COURSE DESCRIPTION COURSE/SUBJECT: 3RD YEAR; FIRST SEMESTER DEGREE (S): PHARMACY MODALITY: ON-CAMPUS ACADEMIC YEAR: 2021/2022 FACULTY OF PHARMACY

Course Description / Academic year 2021-2022

1. COURSE/SUBJECT IDENTIFICATION

1.- COURSE/SUBJECT:

Name: Microbiology Code: 16648 Semester (s) when the course is taught: 1st Year (s) course is taught: 3rd Semester

Type: Compulsory subject ECTS of the course: 9 Hours ECTS: 30

Language: English Modality: On campus

Degree (s) in which the course is taught: Pharmacy

School which the course is taught: Pharmacy

2.- ORGANIZATION OF THE COURSE:

Department: Pharmaceutical and Health Science

Area of knowledge: Microbiology

2. LECTURERS OF THE COURSE/SUBJECT

1.-LECTURERS:

Responsible of the Course CONTACT Name: Dr. Estanislao Nistal Villán Phone (ext): 14714 Email: [email protected] Office: C216 Teaching and Research profile PhD in and Molecular Biology. Mount Sinai School of Medicine / New York University Associate Professor Research Lines Virus-Host interaction. induction and signalling. Oncolytic viruses. Two six-year research term recognized by CENAI.

Lecturer(s) CONTACT Name: Dr. Marina Robas Mora PhD Phone (ext): 14714 Email: [email protected] Office: B218 C Blng

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Course Description / Academic year 2021-2022

2.- TUTORIALS:

For any queries students can contact lecturers by e-mail, phone or visiting their office during the teacher’s tutorial times published on the students’ Virtual Campus.

3. COURSE DESCRIPTION

The Microbiology subject is a fundamental part in the education of pharmaceutical professionals. Knowing how to identify and interpret the main human pathogens is essential to be able to perform numerous job opportunities for a pharmacist. The skills acquired during in this course are basic in order to understand and be able to develop future knowledge in other subjects of the Grade of Pharmacy. During this course, we propose a review of the general Microbiology. During the first half, we will study, fundamental aspects of knowledge of the microbial world, the more common antibiotics, antivirals and antifungals, their mechanisms of action and resistance strategies of the microorganisms against them. In a second part, we will review characteristics of the main human pathogens, focusing the agenda on viruses, bacteria and fungi. The approach to the study of microorganisms will be attending the morphological characteristics, metabolic and molecular characteristics of the pathogens. We will study potential diseases produced by different infectious agents and their characteristics, describing the treatment of choice and possible strategies in the search for optimal treatment. Finally, it will be described methods of prophylaxis against different infectious agents.

4. COMPETENCIES

1.- COMPETENCIES

Code Basic and General Competencies CB1 Students have been shown to possess and understand knowledge in an area of study that is based on general secondary education at a level that, although it is supported by advanced textbooks, includes some issues of the forefront of knowledge of that field of study. CB2 Students apply their knowledge to their work or vocation in a professional manner, and have competences that can be demonstrated through devising and defending arguments or solving problems within their field of study. CB3 Students have the ability to collect and interpret relevant data (usually within their field of study), and make judgments after reflection regarding relevant issues of social, scientific or ethical nature. CB4 Students can communicate information, ideas, problems and solutions to both specialist and non-specialist audiences. CG09 To intervene in health promotion activities, disease prevention, at the individual, family and community level; with an integral and multiprofessional vision of the health-disease process.

Code Specific Competencies CE19 To estimate the biological risks associated with the use of substances and processes of laboratories involved. CE20 To understand the relationship between the life cycle of infectious agents and the properties of active components

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Course Description / Academic year 2021-2022

CE22 To know and understand the microbiological control of medications. CE24 To know the nature and behaviour of infectious agents.

2.- LEARNING OUTCOMES:

Code Learning outcomes RA73 To memorize the characteristics of microorganisms, especially pathogens for humans and microorganisms with potential biotechnological use, according to their physiological and biochemical differences, communicability, pathogenesis and treatment of choice in the field of taxonomic criteria management. RA74 To identify morphological, biological and epidemiological characteristics of the main parasites involved in human health. Associate the characteristics of major infectious diseases caused by parasites to their prevention and possible treatments. RA75 To correlate the host-microbe interactions, the importance of the nonspecific and specific defences against infection and the application of techniques immune to microbiological diagnosis. RA76 Isolate pure cultures of microorganisms, understanding their growth, both individually and in populations, their requirements and methods for targeted control of microbial metabolism, identification, characterization and epidemiological potential biotechnological and industrial use. RA77 Describe the main applications for diagnosis, characterization antibiotic resistance, industrial production and microbial genetics. Isolate pure cultures of microorganisms and parasites, microbial growth using aseptic techniques and the sterile concept.

5. LEARNING ACTIVITIES

1.- DISTRIBUTION OF STUDENTS` ASSIGNMENT:

Total hours of the course 270

Code Name On-campus hours AF1 Lectures (Antibiotic use; Fungi; introduction to virology and prions; 3 Industrial and Biotechnological Microbiology).. ECTS percentage 1,11%. Presentiality: 100% AF2 Seminars. ECTS percentage: 20,37 %.. Presentiality: 100% 55 AF3 Workshop - Seminar group - Academic Tutoring. . ECTS percentage: 2,59 7 %. Presentiality: 100% AF4 Practice. Laboratory practice. ECTS percentage: 13,33 %. Presentiality: 36 100% AF6 Evaluation activities. . ECTS percentage: 1,40%.. Presentiality: 100% 4 AF9 Tutorials. ECTS percentage: 1.11%. Presentiality: 100% 3 TOTAL Presence Hours 108

Code Name Not on- campus hours AF0 Self student work. ECTS percentage: 60 %. Presentiality: 0% 108

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Course Description / Academic year 2021-2022

2.- DESCRIPTION OF LEARNING ACTIVITIES:

Activity Definition

Lecture Learning activity that is developed in the classroom, that uses the descriptive methodology and prioritizes the teaching action of the lecturer Learning activity that is developed in the classroom-seminar, that under the Seminar guidance of the professor, encourages the cooperative learning among students and that is directed to the evaluation of cases and allows the study in detail. Learning activity that is developed in the classroom-workshop, which Workshop under the guidance of the teacher is aimed at individual or cooperative resolution of problems or exercises and the implementation of technical or artistic works. Learning activity that is developed in suitable facilities outside the Practice classroom, under the guidance of the class professor, encourages independent and / or cooperative learning of the student and, by appropriate design is aimed at the execution of a theoretical research or technical, practical or artistic work. Tutorials Formative activity in class or appropriate facilities such as teachers' offices in which doubts raised by students will be addressed personally. Others Setting examinations is within these activities.

6. ASSESMENT OF LEARNING

1.- CLASS ATTENDANCE:

 In order to be eligible for examination by continuous assessment students must attend at least 75% of scheduled class time (attendance sheets will be used). As students may be absent 25% of the classes, no attenuating circumstances will be accepted for absences.

 100% attendance at practical classes is required.

2.- ASSESMENT SYSTEM AND CRITERIA:

ORDINARY EXAMINATION (continuous assessment). Code Name Minimum Percentage score 1,2

SE1 Exam with questions and / or test 5 68 SE2 Written exam with exercises, problems and cases, etc. 5 10 SE3 On-site evaluation of various training activities 5 5 SE6 Work and reports that imply a reasoned interpretation, summary and 4 10 opinion by the student. SE7 Lab Notebook. 4 7

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Course Description / Academic year 2021-2022

EXTRAORDINARY EXAMINATION Code Name Minimum Percentage score 1,2 SE1 Exam with questions and / or test 5 78 SE2 Written exam with exercises, problems and cases, etc. 5 22

(1) In the exceptional case that the student cannot assist the classes, by previous permission by the professor, the student may take the ordinary examination, which will be evaluated according to the basic competences of the subject and will be considered as 75% of the final mark. Considering that, the remaining 25% will correspond to the laboratory practice score whose attendance is mandatory. (2) Minimum score to be able to apply to the percentages indicated.

Passing the practice class with a minimum score of 5 is mandatory to pass the subject.

The student that will not take either the final exam or the ordinary examination or the extraordinary exam, will be consider as “Not Shown” despite he or she may have performed the rest of the activities during the continuous evaluation during the course.

The student who does not attend the final exam in an extraordinary call will be qualified with "Not Shown", regardless of whether he or she has completed any academic activity of the continuous evaluation.

3.- DESCRIPTION OF ASSESSMENT CRITERIA:

Assessment criteria Definition

Examination of theme They are performed simultaneously and will be evaluated as indicated in type questions and / the tables above. or test (Liberatory Students who achieve a score ≥5 in liberatory examination will take the examination and exam only of the part of the subject which has not been evaluated. Those ordinary and students who do not achieve a score ≥5 in the liberatory exam must take extraordinary calls) the whole ordinary exam with all the matter. The score of this part of the exam must be compensated with the rest of the parts in the exam. Written exam with For the completion of the written laboratory practice exam it will be exercises, problems necessary to pass a practical Gram staining test. and cases, etc. (Written The mark of the practice exam must be ≥5 in order to pass the practices in Laboratory Evaluation). ordinary / extraordinary call. In case this average is not reached, the student will not pass the subject. On-site evaluation of The behavior, rigor, methodology and practical work of the student during various training the p0ractice will be evaluated. activities (evaluation of practice work) Work and reports that Individual work, evaluations and proposed tasks in class and / or in the imply a reasoned Virtual Campus. interpretation, summary and opinion by the student. Practices Laboratory notebook will be given to the professor in less than one week Notebook. after practice completion and it will be considered if the student gives it to the professor the same day of the exam. Presenting ne notebook is

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Course Description / Academic year 2021-2022

mandatory in order to correct the practice exam by the professor. The laboratory notebook will be corrected just once for every term exam.

7. COURSE PROGRAMME

1.- COURSE PROGRAMME:

THEORETICAL:

THEMATIC I. HISTORICAL INTRODUCTION. IMPORTANCE AND USES OF MICROBIOLOGY. Lesson 1. Microbiology and microorganisms. History of Microbiology: First observations of microorganisms. Controversy over spontaneous generation. The microorganisms as etiological agents of infectious diseases. Birth of . Applications of Microbiology.

THEMATIC SECTION II. MORPHOLOGY AND STRUCTURE OF MICROBIAL . Lesson 2. Prokaryotic cell. Size, shape and bacterial groups. General structure of the prokaryotic cell. Lesson 3. Surface structures of the bacterial cell (I). Capsules and mucous layers: detection, structure, composition and function. The bacterial cell wall: structure, composition and function. Lesson 4. Surface structures of the bacterial cell (II). Plasma membrane: structure, composition and functions. Flagella and hair. Structure, composition and function. Lesson 5. Intracellular structures. Prokaryotic nuclear material. Extrachromosomal DNA. Intracytoplasmatic structures. Ribosomes. Inclusions and reserve substances. Bacterial endospores: structure and composition. Sporulation and germination of spores. Lesson 6. Eukaryotic microorganisms. Microscopic fungi: molds and yeasts. Protozoa. Microscopic algae. Fungi diversity. Fundaments in the identification of microscopic fungi. Levaduriform, filamentous and dimorphic fungi.

THEMATIC SECTION III. MORPHOLOGY, STRUCTURE AND REPRODUCTION OF VIRUS. Lesson 7. Nature of the virus. Viral particle characteristics: the nucleic acid, the capsid and the envelope. Overview of the multiplication of the virus. Oncogenic viruses. Culture methods, counting and identification. Bacteriophages.

THEMATIC SECTION IV. MICROBIAL NUTRITION AND METABOLISM. Lesson 8. Microbial Nutrition. Assimilation of carbon, nitrogen, sulfur and phosphorus. Trace. Organic growth factors. Classification of microorganisms as sources of carbon and energy. Lesson 9. Fermentation: Concept and types of fermentation. Lactic fermentation. Alcoholic fermentation. Other fermentation and applications. Lesson 10. Breath. Aerobic respiration. Pasteur effect. Anaerobic respiration.

THEMATIC V. MICROBIAL GROWTH AND CONTROL OF MICROORGANISMS. Lesson 11. Microbial growth. Measures of microbial growth. Nature and mathematical expression of growth. Growth curve in a non-renewed environment. Relationship between growth rate and concentration of nutrients. Continuous cultivation. Lesson 12. Chemical and physical factors that affect microbial growth. Temperature. Ph. Osmotic pressure. Water. Redox potential. Lesson 13. Microbiological control using physical and chemical agents. Temperature. Filtration. Radiation. Sterility control systems for raw materials and finished products. Microbiological control techniques in drug production processes. Disinfectants and antiseptics. Gas sterilization. Preservatives.

THEMATIC SECTION VI. MICROBIAL GENETICS. Lesson 14. The genetic code. Transcription and translation. Mutations. Mutation, phenotypic variation and selection. Mutation rate. Mutagenesis: spontaneous mutations and mutagenic agents. Screening and selection of mutants. Lesson 15. Plasmids. Plasmids properties and their biological significance. Mobile genetic elements: insertion sequences and transposons. Integrons. Lesson 16. Genetic transfer and bacterial recombination. Transformation. Discovery processing. Bacterial transformation mechanisms, transduction and conjugation. Conjugative mobilization of chromosomal genes. 7

Course Description / Academic year 2021-2022

Meaning of conjugation in nature.

THEMATIC SECTION VII. ANTIMICROBIAL USE IN THERAPY. Lesson 17. Origin of chemotherapeutic agents: antibiotics of biological origin, synthetic and semisynthetic. Antimicrobial spectrum. Bacteriostatic and bactericidal chemotherapeutic agents. Lesson 18. Antibiotics that act on the cell wall synthesis. β -lactams. Other antibacterials that affect the cell wall: Fosfomycin, Cycloserine, Vancomycin and Bacitracin. Resistance to these antibiotics. Lesson 19. Antibiotics inhibiting protein synthesis. Aminoglycosides. Tetracyclines. Chloramphenicol. Macrolides. Resistance to these antibiotics. Lesson 20. Antibacterial agents, which act on the synthesis of nucleic acids. Quinolones. Novobiocin. Ansamycins : Rifamycins . Actinomycin D. Metronidazole. Resistance to these antibiotics.. Lesson 21. Antibacterial affecting plasma membrane: Antibiotics polypeptide. Antimetabolites: Sulfa. Trimethoprim. Urinary antiseptics: Nitrofurans., Antimycobacterial agents: isoniazid, PAS and Dapsone. Antifungals. Antivirals. Lesson 22. Bacterial resistance to antibiotics. Origin of resistance CMI and Antibiogram. Resistance epidemiology.

THEMATIC SECTION VIII. INMUNOLOGY AND APPLIYED TECHNIQUES FOR DIAGNOSTICS AND IDENTIFICATION OF MICROORGANISMS. Lesson 23. Humoral and cellular . Constitutive and inducible defenses of the host. Humoral immunity and cellular immunity against microorganisms. Antigens and Antibodies. Antibody Production. Lymphocytes B. Clonal selection. Origin of antibody diversity. Primary response and secondary response. Monoclonal antibodies. The Major Histocompatibility Complex (MHC). T lymphocytes. Antigen presenting cells. Antigen processing and presentation. Cooperation between lymphocytes. Effector mechanisms of calcification of the immune response. Cellular cytotoxicity. Lesson 24. Applied Immunology Microorganism study based in immunologic techniques. Immune- precipitation, agglutination reactions, immunofluorescence, immunosorbent assay (ELISA). Antisera Neutralization assays using antibodies. Inactivated and attenuated vaccines. Antisera and Immunoglobulins.

THEMATIC SECTION IX. FUNDAMENTAL CONCEPTS AND THE STUDY OF THE INFECTIOUS PROCCESS. Lesson 25. Relationship between the host organism and the microbial world. Pathogenic, opportunistic and commensal microorganisms. The human microbiota. Pathogenicity and virulence. Microbial pathogenicity mechanisms. Fundamental concepts in epidemiology. Transmission and infectivity. Containment measures against infections. Lesson 26. Study of microorganisms based on molecular techniques. Use of PCR to diagnose infections. Genotyping. Next generation sequencing techniques. Southern and Northern blots. The use of mass spectrometry to identify microorganisms.

THEMATIC SECTION X. VIROLOGY.

Lesson 27. DNA viruses which infect humans (I). Family Poxviridae: smallpox. Herpesviridae family: Herpes -simplex, varicella-zoster, cytomegalovirus and Epstein-Barr Virus. Lesson 28. DNA viruses which infect humans (II). Adenoviridae family. Family Papovaviridae: papilloma virus and polyoma virus. Family Parvoviridae Lesson 29. DNA viruses which infect humans (III). Hepadnaviridae family: Hepatitis B. Lesson 30. RNA viruses which infect humans (I). Reoviridae family. Rotavirus. Family Picornaviridae. : and, human . Caliciviridae: norovirus. Lesson 31. RNA viruses which infect animal cells (II). Family : the influenza viruses. Paramyxoviridae: parainfluenza viruses, RSV and mumps. Morbillivirus: measles virus. Lesson 32 RNA viruses which infect humans (III). Family Rhabdoviridae: rabies virus. Family Togaviridae, genus Alphavirus: Chikungunya virus and genus Rubivirus: rubella virus. Family Flaviviridae: (HCV) genus flavivirus: Dengue (DV), West Nile(WNV), yellow fever (YFV) and Zika viruses. Lesson 33. RNA viruses which infect humans (IV). Family Coronaviridae; SARS and MERS. Family Filoviridae; Ebola and Marburg.. Family Bunyaviridae; Crimean-Congo hemorrhagic fever virus (CCHV). Family Arenaviridae: Lymphocytic choriomeningitis virus (LCMV).. Lesson 34. RNA viruses which infect humans (V). Family Retroviridae. Retrovirus to infect human cells. Human Immunodeficiency Virus (HIV).

THEMATIC SECTION XI. BACTERIAL TAXONOMY.

Lesson 35. Bacterial taxonomy. Fundamental domains and diversity in the microbial world. Taxonomy and 8

Course Description / Academic year 2021-2022 diversity of prokaryotes. The bacterial species concept. Basis of classification. Nomenclature. Identification methods: molecular and phenotypic. Lesson 36. Gram negative bacteria: Proteobacteria. Class α -proteobacteria. Order Rickettsiales. Family Rickettsiaceae. Gender Rickettsia. Study of typhus fever and rash. Family Brucellaceae. Genus Brucella. Study of brucellosis. Lesson 37. Class β-proteobacteria . Family Neisseriaceae. Genus Neisseria. Study of gonococcal and meningococcal infections. Vaccination against meningitis. Lesson 38. Class β-proteobacteria: Family Alcaligenaceae. Genus Bordetella. Study and pertussis vaccination. Family Burkholderiaceae. Opportunistic infections by Burkholderia cepacia. Lesson 39. Class γ -proteobacteria . Family Xanthomonadaceae. Gender Stenotrophomonas. Common infections. Family Francisellaceae. Gender Francisella. Tularemia. Family Legionellaceae. Genus Legionella. Legionellosis. Family Coxiellaceae. Genus Coxiella. Q fever Lesson 40. Class γ -proteobacteria. Family Pseudomonadaceae. Pseudomonas genus. Common infections. Family Moraxellaceae. Genres Moraxella and Acinetobacter. Lesson 41. Class γ -proteobacteria. Family Vibrionaceae. Género Vibrio. Study of cholera and vaccination. Family Aeromonadaceae. Genus Aeromonas. Common infections. Lesson 42. Class γ –proteobacteria. Family Enterobacteriaceae. Health significance. Genus Escherichia. Shigella. Klebsiella. Salmonella. Proteus. Enterobacter. Serratia. Proteus. Yersinia. Other Enterobacteriaceae. Lesson 43. Class γ –proteobacteria. Family Pasteurellaceae. Haemophilus genus. Respiratory tract infections and menigitis produced by H. influenzae. Lesson 44. Class δ -proteobacteria. Bdellovibrio. Myxobacteria. Class ε -proteobacteria. Genres Campylobacter and Helicobacter. Common infections. Lesson 45. Gram negative bacteria which are not Proteobacteria (I). Family Chlamydiaceae. Chlamydia genus. Infections they produce. Family Bacteroidaceae. Bacteroides genus. Common infections.. Lesson 46. Gram negative bacteria which are not Proteobacteria (II). Spirochetes. Family Spirochaetaceae. Genus Treponema. Syphilis Study. Genus Borrelia. Study of relapsing fever and Lyme disease. Family Leptospiraceae. Genus Leptospira. Study of leptospirosis. Lesson 47. Gram positive bacteria of low G+C. Firmicutes (I). Class "Clostridia". Clostridium genus. Tetanus, botulism and other related infectious diseases. Topic 48. Gram positive bacteria of low G+C. Firmicutes (II). Class "Bacilli", Genus Bacillus. Infections that occur. Genus Listeria. Study of listeriosis. Lesson 49. Gram positive bacteria of low G+ C. Firmicutes (III). Staphylococcus. Associated infectious diseases. Lesson 50. Gram positive bacteria of low G+C. Firmicutes (IV). Genres Enterococcus and Streptococcus. Common infections Lesson 51. Bacteria without a cell wall. Tenericutes. Class Mollicutes.Género Mycoplasma . Infections that occur. Genre Ureaplasma. Infections that occur. Lesson 52. Gram positive bacteria of high G+C content. Actinobacteria (I). Family Corynebacteriaceae . Genus Corynebacterium. Study of diphtheria. Lesson 53. Gram positive bacteria of high G+C content Actinobacteria (II). Family Mycobacteriaceae. Genus Mycobacterium. Study of tuberculosis and leprosy. Treatment, chemoprophylaxis and vaccination. Lesson 54. Gram positive bacteria of high G+C content Actinobacteria (III). Family Actinomycetaceae. Genus Actinomyces. Family Nocardiaceae. Genus Nocardia. Common infections. Family Bifidobacteriaceae . Gender Gardnerella.

THEMATIC SECTION XII. FUNGI WITH CLINICAL INTEREST.

Lesson 55: Cutaneneus and subcutaneous superfiicial mycoses. Genus Malassezia, Epidermophyton, Trichophyton and Microsporum. Systemic mycoses and fungal opportunistic mycoses. Genres Candida, Pneumocystis, Aspergillus, Cryptococcus, Histoplasma, Coccidioides , Blastomyces and Paracoccidiodes.

THEMATIC SECTION XII. INDUSTRIAL BIOTECHNOLOGY AND MICROBIOLOGY.

Lesson 56. Recombinant DNA Technology. Genetic engineering in microorganisms. Restriction endonucleases. Cloning vectors. Recombinant DNA extraction and introduction of genes in microorganisms. Detection and selection of recombinants. Industrial microbiology. Industrial fermentations. Immunotherapy. Uso of oncolytic viruses and bacteria..

Master seminars: 1.- Antibiotic use and antibiotic overuse. 2.- Introduction to the fungi world. 3.- Introduction to virology and prions. 4.- Industrial biotechnology and microbiology 9

Course Description / Academic year 2021-2022

Workshops: 1.- Clinical cases of infections caused by viruses, fungi and prions. 2.- Clinical cases of infections caused by bacteria.

PRACTICAL WORK PROGRAMME:

I.- Microbiology techniques. 1.- Sterilization and preparation of culture media. 1.1.- Sterilization. 1.2.- Culture media. 2.- Plating methods. 2.1.- Taking an inoculum from a liquid medium. 2.2.- Plating in liquid media. 2.3.- Taking inoculum from a solid medium. 2.4.- Plating in solid media. 3.- Microorganism culture. 3.1.- Culture of anaerobic microorganisms. 3.2.- Culture of anaerobic microorganisms. 4.- Microorganism observation. 4.1.- Observation of preparations without staining. 4.2.- Observation of stained preparations.

II. Microbiological analysis of the air. 1.- Taking samples. 2.- Interpretation and identification of isolated microorganisms.

III. Microbiological analysis of water. 1.- Detection and counting of coliforms. 1.1.- Presumptive test. 2.2.- Confirmatory test. 2.- Detection and counting of faecal streptococci or enterococci. 2.1.- Presumptive test. 2.2.- Confirmatory test. 3.- Detection and counting of sporulated anaerobic sulphate-reducing bacteria. 4.- Isolation of pathogenic enterobacteria. Salmonella and Shigella. 4.1.- Enrichment media. 4.2.- Selective media. 5.- Isolation of Pseudomonas. 6.- Isolation of sporulated bacteria. 6.1.- Isolation. 6.2.- Aerobic and anaerobic bacteria separation. 6.3.- Study of isolated bacteria. IV.- Microbiological analysis of the human urethral normal microbiota and their relationship with urinary tract infections (OTU). 1.- Taking samples. 2.- Plating samples. 3.- Interpretation of the cultures and identification of isolated microorganisms. V.- Microbiological analysis of pharyngeal exudate and human oral microbiota. 1.- Taking samples. 2.- Plating samples. 3.- Interpretation of the cultures and identification of isolated microorganisms.

VI.- Microbiological analysis of samples from eyes, ears, nose and skin. 1.- Analysis of samples coming from the eyes. 2.- Analysis of samples coming from the ears. 3.- Analysis of samples coming from the nose. 4.- Analysis of samples coming from the skin.

VII.- Bacteria identification test. 1.- Identification of Gram positive cocci. 2.- Identification of Gram positive rod-shaped bacteria. 3.- Identification of Gram negative rod-shaped bacteria. 3.1.- Identification test for enterobacteria. 3.2.- Identification test for Gram negative bacteria with oxidative or fermentative metabolism, 10

Course Description / Academic year 2021-2022

non enterobacteria.

VIII.- Study of the antibiotic sensitivity. 1.- Technique: Diffusion disk technique. 2.- Results interpretation.

IX.- Annex.

8. RECOMMENDED READING

1.- ESSENTIAL BIBLIOGRAPHY:

• Madigan MT, Martinko JM, Dunlap PV y DP Clark. 2015. Brock Biología de los Microorganismos. 13ª Edición, Pearson. • Prescott LM, Harley JP y DA Klein. 2009. Microbiología. 7ª Edición. Mc Graw-Hill Interamericana de España. • A. M. Skalka, Vincent R. Racaniello, S. Jane Flint, Glenn F. Rall. 2015. Principles of Virology, American Society for Mocrobiology Press.

2.- ADDITIONAL BIBLIOGRAPHY:

• Jennifer Louten. Essential Human Virology. Academic Press. Elsevier 2016. • Knipe DM, Howley PM, 2013, Fields virology. Sixth edition, 2013. Lippincott Williams & Wilkins. • Murray P R, Rosenthal KS y M A Pfaller. 2016. Microbiología Médica, 8ª Edición. Elsevier Mosby. • Tortora GJ, Funke BR y CL Case, 2009, Introducción a la Microbiología. 9ª Edición. Editorial Médica Panamericana • Abbas AK, Lichtman AH y JS Pover. 2008. Inmunología Celular y Molecular. 6ª Edición. Harcourt Brace de España SA • Roitt IM y PJ Delves. 2008. Inmunología Fundamentos. 11ª Edición. Editorial Médica Panamericana.

4.- WEB RESOURCES :

• Podcast This week in virology: by Dr. Vincent Racaniello. In addition to the podcast file, in the blog, you can find other interesting apps and resources. Link: http://www.microbe.tv/twiv/ • Podcast This Week in Microbiology by Dr. Vincent Racaniello. In the podcast, you can find information about fundamental and applied microbiology released in recent publications. Link: http://www.microbe.tv/twiv/ •. Microbiology program by Enrique Láñez from Universidad de Granada: http://www.ugr.es/~eianez/Microbiologia/programa.htm • Microbiology in Spanish with interesting images and resources. Link: http://www.microbiologia.com.ar/ • Online Microbiology book with open access: http://gsbs.utmb.edu/microbook/ • Visit to the microbial world through Microbe Zoo: Link: http://commtechlab.msu.edu/sites/dlc-me/zoo/ • Virology in Spanish website: http://virologia.ua.es/ • David Ellis Mycology, with nice images and links: http://www.mycology.adelaide.edu.au/ • Immunology program by Dr. Enrique Láñez, from Universidad de Granada: Link: http://www.ugr.es/~eianez/inmuno/Programa97.htm • Taxonomic bacteria nomenclarure and information: http://www.bacterio.cict.fr/ • Antibiotic course 1.01 (in Spanish): Link: http://www.infecto.edu.uy/espanol/cursos/antibiotico/atbfa/frame.htm

9. ATTITUDE IN THE CLASSROOM

1.- REGULATIONS 11

Course Description / Academic year 2021-2022

Any irregular act of academic integrity (no reference to cited sources, plagiarism of work or inappropriate use of prohibited information during examinations) or signing the attendance sheet for fellow students not present in class will result in the student not being eligible for continuous assessment and possibly being penalized according to the University regulations. The students that use any non-authorized information during the exam will lose the possibility to pass the examination they are taking (ordinary and extraordinary). Cell phones will only be allowed when the professor gives explicit permission. It is not allowed to bring coats, bags and folders into the laboratory. The student will have available lockers in order to keep the material. The student will need to bring a lock.

10. EXCEPTIONAL MEASURES

Should an exceptional situation occur which prevents continuing with face-to-face teaching under the conditions previously established to this end, the University will take appropriate decisions and adopt the necessary measures to guarantee the acquisition of skills and attainment of learning outcomes as established in this Course Unit Guide. This will be done in accordance with the teaching coordination mechanisms included in the Internal Quality Assurance System of each degree.

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