Chemolithotrophic Processes in the Bacterial Communities on the Surface of Mineral-Enriched Biochars
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Microbiology of Barrier Component Analogues of a Deep Geological Repository
Microbiology of Barrier Component Analogues of a Deep Geological Repository by Rachel Beaver A thesis presented to the University of Waterloo in fulfillment of the thesis requirement for the degree of Master of Science in Biology Waterloo, Ontario, Canada, 2020 ©Rachel Beaver 2020 Author’s Declaration This thesis consists of material all of which I authored or co-authored: see Statement of Contributions included in the thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ii Statement of Contributions Chapter 2 The Tsukinuno bentonite sampling was coordinated by Erik Kremmer (NWMO). The Opalinus core was received from Niels Burzan and Rizlan Bernier-Latmani (École Polytechnique Fédérale de Lausanne, Switzerland). The Northern Ontario crystalline rock core sampling was coordinated by Jeff Binns (Nuclear Waste Management Organization). Sian Ford (McMaster University) swabbed the outer layer of the Northern Ontario crystalline rocK core and crushed the inner layer. Melody Vachon (University of Waterloo) assisted with the cultivation of anaerobic heterotrophs and SRB. iii Abstract Many countries are in the process of designing and implementing long-term storage solutions for used nuclear fuel. Like many of these countries, Canada is considering a deep geological repository (DGR) wherein used fuel bundles will be placed in copper-coated carbon steel used fuel containers encased in bentonite buffer boxes. Previously published research has simulated aspects of a DGR experimentally to identify DGR conditions that would prevent microbial activity. Although such microcosm-type experiments can observe microbial growth and activity over relatively limited time frames, a DGR will remain functional for at least a million years, and will be exposed to fluctuating environmental conditions. -
Metaproteogenomic Insights Beyond Bacterial Response to Naphthalene
ORIGINAL ARTICLE ISME Journal – Original article Metaproteogenomic insights beyond bacterial response to 5 naphthalene exposure and bio-stimulation María-Eugenia Guazzaroni, Florian-Alexander Herbst, Iván Lores, Javier Tamames, Ana Isabel Peláez, Nieves López-Cortés, María Alcaide, Mercedes V. del Pozo, José María Vieites, Martin von Bergen, José Luis R. Gallego, Rafael Bargiela, Arantxa López-López, Dietmar H. Pieper, Ramón Rosselló-Móra, Jesús Sánchez, Jana Seifert and Manuel Ferrer 10 Supporting Online Material includes Text (Supporting Materials and Methods) Tables S1 to S9 Figures S1 to S7 1 SUPPORTING TEXT Supporting Materials and Methods Soil characterisation Soil pH was measured in a suspension of soil and water (1:2.5) with a glass electrode, and 5 electrical conductivity was measured in the same extract (diluted 1:5). Primary soil characteristics were determined using standard techniques, such as dichromate oxidation (organic matter content), the Kjeldahl method (nitrogen content), the Olsen method (phosphorus content) and a Bernard calcimeter (carbonate content). The Bouyoucos Densimetry method was used to establish textural data. Exchangeable cations (Ca, Mg, K and 10 Na) extracted with 1 M NH 4Cl and exchangeable aluminium extracted with 1 M KCl were determined using atomic absorption/emission spectrophotometry with an AA200 PerkinElmer analyser. The effective cation exchange capacity (ECEC) was calculated as the sum of the values of the last two measurements (sum of the exchangeable cations and the exchangeable Al). Analyses were performed immediately after sampling. 15 Hydrocarbon analysis Extraction (5 g of sample N and Nbs) was performed with dichloromethane:acetone (1:1) using a Soxtherm extraction apparatus (Gerhardt GmbH & Co. -
CUED Phd and Mphil Thesis Classes
High-throughput Experimental and Computational Studies of Bacterial Evolution Lars Barquist Queens' College University of Cambridge A thesis submitted for the degree of Doctor of Philosophy 23 August 2013 Arrakis teaches the attitude of the knife { chopping off what's incomplete and saying: \Now it's complete because it's ended here." Collected Sayings of Muad'dib Declaration High-throughput Experimental and Computational Studies of Bacterial Evolution The work presented in this dissertation was carried out at the Wellcome Trust Sanger Institute between October 2009 and August 2013. This dissertation is the result of my own work and includes nothing which is the outcome of work done in collaboration except where specifically indicated in the text. This dissertation does not exceed the limit of 60,000 words as specified by the Faculty of Biology Degree Committee. This dissertation has been typeset in 12pt Computer Modern font using LATEX according to the specifications set by the Board of Graduate Studies and the Faculty of Biology Degree Committee. No part of this dissertation or anything substantially similar has been or is being submitted for any other qualification at any other university. Acknowledgements I have been tremendously fortunate to spend the past four years on the Wellcome Trust Genome Campus at the Sanger Institute and the European Bioinformatics Institute. I would like to thank foremost my main collaborators on the studies described in this thesis: Paul Gardner and Gemma Langridge. Their contributions and support have been invaluable. I would also like to thank my supervisor, Alex Bateman, for giving me the freedom to pursue a wide range of projects during my time in his group and for advice. -
Motiliproteus Sediminis Gen. Nov., Sp. Nov., Isolated from Coastal Sediment
Antonie van Leeuwenhoek (2014) 106:615–621 DOI 10.1007/s10482-014-0232-2 ORIGINAL PAPER Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment Zong-Jie Wang • Zhi-Hong Xie • Chao Wang • Zong-Jun Du • Guan-Jun Chen Received: 3 April 2014 / Accepted: 4 July 2014 / Published online: 20 July 2014 Ó Springer International Publishing Switzerland 2014 Abstract A novel Gram-stain-negative, rod-to- demonstrated that the novel isolate was 93.3 % similar spiral-shaped, oxidase- and catalase- positive and to the type strain of Neptunomonas antarctica, 93.2 % facultatively aerobic bacterium, designated HS6T, was to Neptunomonas japonicum and 93.1 % to Marino- isolated from marine sediment of Yellow Sea, China. bacterium rhizophilum, the closest cultivated rela- It can reduce nitrate to nitrite and grow well in marine tives. The polar lipid profile of the novel strain broth 2216 (MB, Hope Biol-Technology Co., Ltd) consisted of phosphatidylethanolamine, phosphatidyl- with an optimal temperature for growth of 30–33 °C glycerol and some other unknown lipids. Major (range 12–45 °C) and in the presence of 2–3 % (w/v) cellular fatty acids were summed feature 3 (C16:1 NaCl (range 0.5–7 %, w/v). The pH range for growth x7c/iso-C15:0 2-OH), C18:1 x7c and C16:0 and the main was pH 6.2–9.0, with an optimum at 6.5–7.0. Phylo- respiratory quinone was Q-8. The DNA G?C content genetic analysis based on 16S rRNA gene sequences of strain HS6T was 61.2 mol %. Based on the phylogenetic, physiological and biochemical charac- teristics, strain HS6T represents a novel genus and The GenBank accession number for the 16S rRNA gene T species and the name Motiliproteus sediminis gen. -
Aquabacterium Gen. Nov., with Description of Aquabacterium Citratiphilum Sp
International Journal of Systematic Bacteriology (1999), 49, 769-777 Printed in Great Britain Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system Sibylle Kalmbach,’ Werner Manz,’ Jorg Wecke2 and Ulrich Szewzyk’ Author for correspondence : Werner Manz. Tel : + 49 30 3 14 25589. Fax : + 49 30 3 14 7346 1. e-mail : [email protected]. tu-berlin.de 1 Tech nisc he U nive rsit ;it Three bacterial strains isolated from biofilms of the Berlin drinking water Berlin, lnstitut fur system were characterized with respect to their morphological and Tec hn ischen Umweltschutz, Fachgebiet physiological properties and their taxonomic position. Phenotypically, the Okologie der bacteria investigated were motile, Gram-negative rods, oxidase-positive and Mikroorganismen,D-l 0587 catalase-negative, and contained polyalkanoates and polyphosphate as Berlin, Germany storage polymers. They displayed a microaerophilic growth behaviour and 2 Robert Koch-lnstitut, used oxygen and nitrate as electron acceptors, but not nitrite, chlorate, sulfate Nordufer 20, D-13353 Berlin, Germany or ferric iron. The substrates metabolized included a broad range of organic acids but no carbohydrates at all. The three species can be distinguished from each other by their substrate utilization, ability to hydrolyse urea and casein, cellular protein patterns and growth on nutrient-rich media as well as their temperature, pH and NaCl tolerances. Phylogenetic analysis, based on 165 rRNA gene sequence comparison, revealed that the isolates are affiliated to the /I1 -subclass of Proteobacteria. The isolates constitute three new species with internal levels of DNA relatedness ranging from 44.9 to 51*3O/0. -
Supplementary File 1
Supplementary File 1. Unlocking survival mechanisms for metal and oxidative stress in the extremely acidophilic, halotolerant Acidihalobacter genus Himel Nahreen Khaleque1,5, Homayoun Fathollazadeh1, Carolina González2,4, Raihan Shafique1, Anna H. Kaksonen5, David S. Holmes2,3,4 and Elizabeth L.J. Watkin1* 1School of Pharmacy and Biomedical Sciences, Curtin University, Perth, Australia; 2Center for Bioinformatics and Genome Biology, Fundacion Ciencia y Vida, Santiago, Chile; 3Universidad San Sebastian, Santiago Chile; 4Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago, Chile; and 5CSIRO Land and Water, Floreat, Australia *Correspondence: [email protected]; Tel.: (+61 8 92662955) Table S1. BLASTx analysis of the Acidihalobacter yilgarnensis F5T operon containing mobile genetic elements and copper resistance genes. Next best hits in red = genes with similarity to orthologs in acidophiles; green = genes with similarity to orthologs in neutrophilic halophilic and halotolerant organisms or to other non-acidophiles, black = unique to Acidihalobacter, blue = genes with similarity to orthologs found across the domain Bacteria. peg. RAST annotation NCBI annotation Ident (%) to NCBI accession Next best hit (NBH) Ident NCBI accession No Acidhalocbacter (%) to sp. NBH 2773 FIG00761799: DUF4396 domain- 100% WP_070080076.1 DUF4396 domain- 55.02% WP_110138179.1 membrane protein containing protein containing protein CDS [Acidihalobacter [Acidiferrobacter sp. prosperus] SPIII_3] 2774 Hypothetical protein hypothetical -
Leptothrix Cholodnii
This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Article Cite This: ACS Nano 2020, 14, 5288−5297 www.acsnano.org Polyfunctional Nanofibril Appendages Mediate Attachment, Filamentation, and Filament Adaptability in Leptothrix cholodnii ‡ ⊥ † ⊥ ∇ † ‡ § Tatsuki Kunoh,*, , Kana Morinaga, , Shinya Sugimoto, Shun Miyazaki, Masanori Toyofuku, , ∥ ‡ § ‡ § Kenji Iwasaki, Nobuhiko Nomura,*, , and Andrew S. Utada*, , ‡ † § ∥ Faculty and Graduate School of Life and Environmental Sciences, Microbiology Research Center for Sustainability, and Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan ∇ Department of Bacteriology and Jikei Center for Biofilm Research and Technology, The Jikei University School of Medicine, 3-25-8, Nishi-Shimbashi, Minato-ku, Tokyo 105-8461, Japan *S Supporting Information ABSTRACT: Leptothrix is a species of Fe/Mn-oxidizing bacteria known to form long filaments composed of chains of cells that eventually produce a rigid tube surrounding the filament. Prior to the formation of this brittle microtube, Leptothrix cells secrete hair-like structures from the cell surface, called nanofibrils, which develop into a soft sheath that surrounds the filament. To clarify the role of nanofibrils in filament formation in L. cholodnii SP-6, we analyze the behavior of individual cells and multicellular filaments in high-aspect ratio microfluidic chambers using time-lapse and intermittent in situ fluorescent staining of nanofibrils, complemented with atmospheric scanning electron microscopy. We show that in SP-6 nanofibrils are important for attachment and their distribution on young filaments post-attachment is correlated to the directionality of filament elongation. -
Genes for Degradation and Utilization of Uronic Acid-Containing Polysaccharides of a Marine Bacterium Catenovulum Sp
Genes for degradation and utilization of uronic acid-containing polysaccharides of a marine bacterium Catenovulum sp. CCB-QB4 Go Furusawa, Nor Azura Azami and Aik-Hong Teh Centre for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas, Penang, Malaysia ABSTRACT Background. Oligosaccharides from polysaccharides containing uronic acids are known to have many useful bioactivities. Thus, polysaccharide lyases (PLs) and glycoside hydrolases (GHs) involved in producing the oligosaccharides have attracted interest in both medical and industrial settings. The numerous polysaccharide lyases and glycoside hydrolases involved in producing the oligosaccharides were isolated from soil and marine microorganisms. Our previous report demonstrated that an agar-degrading bacterium, Catenovulum sp. CCB-QB4, isolated from a coastal area of Penang, Malaysia, possessed 183 glycoside hydrolases and 43 polysaccharide lyases in the genome. We expected that the strain might degrade and use uronic acid-containing polysaccharides as a carbon source, indicating that the strain has a potential for a source of novel genes for degrading the polysaccharides. Methods. To confirm the expectation, the QB4 cells were cultured in artificial seawater media with uronic acid-containing polysaccharides, namely alginate, pectin (and saturated galacturonate), ulvan, and gellan gum, and the growth was observed. The genes involved in degradation and utilization of uronic acid-containing polysaccharides were explored in the QB4 genome using CAZy analysis and BlastP analysis. Results. The QB4 cells were capable of using these polysaccharides as a carbon source, and especially, the cells exhibited a robust growth in the presence of alginate. 28 PLs and 22 GHs related to the degradation of these polysaccharides were found in Submitted 5 August 2020 the QB4 genome based on the CAZy database. -
Succession and Cazyme Expression of Marine Bacterial
bioRxiv preprint doi: https://doi.org/10.1101/2020.12.08.416354; this version posted December 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Sweet and magnetic: Succession and CAZyme expression of marine bacterial communities encountering a mix of alginate and pectin particles Carina Bunse1,2,*, Hanna Koch3,4,*, Sven Breider3, Meinhard Simon1,3 and Matthias Wietz2,3# 1Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg, Germany 2Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Germany 3Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Germany 4Department of Microbiology, Radboud University Nijmegen, The Netherlands *These authors contributed equally to this work. #Corresponding author HGF-MPG Joint Research Group for Deep-Sea Ecology and Technology Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Am Handelshafen 12 27570 Bremerhaven Germany Email: [email protected] Telephone +49-471-48311454 bioRxiv preprint doi: https://doi.org/10.1101/2020.12.08.416354; this version posted December 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. ABSTRACT Polysaccharide particles are an important nutrient source and microhabitat for marine bacteria. However, substrate-specific bacterial dynamics in a mixture of particle types with different polysaccharide composition, as likely occurring in natural habitats, are undescribed. -
Isolation and Characterization of a Novel Agar-Degrading Marine Bacterium, Gayadomonas Joobiniege Gen, Nov, Sp
J. Microbiol. Biotechnol. (2013), 23(11), 1509–1518 http://dx.doi.org/10.4014/jmb.1308.08007 Research Article jmb Isolation and Characterization of a Novel Agar-Degrading Marine Bacterium, Gayadomonas joobiniege gen, nov, sp. nov., from the Southern Sea, Korea Won-Jae Chi1, Jae-Seon Park1, Min-Jung Kwak2, Jihyun F. Kim3, Yong-Keun Chang4, and Soon-Kwang Hong1* 1Division of Biological Science and Bioinformatics, Myongji University, Yongin 449-728, Republic of Korea 2Biosystems and Bioengineering Program, University of Science and Technology, Daejeon 305-350, Republic of Korea 3Department of Systems Biology, Yonsei University, Seoul 120-749, Republic of Korea 4Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea Received: August 2, 2013 Revised: August 14, 2013 An agar-degrading bacterium, designated as strain G7T, was isolated from a coastal seawater Accepted: August 20, 2013 sample from Gaya Island (Gayado in Korean), Republic of Korea. The isolated strain G7T is gram-negative, rod shaped, aerobic, non-motile, and non-pigmented. A similarity search based on its 16S rRNA gene sequence revealed that it shares 95.5%, 90.6%, and 90.0% T First published online similarity with the 16S rRNA gene sequences of Catenovulum agarivorans YM01, Algicola August 22, 2013 sagamiensis, and Bowmanella pacifica W3-3AT, respectively. Phylogenetic analyses demonstrated T *Corresponding author that strain G7 formed a distinct monophyletic clade closely related to species of the family Phone: +82-31-330-6198; Alteromonadaceae in the Alteromonas-like Gammaproteobacteria. The G+C content of strain Fax: +82-31-335-8249; G7T was 41.12 mol%. -
Metagenomics Unveils the Attributes of the Alginolytic Guilds of Sediments from Four Distant Cold Coastal Environments
Metagenomics unveils the attributes of the alginolytic guilds of sediments from four distant cold coastal environments Marina N Matos1, Mariana Lozada1, Luciano E Anselmino1, Matías A Musumeci1, Bernard Henrissat2,3,4, Janet K Jansson5, Walter P Mac Cormack6,7, JoLynn Carroll8,9, Sara Sjöling10, Leif Lundgren11 and Hebe M Dionisi1* 1Laboratorio de Microbiología Ambiental, Centro para el Estudio de Sistemas Marinos (CESIMAR, CONICET), Puerto Madryn, U9120ACD, Chubut, Argentina 2Architecture et Fonction des Macromolécules Biologiques, CNRS, Aix-Marseille Université, 13288 Marseille, France 3INRA, USC 1408 AFMB, F-13288 Marseille, France 4Department of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia 5Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA 6Instituto Antártico Argentino, Ciudad Autónoma de Buenos Aires, C1064ABR, Argentina 7Instituto Nanobiotec, CONICET- Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, C1113AAC, Argentina 8Akvaplan-niva, Fram – High North Research Centre for Climate and the Environment, NO- 9296 Tromsø, Norway 9CAGE - Centre for Arctic Gas Hydrate, Environment and Climate, UiT The Arctic University of Norway, N-9037 Tromsø, Norway 10School of Natural Sciences and Environmental Studies, Södertörn University, 141 89 Huddinge, Sweden 11Stockholm University, SE-106 91 Stockholm, Sweden This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as an ‘Accepted Article’, doi: 10.1111/1462-2920.13433 This article is protected by copyright. All rights reserved. Page 2 of 41 Running title: Alginolytic guilds from cold sediments *Correspondence: Hebe M. -
Insights Into the Biology of Acidophilic Members of the Acidiferrobacteraceae Family Derived from Comparative Genomic Analyses
Research in Microbiology 169 (2018) 608e617 Contents lists available at ScienceDirect Research in Microbiology journal homepage: www.elsevier.com/locate/resmic Insights into the biology of acidophilic members of the Acidiferrobacteraceae family derived from comparative genomic analyses Francisco Issotta a, b, Ana Moya-Beltran a, b, Cristobal Mena c, Paulo C. Covarrubias a, b, 1, ** Christian Thyssen d,Soren€ Bellenberg d, Wolfgang Sand d, e, f, Raquel Quatrini a, , 2, * Mario Vera c, g, a ~ Fundacion Ciencia y Vida, Avenida Zanartu~ 1482, Nunoa,~ 7780272, Santiago, Chile b Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile c Instituto de Ingeniería Biologica y Medica, Escuelas de Ingeniería, Medicina y Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Av. Vicuna~ Mackenna 4860, Macul, 7820486, Santiago, Chile d Universitat€ Duisburg Essen, Biofilm Centre, Aquatische Biotechnologie, Universitatsstr.€ 5, 45141, Essen, Germany e College of Environmental Science and Engineering, Donghua University, 2999 North Ren Min Rd., Song Jiang District, Shanghai, 201620, PR China f Technische Universitat€ Bergakademie Freiberg, Institut für Biowissenschaften, Leipziger Str. 29, 09599, Freiberg, Germany g Departamento de Ingeniería Hidraulica y Ambiental, Escuela de Ingeniería, Pontificia Universidad Catolica de Chile, Av. Vicuna~ Mackenna 4860, Macul, 7820486, Santiago, Chile article info abstract Article history: The family Acidiferrobacteraceae (order Acidiferrobacterales) currently contains Gram negative, neutro- Received 8 April 2018 philic sulfur oxidizers such as Sulfuricaulis and Sulfurifustis, as well as acidophilic iron and sulfur oxidizers Accepted 8 August 2018 belonging to the Acidiferrobacter genus. The diversity and taxonomy of the genus Acidiferrobacter has Available online 22 August 2018 remained poorly explored. Although several metagenome and bioleaching studies have identified its presence worldwide, only two strains, namely Acidiferrobacter thiooxydans DSM 2932T, and Acid- Keywords: iferrobacter spp.