Product datasheet Anti- E/PPIE Antibody (monoclonal, 4I9) Catalog Number: M08021 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-Cyclophilin E/PPIE Antibody (monoclonal, 4I9) Name PPIE Source Mouse IgG2b Species Reactivity human, mouse, rat Tested Application WB, IHC-P, ICC/IF, FCM Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human Cyclophilin E/PPIE recombinant protein (Position: M1-V301). Purification protein G purified. Observed MW 35KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an which in humans is encoded by the PPIE gene on 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on , has been identified.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-Cyclophilin E/PPIE Antibody (monoclonal, 4I9) Catalog Number: M08021 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. Western blot analysis of anti- PPIE analysis of anti- PPIE antibody (M08021).The antibody (M08021).The sample well of each lane sample well of each lane was loaded with 50ug of was loaded with 50ug of sample under reducing sample under reducing conditions. conditions. Lane 1: human HEK293 Lane 1: rat heart tissue whole cell lysates, lysates, Lane 2: human K562 Lane 2: rat brain tissue whole cell lysates, lysates, Lane 3: human PC-3 Lane 3: rat lung tissue whole cell lysates, lysates, Lane 4: human CACO-2 Lane 4: mouse heart whole cell lysates. tissue lysates, Use mouse anti- PPIE 1:1000, probed with a Lane 5: mouse brain goat anti- mouse IgG- tissue lysates, HRP secondary antibody. Lane 6: mouse lung tissue The signal is developed lysates, using an Enhanced Lane 7: RH35 whole cell Chemiluminescent lysates, detection (ECL) kit Lane 8: HEAP1/6 whole (Catalog # EK1001). A cell lysates. specific band was Use mouse anti- PPIE detected for PPIE at 1:1000, probed with a approximately 35KD. The goat anti- mouse IgG- expected band size for HRP secondary antibody. PPIE is at 35KD. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PPIE at approximately 35KD. The expected band size for PPIE is at 35KD.

Figure 3. IHC analysis Figure 4. IHC analysis using anti- PPIE antibody using anti- PPIE antibody (M08021). detected in (M08021). detected in paraffin-embedded paraffin-embedded section of human breast section of human lung tissue. cancer tissue. Biotinylated goat anti- Biotinylated goat anti- mouse IgG was used as mouse IgG was used as secondary antibody. The secondary antibody. The

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-Cyclophilin E/PPIE Antibody (monoclonal, 4I9) Catalog Number: M08021 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

tissue section was tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC) (Catalog # (SABC) (Catalog # SA1021) with DAB as the SA1021) with DAB as the chromogen. chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

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