Finnigan LTQ

System Specifications Environment International Offices MS/MS Sensitivity • System averages 2300 W (8000 Btu/h) output when considering air conditioning Electrospray Ionization (ESI) – A loop needs Australia injection of 2 µL of a 125 fg/µL solution • Operating environment must be 15-27°C Tel. (61) 2 9898 9000 of reserpine (250 femtograms, 0.375 (59-80°F) and relative humidity must be Austria femtomoles total sample) at a flow of 4-80% with no condensation Tel. (43) 1 333 50340 400 µL/min of 50% isopropyl alcohol/50% • Optimum operating temperature is Belgium water will produce a minimum signal-to- 18-21°C (65-70°F) noise ratio of 50:1, for the transition of Tel. (32) 2 482 3030 Dimensions/Weight the isolated protonated molecular ion Canada × × × × at m/z 609 to the largest two product ions, • MS: 56 cm 79 cm 59 cm (h w d) Tel. (905) 712 2258 when the mass spectrometer is operated • MS: ~120 kg Canada only at unit resolution in the full-scan MS/MS • Two roughing pumps: 38.6 kg each Tel. (800) 721 4260 mode, scanning the product ion spectrum Central and from m/z 165 – 615. Performance Specifications South America Tel. (01) 512 251 1530 Mass Range Atmospheric Pressure Chemical France • m/z 50 – 2000 Ionization (APCI) – A loop injection of Tel. (33) 1 60 92 48 00 2 µL of a 125 fg/µL solution of reserpine Scan Power Germany (250 femtograms, 0.375 femtomoles total •MSn, for n = 1 through 10 Tel. (49) 6103 4080 sample) at a flow of 400 µL/min of 50% • Contact Closure Italy isopropyl alcohol/50% water will produce • Start In/Out Tel. (39) 02 950 591 a minimum signal-to-noise ratio of 50:1, • Start Out is programmable for the transition of the isolated protonated Japan molecular ion at m/z 609 to the largest two Analog Inputs Tel. (81) 45 453 9100 product ions, when the mass spectrometer • One (1) analog Input (0-1 V) The Netherlands Tel. (31) 76 5878 722 is operated at unit resolution in the full- • One (1) analog Input (0-10 V) scan MS/MS mode, scanning the product People’s Republic ion spectrum from m/z 165 – 615. of China Tel. (86) 10 6621 0839

Installation Requirements Spain Tel. (34) 91 657 4930 Power Sweden • One 230 Vac ±10.0%, 15 Amps, 50/60 Hz, Tel. (46) 8 556 468 00 single phase, with earth ground dedicated to the instrument Switzerland • 120 or 230 Vac single phase, with earth Tel. (41) 61487 8400 ground for the data system United Kingdom Gas Tel. (44) 1 442 233555 • One high-purity (99% pure, flow rate United States 15 L/min) nitrogen gas supply for the Tel. (01) 800 532 4752 API source • One ultra high-purity helium gas supply (99.998% pure) with less than 1 ppm each of water, oxygen, and total hydrocarbons for the mass analyzer

www.thermo.com/finnigan DS1323 6/03

©2003 Thermo Electron Corporation. All rights reserved. Intel and Pentium are registered trademarks of Intel Corporation, Mass Frontier is a trademark of HighChem, Ltd., Microsoft and Windows are registered trademarks of Microsoft Corporation, TurboSEQUEST is a trademark and SEQUEST is a registered trademark of the University of Washington. All other trademarks are the property of Thermo Electron Corporation and its subsidiaries. We make no warranties, expressed or implied, in this product summary, and information is subject to change without notice. Product Specifications suite within ™ These technology ® ™ improvements deliver the performance and easy-to-use features that accelerate the identification and quantification of analytes at low levels in applications such as proteomics, metabolism, and environmental studies. the cross platform data acquisition software, Xcalibur. Unrivaled sensitivity and fast scanning serve to maximize the power of the advanced Data Dependent Control • instrument Measure n • Detect • source, allowing simple tool- ™ Analyze Analyze less switching of ionization probes. The exceptional ion transmission achieved with the Ion Max source, combined with the increased ion storage capacity of the linear ion trap, novel ion ejection and makes the Finnigan detection technology, the most sensitive LC/MS LTQ available today. The Finnigan LTQ is the latest innovation in The Finnigan LTQ the well-established Finnigan ion trap mass spectrometers. Based on novel design features including a hyperbolic segmented linear ion trap, dual detectors, radial ion ejection, and state-of-the-art electronics, redefines performance. the Finnigan LTQ The culmination of these features enables more experiments, leading to greater coverage on less sample in less time. incorporates the rugged The Finnigan LTQ Ion Max The Finnigan LTQ is a high precision linear ion linear ion precision is a high LTQ The Finnigan unparalleled that delivers spectrometer trap mass fast scanning. and sensitivity ™ LTQ ™

• Advanced Data Dependent Suite • Scan Speed Increased • Detectors Dual • Ion Max Source Universal • Sensitivity Unparalleled

Technology you can trust Finnigan Product Specifications

Hardware Features Detection System Ion Max API Source • Patented dual conversion dynode detector • Two off-axis continuous dynode electron • Enhanced sensitivity and ruggedness multipliers with extended dynamic range • Sweep Gas™ reduces chemical noise • Digital electronic noise discrimination • 60° interchangeable ion probe orientation • High-efficiency radial ion ejection • Removable metal ion capillary tube provides vent-free maintenance Integrated Divert Valve Transfer Ion Optics • Fully-automated data system control enables user to switch the • Advanced multipole ion guides solvent front, gradient end point and • Split voltage quadrupole lens any other portion of the HPLC run reduces noise to waste • High stability and ion transmission • User-definable default state of the efficiency valve, either “to waste” or “to source” 2D Linear Quadrupole Ion Trap Integrated Syringe Pump Mass Spectrometer • Syringe Pump allows automated infusion • Analyzer dimensions optimized for under data system control ultimate performance Options • Regulated helium flow for stable performance • ESI source compatible with liquid flow • Automatic system calibration and rates of <1 µL/min to 1 mL/min, without optimization splitting • Radial ion ejection • APCI/APPI source compatible with liquid flow rates of 50 µL/min to 2 mL/min, Vacuum System without splitting • Differentially-pumped vacuum system • APCI source compatible with liquid flow to 10-5 Torr rates of 50 µL/min to 2 mL/min, without • Split-flow turbomolecular pump controls splitting vacuum in three regions • Metal needle option for high and • Dual rotary vacuum pump configuration low-flow analyses • High-vacuum machined aluminum • 96-position sample plate AP MALDI for analyzer chamber automated and manual data acquisition • NanoSpray source supports both static and dynamic nanospray experiments, compatible with liquid flow rates of 50 nL/min* to 2 µL/min

*Lower limit is dependent on gauge of needle used Finnigan LTQ

Software Features 461.0 Data System • Xcalibur processing and instrument control software •LCQUAN™ quantification package • Microsoft® Office XP software package • Microsoft Windows® XP operating system 537.2 • High-performance PC with Intel® 425.3 Pentium® microprocessor 650.8 • High-resolution LCD color monitor 416.5 749.0 Scan Functions 630 1 404 6 • Full-scan feature provides full-scan mass spectra for sensitive analyses and produces spectra for rapid screening of unknown compounds • Selected Ion Monitoring (SIM) monitors LC-MS analysis of an enzyme digest of 4 fmol of myoglobin. selected ions for target compound analysis spectrum, generating more structurally • Ion Mapping Browser software for • Full-scan MS/MS produces full-scan informative spectra viewing data generated by Ion product ion spectra at sensitivities ™ Mapping experiments higher than any ion trap mass • Normalized Collision Energy spectrometer compensates for the mass-dependent • Data Dependent Ion Tree performs energy deposition characteristics of MSn experiments on up to 25 species • Selected Reaction Monitoring (SRM) ion trap mass spectrometers in MS/MS for a traditional LC/MS/MS quantitative • Data Dependent Zoom Map generates experiments, providing reproducible data sequential MS/MS experiments using analytical experiment from instrument to instrument n a ZoomScan for charge state determina- •MS for multi-stage MS experiments tion prior to each MS/MS experiment to probe the structure of ions Advanced Data Dependent •MSn Browser software for viewing data ™ Experiments • ZoomScan, a high-resolution, full-range generated by Data Dependent Ion Tree scan to resolve isotopic envelopes • Data Dependent features trigger and Ion Mapping experiments which is often used for charge state acquisition of MSn spectra only when determination of peptides and ionization a compound of interest is detected Optional Application-Specific state determination of organometallics • Isotopic Data Dependent scanning soft- Software • Ultra ZoomScan for ultimate resolution ware triggers MS/MS scanning only • BioWorks™/SEQUEST®/TurboSEQUEST™ – • TurboScan,™ an ultra-fast scan to improve when a specified isotopic pattern is protein identification tools signal-to-noise and sampling rate detected. Performs MS/MS only when a • Mass Frontier™ – spectral interpretation user-defined isotopic pattern is detected Exclusive Technologies and classification software to identify • Triple Play determines the charge state unknowns • Unique, patented Automatic Gain Control and MS/MS scan range of a multiply • Metabolite ID – rapid review and (AGC) ensures that the ion trap is always charged ion reporting of drug metabolism data filled with the optimum number of ions • Nth Order Triple Play allows the number ™ for any scan type • DeNovoX – automatically sequences of ions undergoing a Triple Play to be unknown peptides • Dynamic Exclusion™ allows acquisition defined n of MS/MS and MS spectra from lower • Ion Mapping™ automatically generates intensity ion species a 3-dimensional MS/MS map, yielding • WideBand Activation™ fragments the product ion, precursor ion, and neutral water loss ion within an MS/MS loss information