tassigny, B.P. 177 REAGENT occur. 13276 Marseille Cedex 9, France www.beckmancoulter.com IOTest CD58-PC5 Conjugated Antibodies STORAGE CONDITIONS AND STABILITY PN IM3702 – 100 tests - 10 µL/test This reagent is stable up to the expiration Clone AICD58 date when stored at 2 – 8°C. Do not freeze. Isotype IgG2a, mouse Minimize exposure to light. Immunogen PHA blast

Page 1 of 1 Insert No.: 3702EX030602 Vers. 01/ 14/06/02 AC-02-0325