Efficient Professional Protein and Antibody Platforms CBX8 Antibody Basic information: Catalog No.: UMA60409 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: Protein A affinity purified Useful Information: WB:1:500-1:1000 Applications: FC:1:50-1:200 Reactivity: Human Specificity: This antibody recognizes CBX8 protein. Immunogen: Recombinant protein Polycomb group (PcG) proteins form multiprotein complexes and play a role in gene silencing and Hox gene regulation by altering chromatin structure during transcription. CBX4 (chromobox homolog 4) and CBX8 (chromobox homolog 8), also known as PC2 or NBP16 and PC3 or RC1, respectively, are PcG proteins that show structural similarity to M33 and, like M33, bind the PcG protein RING1 through a conserved c-box motif located in the Description: C-terminus of RING1. However, CBX8 has only been shown to bind RING1 in vivo with covalently modified forms of RING1. CBX8 also interacts with the carboxy-terminus of AF9, a transcriptional activator implicated in the de- velopment of acute lukemias. CBX8 acts as a long range transcriptional si- lencer when targeted to a reporter gene by a heterologous DNA-binding domain. The human MPc3 gene maps to chromosome 17q25.3 and encodes a 389 amino acid protein. Uniprot: Q9HC52(Human) BiowMW: 43 kDa Buffer: 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data:

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of CBX8 on human CBX8 recombinant protein using anti-CBX8 antibody at 1/1,000 dilution.

Western blot analysis of CBX8 on HEK293 (1) and CBX8-hIgGFc transfected HEK293 (2) cell lysate using anti-CBX8 antibody at 1/1,000 dilution.

Flow cytometric analysis of Hela cells with CBX8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incuba- tion with primary antibody; red).

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]