Anti-KLF5 Antibody (ARG42734)

Product datasheet [email protected]

ARG42734 Package: 50 μl anti-KLF5 antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes KLF5

Tested Reactivity Hu

Tested Application IHC-P, WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name KLF5

Antigen Species Human

Immunogen Synthetic peptide of Human KLF5.

Conjugation Un-conjugated

Alternate Names Basic transcription element-binding protein 2; Transcription factor BTEB2; Krueppel-like factor 5; BTEB2; BTE-binding protein 2; Intestinal-enriched krueppel-like factor; GC-box-binding protein 2; IKLF; CKLF; Colon krueppel-like factor

Application Instructions

Application table Application Dilution

IHC-P 1:100 - 1:300

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control WB: Jurkat and Human ovarian cancer IHC-P: Human ovarian cancer and Human colon cancer

Calculated Mw 51 kDa

Observed Size ~ 50 kDa

Properties

Form Liquid

Purification Affinity purification with immunogen.

Buffer PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol.

Preservative 0.05% Sodium azide

Stabilizer 40% Glycerol

Concentration 0.8 mg/ml www.arigobio.com 1/3 Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol KLF5

Gene Full Name Kruppel-like factor 5 (intestinal)

Background This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Function Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. [UniProt]

Cellular Localization Nucleus. [UniProt]

Images

ARG42734 anti-KLF5 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG42734 anti-KLF5 antibody (left) at 1:120 dilution, or the same antibody pre-incubated with synthetic peptide (right). (Original magnification: X200).

ARG42734 anti-KLF5 antibody WB image

Western blot: 40 µg of Jurkat and Human ovarian cancer lysates stained with ARG42734 anti-KLF5 antibody at 1:1700 dilution.

www.arigobio.com 2/3 ARG42734 anti-KLF5 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG42734 anti-KLF5 antibody (left) at 1:120 dilution, or the same antibody pre-incubated with synthetic peptide (right). (Original magnification: X200).