Phase-Shifting Mechanisms in the Mammalian Circadian System: New Light on the Carbachol Paradox
Total Page:16
File Type:pdf, Size:1020Kb
The Journal of Neuroscience, April 1993, 73(4): 1454-1459 Phase-shifting Mechanisms in the Mammalian Circadian System: New Light on the Carbachol Paradox Christopher S. Colwell, Claire M. Kaufman, and Michael Menaker NSF Center for Biological Timing, Department of Biology, University of Virginia, Charlottesville, Virginia 22901 A variety of evidence now suggests that excitatory amino In mammals, the suprachiasmaticnucleus (SCN) functions acid receptors mediate the effects of light on the circadian as the dominant pacemakerof the circadian system. The effects system of mammals. However, the ACh agonist carbachol of light on the SCN are mediated by unknown photoreceptors is the only agent that has been reported to “mimic” the located in the retina (Nelson and Zucker, 1981; Foster et al., phase-shifting effects of light in vivo. Because the other 1991) that project to the hypothalamus, at least in part, via a published evidence for the involvement of ACh in light-me- monosynaptic fiber tract known asthe retinohypothalamic tract diated phase shifts is weak, we have referred to this situation (RHT) (Moore, 1983). as “the carbachol paradox.” In the present study, we found A variety of evidence suggestsan excitatory amino acid (EAA) that the administration of NMDA receptor antagonists could is a transmitter at the RHTSCN synaptic connection. There is prevent carbachol-induced phase shifts of the circadian evidence that both the NMDA and non-NMDA receptor sub- rhythm of wheel-running activity recorded from the hamster. classesof EAA receptors are involved. Electrophysiological In addition, we found that carbachol-induced phase shifts, studies using an in vitro hypothalamic slice preparation that unlike those produced by light, are not accompanied by in- contains the SCN suggesta role for both receptor types (Shibata duction of Fos-like immunoreactivity in the suprachiasmatic et al., 1986; Cahill et al., 1989; Mason and Rusak, 1990; Ito et nucleus (SCN). Our data are simply explained by the as- al., I99 1; Kim and Dudek, 199 1). For example, Kim and Dudek sumption that the intraventricular administration of carbach- (199 1) found that EPSPsrecorded intracellularly in the SCN in 01 causes phase shifts through a pathway distinct from that responseto optic nerve stimulation wereblocked by both NMDA of light. Alternatively, if carbachol is acting via the light input and non-NMDA receptor antagonists. Recent anatomical evi- pathway, then it must do so by a mechanism independent dence suggeststhat EAA-immunoreactive fibers innervate the of Fos induction in the SCN. In either case, elucidating the SCN (Moffett et al., 1990; Van den Pol, 1991). Finally, phar- mechanisms by which carbachol acts in the circadian system macological studies have implicated both NMDA and non- may provide novel insights into the cellular events by which NMDA receptor types in the photic regulation of the circadian phase shifts are generated. system of rodents (Colwell et al., 1990, 1991; Rea et al., 1991; [Key words: hamster, circadian rhythm, excitatory amino Takeuchi et al., 1991; Colwell and Menaker, 1992). acids, ACh, CCP (2-carboxypiperazin-4-yl)-propyl- 1 -phos- It has been suggestedthat ACh may also play a role in the phonic acid), dizocilpine, carbachol] light-input pathway. The intraventricular administration of the ACh agonist carbachol causesphases shifts that have been re- Circadian rhythms are endogenouslygenerated oscillations with ported to mimic the effects of light on the mammalian circadian periods of approximately 24 hr. The physiological system re- system(Zatz and Brownstein, 1979; Zatz and Herkenham, 1981; sponsiblefor these rhythms can be modeled in three parts: (1) Earnest and Turek, 1983, 1985; Wee et al., 1992; but also see an input pathway by which environmental signals are trans- Meijer et al., 1988, for alternative view). Additionally, meca- mitted to (2) a pacemaker, which is coupled to the processesit mylamine, an antagonist to the ion channelsactivated by ACh, controls by (3) an output pathway. In order to function adap- has been reported to prevent light-induced phaseshifts (Keefe tively, the circadian systemmust be synchronized (or entrained) et al., 1987). Further, electrophysiological studiesindicate that with appropriate phaseto the 24 hr period of the environment some SCN neuronsshow similar responsesto light and cholin- in which the organism lives. The daily light-dark cycle, acting ergic agents(Nishino and Koizumi, 1977; Miller et al., 1987). through light-induced advances and delays of the endogenous Finally, the ACh content of the SCN increasesafter a light pulse oscillation, is the dominant environmental signal responsible (Murakami et al., 1984).Nevertheless, the neuroanatomicalsite for this entrainment. A major goal of circadian rhythm research of action and pharmacological specificity of carbachol remain is to understand the cellular mechanismsby which light signals unclear (seeRusak and Bina, 1990, for discussion). phaseshift endogenouscircadian oscillators. The protein Fos (the product of the c-fos gene) is induced in neuronsin responseto a number of stimuli including EAAs and Received May 26, 1992; revised Sept. 30, 1992; accepted Oct. 6, 1992. carbachol (Morgan and Curran, 1991). Recent work has shown We thank Dr. N. Wayne for comments on an early draft of the manuscript. that light causesan increasein Fos-like immunoreactivity (Fos- This work was sunoorted bv NIH Grant HD13162 to M.M. and NIMH Grant LI) and c-fos mRNA in the SCN (Rea, 1989;Aronin et al., 1990; MH09987-01 to C%.C. C.M:K. and C.S.C. were also supported by the NSF Center for Biological Timing. C.S.C. is the recipient ofthe FESN fellowship on “Circadian Earnestet al., 1990; Komhauser et al., 1990; Rusak et al., 1990; Rhythms.” Colwell and Foster, 1992). In addition, the induction of Fos in Correspondence should be addressed to C. S. Colwell, Mental Retardation Re- search Center, University of California at Los Angeles, 760 Westwood Plaza, Los the SCN by light is correlated in both its phasedependence and Angeles, CA 90024-l 759. threshold with the ability of the samelight treatment to phase Copyright 0 1993 Society for Neuroscience 0270-6474/93/131454-06$05.00/O shift the circadian oscillator (Komhauser et al., 1990). Fur- The Journal of Neuroscience, April 1993, 13(4) 1455 thermore, EAA receptor antagonists inhibit the photic induction method for calculating phase shifts was the same as has been reported of Fos in the SCN (Abe et el., 1991, 1992; Colwell and Foster, elsewhere (Takahashi et al., 1984). The phase-shifting effects of a treat- ment were considered to be significant when the 95% confidence interval 1991; Rea et al., 199 1) while the administration of the EAA of the group mean did not overlap zero. Differences between treatment agonist NMDAcan cause an induction of Fos in the SCN (Ebling groups were evaluated using a Kruskal-Wallis one-way analysis of vari- et al., 199 1). Although it is not clear whether Fos plays a causal ance, followed by a Mann-Whitney U test where appropriate. Values role in light-induced phase shifts of the circadian system, pre- were considered significantly different if P < 0.05. In the text, values are shown as means +Z SEM; and phase advances are shown as positive vious work does suggest that Fos-induction can be used as a values while phase delays are shown as negative values. cellular marker of photic input to the SCN. Tissuepreparation and immunocytochemistry. For perfusion, animals To elucidate the effects of carbachol and the possible role of were removed from their light-tight boxes and anesthetized with a lethal ACh in mediating light-induced phase shifts of the circadian dose of halothane by inhalation in darkness. Then, under dim red light, system, we conducted experiments to determine whether the the hamsters were perfused intracardially first with 30-40 ml of phys- iological saline (0.9% w/v) containing 150 IU of heparin/ 10 ml, followed administration of the NMDA receptor antagonists dizocilpine by 300 ml of 2% w/v formaldehyde in 0.1 M phosphate-buffered saline (MK-801) and 3-(2-carboxypiperazin-4-yl)-propyl-l-phos- (PBS; pH 7.2-7.4) with picric acid (15%). After perfusion, the brains phonic acid (CPP) could prevent carbachol-induced phase shifts were removed and postfixed overnight at 4°C. Serial frontal sections of the circadian rhythm of wheel-running activity. We then were cut at 50 pm into PBS using a cryostat. Free-floating sections were transferred to a solution of normal goat asked whether intraventricular injections of carbachol caused serum ( 1:30 in PBS with 2% Triton) for 30 min then directly into primary induction of Fos-LI in the SCN. We reasoned that if ACh acts antiserum. The Fos antiserum used in this study was an anti-Fos at any point along the light input pathway to the circadian (4- 17) rabbit polyclonal antiserum purchased from Oncogene Science system then EAA receptor antagonists should prevent carbach- (Uniondale. NY). The antibodv was used at a dilution of 1:250 for 72 ol-induced phase shifts and carbachol itself should cause an hr at 4°C. The sites of the antibddy:antigen binding were visualized with an avidin-biotin-peroxidase procedure (Elite ABC kit, Vector Labs, induction of Fos-LI in the SCN. Burlingame, CA). Sections were washed in Tris buffer (pH 7.2, 15 min) before incubation for 6 min in 0.025% diaminobenzidine (DAB) con- taining 0.003% (w/v) peroxide. Sections mounted on gelatin-coated slides Materials and Methods were dehydrated through graded alcohols into xylene and left overnight Animals, Male goldenhamsters (Mesocricetus auratus, LVG-outbred), before being rehydrated to distilled water and immersed in 0.2% (w/v) obtained from Lakeview, Charles River at IO weeks of age, were housed osmium tetroxide solution (2 min) to intensify the DAB reaction. His- individually and their wheel-running activity recorded.