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Biomedical Research Products

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Biomedical Research Products Biomedical Research Products Biomedical Technologies • Lipoprotein Products • Cell Culture Products • Assay Kits & Components • Antibodies & Proteins • Biochemical Products www.alfa.com Where Science Meets Service Biomedical Technologies Biomedical Technologies, (BT) is now part of Alfa Aesar. BT has been manufacturing life science research products for more than 30 years, with a commitment to customer intimacy, high quality and reasonable prices. BT’s product line includes specialty proteins, cell culture additives and highly specific immunoassay tools which Alfa Aesar will continue to build upon in expanding its portfolio of unique biochemical products for the researcher. Alfa Aesar is a leading manufacturer of fine chemicals and catalysts used for research and development in a wide span of applications. Alfa Aesar is already known for providing researchers access to unparalleled expertise and supply of a wide variety of inorganic and organic chemicals used in both academia and industry. Alfa Aesar is committed to enhancing our offering in the biochemical arena through a selection of methodically chosen products that complement our Alfa Aesar Offers: current portfolio and build on our core competencies. ACS Reagents Amino Acids and Derivatives Protein research and characterization, all types of profiling Antibiotics tools, and certain separation technologies are amongst such Antibodies products. Together, Alfa Aesar and BT continue to develop Apoptosis Reagents Buffers and manufacture bio products to exceed our customers’ Carbohydrates and Derivatives expectations. Cell Culture Reagents Chiral Compounds Click Chemistry Reagents Alfa Aesar currently offers a wide range of bio products in Electrophoresis Reagents the categories listed to the right. Enzymes Please visit www.alfa.com/biomedical-technologies for a full Growth Factors Natural Products range of products. Nucleosides and Nucleotides Peptides Signal Transduction Reagents Solvents, Acids, Bases Stains, Dyes and Indicators Vitamins Western Blot and ELISA Reagents One Source, Globally For more than 50 years, scientists have relied on Russia (distributor) Karlsruhe, Alfa Aesar to supply high purity raw materials for a Germany Kandel, Germany Heysham, variety of research and development England Royston, England Schiltigheim, applications. Today we offer over 46,000 products France Ward Hill, Massachusetts Seoul, Korea Reno, Nevada in stock, in sizes from gram-scale catalog items to (opening 2013) Yantai, Japan China (distributor) Shanghai, semi-bulk and bulk production quantities. With China Hyderabad, custom manufacturing capabilities to supply many India Taiwan Singapore (distributor) more specialized items, we are a one-stop source (distributor) for research chemicals, metals and materials. Alfa Aesar is a trusted, service-oriented company. Product quality is of paramount importance, but production expertise is just the beginning. Factors such as product availability, packaging and delivery are just as crucial to meeting and exceeding your expectations. Our established quality process ensures a high level of service and a promise of continually striving for perfection. Our dedicated teams, situated around the globe, are there to help you find the right products for your application when you need them. Our experience in manufacturing, sourcing and handling a wide range of chemicals enables us to respond quickly and efficiently to your needs. Global Inventory The majority of our 46,000 products are available in semi-bulk or bulk quantities from stock. Some are in regular production and are available in bulk for next day shipment. We also supply most products from stock in research and development quantities. Global Sourcing Knowledgeable chemical industry experts on our global sourcing team know what manufacturers match best to your needs. All Alfa Aesar vendors are assessed for quality and service to remain on our approved vendor list. We practice rigorous in-house product testing to ensure quality standards. www.alfa.com Cell Culture Products Cell Growth Factors Endothelial Mitogen (ECGS) Endothelial Mitogen (ECGS) is a partially purified preparation from bovine hypothalamus. Endothelial Mitogen was originally prepared to facilitate the growth and survival of human (or mammalian) vascular endothelial cells in vitro. ECGS is now used to improve growth, supplement and/or reduce serum concentrations in cultures of endothelial cells, Balb/C-3T3 cells and hybridoma cell cloning. Optimal concentration range Human Epidermal Growth Factor is from 25-200µg/ml and our ECGS does not contain Human Epidermal Growth Factor is recombinant DNA streptomycin which may be toxic to cells. ECGS is tested for produced from E. coli. It is structurally identical (53 amino endotoxins and mycoplasma. acids) to the natural Human EGF. Each lot is analyzed for radioreceptor binding, DNA replication (cell culture), immunological activity via RIA and absolute purity via Stock No. Name Size HPLC. J64516 Endothelial Cell Growth Supplement, 50mg bovine hypothalamus 10x50mg Stock No. Name Size Membrane filtered and packaged as a lyophilized solid J64012 Epidermal Growth Factor, human, 50µg Receptor Grade, 99% Epidermal Growth Factor J64008 Anti-human Epidermal Growth Factor 0.5ml Epidermal Growth Factor (EGF) is isolated from mouse antibody, from rabbit, whole serum submaxillary glands and is purified to electrophoretic Membrane filtered and packaged as a lyophilized solid homogeneity. Each lot is analyzed for radioreceptor binding, DNA replication (cell culture), immunological activity via RIA, and absolute purity via HPLC. Rat Epidermal Growth Factor EGF from human and mouse sources is significantly different Stock No. Name Size from rat EGF. Antibodies directed to human or mouse EGF J65329 Epidermal Growth Factor, mouse 100µg do not recognize rat EGF sufficiently to adequately assay, submaxillary gland, 99% 10x100µg localize or inhibit rat EGF containing samples. Receptor binding studies on rat cells are much “cleaner” using our Membrane filtered and packaged as a lyophilized solid Rat EGF reagents. Stock No. Name Size J64798 Epidermal Growth Factor, rat, Receptor 10µg Grade, 99% www.alfa.com Basic Fibroblast Growth Factor Bovine Pituitary Extract Basic FGF, a Class II heparin-binding growth factor, is Bovine Pituitary Extract (BPE) is an extract prepared from characterized by an isoelectric point 9.6 and elution bovine pituitary glands. BPE is used in conjunction with other from heparin-sepharose at 1.6-1.8M NaCl. bFGF has growth factors, hormones or media to reduce or eliminate similar biological activities to aFGF, but mitogenicity is not serum in the culturing of some epithelial cell types. BPE potentiated by heparin. Bovine bFGF is purified (~95%, SDS- has been effectively utilized in culturing normal human PAGE) from bovine pituitary glands via sepharose and ion epidermal keratinocytes and other epithelial cells. exchange chromatography. Human bFGF (MW 17.3kDa) is produced in E. coli and purified to near homogeneity. Stock No. Name Size J64417 Bovine Pituitary Extract 50mg Stock No. Name Size 10x50mg J65713 Fibroblast Growth Factor, basic, bovine, 95% 5µg Membrane filtered and packaged as a lyophilized solid 25µg J64811 Fibroblast Growth Factor, basic, human, 95% 10µg 50µg Platelet-Derived Growth Factor Platelet-Derived Growth Factor BB is recombinant DNA Membrane filtered and aseptically lyophilized produced and >97% pure via SDS gel electrophoresis. The acitivity of this product is similar to native h-PdGF in both Insulin-like Growth Factor receptor binding and in mitogen assays. Factor-I and Factor-II Insulin-like Growth Factors are recombinant DNA produced. Both have full biological Stock No. Name Size activity and are suitable for cell culture or receptor studies. J64812 Platelet-Derived Growth Factor-BB, 1µg human, Receptor Grade, 97% Stock No. Name Size J64081 Insulin-like Growth Factor-I, human, 97% 10µg J65170 Insulin-like Growth Factor-II, human, 97% 10µg Transforming Growth Factor-alpha Transforming Growth Factor-alpha is produced by recombinant DNA. Each lot (>98% pure, HPLC) is evaluated Anti-Nerve Growth Factor for EGF receptor binding. Anti-NGF is produced in rabbits from our mouse 2.5S NGF. The resulting antibody is purified by absorption. 90% Stock No. Name Size inhibition of NGF (10ng/ml) activity was demonstrated on J64478 Transforming Growth Factor-alpha, 2µg PC-12 cells at 1:100 dilution. Western blots of mouse 2.5S human, 98% NGF (5ng) are detectable at 1:20 dilution of our Packaged as a lyophilized solid anti-NGF using Goat Anti-Rabbit (GAR) alkaline phosphatase detection. Stock No. Name Size J64696 Anti-Mouse Nerve Growth Factor 2.5S 0.5ml antibody, from rabbit www.alfa.com Cell Culture Products Cell Growth Factors Transforming Growth Factor-beta 1 Transforming Growth Factor-beta 1 is isolated from blood bank produced human platelets. TGF-beta 1 is purified to >97% (SDS-PAGE). Stock No. Name Size J65044 Transforming Growth Factor-beta 1, human 5µg platelets, carrier free, 97% Cell Culture Additives Hybridoma Cell Growth Supplement Vascular Endothelial Growth Factor Hybridoma Cell Growth Supplement (HCGS) is a Vascular Endothelial Cell Growth Factor (h-VEGF) is an conditioned media additive which significantly improves angiogenesis factor produced by vascularized tissue. It is the cloning efficiency of hybridoma cells. HCGS eliminates unique in that its activity is specific for endothelial cells and the need for feeder cells. it has vascular permeability activity.
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