A4b3. CERTIFICATE OF TRANSGENIC EMBRYO PRODUCTION FROM CELL LINE Page ___ of ___

Breed:______Cell Line IDentification:______Sex: ❑ M ❑ F

Production of cell line must have been documented and reported to record agency on Form A4b1 (Certificate of Source ofN uclear DNA - Cell Line Identification)

Identification of Animal from which Oocytes were aspirated:

Name:______No.: ______Breed: _____ Oocyte Recovery Date: ______CC Year Month Day OR Oocyte IdentificationN o: ______Date Enucleated: ______taken from form A4b2 Year Month Day Date of Nuclear Transfer: ______Year Month Day Passage Number: ______

T total Viable Embryos Produced: ______

No Frozen: ______

F freeze Date: ______Year Month Day N no. Transferred: ______

Signature:______Firm: ______Leader of Embryo Production Team ET Code: ______Phone: ( ) ______

C4. CERTIFICATE OF EMBRYO FREEZING OF TRANSGENIC EMBRYOS (see reverse side for coding instructions) Type of Container: Straw ____ other ____ E ach container labeled to show firm code, breed, reg. no. of donor, freeze date and straw no. Cane Straw No. Embryos/ No. X Trypsin Code Code Zona No. No. Straw Washed Treated Stage Quality Intact Cell Line ID Comments ______

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Time from recovery to onset of freezing ______(hrs.) Cryprotectant and concentration, equilibration, final molality and cooling procedure

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How Frozen: Seed Temp. ______Cooling Rate ______Plunge Temp. ______other ______

Recommended method of thawing and dilution ______

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Signature ______firm ______Practitioner or Leader of the Embryo Production Team Freezing the Embryos

ET Code ______Phone ( ) ______D CR CO BC BP AN Ovine BS SB BU BN BL BH BR BO BX BD BG BB BM BF BE BA A AK AF AB AN Bo TO AL Caprine HA LA Y Porcine Stage ofDevelopment BL AB AP A Equine if oocytes are collected from a registered/identified female, precede registration number with the A4b3. Use thefollowing theembryo, codestodescribe identifythebreed,andmonth inalldates. C4. Y O A S No. vine he Certificate of Certificate the - - - - - a frino ------ankole - afrikaner - abondance - aberdeenAngus - a - t - alpine - - landrace - - - arabian - appaloosa - - expandedHatchedBlastocyst 9 8 expandedBlastocyst 7 6 Blastocyst early 5 4 Morula early 3 2 1

Damara Corriedale Columbia Border Cheviot Blackhead Persian Brown Swiss() Brown Swiss() Braunvieh Brangus Braler Br Brahman Braford Boran Blonde D’Aquitaine Belgian Blue Beefmaster Beef F Beefalo Barzona Hampshire Yorkshire Belgian Saddlebred American oggenburg yrshire ahmental of each embryo with container labeling as set forth within the Certificate, alongwiththeaccuracy ofallotherinformation. withintheCertificate, withcontainerlabelingassetforth of eachembryo isattestingtotheaccuracyA4b1 andtheprocedurerecordsetforth ofallinformation whensigningthisform. line. of Certificates more or one Complete the country from which embryos are exported, with the appropriate fee. with theappropriate areexported, fromwhichembryos the country in respectiveorganization the breed to submitted be is forexport application special embryo a exported, are frozenembryos When be registeredorofficiallyidentified. responsibleagency for new the of identification the recording be owner followed. recording or organization breed respective the of requirements the that important is ownership,it changes embryo frozen a When resulting animalscanberegisteredorofficiallyidentified. Onecopy shouldbeprovided totheowner. organization.respective the by required as agency recording or association breed appropriate of Certificate complete the of copy One Registration/Identification. (cc).code country letter three (ISO) embryos are frozen.embryos Hatched Blastocyst Blastocyst Morula 2- to12-cell Unfertilized Stage riesian The leader of the embryo production team responsible for identifying the breed, the cell line, the oocyte batch number taken from mbryo Embryo GD GA DF FB FR FL FA ER FP DZ DR DE RW DJ DB CA CH CB CN CP BZ SA A HA FN DP DR DO HU DM HK HA CL PC LC DU he practitioner or leader of the embryo production team signing the certificate is attesting to the identification The practitioner production team signing the certificate or leader of the embryo G - - - F - F - F leckvieh - F lamand - F ringer - E ast FlemishRedPied - E ------angora - - finnishL andrace ------lacombe - - Galloway (dairy) Galloway (beef) Drakensberger Dexter Devon Danish Red& White Danish Jersey Danish Black & White Charolais Charbray Canadienne Campine RedPied Bonsmara Saanen Hampshire Dorper Dormer Dorset Hunter Dohne Merino Hackney (Pony) Hackney (Horse) Clydesdale Poland Chine Duroc F riesian (Dutch) riesian riesian (Belgian) riesian ribourg reezing of reezing CONDITIONS FORCOMPLETINGEMBRYO CERTIFICATES his would normally betheresponsibilityofownerThis wouldofrecord. normally description ofcodes.description Edition, Chapter9,for Refer toIETSManual, Code 4. Code 3. Code 2. Code 1. quality ofembryos he person signing the form is certifying that such female was identified with her Certificate of female Certificate such her that with was identified certifying is form the signing person The T T ransgenic ransgenic mbryo Embryo f excellentorGood Dead ordegenerating Poor air LM LU LR MA KB JE IN IB HY HO SH HP HH HC GZ GU GR GI GV GS PN PE PL MN BG CH CW NC SO MT BK ME LI LE IL T mbryo Production Embryo mbryos will be completed with the Certificate of Certificate the with completed be will Embryos ransfer of ransfer limousin - luing - - - - - induBrasil - ibage ------n. Cheviot Country - - - - - l incoln - l eicester - i le deF - Maine-Anjou Lincoln Red Kobe (Wagyu) Jersey lberta Hybrid) (Alberta Hybrid Holstein Highland) Highland (Scotch Hereford (polled) Hereford (horned) Hays Converter Guzera Guernsey Groninger Gir Gascone Pinto Percheron Palomino Morgan Boer Cashmere Chester White Spotted Montadale Berkshire Merino Third rance T ransgenic F rom Cell rom mbryos, with Certificate of Certificate with Embryos, X SEXED SEMEN other biopsies. Use commentcolumntodescribe U - Sex by Biopsy Undetermined M F D - Divided N - NotManipulated MANIPULATION OFEMBRYOS RN RP WW RD RB AR RA PZ PR PI P NR NM NG NE MG MO MI ME MR - F - Maleby Biopsy - F SN SI SE QH WS TM PE SP O SR SB RM RA LN KR A X emale emale by Biopsy ine for each production of embryos from one cell one from embryos of production each for Line his mayThis beforerequired be can animals resulting ------norwegian Red - normande - nguni - nelore ------t - - - o xford - - - - - laMancha - - Red Holstein Danish Red) Red Dane(RedDanish, Red Brangus Red Angus Ranger Pinzgauer Pie Rouge Piedmont Parthenais Murray Grey Montbeliard Meuse RhineIssel Standardbred Shire Shetland Quarter Horse Quarter Pietrain Persian Shropshire Romnelet Rambouillet Kalahari Reds Kalahari amworth (Speckled/Skilder) Y -Male nternational International Standards his record may be required before required be may record This mbryo Production, wheneverProduction, Embryo F reezing, is submitted to the to submitted is reezing, XX WF WB TU TL TG TA SX DS SM SI IS SP SS MS SE SG SA R LB L WD VR SU ST MM WE TH TW SF SG NU W O

- - - t - t - t - t ------large White (British) - largeBlack (British) ------thoroughbred - t - - - nubian - Crossbreds West FlemishRed Welsh Black Sussex South Devon Simmental Simbrah (Illawarra) Shorthorn Shorthorn (polled) Shorthorn Shorthorn (beef-Scotch) Shorthorn Shorthorn (milking) Shorthorn Senapol Santa Gertudis Salers Rotbunte White Dorper Van Rooy Suffolk Southdown S. MuttonMerino African Welsh Suffolk Punch uli exas Longhorn asmanian Grey arentaise Sa ennessee Walking vanna Goat A aP July June May April March F no December oC November October September January fe ebruary a ugust Organization JY JN MY MR DE SE J A U

IETS 23 3/06