Supporting Material

A Unique Metastasis Signature Enables Prediction of Tumor Relapse in Early Stage

Hepatocellular Carcinoma Patients

Stephanie Roessler1, Hu-Liang Jia2, Anuradha Budhu1, Marshonna Forgues1, Qing-Hai Ye2, Ju-

Seog Lee3, Snorri S. Thorgeirsson3, Zhongtang Sun4, Zhao-You Tang2, Lun-Xiu Qin2,*, Xin Wei

Wang1,*

1Laboratory of Human Carcinogenesis and 3Laboratory of Experimental Carcinogenesis,

National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; 2Liver Cancer

Institute & Zhongshan Hospital, Fudan University, Shanghai, China; 4National Laboratory of

Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing, China

*Correspondence should be addressed to: Dr. Wang, Laboratory of Human Carcinogenesis,

National Cancer Institute, NIH, 37 Convent Drive, Building 37, Room 3044A, Bethesda, MD

20892, USA; Email: [email protected]; or Dr. Qin at Liver Cancer Institute & Zhongshan Hospital,

Fudan University, 180 Feng Lin Road, Shanghai 200032, China; Email: qin.lunxiu@zs-

hospital.sh.cn

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Supplemental Figure Legends

Figure S1 Analysis of the sensitivity and specificity of AFP and BCLC staging as predictor for survival by Receiver Operating Characteristic (ROC) curves. (A) ROC curve applying AFP with a cutoff of 300ng/ml (N=238) and (B) ROC curve applying BCLC staging comparing stages

0and A versus B and C (N=225). Both ROC curves have been obtained using the LCI cohort.

AUC; area under the curve.

AB Sensitivity Sensitivity

AUC = 0.594 AUC = 0.743 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0

1-Specificity 1-Specificity

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Figure S2 Combination of survival risk prediction applying the Compound Covariate Predictor

(CCP) and AFP (300 ng/mL cutoff) to stratify patients into four subgroups. (A) The Kaplan-

Meier curves show overall survival of the LCI cohort (N = 238) and (B) LEC cohort (N = 104) sub-grouped by survival risk prediction and AFP (cutoff 300 ng/mL).

AB Signature AFP Signature AFP good prognosis low good prognosis low good prognosis high good prognosis high poor prognosis low poor prognosis low poor prognosis high poor prognosis high 100 100

80 80

60 60

40 40 Overall survival (%) 20 Log rank p=0.0077 Overall survival (%) 20 Log rank p=0.0054 p-trend=0.0012 p-trend=0.0007 0 0 0204060 0204060 Time (Months) Time (Months)

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Figure S3 Patient stratification using survival risk prediction and BCLC staging. Shown are

Kaplan-Meier curves of the LCI cohort (N = 225) by sub-grouping according to CCP class prediction of good or poor prognosis and BCLC stage 0-A or B-C.

Signature BCLC good prognosis 0-A poor prognosis 0-A good prognosis B-C poor prognosis B-C 100

80

vival (%) 60 r

40 all su r

Ove 20 Log rank p<0.0001 p-trend=0.7423 0 0204060 Time (Months)

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Supplemental Table 1: Significantly different between 10 cases which

presented with tumor thrombi in the major branches of the portal vein at surgery (PT) and 10

cases which were metastasis-free HCC patients (PN) at the time of surgery and at follow-up.

Rank Symbol Description Map p-value FDR Mean PN Mean PT Fold (PN/PT)

1 ENO2 Enolase 2 (gamma, 12p13 0.0000002 0.0018 0.5325 1.3140 0.4053 neuronal) 2 CENPE Centromere protein E, 4q24-q25 0.0000007 0.0032 0.8352 1.6465 0.5072 312kDa 3 LIMK1 LIM domain 1 7q11.23 0.0000013 0.0040 0.5751 0.9229 0.6231 4 LRP6 Low density lipoprotein 12p11-p13 0.0000031 0.0071 0.3257 0.6943 0.4691 receptor-related protein 6 5 RAB8A RAB8A, member RAS 19p13.1 0.0000047 0.0086 0.5478 0.9535 0.5745 oncogene family 6 ITGA9 Integrin, alpha 9 3p21.3 0.0000074 0.0095 0.6838 1.1673 0.5858 7 FZD2 Frizzled homolog 2 17q21.1 0.0000088 0.0095 0.5705 0.8768 0.6506 (Drosophila) 8 VAMP3 Vesicle-associated 1p36.23 0.0000115 0.0095 0.6135 1.1078 0.5538 membrane protein 3 (cellubrevin) 9 RPS6KA4 Ribosomal protein S6 11q11-q13 0.0000122 0.0095 1.9033 0.9983 1.9066 kinase, 90kDa, polypeptide 4 10 G3BP2 GTPase activating 4q21.1 0.0000125 0.0095 0.6200 1.1066 0.5603 protein (SH3 domain) binding protein 2 11 GTF2H1 General transcription 11p15.1-p14 0.0000154 0.0108 0.7152 1.0817 0.6612 factor IIH, polypeptide 1, 62kDa 12 RAB3A RAB3A, member RAS 19p13.2 0.0000185 0.0108 0.5943 1.0837 0.5484 oncogene family 13 U2AF1 U2 small nuclear RNA 21q22.3 0.0000195 0.0108 1.3551 0.8734 1.5515 auxiliary factor 1 14 MATK Megakaryocyte- 19p13.3 0.0000201 0.0108 0.6176 0.9872 0.6256 associated 15 RGS20 Regulator of G-protein 8q 0.0000212 0.0108 0.6813 0.9836 0.6927 signaling 20 16 MDFI MyoD family inhibitor 6p21 0.000023 0.0110 0.4494 0.7660 0.5867 17 ENO3 enolase 3 (beta, muscle) 17p13.2 0.0000263 0.0120 0.3960 0.8688 0.4559 (ENO3)

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18 HOXB13 Homeobox B13 17q21.2 0.0000309 0.0134 0.3600 0.9187 0.3919 19 GALK1 1 17q24 0.00003350.0139 7.6805 0.6903 11.1268 20 TBC1D16 TBC1 domain family, 17q25.3 0.0000377 0.0145 2.6300 1.3388 1.9645 member 16 21 N4BP1 NEDD4 binding protein 1 16q12.1 0.0000381 0.0145 1.3587 0.7892 1.7216 22 ZNF646 Zinc finger protein 646 16p11.2 0.0000437 0.0153 0.5131 0.8797 0.5833 23 VPS41 Vacuolar protein sorting 7p14-p13 0.0000475 0.0153 0.6539 1.0007 0.6535 41 homolog (S. cerevisiae) 24 AK3 3 9p24.1-p24.3 0.000048 0.0153 9.6368 0.6717 14.3472 25 ALDH3B1 Aldehyde 11q13 0.0000483 0.0153 0.5757 0.9428 0.6106 dehydrogenase 3 family, member B1 26 EPB42 Erythrocyte membrane 15q15-q21 0.0000485 0.0153 0.6149 0.9590 0.6412 protein band 4.2 27 STOML1 Stomatin (EPB72)-like 1 15q24-q25 0.0000585 0.0164 0.7584 1.0928 0.6941 28 JMJD8 Jumonji domain 16p13.3 0.0000589 0.0164 3.9184 0.6203 6.3168 containing 8 29 PDK1 Pyruvate dehydrogenase 2q31.1 0.0000598 0.0164 0.7159 1.0749 0.6660 kinase, isozyme 1 30 PLD2 Phospholipase D2 17p13.1 0.00006090.0164 0.6624 1.0160 0.6520 31 CRISPLD2 Cysteine-rich secretory 16q24.1 0.0000611 0.0164 1.7451 1.0211 1.7089 protein LCCL domain containing 2 32 STUB1 STIP1 homology and U- 16p13.3 0.0000702 0.0177 0.6793 1.0113 0.6717 box containing protein 1 33 PMAIP1 Phorbol-12-myristate- 18q21.32 0.0000736 0.0177 0.6512 1.3115 0.4965 13-acetate-induced protein 1 34 BASP1 Brain abundant, 5p15.1-p14 0.0000771 0.0177 0.6211 0.9019 0.6887 membrane attached signal protein 1 35 TSHZ1 teashirt zinc finger 18q22.3 0.0000774 0.0177 0.6041 1.0979 0.5503 homeobox 1 (TSHZ1) 36 CDH13 cadherin 13, H-cadherin 16q23.3 0.0000787 0.0177 0.6963 1.1259 0.6185 (heart) (CDH13) 37 CHGB chromogranin B 20p12.3 0.0000806 0.0177 0.6110 1.0829 0.5643 (secretogranin 1) (CHGB) 38 DTYMK Deoxythymidylate kinase 2q37.3 0.0000819 0.0177 0.9487 1.4553 0.6519 (thymidylate kinase) 39 GPD2 glycerol-3-phosphate 2q24.1 0.0000836 0.0177 0.6775 1.1494 0.5894 dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein

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40 JOSD1 Josephin domain 22q13.1 0.0000852 0.0177 0.6400 0.9708 0.6592 containing 1 41 ZNF605 Zinc finger protein 605 12q24.33 0.0000855 0.0177 5.3170 0.8934 5.9517 42 SLC20A2 Solute carrier family 20 8p12-p11 0.0000891 0.0181 0.6414 0.9874 0.6496 (phosphate transporter), member 2 43 ASPH Aspartate beta- 8q12.1 0.0001049 0.0207 0.6908 1.5576 0.4435 hydroxylase 44 LOC100131096 Hypothetical protein 17q25.3 0.0001066 0.0207 1.4685 1.0013 1.4667 LOC100131096 45 PCF11 PCF11, cleavage and 11q13 0.0001217 0.0231 1.6973 0.9399 1.8059 factor subunit, homolog (S. cerevisiae) 46 HCG2P7 HLA complex group 2 6p21.3 0.0001285 0.0235 1.0582 2.8493 0.3714 pseudogene 7 47 CYTH3 Cytohesin 3 7p22.1 0.00013540.0237 0.7575 1.0666 0.7102 48 HIPK1 Homeodomain 1p13.2 0.0001371 0.0237 1.7646 1.0890 1.6205 interacting 1 49 CALCOCO2 Calcium binding and 17q21.32 0.0001375 0.0237 0.6552 1.1220 0.5839 coiled-coil domain 2 50 COPA Coatomer protein 1q23-q25 0.0001437 0.0238 0.7118 0.9870 0.7212 complex, subunit alpha 51 FEN1 Flap structure-specific 11q12 0.0001619 0.0253 0.8765 1.4441 0.6069 endonuclease 1 52 NCAPD3 Non-SMC condensin II 11q25 0.0001654 0.0253 0.8594 1.2348 0.6960 complex, subunit D3 53 GYS1 glycogen synthase 1 19q13.3 0.0001677 0.0253 0.5870 0.8693 0.6753 (muscle) (GYS1) 54 ADD2 adducin 2 (beta) (ADD2) 2p13.3 0.0001691 0.0253 0.5375 0.7876 0.6825 55 LOC151162 Hypothetical LOC151162 2q21.3 0.0001692 0.0253 1.2420 0.8113 1.5308 56 SART1 Squamous cell 11q13.1 0.0001698 0.0253 0.5796 1.0725 0.5404 carcinoma antigen recognized by T cells 57 LRPPRC Leucine-rich PPR-motif 2p21 0.0001719 0.0253 1.9349 1.0121 1.9118 containing 58 CDCA4 Cell division cycle 14q32.33 0.00018 0.0260 1.5567 1.0511 1.4811 associated 4 59 UBXN4 UBX domain protein 4 2q21.3 0.0001849 0.0260 1.8655 1.3591 1.3725 60 SH3GLB1 SH3-domain GRB2-like 1p22 0.000185 0.0260 1.5280 1.0428 1.4653 endophilin B1 61 GCLC glutamate-cysteine 6p12.1 0.0001899 0.0260 0.5178 1.0439 0.4960 , catalytic subunit (GCLC)

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62 PPAPDC1B Phosphatidic acid 8p12 0.0001911 0.0260 2.1450 1.1317 1.8954 phosphatase type 2 domain containing 1B 63 RFC5 Replication factor C 12q24.23 0.0001987 0.0261 0.7963 1.4212 0.5603 (activator 1) 5, 36.5kDa 64 ATF2 Activating transcription 2q32 0.0002021 0.0261 0.6488 0.9308 0.6971 factor 2 65 MVK 12q24 0.0002021 0.0261 0.7510 1.1508 0.6526 66 RHAG Rh-associated 6p21.1-p11 0.0002033 0.0261 0.6750 0.9787 0.6897 glycoprotein 67 HMMR Hyaluronan-mediated 5q33.2-qter 0.0002069 0.0262 0.5535 0.9817 0.5638 motility receptor (RHAMM) 68 SELENBP1 Selenium binding 1q21-q22 0.0002178 0.0272 0.5533 0.9586 0.5772 protein 1 69 SPG11 Spastic paraplegia 11 15q14 0.0002222 0.0274 1.4905 1.0254 1.4535 (autosomal recessive) 70 CENTD3 Centaurin, delta 3 5q31.3 0.000228 0.0276 1.2394 0.9406 1.3177 71 LOC643733 Hypothetical LOC643733 11q22.3 0.0002317 0.0276 1.4349 0.9845 1.4575 72 GABRE Gamma-aminobutyric Xq28 0.0002328 0.0276 0.7223 1.0503 0.6878 acid (GABA) A receptor, epsilon 73 DTYMK Deoxythymidylate kinase 2q37.3 0.0002466 0.0281 0.5597 0.9413 0.5946 (thymidylate kinase) 74 WWTR1 WW domain containing 3q23-q24 0.0002491 0.0281 1.2836 0.9356 1.3719 transcription regulator 1 75 IVNS1ABP Influenza virus NS1A 1q25.1-q31.1 0.0002492 0.0281 1.9103 1.1425 1.6720 binding protein 76 ALDH3B2 Aldehyde 11q13 0.0002683 0.0297 0.6659 0.9046 0.7362 dehydrogenase 3 family, member B2 77 PRR3 Proline rich 3 6p21.33 0.0002767 0.0297 0.6923 0.9260 0.7476 78 CES1 Carboxylesterase 1 16q13-q22.1 0.0002769 0.0297 0.1937 0.9321 0.2078 (monocyte/macrophage serine esterase 1) 79 KIAA0319L KIAA0319-like 1p34.2 0.000282 0.0298 1.5935 1.1826 1.3474 80 CLCN7 Chloride channel 7 16p13 0.00028380.0298 0.8227 1.3057 0.6300 81 SPP1 secreted 4q22.1 0.0002872 0.0298 1.0377 3.2956 0.3149 phosphoprotein 1 (osteopontin, bone sialoprotein I, early T- lymphocyte activation 1) (SPP1) 82 SPTBN2 Spectrin, beta, non- 11q13 0.0002933 0.0299 0.6270 0.9541 0.6572 erythrocytic 2 83 CTRB1 Chymotrypsinogen B1 16q23-q24.1 0.000298 0.0299 0.7337 1.0220 0.7179

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84 UGT8 UDP glycosyltransferase 4q26 0.0002984 0.0299 1.2759 0.7454 1.7117 8 85 KHK Ketohexokinase 2p23.3 0.0003163 0.0305 0.6428 0.9337 0.6884 () 86 PITX2 Paired-like 4q25-q27 0.0003164 0.0305 0.7543 1.0832 0.6963 homeodomain 2 87 TACC1 Transforming, acidic 8p11 0.0003173 0.0305 1.7334 0.9687 1.7894 coiled-coil containing protein 1 88 ATP6V1G2 ATPase, H+ transporting, 6p21.3 0.0003228 0.0307 1.3505 0.9404 1.4361 lysosomal 13kDa, V1 subunit G2 89 AP1S1 Adaptor-related protein 7q22.1 0.000327 0.0308 0.6694 1.0160 0.6589 complex 1, sigma 1 subunit 90 IRF2 Interferon regulatory 4q34.1-q35.1 0.0003361 0.0310 0.5250 0.8903 0.5896 factor 2 91 ACBD5 Acyl-Coenzyme A 10p12.1 0.0003492 0.0310 1.5216 0.9952 1.5290 binding domain containing 5 92 YRDC YrdC domain containing 1p34.3 0.0003539 0.0310 1.6871 0.9792 1.7229 (E. coli) 93 EFTUD1 Elongation factor Tu GTP 15q25.2 0.0003588 0.0310 1.5895 1.0685 1.4877 binding domain containing 1 94 GTF2H4 General transcription 6p21.3 0.0003604 0.0310 0.6392 0.9039 0.7071 factor IIH, polypeptide 4, 52kDa 95 DNAJC9 DnaJ (Hsp40) homolog, 10q22.2 0.0003782 0.0317 2.2358 1.4314 1.5620 subfamily C, member 9 96 RPA2 Replication protein A2, 1p35 0.0003895 0.0319 0.9905 1.4698 0.6739 32kDa 97 CD247 CD247 molecule 1q22-q23 0.0003913 0.0319 0.5863 0.8543 0.6863 98 YES1 V-yes-1 Yamaguchi 18p11.31- 0.0004059 0.0328 0.6414 0.8840 0.7256 sarcoma viral oncogene p11.21 homolog 1 99 TRIM33 Tripartite motif- 1p13.1 0.0004106 0.0329 1.8324 1.0957 1.6723 containing 33 100 PDCD11 Programmed cell death 10q24.33 0.0004167 0.0330 1.4049 0.9330 1.5058 11 101 IL2RB Interleukin 2 receptor, 22q13|22q1 0.0004189 0.0330 0.6748 1.0006 0.6744 beta 3.1 102 MOAP1 Modulator of apoptosis 14q32 0.0004367 0.0338 1.4773 0.9035 1.6352 1 103 SLTM SAFB-like, transcription 15q22.1 0.0004446 0.0338 0.6749 1.1396 0.5922 modulator (SLTM) 104 CD37 CD37 molecule 19q13.3 0.00044790.0338 0.5415 0.7400 0.7317

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105 C14orf102 14 open 14q32.11 0.0004481 0.0338 1.1354 0.8396 1.3523 reading frame 102 106 MMP9 Matrix metallopeptidase 20q11.2- 0.0004486 0.0338 0.5573 0.9274 0.6009 9 (gelatinase B, 92kDa q13.1 gelatinase, 92kDa type IV collagenase) 107 PLA2G6 Phospholipase A2, group 22q13.1 0.0004691 0.0351 0.5451 0.7945 0.6860 VI (cytosolic, calcium- independent) 108 ARPP-21 Cyclic AMP-regulated 3p22.3 0.0004755 0.0353 1.1971 0.7983 1.4995 phosphoprotein, 21 kD 109 SPOCK3 Sparc/osteonectin, cwcv 4q32.3 0.0004809 0.0354 0.7255 1.1730 0.6185 and kazal-like domains proteoglycan (testican) 3 110 HSD3B1 hydroxy-delta-5-steroid 1p12 0.0005254 0.0384 0.6486 0.9018 0.7193 dehydrogenase, 3 beta- and steroid delta- 1 (HSD3B1) 111 KCNQ1 potassium voltage-gated 11p15.5 0.0005372 0.0389 0.6725 1.0366 0.6488 channel, KQT-like subfamily, member 1 (KCNQ1) 112 PDLIM1 PDZ and LIM domain 1 10q22-q26.3 0.0005425 0.0390 1.6464 1.0051 1.6380 113 SLC12A8 Solute carrier family 12 3q21.2 0.0005473 0.0390 1.6459 1.1211 1.4681 (potassium/chloride transporters), member 8 114 CSF3R Colony stimulating 1p35-p34.3 0.000551 0.0390 0.7841 1.0312 0.7604 factor 3 receptor (granulocyte) 115 CDS1 CDP-diacylglycerol 4q21.23 0.0005602 0.0393 0.7906 1.1651 0.6786 synthase (phosphatidate cytidylyltransferase) 1 116 LPL Lipoprotein lipase 8p22 0.00057410.0397 0.7476 1.0611 0.7045 117 LOC202451 Hypothetical protein 6q23.3 0.0005936 0.0404 1.5491 1.0206 1.5178 LOC202451 118 LPHN1 latrophilin 1 (LPHN1) 19p13.12 0.00059650.0404 0.6893 0.9806 0.7030 119 AMIGO1 Adhesion molecule with 1p13.3 0.0005978 0.0404 1.2479 0.7798 1.6002 Ig-like domain 1 120 AKR1C4 Aldo-keto reductase 10p15-p14 0.0006057 0.0406 0.6409 2.1071 0.3041 family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4)

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121 PYGB Phosphorylase, 20p11.2- 0.0006134 0.0406 0.8246 1.2315 0.6696 glycogen; brain p11.1 122 TRMU TRNA 5- 22q13 0.00063 0.0414 1.4941 0.9475 1.5769 methylaminomethyl-2- thiouridylate methyltransferase 123 RAD50 Homo sapiens RAD50 5q31.1 0.0006387 0.0416 0.5494 1.0029 0.5478 homolog (S. cerevisiae) (RAD50) 124 MAP3K6 Mitogen-activated 1p36.11 0.000649 0.0420 0.7763 1.0819 0.7175 protein kinase kinase kinase 6 125 CRIP1 Cysteine-rich protein 1 14q32.33 0.0006554 0.0421 1.5051 1.0100 1.4902 (intestinal) 126 PRUNE Prune homolog 1q21 0.0006623 0.0423 1.5021 1.1210 1.3399 (Drosophila) 127 NR1D2 Nuclear receptor 3p24.2 0.0006695 0.0424 0.6621 1.2757 0.5190 subfamily 1, group D, member 2 128 GCNT2 Glucosaminyl (N-acetyl) 6p24.2 0.0006762 0.0424 0.6209 0.8545 0.7266 2, I- branching (I blood group) 129 SUPT7L Suppressor of Ty 7 (S. 2pter-p25.1 0.000678 0.0424 1.3977 0.9410 1.4854 cerevisiae)-like 130 CHEK1 CHK1 checkpoint 11q24-q24 0.0006963 0.0432 0.8346 1.2821 0.6510 homolog (S. pombe) 131 NFKB1 Nuclear factor of kappa 4q24 0.000702 0.0433 0.7160 1.0435 0.6862 light polypeptide gene enhancer in B-cells 1 132 SERPINB6 Serpin peptidase 6p25 0.000706 0.0433 0.6323 1.3243 0.4775 inhibitor, clade B (ovalbumin), member 6 133 PS1TP4 HBV preS1- 2 0.0007309 0.0442 1.4181 0.9339 1.5185 transactivated protein 4 134 LTF Lactotransferrin 3p21.31 0.0007398 0.0442 0.2594 0.8031 0.3230 135 FANCC Fanconi anemia, 9q22.3 0.0007459 0.0442 0.6229 0.9152 0.6807 complementation group C 136 NUP205 Nucleoporin 205kDa 7q33 0.0007478 0.0442 0.7413 1.0871 0.6819 137 ZC3H12A Zinc finger CCCH-type 1p34.3 0.0007502 0.0442 1.4556 0.8893 1.6368 containing 12A 138 GRHL2 grainyhead-like 2 8q22.3 0.0007513 0.0442 2.0703 1.0225 2.0246 (Drosophila) (GRHL2) 139 RPS3 Ribosomal protein S3 11q13.3- 0.0007644 0.0447 1.3445 0.9819 1.3692 q13.5 140 RFTN1 Raftlin, lipid raft linker 1 3p24.3 0.0007707 0.0448 0.6942 0.9486 0.7317

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141 UNC119B Unc-119 homolog B (C. 12q24.31 0.0007795 0.0449 0.7964 1.3038 0.6108 elegans) 142 NOL7 Nucleolar protein 7, 6p23 0.0007827 0.0449 1.4966 1.0962 1.3653 27kDa 143 LANCL2 LanC lantibiotic 7q31.1- 0.0008018 0.0452 1.5114 0.8982 1.6827 synthetase component q31.33 C-like 2 (bacterial) 144 COL4A5 collagen, type IV, alpha 5 Xq22.3 0.0008283 0.0458 0.6416 0.9983 0.6426 (Alport syndrome) (COL4A5) 145 FUT8 Fucosyltransferase 8 14q24.3 0.0008319 0.0458 0.8567 1.2204 0.7020 (alpha (1,6) fucosyltransferase) 146 TMEM194A Transmembrane protein 12q13.3 0.0008322 0.0458 1.0806 2.0211 0.5346 194A 147 RAB28 RAB28, member RAS 4p15.33 0.0008469 0.0463 1.0619 1.5011 0.7074 oncogene family 148 MTUS1 Mitochondrial tumor 8p22 0.0008576 0.0466 1.0429 0.7265 1.4356 suppressor 1 149 SUV420H1 Suppressor of 11q13.2 0.0008796 0.0474 0.9489 1.4204 0.6680 variegation 4-20 homolog 1 (Drosophila) 150 C2orf3 Chromosome 2 open 2p11.2-p11.1 0.0008846 0.0474 0.8364 1.3274 0.6301 reading frame 3 151 CCR3 Chemokine (C-C motif) 3p21.3 0.0008901 0.0474 0.7396 1.2055 0.6135 receptor 3 152 ZNF415 Zinc finger protein 415 19q13.41 0.0008939 0.0474 1.3887 0.8830 1.5727 153 TGOLN2 Trans-golgi network 2p11.2 0.0009068 0.0477 0.5030 1.0356 0.4857 protein 2 154 EMD Emerin Xq28 0.000909 0.0477 0.5848 1.0877 0.5377 155 ANKRD1 Ankyrin repeat domain 1 10q23.31 0.0009227 0.0481 1.3867 0.7864 1.7633 (cardiac muscle) 156 GUCA2B Guanylate cyclase 1p34-p33 0.0009359 0.0485 0.6202 0.9328 0.6649 activator 2B (uroguanylin) 157 LY6E Lymphocyte antigen 6 8q24.3 0.0009448 0.0486 0.5382 0.8159 0.6596 complex, locus E 158 ABL2 V-abl Abelson murine 1q24-q25 0.0009482 0.0486 0.6571 0.9694 0.6778 leukemia viral oncogene homolog 2 (arg, Abelson-related gene) 159 TRAP1 TNF receptor-associated 16p13.3 0.0009543 0.0487 0.6500 1.0557 0.6157 protein 1 160 MYD88 Myeloid differentiation 3p22 0.0009693 0.0492 0.5008 0.8739 0.5731 primary response gene (88) 161 USP12 Ubiquitin specific 13q12.13 0.0009835 0.0496 0.6015 0.8947 0.6723

12 peptidase 12

13