View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by UCL Discovery 2.4. STRUCTURAL TOXICITY / 2.4.2. HYPERTROPHY In Stem Cell-Derived Models in Toxicology Book Janos Kriston-Vizi1, Sian E. Harding2, Gábor Földes2,3 1MRC Laboratory for Molecular Cell Biology, University College London, London, United Kingdom 2 National Heart and Lung Institute, Imperial College London, London, United Kingdom 3 Heart and Vascular Center, Semmelweis University, Budapest, Hungary Running head: hypertrophy models of hPSC-CM Number of words: Number of figures: 5 Correspondence: Gabor Foldes, MD PhD National Heart and Lung Institute Imperial College London Imperial Centre for Experimental and Translational Medicine Hammersmith Campus Du Cane Road London W12 0NN United Kingdom Email:
[email protected] and Janos Kriston-Vizi, PhD Email:
[email protected] 2 1. Abstract Human pluripotent stem cells (hPSC) are investigated as a source of au- thentic human cardiac cells for drug discovery and toxicological tests. Cell-based assays performed using an automated fluorescence imaging platform and high content analysis are valuable in characterizing hyper- trophic states that may be induced in hPSC-derived cardiomyocytes upon exposure to cardiotoxic compounds. In high-purity populations of hPSC- derived cardiomyocytes loaded with cell tracer probes and other cell mark- ers, detailed hypertrophic profiles can be assessed based on information captured at cellular and subcellular levels. Key Words: human pluripotent stem cells, cardiomyocyte, hypertrophy, automated high content imaging 2. Introduction. Cardiac hypertrophy is the abnormal enlargement of the heart muscle, resulting from a thickening of cardiomyocytes and changes in other heart muscle components, such as extracellular matrix, and can lead to heart failure.