AGP1/Alpha 1 Acid BioAssay™ ELISA Kit (Human)

Catalog number 396557 Supplier United States Biological

Alpha-1-acid glycoprotein, or orosomucoid, is a relatively abundant plasma synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180aa. AGP is considered an acute phase reactant; its plasma levels increase several fold during acute . It belongs to the family and binds numerous basic and neutral lipophilic drugs and hormones. And this is located in 9q34.3-qter. Sample Type Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Human AGP1/alpha 1 acid glycoprotein. Sensitivity <150pg/ml Specificity Recognizes natural and recombinant Human AGP1/alpha 1 acid glycoprotein. There is no detectable cross-reactivity with other relevant . Test Principle This Human AGP1/alpha 1 acid glycoprotein ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A specific for AGP1/alpha 1 acid glycoprotein has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q19-S201) and test samples are added to the wells, a biotinylated detection specific for AGP1/alpha 1 acid glycoprotein is added subsequently and then followed by washing with PBS or TBS buffer. -Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human AGP1/alpha 1 acid glycoprotein amount of sample captured in plate. Kit Components 96-well plate precoated with anti-Human ORM1 , 1x plate Lyophilized recombinant Human ORM1 standard, 2x100ng/tube Biotinylated anti-Human ORM1 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Applications E Storage 4°C/-20°C Tests <150pg/ml Sensitivity 50-100ul Sample Volume Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Detection Range Colorimetric Antigen Modification Human Antigen Source 96

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